Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0007163

Physical Location & Seq

Genomic Coordinates Forward (+)

45339316 .. 45340673

Gene Structure 1358 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G029440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGCAATGACCACCGTGGCTTCCAGTCCATTTTTGTTATCCAAATCCCGCCCTTGCTCTTGTACTAATTACAACAAGAGGGGATTCCCACTTAGAGTCGCAGCTGTTGCTTCAATCCCATTTCAACAACCCATCAACGTGGACTACCTGGAACAAGAGTTCAGTGGCCGTGGAGTGGTATTCAAAGGCATTGGTGACGATTGCGTTGCCAAGATGAGTCTTGATAATGGCAGCAAAGCCATCTTGATGCTGCCAAGTGGCCTAATCGCGTCCTACAAAGCCTCCATGTGGCATGGTGGCACTGTTGAGTTGCTCCAATCTTCAGTCTCCCAAGAAGAAGAAGAAGAAGCCAATAGCATCCGAGGAGGGGTGTCTGTGGCTTTGGAATGTCTCAGTCTCAGTCTCGATCAAGAACAAGTTTCATGGTCTCCAAGTAATTGGGCTCTTCATGACATCACAGGAAATCCTCAGGACTCTATTCAGGTAGAACTGATCAGCACTGATTCCCATGACATGGTTCAAGTCAAGTACATTGTGACTCTCCAAGACGACGTCCTAATCTCACAGCTTATGGTCTCTAATAACTCAGATTCTTCGTCACTCCAACTAAGTGGGTGTATTTTAAGCCATCTGACAGTAAGCTCACCAGACGCTACTTACGCAGTTGGTTTGGAAAGATCAGATTTCTTTGGCAGGTTACCCATTTTGACAGGTTCAGCTATAATTCCTCCAGATTGTGGCCAGAAAAGTGAATCTCAATTCAGCCAACTATGGAAACAAATGACATTCAATGCTTGGGGTCCTAAGAATGGCCACCAAACAGAAACTGAACAAGAAGAAGAAATGGAGCAAGGGGAAGAAGATGACAATTATAAGAACTTAAGGGAGCAAATGTGTCGAATATACACCAGTGCGCCTCGAGACTTCACAATCATTGACAGGGGTAGAAGAAACTCAGTGGTGGTTGGAAGAGATGGGTTTGAAGAGCTATACATGTTCAGCCCTGGCTCAAGTCATGAATTCTACGGCGAATATGCTTATATCTGCATTGGCCAGTCGGCAGTGCTCAATCCAATAATCTTGGGGCCTAAGGAATCATGGACAGGTGGACAGCGCTTACACAATCCAAATCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009767 GO:0009773 GO:0009893 GO:0009987 GO:0010468 GO:0010604 GO:0010628 GO:0015979 GO:0016020 GO:0019222 GO:0019684 GO:0022900 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048518 GO:0050789 GO:0055114 GO:0060255 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

378

Amino Acids

41.69

Weight (kDa)

4.7

Isoelectric Point (pI)

58.41

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55370 AT1G55370
fragaria_vesca	FvH4_1g28070 FvH4_1g28070 FvH4_4g18560 FvH4_4g18560
malus_domestica	MD14G1002400.v1.1
prunus_persica	Prupe.7G003600_v2.0.a1
pyrus_communis	pycom49g00190
rosa_chinensis	RchiOBHm_Chr3g0497181
rosa_laevigata	RLG00000000831 RLG00000022473
rosa_multiflora	Rmu_sc0002276.1_g000005 Rmu_sc0002893.1_g000013 Rmu_sc0007568.1_g000002 Rmu_ssc0000474.1_g000020
rosa_roxburghii	Rroxscaffold_6G00388300
rosa_rugosa	Rorug03G0291600
rosa_samantha	Rh3AG334900 Rh3BG372600 Rh3CG369000 Rh3DG369900 Rh7CG503300 Rh7DG471700
rosa_wichuraiana	Rw3G029440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	873
AatII	GACGTC	1 cut(s)	555
Acc36I	ACCTGC	1 cut(s)	684
AccB7I	CCANNNNNTGG	2 cut(s)	514, 737
AccII	CGCG	1 cut(s)	269
AciI	CCGC	1 cut(s)	49
AcoI	YGGCCR	4 cut(s)	166, 739, 811, 1051
AcsI	RAATTY	1 cut(s)	1019
AcuI	CTGAAG	1 cut(s)	306
AcyI	GRCGYC	1 cut(s)	552
AfaI	GTAC	2 cut(s)	64, 530
AfeI	AGCGCT	1 cut(s)	1115
AfiI	CCNNNNNNNGG	6 cut(s)	175, 288, 367, 514, 737, 809
AflII	CTTAAG	1 cut(s)	880
AflIII	ACRYGT	1 cut(s)	993
AgsI	TTSAA	6 cut(s)	114, 125, 184, 521, 790, 983
AjnI	CCWGG	2 cut(s)	147, 1003
AloI	GAACNNNNNNTCC	2 cut(s)	143, 175
AluBI	AGCT	5 cut(s)	104, 568, 642, 719, 988
AluI	AGCT	5 cut(s)	104, 568, 642, 719, 988
Alw21I	GWGCWC	1 cut(s)	1068
Alw26I	GTCTC	7 cut(s)	331, 395, 401, 407, 432, 580, 915
AlwNI	CAGNNNCTG	1 cut(s)	827
Ama87I	CYCGRG	1 cut(s)	918
Aor51HI	AGCGCT	1 cut(s)	1115
AoxI	GGCC	6 cut(s)	166, 259, 739, 811, 1051, 1085
ApeKI	GCWGC	3 cut(s)	101, 231, 250
ApoI	RAATTY	1 cut(s)	1019
AspLEI	GCGC	2 cut(s)	916, 1116
AspS9I	GGNCC	2 cut(s)	800, 1085
AsuHPI	GGTGA	2 cut(s)	206, 636
AvaI	CYCGRG	1 cut(s)	918
AvaII	GGWCC	1 cut(s)	800
AxyI	CCTNAGG	2 cut(s)	468, 1089
BalI	TGGCCA	3 cut(s)	741, 813, 1053
BanII	GRGCYC	1 cut(s)	445
Bbv12I	GWGCWC	1 cut(s)	1068
BbvI	GCAGC	3 cut(s)	113, 237, 243
BccI	CCATC	4 cut(s)	140, 248, 636, 968
BceAI	ACGGC	2 cut(s)	153, 1042
BcgI	CGANNNNNNTGC	4 cut(s)	188, 222, 878, 912
BciT130I	CCWGG	2 cut(s)	149, 1005
BclI	TGATCA	1 cut(s)	492
BcoDI	GTCTC	7 cut(s)	331, 395, 401, 407, 432, 580, 915
BfoI	RGCGCY	1 cut(s)	1117
BfrI	CTTAAG	1 cut(s)	880
BfuAI	ACCTGC	1 cut(s)	684
BisI	GCNGC	3 cut(s)	102, 232, 251
BlsI	GCNGC	3 cut(s)	103, 233, 252
Bme1390I	CCNGG	2 cut(s)	149, 1005
Bme18I	GGWCC	1 cut(s)	800
BmeT110I	CYCGRG	1 cut(s)	918
BmgT120I	GGNCC	2 cut(s)	800, 1085
BmiI	GGNNCC	2 cut(s)	801, 1086
BmrFI	CCNGG	2 cut(s)	149, 1005
BmsI	GCATC	2 cut(s)	237, 366
BpmI	CTGGAG	1 cut(s)	714
BpuEI	CTTGAG	1 cut(s)	994
BsaHI	GRCGYC	1 cut(s)	552
BsaI	GGTCTC	2 cut(s)	432, 580
BsaJI	CCNNGG	4 cut(s)	15, 169, 361, 1003
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 878, 908
Bsc4I	CCNNNNNNNGG	6 cut(s)	175, 288, 367, 514, 737, 809
Bse1I	ACTGG	3 cut(s)	24, 909, 1054
Bse21I	CCTNAGG	2 cut(s)	468, 1089
Bse3DI	GCAATG	1 cut(s)	12
BseBI	CCWGG	2 cut(s)	149, 1005
BseDI	CCNNGG	4 cut(s)	15, 169, 361, 1003
BseGI	GGATG	1 cut(s)	357
BseLI	CCNNNNNNNGG	6 cut(s)	175, 288, 367, 514, 737, 809
BseMI	GCAATG	1 cut(s)	12
BseMII	CTCAG	5 cut(s)	406, 412, 482, 600, 969
BseNI	ACTGG	3 cut(s)	24, 909, 1054
BseRI	GAGGAG	1 cut(s)	378
BseXI	GCAGC	3 cut(s)	113, 237, 243
Bsh1236I	CGCG	1 cut(s)	269
BshFI	GGCC	6 cut(s)	168, 261, 741, 813, 1053, 1087
BsiHKAI	GWGCWC	1 cut(s)	1068
BsiHKCI	CYCGRG	1 cut(s)	918
BslI	CCNNNNNNNGG	6 cut(s)	175, 288, 367, 514, 737, 809
BsmAI	GTCTC	7 cut(s)	331, 395, 401, 407, 432, 580, 915
BsnI	GGCC	6 cut(s)	168, 261, 741, 813, 1053, 1087
Bso31I	GGTCTC	2 cut(s)	432, 580
BsoBI	CYCGRG	1 cut(s)	918
Bsp1286I	GDGCHC	2 cut(s)	445, 1068
Bsp143I	GATC	3 cut(s)	406, 492, 677
BspACI	CCGC	1 cut(s)	49
BspANI	GGCC	6 cut(s)	168, 261, 741, 813, 1053, 1087
BspCNI	CTCAG	5 cut(s)	405, 411, 481, 599, 968
BspFNI	CGCG	1 cut(s)	269
BspHI	TCATGA	2 cut(s)	448, 1015
BspLI	GGNNCC	2 cut(s)	801, 1086
BspMI	ACCTGC	1 cut(s)	684
BspQI	GCTCTTC	2 cut(s)	450, 978
BspTI	CTTAAG	1 cut(s)	880
BspTNI	GGTCTC	2 cut(s)	432, 580
BsrDI	GCAATG	1 cut(s)	12
BsrI	ACTGG	3 cut(s)	24, 909, 1054
BssECI	CCNNGG	4 cut(s)	15, 169, 361, 1003
BssMI	GATC	3 cut(s)	406, 492, 677
BssNI	GRCGYC	1 cut(s)	552
Bst2UI	CCWGG	2 cut(s)	149, 1005
Bst4CI	ACNGT	3 cut(s)	16, 304, 637
Bst6I	CTCTTC	3 cut(s)	450, 964, 978
BstACI	GRCGYC	1 cut(s)	552
BstAFI	CTTAAG	1 cut(s)	880
BstAPI	GCANNNNNTGC	1 cut(s)	107
BstDEI	CTNAG	9 cut(s)	92, 392, 398, 468, 586, 608, 804, 955, 1089
BstDSI	CCRYGG	2 cut(s)	15, 169
BstEII	GGTNACC	1 cut(s)	696
BstF5I	GGATG	1 cut(s)	357
BstFNI	CGCG	1 cut(s)	269
BstH2I	RGCGCY	1 cut(s)	1117
BstHHI	GCGC	2 cut(s)	916, 1116
BstKTI	GATC	3 cut(s)	409, 495, 680
BstMAI	GTCTC	7 cut(s)	331, 395, 401, 407, 432, 580, 915
BstMBI	GATC	3 cut(s)	406, 492, 677
BstMWI	GCNNNNNNNGC	2 cut(s)	107, 659
BstNI	CCWGG	2 cut(s)	149, 1005
BstNSI	RCATGY	1 cut(s)	997
BstPI	GGTNACC	1 cut(s)	696
BstSCI	CCNGG	2 cut(s)	147, 1003
BstUI	CGCG	1 cut(s)	269
BstV1I	GCAGC	3 cut(s)	113, 237, 243
BstXI	CCANNNNNNTGG	3 cut(s)	139, 438, 611
Bsu36I	CCTNAGG	2 cut(s)	468, 1089
BsuRI	GGCC	6 cut(s)	168, 261, 741, 813, 1053, 1087
BtgI	CCRYGG	2 cut(s)	15, 169
BtsCI	GGATG	1 cut(s)	357
BtsI	GCAGTG	1 cut(s)	1068
BtsIMutI	CAGTG	6 cut(s)	169, 300, 498, 916, 963, 1068
BveI	ACCTGC	1 cut(s)	684
CaiI	CAGNNNCTG	1 cut(s)	827
CciI	TCATGA	2 cut(s)	448, 1015
CfoI	GCGC	2 cut(s)	916, 1116
Cfr13I	GGNCC	2 cut(s)	800, 1085
CseI	GACGC	2 cut(s)	258, 659
Csp6I	GTAC	2 cut(s)	63, 529
CviAII	CATG	9 cut(s)	286, 293, 423, 449, 509, 514, 994, 1016, 1098
CviQI	GTAC	2 cut(s)	63, 529
DdeI	CTNAG	9 cut(s)	92, 392, 398, 468, 586, 608, 804, 955, 1089
DpnI	GATC	3 cut(s)	408, 494, 679
DpnII	GATC	3 cut(s)	406, 492, 677
EaeI	YGGCCR	4 cut(s)	166, 739, 811, 1051
Eam1104I	CTCTTC	3 cut(s)	450, 964, 978
EarI	CTCTTC	3 cut(s)	450, 964, 978
Eco24I	GRGCYC	1 cut(s)	445
Eco31I	GGTCTC	2 cut(s)	432, 580
Eco47I	GGWCC	1 cut(s)	800
Eco47III	AGCGCT	1 cut(s)	1115
Eco57I	CTGAAG	1 cut(s)	306
Eco81I	CCTNAGG	2 cut(s)	468, 1089
Eco88I	CYCGRG	1 cut(s)	918
Eco91I	GGTNACC	1 cut(s)	696
EcoO109I	RGGNCCY	2 cut(s)	800, 1085
EcoO65I	GGTNACC	1 cut(s)	696
EcoRI	GAATTC	1 cut(s)	1019
EcoRII	CCWGG	2 cut(s)	147, 1003
EcoT38I	GRGCYC	1 cut(s)	445
FaeI	CATG	9 cut(s)	289, 296, 426, 452, 512, 517, 997, 1019, 1101
FatI	CATG	9 cut(s)	285, 292, 422, 448, 508, 513, 993, 1015, 1097
FauI	CCCGC	1 cut(s)	56
FbaI	TGATCA	1 cut(s)	492
Fnu4HI	GCNGC	3 cut(s)	102, 232, 251
FokI	GGATG	1 cut(s)	344
FriOI	GRGCYC	1 cut(s)	445
Fsp4HI	GCNGC	3 cut(s)	102, 232, 251
GlaI	GCGC	2 cut(s)	915, 1115
GluI	GCNGC	3 cut(s)	102, 232, 251
GsuI	CTGGAG	1 cut(s)	714
HaeII	RGCGCY	1 cut(s)	1117
HaeIII	GGCC	6 cut(s)	168, 261, 741, 813, 1053, 1087
HgaI	GACGC	2 cut(s)	258, 659
HhaI	GCGC	2 cut(s)	916, 1116
Hin1I	GRCGYC	1 cut(s)	552
Hin1II	CATG	9 cut(s)	289, 296, 426, 452, 512, 517, 997, 1019, 1101
Hin6I	GCGC	2 cut(s)	914, 1114
HinP1I	GCGC	2 cut(s)	914, 1114
HinfI	GANTC	9 cut(s)	84, 96, 217, 473, 503, 538, 590, 752, 1094
HphI	GGTGA	2 cut(s)	206, 636
Hpy166II	GTNNAC	2 cut(s)	142, 1109
Hpy188I	TCNGA	4 cut(s)	362, 589, 633, 682
Hpy188III	TCNNGA	9 cut(s)	221, 244, 404, 410, 449, 470, 731, 920, 1016
Hpy8I	GTNNAC	2 cut(s)	142, 1109
Hpy99I	CGWCG	1 cut(s)	554
HpyCH4III	ACNGT	3 cut(s)	16, 304, 637
HpyCH4IV	ACGT	2 cut(s)	138, 552
HpyCH4V	TGCA	1 cut(s)	1047
HpyF10VI	GCNNNNNNNGC	2 cut(s)	107, 659
HpyF3I	CTNAG	9 cut(s)	92, 392, 398, 468, 586, 608, 804, 955, 1089
HpySE526I	ACGT	2 cut(s)	138, 552
Hsp92I	GRCGYC	1 cut(s)	552
Hsp92II	CATG	9 cut(s)	289, 296, 426, 452, 512, 517, 997, 1019, 1101
HspAI	GCGC	2 cut(s)	914, 1114
Ksp22I	TGATCA	1 cut(s)	492
Kzo9I	GATC	3 cut(s)	406, 492, 677
LguI	GCTCTTC	2 cut(s)	450, 978
LmnI	GCTCC	3 cut(s)	318, 847, 886
Lsp1109I	GCAGC	3 cut(s)	113, 237, 243
LweI	GCATC	2 cut(s)	237, 366
MaeII	ACGT	2 cut(s)	138, 552
MaeIII	GTNAC	4 cut(s)	194, 535, 597, 696
MalI	GATC	3 cut(s)	408, 494, 679
MboI	GATC	3 cut(s)	406, 492, 677
MhlI	GDGCHC	2 cut(s)	445, 1068
MlsI	TGGCCA	3 cut(s)	741, 813, 1053
MluCI	AATT	6 cut(s)	67, 436, 723, 758, 868, 1019
MluNI	TGGCCA	3 cut(s)	741, 813, 1053
MlyI	GAGTC	4 cut(s)	105, 226, 467, 532
MmeI	TCCRAC	2 cut(s)	628, 946
MnlI	CCTC	7 cut(s)	72, 292, 356, 359, 477, 738, 927
Mox20I	TGGCCA	3 cut(s)	741, 813, 1053
MscI	TGGCCA	3 cut(s)	741, 813, 1053
MseI	TTAA	2 cut(s)	623, 881
MslI	CAYNNNNRTG	2 cut(s)	137, 245
Msp20I	TGGCCA	3 cut(s)	741, 813, 1053
MspA1I	CMGCKG	1 cut(s)	104
MspCI	CTTAAG	1 cut(s)	880
MspR9I	CCNGG	2 cut(s)	149, 1005
MvaI	CCWGG	2 cut(s)	149, 1005
MvnI	CGCG	1 cut(s)	269
MwoI	GCNNNNNNNGC	2 cut(s)	107, 659
NdeII	GATC	3 cut(s)	406, 492, 677
NlaIII	CATG	9 cut(s)	289, 296, 426, 452, 512, 517, 997, 1019, 1101
NlaIV	GGNNCC	2 cut(s)	801, 1086
NmuCI	GTSAC	3 cut(s)	194, 535, 597
NspI	RCATGY	1 cut(s)	997
PaeR7I	CTCGAG	1 cut(s)	918
PagI	TCATGA	2 cut(s)	448, 1015
PciI	ACATGT	1 cut(s)	993
PciSI	GCTCTTC	2 cut(s)	450, 978
PfeI	GAWTC	5 cut(s)	84, 503, 590, 752, 1094
PflFI	GACNNNGTC	1 cut(s)	551
PflMI	CCANNNNNTGG	2 cut(s)	514, 737
PkrI	GCNGC	3 cut(s)	103, 233, 252
PleI	GAGTC	4 cut(s)	104, 225, 467, 532
PpsI	GAGTC	4 cut(s)	104, 225, 467, 532
PpuMI	RGGWCCY	1 cut(s)	800
PscI	ACATGT	1 cut(s)	993
PsiI	TTATAA	1 cut(s)	873
Psp5II	RGGWCCY	1 cut(s)	800
Psp6I	CCWGG	2 cut(s)	147, 1003
PspEI	GGTNACC	1 cut(s)	696
PspGI	CCWGG	2 cut(s)	147, 1003
PspN4I	GGNNCC	2 cut(s)	801, 1086
PspPI	GGNCC	2 cut(s)	800, 1085
PspPPI	RGGWCCY	1 cut(s)	800
PstNI	CAGNNNCTG	1 cut(s)	827
PsyI	GACNNNGTC	1 cut(s)	551
PvuII	CAGCTG	1 cut(s)	104
RsaI	GTAC	2 cut(s)	64, 530
RsaNI	GTAC	2 cut(s)	63, 529
RseI	CAYNNNNRTG	2 cut(s)	137, 245
SapI	GCTCTTC	2 cut(s)	450, 978
SaqAI	TTAA	2 cut(s)	623, 881
SatI	GCNGC	3 cut(s)	102, 232, 251
Sau3AI	GATC	3 cut(s)	406, 492, 677
Sau96I	GGNCC	2 cut(s)	800, 1085
SchI	GAGTC	4 cut(s)	105, 226, 467, 532
ScrFI	CCNGG	2 cut(s)	149, 1005
SduI	GDGCHC	2 cut(s)	445, 1068
SfaNI	GCATC	2 cut(s)	237, 366
Sfr274I	CTCGAG	1 cut(s)	918
SinI	GGWCC	1 cut(s)	800
SlaI	CTCGAG	1 cut(s)	918
SmiMI	CAYNNNNRTG	2 cut(s)	137, 245
SmlI	CTYRAG	3 cut(s)	880, 918, 1009
SmoI	CTYRAG	3 cut(s)	880, 918, 1009
Sse9I	AATT	6 cut(s)	67, 436, 723, 758, 868, 1019
SsiI	CCGC	1 cut(s)	49
StyD4I	CCNGG	2 cut(s)	147, 1003
TaaI	ACNGT	3 cut(s)	16, 304, 637
TaiI	ACGT	2 cut(s)	141, 555
TaqI	TCGA	3 cut(s)	405, 898, 919
TasI	AATT	6 cut(s)	67, 436, 723, 758, 868, 1019
TatI	WGTACW	2 cut(s)	62, 528
TfiI	GAWTC	5 cut(s)	84, 503, 590, 752, 1094
Tru1I	TTAA	2 cut(s)	623, 881
Tru9I	TTAA	2 cut(s)	623, 881
TscAI	CASTG	6 cut(s)	169, 307, 505, 916, 963, 1068
TseFI	GTSAC	3 cut(s)	194, 535, 597
TseI	GCWGC	3 cut(s)	101, 231, 250
Tsp45I	GTSAC	3 cut(s)	194, 535, 597
TspDTI	ATGAA	3 cut(s)	411, 437, 1032
TspRI	CASTG	6 cut(s)	169, 307, 505, 916, 963, 1068
Tth111I	GACNNNGTC	1 cut(s)	551
Van91I	CCANNNNNTGG	2 cut(s)	514, 737
Vha464I	CTTAAG	1 cut(s)	880
VpaK11BI	GGWCC	1 cut(s)	800
XapI	RAATTY	1 cut(s)	1019
XceI	RCATGY	1 cut(s)	997
XhoI	CTCGAG	1 cut(s)	918
ZraI	GACGTC	1 cut(s)	553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.