Rh5BG030100

Polyadenylate-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005574
Physical Location & Seq
Reverse (-)
2123829 .. 2127387
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG030100.1

Sequence Viewer

Length: 1383 bp
ATGACACGAAACGGATTTACGTGCCTTTTCCAAAAATCCCTAGTCCCCTTTAAATCTGTCTCTCACTCGTCACATTCTATCTTCTGTCTCAGTGTCTCCCTTCCTCCACGGTTAGGGTTTCCTTCTCTCTCCTCTCTCTCTAACCCTAGATTCGTCGTCATCATGATGCAACCCGGCTCCGGTCTGGCTCCGTCGCAAATGGGCCAGCAGCCGGCCCAGCAATACGGCCAGCAGCCGCCCGCCGCCCAGCCCTACATGATGATGCCGCCTCCGCCTCCCGCCGTCCAGGCCCCGCCGATGTGGGCCCCGCAGTCGCACGTGGCCCCGCCGCAGCCGCAGCCGGCCCAGCCCACCAGCGCCGACGAGGTCCGCACGCTCTGGATCGGCGACTTGCAGTACTGGATGGAAGAGACCTATCTTTTCAGTATCTTCGCCAACACCGGCGAGGTTGTTAGTGTGAAAGTCATCCGGAATAAGCAAACTACTCAATCGGAGGGCTATGGGTTTATTGAGTTTGTTAATCGAGCTGCTGCAGAAAGAGTGCTTCAGACGTACAATGGTACCCCTATGCCAAATGGTGGCCAGCCTTTCAGATTGAACTGGGCATCTGCAGGGGAGAAGCGTTCTGATGATACGCCTGATTATACAATATTTGTGGGCGACTTGGCTGTGGATGTGACAGATTATATGCTACAAGAGATATTTAAGGCTCGCTATCCATCATGCAAGGGAGCCAAGGTTGTTATTGACAGACTTACCGGCCGCACCAAGGGATATGGGTTTGTTAAGTTTGGAGATGAGAGTGAACAGTTGCGTGCAATGACCGAGATGAATGGAGTTGTTTGCTCTTCACGGCCCATGCGAATTGGGCCAGCTGCAAACAAAAATGCTGGGGGTAATCAGCAATATTCGAAAGCTTCATATCAGAGTTCTCCAGGTGCAAAGAATGAGACCGATCCCAATAATACAACTATATTTGTTGGAAATCTGGATGCTAACGTTACCGATGATCATTTGAGAACAGTTTTTAGCCAGTATGGTGAGCTAGTTCATGTTAAGATACCAGCAGGCAAGCGATGTGGGTTTGTTCAGTTCGCAGACAGGAGCCGTGCTGAAGAGGCTTTGCAAGGATTAAATAATTCTCAGATTGGTGGACAAAATATCCGACTTTCTTGGGGTCGCAGTCCTTCAAACAAGCAGCAGGCTCAGGCAGATCCTAACCAATGGAATGCTGGCTATTATGGATATGCACCAACCCCCGAGGGATATGGATATGCCCAAGTATCCCAAGACCCTAACATGTACTATGGAGGGTATCCTGCTGGATATGGCAATTACCAGCAGTCACAGCAGCAACAGCAGCAGCAAGTGGGTTATAGTTGA

Protein Analysis

460

Amino Acids

50.65

Weight (kDa)

8.42

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 126 - 190 1.1e-12 RNA recognition motif
RRM_1 PF00076 217 - 286 5.2e-13 RNA recognition motif
RRM_5 PF13893 304 - 394 1.5e-07 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
RRM_occluded PF16842 324 - 390 3.7e-06 Occluded RNA-recognition motif
RRM_1 PF00076 325 - 389 1.7e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 560
AccB7I CCANNNNNTGG 1 cut(s) 578
AccIII TCCGGA 1 cut(s) 468
AclI AACGTT 1 cut(s) 999
AclWI GGATC 3 cut(s) 389, 950, 1208
AcoI YGGCCR 3 cut(s) 226, 580, 760
AcuI CTGAAG 2 cut(s) 530, 1134
AcvI CACGTG 1 cut(s) 319
AfaI GTAC 4 cut(s) 398, 554, 562, 1304
AfiI CCNNNNNNNGG 5 cut(s) 113, 179, 211, 578, 769
AflIII ACRYGT 1 cut(s) 1299
AgsI TTSAA 2 cut(s) 598, 1191
AjnI CCWGG 2 cut(s) 285, 934
AluBI AGCT 4 cut(s) 527, 875, 917, 1045
AluI AGCT 4 cut(s) 527, 875, 917, 1045
Alw26I GTCTC 5 cut(s) 64, 92, 100, 404, 944
AlwI GGATC 3 cut(s) 389, 950, 1208
Ama87I CYCGRG 1 cut(s) 1259
Aor13HI TCCGGA 1 cut(s) 468
ApaI GGGCCC 1 cut(s) 307
Asp718I GGTACC 1 cut(s) 560
AspLEI GCGC 1 cut(s) 359
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 1052
AsuII TTCGAA 1 cut(s) 911
AvaI CYCGRG 1 cut(s) 1259
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 307
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BalI TGGCCA 1 cut(s) 582
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 307
BbrPI CACGTG 1 cut(s) 319
BccI CCATC 2 cut(s) 397, 727
BceAI ACGGC 4 cut(s) 241, 266, 869, 1092
BciT130I CCWGG 2 cut(s) 287, 936
BciVI GTATCC 2 cut(s) 1294, 1326
BclI TGATCA 1 cut(s) 1009
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 5 cut(s) 64, 92, 100, 404, 944
BfaI CTAG 3 cut(s) 41, 147, 1046
BfmI CTRYAG 2 cut(s) 531, 609
BfoI RGCGCY 1 cut(s) 360
BfuI GTATCC 2 cut(s) 1294, 1326
BglI GCCNNNNNGGC 1 cut(s) 287
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 174, 287, 936
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 1259
BmiI GGNNCC 9 cut(s) 178, 189, 291, 305, 306, 324, 562, 733, 1106
BmrFI CCNGG 3 cut(s) 174, 287, 936
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 4 cut(s) 156, 252, 614, 982
BmuI ACTGGG 1 cut(s) 610
BpmI CTGGAG 1 cut(s) 918
Bpu10I CCTNAGC 1 cut(s) 1206
Bpu14I TTCGAA 1 cut(s) 911
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 2 cut(s) 21, 319
BsaI GGTCTC 2 cut(s) 404, 944
BsaJI CCNNGG 4 cut(s) 107, 735, 768, 1260
BsaWI WCCGGW 2 cut(s) 179, 468
BsaXI ACNNNNNCTCC 2 cut(s) 723, 753
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 179, 211, 578, 769
Bse118I RCCGGY 4 cut(s) 211, 340, 440, 758
Bse1I ACTGG 3 cut(s) 404, 605, 1033
Bse3DI GCAATG 1 cut(s) 825
BseAI TCCGGA 1 cut(s) 468
BseBI CCWGG 2 cut(s) 287, 936
BseDI CCNNGG 4 cut(s) 107, 735, 768, 1260
BseGI GGATG 4 cut(s) 408, 465, 679, 997
BseLI CCNNNNNNNGG 5 cut(s) 113, 179, 211, 578, 769
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 3 cut(s) 103, 1157, 1220
BseNI ACTGG 3 cut(s) 404, 605, 1033
BseRI GAGGAG 1 cut(s) 121
BseSI GKGCMC 1 cut(s) 307
BseX3I CGGCCG 1 cut(s) 760
BseYI CCCAGC 4 cut(s) 216, 246, 345, 890
Bsh1285I CGRYCG 1 cut(s) 763
BshNI GGYRCC 1 cut(s) 560
BsiEI CGRYCG 1 cut(s) 763
BsiHKCI CYCGRG 1 cut(s) 1259
BsiSI CCGG 7 cut(s) 174, 180, 212, 341, 441, 469, 759
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 5 cut(s) 113, 179, 211, 578, 769
BsmAI GTCTC 5 cut(s) 64, 92, 100, 404, 944
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 1234
Bso31I GGTCTC 2 cut(s) 404, 944
BsoBI CYCGRG 1 cut(s) 1259
Bsp119I TTCGAA 1 cut(s) 911
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 307
Bsp13I TCCGGA 1 cut(s) 468
Bsp143I GATC 4 cut(s) 381, 955, 1009, 1213
BspCNI CTCAG 3 cut(s) 102, 1156, 1219
BspEI TCCGGA 1 cut(s) 468
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 9 cut(s) 178, 189, 291, 305, 306, 324, 562, 733, 1106
BspMAI CTGCAG 2 cut(s) 535, 613
BspPI GGATC 3 cut(s) 389, 950, 1208
BspQI GCTCTTC 1 cut(s) 853
BspT104I TTCGAA 1 cut(s) 911
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 2 cut(s) 404, 944
BsrDI GCAATG 1 cut(s) 825
BsrFI RCCGGY 4 cut(s) 211, 340, 440, 758
BsrI ACTGG 3 cut(s) 404, 605, 1033
BssAI RCCGGY 4 cut(s) 211, 340, 440, 758
BssECI CCNNGG 4 cut(s) 107, 735, 768, 1260
BssMI GATC 4 cut(s) 381, 955, 1009, 1213
BssT1I CCWWGG 2 cut(s) 735, 768
Bst2UI CCWGG 2 cut(s) 287, 936
Bst4CI ACNGT 3 cut(s) 111, 810, 1024
Bst6I CTCTTC 3 cut(s) 402, 853, 1110
BstBAI YACGTR 2 cut(s) 21, 319
BstBI TTCGAA 1 cut(s) 911
BstDEI CTNAG 3 cut(s) 89, 1143, 1206
BstDSI CCRYGG 1 cut(s) 107
BstF5I GGATG 4 cut(s) 408, 465, 679, 997
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 4 cut(s) 384, 958, 1012, 1216
BstMAI GTCTC 5 cut(s) 64, 92, 100, 404, 944
BstMBI GATC 4 cut(s) 381, 955, 1009, 1213
BstMCI CGRYCG 1 cut(s) 763
BstNI CCWGG 2 cut(s) 287, 936
BstNSI RCATGY 1 cut(s) 1303
BstSCI CCNGG 3 cut(s) 172, 285, 934
BstSFI CTRYAG 2 cut(s) 531, 609
BstSLI GKGCMC 1 cut(s) 307
BstX2I RGATCY 1 cut(s) 1213
BstYI RGATCY 1 cut(s) 1213
BstZI CGGCCG 1 cut(s) 760
BsuI GTATCC 2 cut(s) 1294, 1326
BtgI CCRYGG 1 cut(s) 107
BtgZI GCGATG 1 cut(s) 1090
BtsCI GGATG 4 cut(s) 408, 465, 679, 997
BtsIMutI CAGTG 1 cut(s) 97
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 4 cut(s) 211, 340, 440, 758
Csp6I GTAC 4 cut(s) 397, 553, 561, 1303
CspCI CAANNNNNGTGG 4 cut(s) 636, 671, 1060, 1095
CviAII CATG 6 cut(s) 163, 256, 723, 859, 1052, 1300
CviQI GTAC 4 cut(s) 397, 553, 561, 1303
DdeI CTNAG 3 cut(s) 89, 1143, 1206
DpnI GATC 4 cut(s) 383, 957, 1011, 1215
DpnII GATC 4 cut(s) 381, 955, 1009, 1213
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 3 cut(s) 226, 580, 760
EagI CGGCCG 1 cut(s) 760
Eam1104I CTCTTC 3 cut(s) 402, 853, 1110
EarI CTCTTC 3 cut(s) 402, 853, 1110
EciI GGCGGA 1 cut(s) 261
EclXI CGGCCG 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 735, 768
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 2 cut(s) 404, 944
Eco47I GGWCC 1 cut(s) 367
Eco52I CGGCCG 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 530, 1134
Eco72I CACGTG 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 1259
EcoO109I RGGNCCY 2 cut(s) 289, 304
EcoRII CCWGG 2 cut(s) 285, 934
EcoT14I CCWWGG 2 cut(s) 735, 768
EcoT38I GRGCYC 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 735, 768
FaeI CATG 6 cut(s) 166, 259, 726, 862, 1055, 1303
FaqI GGGAC 1 cut(s) 29
FatI CATG 6 cut(s) 162, 255, 722, 858, 1051, 1299
FauI CCCGC 5 cut(s) 247, 286, 300, 315, 333
FbaI TGATCA 1 cut(s) 1009
FokI GGATG 4 cut(s) 415, 452, 686, 1004
FriOI GRGCYC 1 cut(s) 307
FspBI CTAG 3 cut(s) 41, 147, 1046
GlaI GCGC 1 cut(s) 358
GsaI CCCAGC 4 cut(s) 220, 250, 349, 894
GsuI CTGGAG 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 360
HapII CCGG 7 cut(s) 174, 180, 212, 341, 441, 469, 759
HhaI GCGC 1 cut(s) 359
Hin1II CATG 6 cut(s) 166, 259, 726, 862, 1055, 1303
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 915
HinfI GANTC 1 cut(s) 150
HpaII CCGG 7 cut(s) 174, 180, 212, 341, 441, 469, 759
HphI GGTGA 1 cut(s) 1052
Hpy166II GTNNAC 2 cut(s) 806, 1154
Hpy188I TCNGA 7 cut(s) 493, 549, 593, 628, 927, 1146, 1166
Hpy188III TCNNGA 4 cut(s) 163, 379, 469, 989
Hpy8I GTNNAC 2 cut(s) 806, 1154
Hpy99I CGWCG 3 cut(s) 158, 196, 365
HpyAV CCTTC 3 cut(s) 110, 132, 1197
HpyCH4III ACNGT 3 cut(s) 111, 810, 1024
HpyCH4IV ACGT 4 cut(s) 20, 318, 551, 999
HpyF3I CTNAG 3 cut(s) 89, 1143, 1206
HpySE526I ACGT 4 cut(s) 20, 318, 551, 999
Hsp92II CATG 6 cut(s) 166, 259, 726, 862, 1055, 1303
HspAI GCGC 1 cut(s) 357
Kpn2I TCCGGA 1 cut(s) 468
KpnI GGTACC 1 cut(s) 564
KroI GCCGGC 2 cut(s) 211, 340
KroNI GCCGGC 2 cut(s) 213, 342
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 381, 955, 1009, 1213
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 4 cut(s) 182, 193, 731, 1104
LweI GCATC 4 cut(s) 156, 252, 614, 982
MaeI CTAG 3 cut(s) 41, 147, 1046
MaeII ACGT 4 cut(s) 20, 318, 551, 999
MaeIII GTNAC 4 cut(s) 69, 676, 1000, 1344
MalI GATC 4 cut(s) 383, 957, 1011, 1215
MboI GATC 4 cut(s) 381, 955, 1009, 1213
MboII GAAGA 5 cut(s) 73, 419, 421, 840, 1127
MflI RGATCY 1 cut(s) 1213
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 3 cut(s) 864, 1138, 1333
MluNI TGGCCA 1 cut(s) 582
MmeI TCCRAC 2 cut(s) 961, 1189
Mox20I TGGCCA 1 cut(s) 582
MroI TCCGGA 1 cut(s) 468
MroNI GCCGGC 2 cut(s) 211, 340
MscI TGGCCA 1 cut(s) 582
MseI TTAA 6 cut(s) 51, 519, 705, 786, 1056, 1133
MslI CAYNNNNRTG 2 cut(s) 164, 260
Msp20I TGGCCA 1 cut(s) 582
MspA1I CMGCKG 1 cut(s) 875
MspI CCGG 7 cut(s) 174, 180, 212, 341, 441, 469, 759
MspR9I CCNGG 3 cut(s) 174, 287, 936
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 2 cut(s) 287, 936
NaeI GCCGGC 2 cut(s) 213, 342
NciI CCSGG 1 cut(s) 174
NdeII GATC 4 cut(s) 381, 955, 1009, 1213
NgoMIV GCCGGC 2 cut(s) 211, 340
NlaIII CATG 6 cut(s) 166, 259, 726, 862, 1055, 1303
NlaIV GGNNCC 9 cut(s) 178, 189, 291, 305, 306, 324, 562, 733, 1106
NmuCI GTSAC 3 cut(s) 69, 676, 1344
NspI RCATGY 1 cut(s) 1303
NspV TTCGAA 1 cut(s) 911
PagI TCATGA 1 cut(s) 162
PciI ACATGT 1 cut(s) 1299
PciSI GCTCTTC 1 cut(s) 853
PcsI WCGNNNNNNNCGW 1 cut(s) 859
PctI GAATGC 1 cut(s) 1234
PdiI GCCGGC 2 cut(s) 213, 342
PfeI GAWTC 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 365
PflMI CCANNNNNTGG 1 cut(s) 578
PmaCI CACGTG 1 cut(s) 319
PmlI CACGTG 1 cut(s) 319
Ppu21I YACGTR 2 cut(s) 21, 319
PscI ACATGT 1 cut(s) 1299
Psp1406I AACGTT 1 cut(s) 999
Psp6I CCWGG 2 cut(s) 285, 934
PspCI CACGTG 1 cut(s) 319
PspFI CCCAGC 4 cut(s) 216, 246, 345, 890
PspGI CCWGG 2 cut(s) 285, 934
PspN4I GGNNCC 9 cut(s) 178, 189, 291, 305, 306, 324, 562, 733, 1106
PspOMI GGGCCC 1 cut(s) 303
PstI CTGCAG 2 cut(s) 535, 613
PsuI RGATCY 1 cut(s) 1213
PsyI GACNNNGTC 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 875
RsaI GTAC 4 cut(s) 398, 554, 562, 1304
RsaNI GTAC 4 cut(s) 397, 553, 561, 1303
RseI CAYNNNNRTG 2 cut(s) 164, 260
SapI GCTCTTC 1 cut(s) 853
SaqAI TTAA 6 cut(s) 51, 519, 705, 786, 1056, 1133
Sau3AI GATC 4 cut(s) 381, 955, 1009, 1213
ScaI AGTACT 1 cut(s) 398
ScrFI CCNGG 3 cut(s) 174, 287, 936
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 4 cut(s) 156, 252, 614, 982
SfcI CTRYAG 2 cut(s) 531, 609
SfuI TTCGAA 1 cut(s) 911
SgrAI CRCCGGYG 1 cut(s) 440
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 164, 260
Sse9I AATT 3 cut(s) 864, 1138, 1333
SspI AATATT 2 cut(s) 651, 908
SspMI CTAG 3 cut(s) 41, 147, 1046
StyD4I CCNGG 3 cut(s) 172, 285, 934
StyI CCWWGG 2 cut(s) 735, 768
TaaI ACNGT 3 cut(s) 111, 810, 1024
TaiI ACGT 4 cut(s) 23, 321, 554, 1002
TaqI TCGA 2 cut(s) 523, 911
TaqII GACCGA 2 cut(s) 839, 968
TasI AATT 3 cut(s) 864, 1138, 1333
TatI WGTACW 2 cut(s) 396, 1302
TauI GCSGC 6 cut(s) 238, 245, 268, 331, 337, 765
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 6 cut(s) 51, 519, 705, 786, 1056, 1133
Tru9I TTAA 6 cut(s) 51, 519, 705, 786, 1056, 1133
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 3 cut(s) 69, 676, 1344
Tsp45I GTSAC 3 cut(s) 69, 676, 1344
TspDTI ATGAA 3 cut(s) 845, 909, 1040
TspGWI ACGGA 2 cut(s) 27, 180
TspRI CASTG 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 365
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 367
XceI RCATGY 1 cut(s) 1303
XcmI CCANNNNNNNNNTGG 1 cut(s) 1229
XspI CTAG 3 cut(s) 41, 147, 1046
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.