AT1G04420

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003777
Physical Location & Seq
Forward (+)
1191519 .. 1193884
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04420.1

Sequence Viewer

Length: 1239 bp
ATGGCGTCATCTTACGTCACCTACTCAACGGTCACTCCGGTGGTCAGCAGCAGTAACATTCGAGGAGTCTTTCGTTTGGCATTCACGCGCCGCCGTGTTACTCTATCGCCGAATGCTCGGAGGAGAATATTGAGAGTTTCGGCGAAAGCGTCGACGAAGAATGCTATGGAGTATAGGAAATTAGGAGATTCCGATCTCAACATCAGCGAAGTTACTATGGGCACTATGACATTTGGGGAGCAAAATACTGAGAAAGAATCTCATGAGATGCTGAGTTATGCAATTGAAGAGGGCATCAATTGCATTGACACTGCTGAAGCTTATCCCATACCGATGAAGAAGGAGACGCAAGGTAAAACGGATCTTTATATCAGTAGCTGGTTGAAGTCTCAGCAACGTGACAAGATAGTTTTGGCAACTAAAGTATGTGGGTACTCAGAAAGGTCAGCTTACATAAGGGACAGTGGTGAGATTCTGCGTGTAGATGCTGCTAATATCAAAGAAAGTGTTGAGAAAAGTCTTAAGCGCCTTGGAACTGATTACATTGACTTGCTTCAAATACACTGGCCAGATCGATACGTACCACTCTTCGGTGATTTTTACTATGAAACGTCGAAATGGAGACCTAGTGTGCCATTCGCTGAGCAACTAAGAGCCTTTCAGGATCTCATAGTTGAAGGAAAGGTTCGCTATATCGGTGTCTCGAATGAAACTTCATACGGAGTGACGGAGTTTGTTAACACAGCAAAACTCGAAGGACTACCAAAGATTGTGAGCATCCAGAATGGTTACAGCTTGCTAGTTAGATGTCGGTATGAAGTTGATCTGGTAGAAGTATGCCACCCAAAAAATTGCAATGTTGGCTTGCTTGCTTATTCCCCTCTTGGAGGCGGCTCGTTGTCTGGGAAATACTTGGCTACAGACCAAGAAGCTACAAAAAATGCAAGGCTGAATCTTTTCCCAGGATATATGGAACGTTACAAGGGATCACTAGCCAAGGAAGCAACCATACAATACGTTGAGGTGGCGAAGAAGTACGGTTTAACTCCGGTTGAGTTAGCACTCGGGTTTGTACGTGACAGGCCCTTTGTGACGAGCACGATCATCGGGGCCACATCGGTAAAACAACTTAAAGAAGACATTGATGCTTTTCTGATGACGGAGCGGCCGTTTTCGCAGGAAGTTATGGCAGACATTGACGCCGTTTTCAAGAGGTTCAAAGACCCTTCTTTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

412

Amino Acids

46.43

Weight (kDa)

8.69

Isoelectric Point (pI)

38.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 71 - 402 8e-64 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1165
AccI GTMKAC 1 cut(s) 152
AccII CGCG 1 cut(s) 88
AciI CCGC 3 cut(s) 91, 891, 1165
AclI AACGTT 1 cut(s) 976
AclWI GGATC 3 cut(s) 369, 672, 994
AcoI YGGCCR 2 cut(s) 566, 1166
AcuI CTGAAG 1 cut(s) 336
AcyI GRCGYC 2 cut(s) 5, 1200
AfaI GTAC 4 cut(s) 434, 582, 1037, 1074
AfiI CCNNNNNNNGG 2 cut(s) 590, 887
AflII CTTAAG 1 cut(s) 521
AgsI TTSAA 6 cut(s) 287, 385, 557, 677, 1210, 1219
AjnI CCWGG 1 cut(s) 961
AleI CACNNNNGTG 1 cut(s) 38
AluBI AGCT 5 cut(s) 320, 378, 449, 795, 932
AluI AGCT 5 cut(s) 320, 378, 449, 795, 932
Alw21I GWGCWC 1 cut(s) 1100
Alw26I GTCTC 4 cut(s) 338, 393, 616, 706
AlwI GGATC 3 cut(s) 369, 672, 994
AlwNI CAGNNNCTG 1 cut(s) 378
Ama87I CYCGRG 1 cut(s) 1064
AoxI GGCC 4 cut(s) 566, 1082, 1110, 1166
ApeKI GCWGC 2 cut(s) 48, 488
ArsI GACNNNNNNTTYG 3 cut(s) 1070, 1102, 1214
Asp700I GAANNNNTTC 1 cut(s) 956
AspLEI GCGC 2 cut(s) 90, 528
AspS9I GGNCC 2 cut(s) 1083, 1110
AsuHPI GGTGA 3 cut(s) 10, 479, 605
AvaI CYCGRG 1 cut(s) 1064
BaeGI GKGCMC 1 cut(s) 224
BalI TGGCCA 1 cut(s) 568
BarI GAAGNNNNNNTAC 2 cut(s) 993, 1025
BbsI GAAGAC 1 cut(s) 1143
Bbv12I GWGCWC 1 cut(s) 1100
BbvI GCAGC 2 cut(s) 60, 475
BceAI ACGGC 3 cut(s) 78, 1153, 1187
BcgI CGANNNNNNTGC 2 cut(s) 1087, 1121
BciT130I CCWGG 1 cut(s) 963
BcoDI GTCTC 4 cut(s) 338, 393, 616, 706
BfaI CTAG 3 cut(s) 627, 800, 992
BfmI CTRYAG 1 cut(s) 918
BfoI RGCGCY 1 cut(s) 529
BfrI CTTAAG 1 cut(s) 521
BisI GCNGC 5 cut(s) 49, 91, 489, 892, 1166
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 5 cut(s) 50, 92, 490, 893, 1167
Bme1390I CCNGG 1 cut(s) 963
BmeT110I CYCGRG 1 cut(s) 1064
BmgT120I GGNCC 2 cut(s) 1083, 1110
BmiI GGNNCC 1 cut(s) 1111
BmrFI CCNGG 1 cut(s) 963
BmsI GCATC 5 cut(s) 258, 303, 475, 786, 1135
BpiI GAAGAC 1 cut(s) 1143
Bpu1102I GCTNAGC 1 cut(s) 642
Bsa29I ATCGAT 1 cut(s) 574
BsaAI YACGTR 2 cut(s) 580, 1076
BsaBI GATNNNNATC 1 cut(s) 192
BsaHI GRCGYC 2 cut(s) 5, 1200
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 3 cut(s) 529, 961, 996
BsaWI WCCGGW 2 cut(s) 37, 1048
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 613, 643, 1154, 1184
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 887
Bse1I ACTGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 862
Bse8I GATNNNNATC 1 cut(s) 192
BseBI CCWGG 1 cut(s) 963
BseCI ATCGAT 1 cut(s) 574
BseDI CCNNGG 3 cut(s) 529, 961, 996
BseGI GGATG 1 cut(s) 777
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 590, 887
BseMI GCAATG 1 cut(s) 862
BseMII CTCAG 5 cut(s) 240, 263, 404, 450, 633
BseNI ACTGG 1 cut(s) 569
BseRI GAGGAG 2 cut(s) 78, 136
BseSI GKGCMC 1 cut(s) 224
BseX3I CGGCCG 1 cut(s) 1166
BseXI GCAGC 2 cut(s) 60, 475
Bsh1236I CGCG 1 cut(s) 88
Bsh1285I CGRYCG 1 cut(s) 1169
BshFI GGCC 4 cut(s) 568, 1084, 1112, 1168
BshVI ATCGAT 1 cut(s) 574
BsiEI CGRYCG 1 cut(s) 1169
BsiHKAI GWGCWC 1 cut(s) 1100
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 2 cut(s) 38, 1049
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 2 cut(s) 590, 887
BsmAI GTCTC 4 cut(s) 338, 393, 616, 706
BsmBI CGTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 3 cut(s) 80, 118, 166
BsnI GGCC 4 cut(s) 568, 1084, 1112, 1168
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 1064
Bsp1286I GDGCHC 2 cut(s) 224, 1100
Bsp143I GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
Bsp1720I GCTNAGC 1 cut(s) 642
BspACI CCGC 3 cut(s) 91, 891, 1165
BspANI GGCC 4 cut(s) 568, 1084, 1112, 1168
BspCNI CTCAG 5 cut(s) 241, 264, 403, 449, 634
BspDI ATCGAT 1 cut(s) 574
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 1111
BspPI GGATC 3 cut(s) 369, 672, 994
BspTI CTTAAG 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 616
BsrBI CCGCTC 1 cut(s) 1165
BsrDI GCAATG 1 cut(s) 862
BsrI ACTGG 1 cut(s) 569
BssECI CCNNGG 3 cut(s) 529, 961, 996
BssMI GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
BssNI GRCGYC 2 cut(s) 5, 1200
BssT1I CCWWGG 2 cut(s) 529, 996
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 3 cut(s) 31, 464, 1040
Bst6I CTCTTC 2 cut(s) 282, 593
BstACI GRCGYC 2 cut(s) 5, 1200
BstAFI CTTAAG 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 300
BstBAI YACGTR 2 cut(s) 580, 1076
BstC8I GCNNGC 3 cut(s) 797, 866, 870
BstDEI CTNAG 6 cut(s) 249, 272, 390, 436, 642, 650
BstENI CCTNNNNNAGG 1 cut(s) 885
BstF5I GGATG 1 cut(s) 777
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 529
BstHHI GCGC 2 cut(s) 90, 528
BstKTI GATC 7 cut(s) 196, 364, 574, 667, 826, 989, 1104
BstMAI GTCTC 4 cut(s) 338, 393, 616, 706
BstMBI GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
BstMCI CGRYCG 1 cut(s) 1169
BstMWI GCNNNNNNNGC 4 cut(s) 300, 861, 1001, 1174
BstNI CCWGG 1 cut(s) 963
BstSCI CCNGG 1 cut(s) 961
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 224
BstSNI TACGTA 1 cut(s) 580
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 60, 475
BstV2I GAAGAC 1 cut(s) 1143
BstX2I RGATCY 2 cut(s) 361, 664
BstYI RGATCY 2 cut(s) 361, 664
BstZI CGGCCG 1 cut(s) 1166
Bsu15I ATCGAT 1 cut(s) 574
BsuRI GGCC 4 cut(s) 568, 1084, 1112, 1168
BsuTUI ATCGAT 1 cut(s) 574
BtsCI GGATG 1 cut(s) 777
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 309, 469, 562
Cac8I GCNNGC 3 cut(s) 797, 866, 870
CaiI CAGNNNCTG 1 cut(s) 378
CciI TCATGA 1 cut(s) 262
CfoI GCGC 2 cut(s) 90, 528
Cfr13I GGNCC 2 cut(s) 1083, 1110
ClaI ATCGAT 1 cut(s) 574
CseI GACGC 3 cut(s) 138, 355, 1208
Csp6I GTAC 4 cut(s) 433, 581, 1036, 1073
CviAII CATG 1 cut(s) 263
CviQI GTAC 4 cut(s) 433, 581, 1036, 1073
DdeI CTNAG 6 cut(s) 249, 272, 390, 436, 642, 650
DpnI GATC 7 cut(s) 195, 363, 573, 666, 825, 988, 1103
DpnII GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
EaeI YGGCCR 2 cut(s) 566, 1166
EagI CGGCCG 1 cut(s) 1166
Eam1104I CTCTTC 2 cut(s) 282, 593
EarI CTCTTC 2 cut(s) 282, 593
EclXI CGGCCG 1 cut(s) 1166
Eco105I TACGTA 1 cut(s) 580
Eco130I CCWWGG 2 cut(s) 529, 996
Eco31I GGTCTC 1 cut(s) 616
Eco52I CGGCCG 1 cut(s) 1166
Eco57I CTGAAG 1 cut(s) 336
Eco88I CYCGRG 1 cut(s) 1064
EcoNI CCTNNNNNAGG 1 cut(s) 885
EcoO109I RGGNCCY 1 cut(s) 1083
EcoRII CCWGG 1 cut(s) 961
EcoT14I CCWWGG 2 cut(s) 529, 996
ErhI CCWWGG 2 cut(s) 529, 996
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 1 cut(s) 266
FalI AAGNNNNNCTT 2 cut(s) 433, 465
FaqI GGGAC 1 cut(s) 473
FatI CATG 1 cut(s) 262
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 49, 91, 489, 892, 1166
FokI GGATG 1 cut(s) 764
Fsp4HI GCNGC 5 cut(s) 49, 91, 489, 892, 1166
FspBI CTAG 3 cut(s) 627, 800, 992
GlaI GCGC 2 cut(s) 89, 527
GluI GCNGC 5 cut(s) 49, 91, 489, 892, 1166
HaeII RGCGCY 1 cut(s) 529
HaeIII GGCC 4 cut(s) 568, 1084, 1112, 1168
HapII CCGG 2 cut(s) 38, 1049
HgaI GACGC 3 cut(s) 138, 355, 1208
HhaI GCGC 2 cut(s) 90, 528
Hin1I GRCGYC 2 cut(s) 5, 1200
Hin1II CATG 1 cut(s) 266
Hin6I GCGC 2 cut(s) 88, 526
HinP1I GCGC 2 cut(s) 88, 526
HincII GTYRAC 2 cut(s) 153, 739
HindII GTYRAC 2 cut(s) 153, 739
HindIII AAGCTT 1 cut(s) 318
HinfI GANTC 5 cut(s) 66, 188, 257, 472, 952
HpaI GTTAAC 1 cut(s) 739
HpaII CCGG 2 cut(s) 38, 1049
HphI GGTGA 3 cut(s) 10, 479, 605
Hpy166II GTNNAC 2 cut(s) 153, 739
Hpy188I TCNGA 4 cut(s) 120, 193, 439, 1155
Hpy188III TCNNGA 5 cut(s) 263, 662, 703, 781, 1210
Hpy8I GTNNAC 2 cut(s) 153, 739
Hpy99I CGWCG 3 cut(s) 154, 157, 616
HpyAV CCTTC 4 cut(s) 334, 671, 749, 1236
HpyCH4III ACNGT 3 cut(s) 31, 464, 1040
HpyCH4IV ACGT 7 cut(s) 15, 397, 579, 611, 976, 1017, 1075
HpyCH4V TGCA 4 cut(s) 281, 303, 855, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 300, 861, 1001, 1174
HpyF3I CTNAG 6 cut(s) 249, 272, 390, 436, 642, 650
HpySE526I ACGT 7 cut(s) 15, 397, 579, 611, 976, 1017, 1075
Hsp92I GRCGYC 2 cut(s) 5, 1200
Hsp92II CATG 1 cut(s) 266
HspAI GCGC 2 cut(s) 88, 526
KspAI GTTAAC 1 cut(s) 739
Kzo9I GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
LmnI GCTCC 2 cut(s) 238, 1162
Lsp1109I GCAGC 2 cut(s) 60, 475
LweI GCATC 5 cut(s) 258, 303, 475, 786, 1135
MaeI CTAG 3 cut(s) 627, 800, 992
MaeII ACGT 7 cut(s) 15, 397, 579, 611, 976, 1017, 1075
MalI GATC 7 cut(s) 195, 363, 573, 666, 825, 988, 1103
MbiI CCGCTC 1 cut(s) 1165
MboI GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
MboII GAAGA 6 cut(s) 169, 299, 349, 580, 1042, 1148
MfeI CAATTG 2 cut(s) 282, 298
MflI RGATCY 2 cut(s) 361, 664
MhlI GDGCHC 2 cut(s) 224, 1100
MlsI TGGCCA 1 cut(s) 568
MluCI AATT 4 cut(s) 179, 282, 298, 850
MluNI TGGCCA 1 cut(s) 568
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 7 cut(s) 56, 114, 283, 881, 891, 1015, 1206
Mox20I TGGCCA 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 956
MscI TGGCCA 1 cut(s) 568
MseI TTAA 4 cut(s) 522, 738, 1043, 1131
MslI CAYNNNNRTG 2 cut(s) 38, 332
Msp20I TGGCCA 1 cut(s) 568
MspCI CTTAAG 1 cut(s) 521
MspI CCGG 2 cut(s) 38, 1049
MspR9I CCNGG 1 cut(s) 963
MunI CAATTG 2 cut(s) 282, 298
Mva1269I GAATGC 3 cut(s) 80, 118, 166
MvaI CCWGG 1 cut(s) 963
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 4 cut(s) 300, 861, 1001, 1174
NdeII GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
NlaIII CATG 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 1111
NmuCI GTSAC 6 cut(s) 16, 31, 398, 724, 1076, 1090
OliI CACNNNNGTG 1 cut(s) 38
PagI TCATGA 1 cut(s) 262
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 1166
PctI GAATGC 3 cut(s) 80, 118, 166
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 4 cut(s) 188, 257, 472, 952
PkrI GCNGC 5 cut(s) 50, 92, 490, 893, 1167
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 2 cut(s) 580, 1076
Psp1406I AACGTT 1 cut(s) 976
Psp6I CCWGG 1 cut(s) 961
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 1 cut(s) 1111
PspPI GGNCC 2 cut(s) 1083, 1110
PstNI CAGNNNCTG 1 cut(s) 378
PsuI RGATCY 2 cut(s) 361, 664
RsaI GTAC 4 cut(s) 434, 582, 1037, 1074
RsaNI GTAC 4 cut(s) 433, 581, 1036, 1073
RseI CAYNNNNRTG 2 cut(s) 38, 332
SalI GTCGAC 1 cut(s) 151
SaqAI TTAA 4 cut(s) 522, 738, 1043, 1131
SatI GCNGC 5 cut(s) 49, 91, 489, 892, 1166
Sau3AI GATC 7 cut(s) 193, 361, 571, 664, 823, 986, 1101
Sau96I GGNCC 2 cut(s) 1083, 1110
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 1 cut(s) 963
SduI GDGCHC 2 cut(s) 224, 1100
SfaNI GCATC 5 cut(s) 258, 303, 475, 786, 1135
SfcI CTRYAG 1 cut(s) 918
SgrDI CGTCGACG 1 cut(s) 151
SmiMI CAYNNNNRTG 2 cut(s) 38, 332
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
SnaBI TACGTA 1 cut(s) 580
Sse9I AATT 4 cut(s) 179, 282, 298, 850
SsiI CCGC 3 cut(s) 91, 891, 1165
SspI AATATT 1 cut(s) 129
SspMI CTAG 3 cut(s) 627, 800, 992
StyD4I CCNGG 1 cut(s) 961
StyI CCWWGG 2 cut(s) 529, 996
TaaI ACNGT 3 cut(s) 31, 464, 1040
TaiI ACGT 7 cut(s) 18, 400, 582, 614, 979, 1020, 1078
TaqI TCGA 6 cut(s) 61, 152, 574, 614, 704, 753
TasI AATT 4 cut(s) 179, 282, 298, 850
TauI GCSGC 3 cut(s) 93, 894, 1168
TfiI GAWTC 4 cut(s) 188, 257, 472, 952
Tru1I TTAA 4 cut(s) 522, 738, 1043, 1131
Tru9I TTAA 4 cut(s) 522, 738, 1043, 1131
TscAI CASTG 3 cut(s) 316, 469, 569
TseFI GTSAC 6 cut(s) 16, 31, 398, 724, 1076, 1090
TseI GCWGC 2 cut(s) 48, 488
Tsp45I GTSAC 6 cut(s) 16, 31, 398, 724, 1076, 1090
TspDTI ATGAA 5 cut(s) 350, 621, 705, 723, 831
TspGWI ACGGA 4 cut(s) 374, 735, 743, 1175
TspRI CASTG 3 cut(s) 316, 469, 569
Vha464I CTTAAG 1 cut(s) 521
XagI CCTNNNNNAGG 1 cut(s) 885
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 3 cut(s) 627, 800, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.