Rw2G018740
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
Physical Location & Seq
Forward (+)
24516740 .. 24525033
8294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018740.1

Sequence Viewer

Length: 1836 bp
ATGGTAATGTTGTTGAAGATGGAGCTACTATTTGCAGTTCTGGCACTTGCTAGCTTGCATTCTTTCGTATTGCCAGATCCACAAGGAGATGCACTGTATTCATTGAGGTCTTCATTGATTACTTCACCCAATCAGCTCAAGGACTGGAATCCCAACCAAGTCAATCCATGCACTTGGTCCAATGTGAATTGCGACACTGACAACAATGTCATTTCTGTAACATTGTCAAATATTGGATTTAATGGAACCTTATCCCCTAAAGTAGGAAACCTGAAAACTCTTCAAACACTTGCATTGCAAGGAAATGGCATAACTGGTGAGATACCAAAAGAGTTTGGAGATCTGACAAGTTTGGCCACCTTGAATTTGGAAAATAATAATTTAACTGGTGCAATACCATCTTCCCTTGGTAATCTGAAAAAGCTTCAATTCTTGACTTTGAGTCAAAACAATTTGAGCGGGAGCATCCCAGATTCACTTTCAAATCTTTCAACTTTGATTAATCTTCAGCTCGCCTTCAATGCTCTCAGTGGTCAAATACCTGAGAAGTTATTTCAGATTCAAAAATACAATTTTACAGGAAACAAACTAAACTGTGGCGTAAATCTTCCCCACAGTTGTGCATCTGATACTGATGTGTCTGGATCTTCAAATAAGCCAAAGCTTGGAATTATAGTTGGAGCTGTTGGAGGCTTCATCATTTTACTTTTTGGAGGCTTGGTTTACTTTGTTTGTAAGCGTAGACACAGAGGCTACAGGCGTGAAGTTTATGTAGATGTTGCAGGTGAAGTTGACCGAAGGATTGCATTCGGTCAGTTGAAAAGATTTTCGTGGAGGGAATTGCAGCTGGCTACAGACAACTTCAATGAGAACAATGTTCTAGGACAGGGGGGTTTTGGTAAAGTCTATAAAGGAGTGTTATCTGATACTACAAAAATCGCTGTTAAACGGCTGACCGATTATGAGAGTCCTGGGGGAGATGCAGCTTTTCAGCGTGAAGTTGAAATGATAAGTGTAGCTGTTCACAGGAACCTATTACGACTGATTGGATTTTGCACAACCCCAACAGAACGCCTTTTGGTGTATCCCTTCATGCAAAATTTAAGTGTTGCCTATCGTTTACGAGAGATTAAACCGGGGGAGCCTGTTTTGGATTGGCCTACAAGAAAGAGGGTGGCTTTAGGCACAGCCCGTGGCTTAGAGTACCTACATGAACATTGTAATCCTAAGATCATTCATCGAGATGTTAAGGCGGCTAATGTATTACTTGACGAAGATTTTGAAGCAGTTGTTGGTGATTTCGGCTTGGCAAAGTTGGTGGATGTTAGAAAGACTAATGTGACAACTCAAGTTCGTGGGACAATGGGCCACATAGCACCTGAATACTTGTCTACTGGGAAGTCATCAGAAAGGACGGATGTTTTTGGCTATGGGATTATGCTTTTGGAGCTTGTAACAGGACAGCGGGCAATTGATTTCTCACGATTGGAAGAAGAAGATGATGTTTTGTTGCTGGACCATGTCAAGAAGTTGGAAAGGGAAAAGAGACTGGATGCTATAGTAGATGGAAATCTGGATAGTAGTTACAACATCCAAGAGGTAGAAATGATGATCCAAGTTGCACTGCTTTGTACCCAAGGATCCCCTGAAGATCGTCCACTAATGTCGGAGGTGGTACGTATGCTGGAAGGAGAGGGTCTGGCTGAGCGGTGGGAAGAATGGCAGCATGTGGAAGTTACTCGCAGGCAAGAGTACGAGAGACTACAAAGGAGATTTGATTGGGGAGAAGACTCGGTTTATAACCAGGATGCTATTGAGTTATCTGGTGGACGATGA

Protein Analysis

611

Amino Acids

68.11

Weight (kDa)

5.74

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 65 2.3e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 83 - 184 5.7e-12 Leucine-rich repeat region
LRR_8 PF13855 117 - 176 4.3e-06 Leucine rich repeat
Pkinase PF00069 289 - 557 2.3e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 291 - 562 1.2e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1800
AarI CACCTGC 1 cut(s) 773
AasI GACNNNNNNGTC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 773
AccB7I CCANNNNNTGG 1 cut(s) 665
AccBSI CCGCTC 2 cut(s) 459, 1708
AccI GTMKAC 2 cut(s) 742, 1391
AciI CCGC 4 cut(s) 459, 1253, 1465, 1708
AclWI GGATC 5 cut(s) 71, 652, 1606, 1635, 1648
AcoI YGGCCR 1 cut(s) 354
AcsI RAATTY 2 cut(s) 364, 1099
AcuI CTGAAG 2 cut(s) 491, 1668
AfaI GTAC 4 cut(s) 1205, 1633, 1677, 1754
AfiI CCNNNNNNNGG 2 cut(s) 263, 665
AhdI GACNNNNNGTC 1 cut(s) 441
AjnI CCWGG 2 cut(s) 970, 1803
AjuI GAANNNNNNNTTGG 2 cut(s) 1275, 1307
AleI CACNNNNGTG 1 cut(s) 618
Alw26I GTCTC 2 cut(s) 1540, 1753
AlwI GGATC 5 cut(s) 71, 652, 1606, 1635, 1648
AoxI GGCC 3 cut(s) 354, 1157, 1366
ApeKI GCWGC 3 cut(s) 844, 983, 1723
ApoI RAATTY 2 cut(s) 364, 1099
ArsI GACNNNNNNTTYG 2 cut(s) 1262, 1294
AseI ATTAAT 1 cut(s) 501
AspS9I GGNCC 3 cut(s) 177, 1366, 1516
AsuC2I CCSGG 1 cut(s) 1137
AsuHPI GGTGA 4 cut(s) 117, 329, 797, 1307
AsuNHI GCTAGC 1 cut(s) 50
AvaII GGWCC 2 cut(s) 177, 1516
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BalI TGGCCA 1 cut(s) 356
BamHI GGATCC 1 cut(s) 1640
BbsI GAAGAC 2 cut(s) 102, 1794
BbvI GCAGC 3 cut(s) 856, 995, 1735
BccI CCATC 3 cut(s) 13, 406, 1559
BceAI ACGGC 1 cut(s) 965
BciT130I CCWGG 2 cut(s) 972, 1805
BciVI GTATCC 1 cut(s) 1095
BcnI CCSGG 1 cut(s) 1137
BcoDI GTCTC 2 cut(s) 1540, 1753
BfaI CTAG 2 cut(s) 51, 881
BfmI CTRYAG 3 cut(s) 754, 852, 1557
BfuAI ACCTGC 1 cut(s) 773
BfuI GTATCC 1 cut(s) 1095
BglII AGATCT 1 cut(s) 340
BisI GCNGC 4 cut(s) 845, 984, 1254, 1724
BlpI GCTNAGC 1 cut(s) 1704
BlsI GCNGC 4 cut(s) 846, 985, 1255, 1725
Bme1390I CCNGG 3 cut(s) 972, 1137, 1805
Bme18I GGWCC 2 cut(s) 177, 1516
BmeRI GACNNNNNGTC 1 cut(s) 441
BmgT120I GGNCC 3 cut(s) 177, 1366, 1516
BmiI GGNNCC 4 cut(s) 247, 1031, 1143, 1642
BmrFI CCNGG 3 cut(s) 972, 1137, 1805
BmrI ACTGGG 1 cut(s) 1404
BmsI GCATC 6 cut(s) 79, 474, 632, 970, 1543, 1798
BmtI GCTAGC 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 1404
BpiI GAAGAC 2 cut(s) 102, 1794
Bpu1102I GCTNAGC 1 cut(s) 1704
BpuEI CTTGAG 2 cut(s) 122, 1332
BpuMI CCSGG 1 cut(s) 1137
BsaAI YACGTR 1 cut(s) 1679
BsaBI GATNNNNATC 1 cut(s) 1569
BsaJI CCNNGG 5 cut(s) 406, 971, 1136, 1192, 1636
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 665
Bse1I ACTGG 5 cut(s) 149, 319, 391, 1399, 1554
Bse3DI GCAATG 1 cut(s) 293
Bse8I GATNNNNATC 1 cut(s) 1569
BseBI CCWGG 2 cut(s) 972, 1805
BseDI CCNNGG 5 cut(s) 406, 971, 1136, 1192, 1636
BseGI GGATG 6 cut(s) 465, 1327, 1423, 1558, 1590, 1813
BseJI GATNNNNATC 1 cut(s) 1569
BseLI CCNNNNNNNGG 2 cut(s) 263, 665
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 3 cut(s) 534, 541, 1695
BseNI ACTGG 5 cut(s) 149, 319, 391, 1399, 1554
BseXI GCAGC 3 cut(s) 856, 995, 1735
BshFI GGCC 3 cut(s) 356, 1159, 1368
BsiSI CCGG 1 cut(s) 1136
BslFI GGGAC 1 cut(s) 1372
BslI CCNNNNNNNGG 2 cut(s) 263, 665
BsmAI GTCTC 2 cut(s) 1540, 1753
BsmFI GGGAC 1 cut(s) 1372
BsmI GAATGC 2 cut(s) 58, 806
BsnI GGCC 3 cut(s) 356, 1159, 1368
Bsp143I GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
Bsp1720I GCTNAGC 1 cut(s) 1704
BspACI CCGC 4 cut(s) 459, 1253, 1465, 1708
BspANI GGCC 3 cut(s) 356, 1159, 1368
BspCNI CTCAG 3 cut(s) 535, 540, 1696
BspLI GGNNCC 4 cut(s) 247, 1031, 1143, 1642
BspMI ACCTGC 1 cut(s) 773
BspOI GCTAGC 1 cut(s) 54
BspPI GGATC 5 cut(s) 71, 652, 1606, 1635, 1648
BsrBI CCGCTC 2 cut(s) 459, 1708
BsrDI GCAATG 1 cut(s) 293
BsrI ACTGG 5 cut(s) 149, 319, 391, 1399, 1554
BssECI CCNNGG 5 cut(s) 406, 971, 1136, 1192, 1636
BssMI GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
BssT1I CCWWGG 2 cut(s) 406, 1636
Bst2UI CCWGG 2 cut(s) 972, 1805
Bst4CI ACNGT 3 cut(s) 96, 596, 617
Bst6I CTCTTC 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 1679
BstC8I GCNNGC 6 cut(s) 52, 56, 513, 849, 1467, 1745
BstDEI CTNAG 5 cut(s) 527, 543, 1198, 1227, 1704
BstDSI CCRYGG 1 cut(s) 1192
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 6 cut(s) 465, 1327, 1423, 1558, 1590, 1813
BstKTI GATC 7 cut(s) 79, 343, 647, 1233, 1614, 1643, 1654
BstMAI GTCTC 2 cut(s) 1540, 1753
BstMBI GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
BstMWI GCNNNNNNNGC 3 cut(s) 41, 521, 1447
BstNI CCWGG 2 cut(s) 972, 1805
BstNSI RCATGY 1 cut(s) 1730
BstSCI CCNGG 3 cut(s) 970, 1135, 1803
BstSFI CTRYAG 3 cut(s) 754, 852, 1557
BstSNI TACGTA 1 cut(s) 1679
BstV1I GCAGC 3 cut(s) 856, 995, 1735
BstV2I GAAGAC 2 cut(s) 102, 1794
BstX2I RGATCY 4 cut(s) 76, 340, 644, 1640
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 4 cut(s) 76, 340, 644, 1640
BsuI GTATCC 1 cut(s) 1095
BsuRI GGCC 3 cut(s) 356, 1159, 1368
BtgI CCRYGG 1 cut(s) 1192
BtsCI GGATG 6 cut(s) 465, 1327, 1423, 1558, 1590, 1813
BtsI GCAGTG 1 cut(s) 1622
BtsIMutI CAGTG 4 cut(s) 92, 195, 535, 1622
BveI ACCTGC 1 cut(s) 773
Cac8I GCNNGC 6 cut(s) 52, 56, 513, 849, 1467, 1745
Cfr13I GGNCC 3 cut(s) 177, 1366, 1516
Csp6I GTAC 4 cut(s) 1204, 1632, 1676, 1753
CspCI CAANNNNNGTGG 2 cut(s) 1299, 1334
CviAII CATG 5 cut(s) 168, 1093, 1211, 1520, 1727
CviQI GTAC 4 cut(s) 1204, 1632, 1676, 1753
DdeI CTNAG 5 cut(s) 527, 543, 1198, 1227, 1704
DpnI GATC 7 cut(s) 78, 342, 646, 1232, 1613, 1642, 1653
DpnII GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
DrdI GACNNNNNNGTC 1 cut(s) 206
DriI GACNNNNNGTC 1 cut(s) 441
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 285
Eam1105I GACNNNNNGTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 285
Eco105I TACGTA 1 cut(s) 1679
Eco130I CCWWGG 2 cut(s) 406, 1636
Eco47I GGWCC 2 cut(s) 177, 1516
Eco57I CTGAAG 2 cut(s) 491, 1668
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 970, 1803
EcoT14I CCWWGG 2 cut(s) 406, 1636
ErhI CCWWGG 2 cut(s) 406, 1636
FaeI CATG 5 cut(s) 171, 1096, 1214, 1523, 1730
FaqI GGGAC 1 cut(s) 1372
FatI CATG 5 cut(s) 167, 1092, 1210, 1519, 1726
FauI CCCGC 2 cut(s) 452, 1458
FblI GTMKAC 2 cut(s) 742, 1391
Fnu4HI GCNGC 4 cut(s) 845, 984, 1254, 1724
FokI GGATG 6 cut(s) 452, 1334, 1430, 1565, 1577, 1820
Fsp4HI GCNGC 4 cut(s) 845, 984, 1254, 1724
FspBI CTAG 2 cut(s) 51, 881
GluI GCNGC 4 cut(s) 845, 984, 1254, 1724
HaeIII GGCC 3 cut(s) 356, 1159, 1368
HapII CCGG 1 cut(s) 1136
Hin1II CATG 5 cut(s) 171, 1096, 1214, 1523, 1730
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HindIII AAGCTT 2 cut(s) 422, 662
HinfI GANTC 6 cut(s) 148, 442, 473, 559, 967, 1790
HpaII CCGG 1 cut(s) 1136
HphI GGTGA 4 cut(s) 117, 329, 797, 1307
Hpy166II GTNNAC 8 cut(s) 724, 743, 793, 1024, 1121, 1392, 1658, 1829
Hpy188I TCNGA 7 cut(s) 345, 417, 558, 628, 925, 1408, 1669
Hpy188III TCNNGA 6 cut(s) 433, 642, 1241, 1482, 1525, 1574
Hpy8I GTNNAC 8 cut(s) 724, 743, 793, 1024, 1121, 1392, 1658, 1829
HpyAV CCTTC 4 cut(s) 526, 792, 1099, 1682
HpyCH4III ACNGT 3 cut(s) 96, 596, 617
HpyCH4IV ACGT 1 cut(s) 1678
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 521, 1447
HpyF3I CTNAG 5 cut(s) 527, 543, 1198, 1227, 1704
HpySE526I ACGT 1 cut(s) 1678
Hsp92II CATG 5 cut(s) 171, 1096, 1214, 1523, 1730
Kzo9I GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
LmnI GCTCC 5 cut(s) 22, 462, 680, 1141, 1447
Lsp1109I GCAGC 3 cut(s) 856, 995, 1735
LweI GCATC 6 cut(s) 79, 474, 632, 970, 1543, 1798
MaeI CTAG 2 cut(s) 51, 881
MaeII ACGT 1 cut(s) 1678
MaeIII GTNAC 5 cut(s) 217, 1339, 1453, 1583, 1735
MalI GATC 7 cut(s) 78, 342, 646, 1232, 1613, 1642, 1653
MbiI CCGCTC 2 cut(s) 459, 1708
MboI GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
MfeI CAATTG 1 cut(s) 1470
MflI RGATCY 4 cut(s) 76, 340, 644, 1640
MlsI TGGCCA 1 cut(s) 356
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 3 cut(s) 451, 976, 1784
MmeI TCCRAC 4 cut(s) 658, 667, 1512, 1647
MnlI CCTC 9 cut(s) 99, 683, 707, 743, 828, 1164, 1591, 1663, 1687
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 7 cut(s) 240, 383, 501, 945, 1103, 1131, 1248
MslI CAYNNNNRTG 2 cut(s) 618, 1242
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 2 cut(s) 847, 1465
MspI CCGG 1 cut(s) 1136
MspR9I CCNGG 3 cut(s) 972, 1137, 1805
MunI CAATTG 1 cut(s) 1470
Mva1269I GAATGC 2 cut(s) 58, 806
MvaI CCWGG 2 cut(s) 972, 1805
MwoI GCNNNNNNNGC 3 cut(s) 41, 521, 1447
NciI CCSGG 1 cut(s) 1137
NdeII GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
NheI GCTAGC 1 cut(s) 50
NlaIII CATG 5 cut(s) 171, 1096, 1214, 1523, 1730
NlaIV GGNNCC 4 cut(s) 247, 1031, 1143, 1642
NmuCI GTSAC 1 cut(s) 1339
NspI RCATGY 1 cut(s) 1730
OliI CACNNNNGTG 1 cut(s) 618
PaqCI CACCTGC 1 cut(s) 773
PctI GAATGC 2 cut(s) 58, 806
PfeI GAWTC 3 cut(s) 148, 473, 559
PflFI GACNNNGTC 1 cut(s) 1520
PflMI CCANNNNNTGG 1 cut(s) 665
PkrI GCNGC 4 cut(s) 846, 985, 1255, 1725
PleI GAGTC 3 cut(s) 450, 975, 1784
PpsI GAGTC 3 cut(s) 450, 975, 1784
Ppu21I YACGTR 1 cut(s) 1679
PshBI ATTAAT 1 cut(s) 501
PsiI TTATAA 1 cut(s) 1800
Psp6I CCWGG 2 cut(s) 970, 1803
PspGI CCWGG 2 cut(s) 970, 1803
PspN4I GGNNCC 4 cut(s) 247, 1031, 1143, 1642
PspPI GGNCC 3 cut(s) 177, 1366, 1516
PsuI RGATCY 4 cut(s) 76, 340, 644, 1640
PsyI GACNNNGTC 1 cut(s) 1520
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 4 cut(s) 1205, 1633, 1677, 1754
RsaNI GTAC 4 cut(s) 1204, 1632, 1676, 1753
RseI CAYNNNNRTG 2 cut(s) 618, 1242
SaqAI TTAA 7 cut(s) 240, 383, 501, 945, 1103, 1131, 1248
SatI GCNGC 4 cut(s) 845, 984, 1254, 1724
Sau3AI GATC 7 cut(s) 76, 340, 644, 1230, 1611, 1640, 1651
Sau96I GGNCC 3 cut(s) 177, 1366, 1516
SchI GAGTC 3 cut(s) 451, 976, 1784
ScrFI CCNGG 3 cut(s) 972, 1137, 1805
SfaNI GCATC 6 cut(s) 79, 474, 632, 970, 1543, 1798
SfcI CTRYAG 3 cut(s) 754, 852, 1557
SinI GGWCC 2 cut(s) 177, 1516
SmiMI CAYNNNNRTG 2 cut(s) 618, 1242
SmlI CTYRAG 2 cut(s) 137, 1347
SmoI CTYRAG 2 cut(s) 137, 1347
SnaBI TACGTA 1 cut(s) 1679
SsiI CCGC 4 cut(s) 459, 1253, 1465, 1708
SspI AATATT 1 cut(s) 232
SspMI CTAG 2 cut(s) 51, 881
StyD4I CCNGG 3 cut(s) 970, 1135, 1803
StyI CCWWGG 2 cut(s) 406, 1636
TaaI ACNGT 3 cut(s) 96, 596, 617
TaiI ACGT 1 cut(s) 1681
TaqI TCGA 1 cut(s) 1240
TaqII GACCGA 3 cut(s) 800, 810, 971
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 3 cut(s) 148, 473, 559
Tru1I TTAA 7 cut(s) 240, 383, 501, 945, 1103, 1131, 1248
Tru9I TTAA 7 cut(s) 240, 383, 501, 945, 1103, 1131, 1248
TscAI CASTG 4 cut(s) 99, 202, 535, 1629
TseFI GTSAC 1 cut(s) 1339
TseI GCWGC 3 cut(s) 844, 983, 1723
Tsp45I GTSAC 1 cut(s) 1339
TspDTI ATGAA 6 cut(s) 90, 102, 685, 1081, 1226, 1227
TspGWI ACGGA 1 cut(s) 1430
TspRI CASTG 4 cut(s) 99, 202, 535, 1629
Tth111I GACNNNGTC 1 cut(s) 1520
Van91I CCANNNNNTGG 1 cut(s) 665
VpaK11BI GGWCC 2 cut(s) 177, 1516
VspI ATTAAT 1 cut(s) 501
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 2 cut(s) 364, 1099
XceI RCATGY 1 cut(s) 1730
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmiI GTMKAC 2 cut(s) 742, 1391
XspI CTAG 2 cut(s) 51, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.