MD16G1219600.v1.1

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003777
Physical Location & Seq
Forward (+)
21820052 .. 21825358
5307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1219600.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGCATTGGCCTCGTGCTCCACCACCTTTCTCTGCAACCACGCAGCGGCAGCGGCACCGTGGTCACATCCCAGAACTAGACCCAGAAGCTTCCGGGGGCGGAGGAGCTCCATTATCAAAGCAACCGTCACCACCGGTGCCAAGCAAAACCTGAAGACCCCATTGCAGTACAGGAAGCTCGGGGACTCGGACCTTGTAATTAGCGAAATCACTCTCGGAACCATGACATTCGGCGAGCAAAACACAGAGAAAGAAGCTCATGAGATTCTCAGCTACGCATTTGAAAATGGGATTAACGCATTGGACACTGCCGAGGCTTACCCAATTCCCATGAAGAAGGAGACACAGGGGAGCACTGATCGCTACATTGCCAGTTGGCTCAAGTCTCAACCCCGTGATAAGGTTGTTTTGGCAACAAAAGTAGCTGGCTATTCAGAGAGGTCAAGTCATTTGCGTGACAATGCCACGGTTTTGCGGGTGGATGCAGCAAACATTAGAGAAAGTGTAGAGAAAAGCCTAAAGCGCCTTGGGACAGATTACATTGATTTAATACAAATTCATTGGCCAGATCGCTATGTGCCATTATTTGGTGGGTATTCATATGATTTTTCAAAATGGAGGTCAAGTGTACCATTCGTGGAGCAACTAAAGGCTTTTCAAGAACTCATTGACGAAGGAAAGGTACGCTACATTGGTGTTTCTAATGAGACTTCATATGGAGTGATGGAATTTGTACATGCAGCTAAAGTTGAAGGGCTACCAAAGATAGTCAGTATCCAAAACAATTACAGTCTTCTAGTTAGAACTCATTTTGAAATTGACCTTGTTGAAGTCTGCCACCCACAAAATTACAACATTGGCTTGTTGGCTTATTCTCCACTGGCTGGTGGATCACTGAGTGGGAAGTACATAGATAGCACTTCCGAAGCTTCAAAAAAAGGGAGGCTAAACCTCTTCCCCGGCTACATGGAAAGATATAACAAATCTTCTGCTAGGGAAGCAACAATAAAGTACATCGAGCTAGGCAAGAAGTACGGGCTAACTCCAGTTCAGCTTGCACTTGGATTTGTACGGGACCGTCCATTTGTGACAAGTTCAATCGTTGGTGCAACTTCTGTCACCCAACTGAAGGAAGATGTTGATGCTTTTCTGTTGACTGAGAGGCCTTTACCACAAGAAGTGATGGCTGATATTGAAGAGATTTTCAAGAGATACAAAGACCCTACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

411

Amino Acids

45.96

Weight (kDa)

8.81

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 69 - 401 5.3e-68 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 55, 137
AccB7I CCANNNNNTGG 2 cut(s) 587, 884
AciI CCGC 4 cut(s) 47, 53, 100, 475
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 2 cut(s) 555, 728
AcuI CTGAAG 2 cut(s) 173, 1148
AdeI CACNNNGTG 1 cut(s) 899
AfaI GTAC 8 cut(s) 170, 630, 684, 735, 908, 1013, 1034, 1071
AfiI CCNNNNNNNGG 5 cut(s) 46, 400, 587, 884, 1129
AgeI ACCGGT 1 cut(s) 134
Alw21I GWGCWC 3 cut(s) 20, 110, 356
Alw26I GTCTC 3 cut(s) 335, 390, 701
AlwI GGATC 1 cut(s) 898
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 3 cut(s) 9, 563, 1163
ApeKI GCWGC 4 cut(s) 44, 50, 485, 740
ApoI RAATTY 2 cut(s) 555, 728
ArsI GACNNNNNNTTYG 6 cut(s) 111, 143, 605, 637, 1067, 1099
AsiGI ACCGGT 1 cut(s) 134
AspLEI GCGC 1 cut(s) 525
AspS9I GGNCC 2 cut(s) 190, 1075
AsuC2I CCSGG 2 cut(s) 95, 960
AsuHPI GGTGA 2 cut(s) 121, 1111
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 2 cut(s) 190, 1075
BalI TGGCCA 1 cut(s) 565
BanI GGYRCC 2 cut(s) 55, 137
BanII GRGCYC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 2 cut(s) 161, 785
Bbv12I GWGCWC 3 cut(s) 20, 110, 356
BbvI GCAGC 4 cut(s) 56, 62, 497, 752
BccI CCATC 2 cut(s) 718, 1177
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 785
BcnI CCSGG 2 cut(s) 95, 960
BcoDI GTCTC 3 cut(s) 335, 390, 701
BfaI CTAG 4 cut(s) 78, 797, 993, 1022
BfoI RGCGCY 1 cut(s) 526
BfuI GTATCC 1 cut(s) 785
BisI GCNGC 6 cut(s) 45, 48, 51, 54, 486, 741
BlsI GCNGC 6 cut(s) 46, 49, 52, 55, 487, 742
Bme1390I CCNGG 2 cut(s) 95, 960
Bme18I GGWCC 2 cut(s) 190, 1075
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 2 cut(s) 190, 1075
BmiI GGNNCC 4 cut(s) 57, 139, 220, 1076
BmrFI CCNGG 2 cut(s) 95, 960
BmsI GCATC 2 cut(s) 472, 1132
BpiI GAAGAC 2 cut(s) 161, 785
BpmI CTGGAG 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 95, 960
BsaJI CCNNGG 6 cut(s) 59, 94, 312, 465, 526, 958
BsaWI WCCGGW 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 400, 587, 884, 1129
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 3 cut(s) 372, 885, 1046
Bse3DI GCAATG 2 cut(s) 161, 366
BseDI CCNNGG 6 cut(s) 59, 94, 312, 465, 526, 958
BseGI GGATG 2 cut(s) 67, 487
BseLI CCNNNNNNNGG 5 cut(s) 46, 400, 587, 884, 1129
BseMI GCAATG 2 cut(s) 161, 366
BseMII CTCAG 3 cut(s) 283, 887, 1149
BseNI ACTGG 3 cut(s) 372, 885, 1046
BseRI GAGGAG 1 cut(s) 118
BseXI GCAGC 4 cut(s) 56, 62, 497, 752
BshFI GGCC 3 cut(s) 11, 565, 1165
BshNI GGYRCC 2 cut(s) 55, 137
BshTI ACCGGT 1 cut(s) 134
BsiHKAI GWGCWC 3 cut(s) 20, 110, 356
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 3 cut(s) 94, 135, 960
BslFI GGGAC 3 cut(s) 197, 544, 1088
BslI CCNNNNNNNGG 5 cut(s) 46, 400, 587, 884, 1129
BsmAI GTCTC 3 cut(s) 335, 390, 701
BsmFI GGGAC 3 cut(s) 197, 544, 1088
BsnI GGCC 3 cut(s) 11, 565, 1165
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 3 cut(s) 20, 110, 356
Bsp1407I TGTACA 1 cut(s) 733
Bsp143I GATC 3 cut(s) 358, 568, 890
BspACI CCGC 4 cut(s) 47, 53, 100, 475
BspANI GGCC 3 cut(s) 11, 565, 1165
BspCNI CTCAG 3 cut(s) 282, 888, 1150
BspHI TCATGA 1 cut(s) 259
BspLI GGNNCC 4 cut(s) 57, 139, 220, 1076
BspPI GGATC 1 cut(s) 898
BspT107I GGYRCC 2 cut(s) 55, 137
BsrDI GCAATG 2 cut(s) 161, 366
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 733
BsrI ACTGG 3 cut(s) 372, 885, 1046
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 6 cut(s) 59, 94, 312, 465, 526, 958
BssMI GATC 3 cut(s) 358, 568, 890
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 526
Bst2BI CACGAG 1 cut(s) 13
Bst4CI ACNGT 5 cut(s) 60, 127, 469, 791, 1079
Bst6I CTCTTC 2 cut(s) 959, 1191
BstAUI TGTACA 1 cut(s) 733
BstC8I GCNNGC 3 cut(s) 236, 427, 1056
BstDEI CTNAG 3 cut(s) 269, 896, 1158
BstDSI CCRYGG 2 cut(s) 59, 465
BstF5I GGATG 2 cut(s) 67, 487
BstH2I RGCGCY 1 cut(s) 526
BstHHI GCGC 1 cut(s) 525
BstKTI GATC 3 cut(s) 361, 571, 893
BstMAI GTCTC 3 cut(s) 335, 390, 701
BstMBI GATC 3 cut(s) 358, 568, 890
BstMWI GCNNNNNNNGC 5 cut(s) 50, 53, 360, 522, 998
BstNSI RCATGY 1 cut(s) 740
BstSCI CCNGG 2 cut(s) 93, 958
BstV1I GCAGC 4 cut(s) 56, 62, 497, 752
BstV2I GAAGAC 2 cut(s) 161, 785
BsuI GTATCC 1 cut(s) 785
BsuRI GGCC 3 cut(s) 11, 565, 1165
BtgI CCRYGG 2 cut(s) 59, 465
BtsCI GGATG 2 cut(s) 67, 487
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 4 cut(s) 306, 354, 878, 893
Cac8I GCNNGC 3 cut(s) 236, 427, 1056
CciI TCATGA 1 cut(s) 259
CfoI GCGC 1 cut(s) 525
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 2 cut(s) 190, 1075
Csp6I GTAC 8 cut(s) 169, 629, 683, 734, 907, 1012, 1033, 1070
CspAI ACCGGT 1 cut(s) 134
CviAII CATG 5 cut(s) 223, 260, 331, 737, 967
CviQI GTAC 8 cut(s) 169, 629, 683, 734, 907, 1012, 1033, 1070
DdeI CTNAG 3 cut(s) 269, 896, 1158
DpnI GATC 3 cut(s) 360, 570, 892
DpnII GATC 3 cut(s) 358, 568, 890
DraIII CACNNNGTG 1 cut(s) 899
EaeI YGGCCR 1 cut(s) 563
Eam1104I CTCTTC 2 cut(s) 959, 1191
EarI CTCTTC 2 cut(s) 959, 1191
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 526
Eco147I AGGCCT 1 cut(s) 1165
Eco24I GRGCYC 1 cut(s) 110
Eco47I GGWCC 2 cut(s) 190, 1075
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 2 cut(s) 173, 1148
Eco88I CYCGRG 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 526
FaeI CATG 5 cut(s) 226, 263, 334, 740, 970
FaqI GGGAC 3 cut(s) 197, 544, 1088
FatI CATG 5 cut(s) 222, 259, 330, 736, 966
FauI CCCGC 1 cut(s) 468
FauNDI CATATG 2 cut(s) 601, 715
Fnu4HI GCNGC 6 cut(s) 45, 48, 51, 54, 486, 741
FokI GGATG 2 cut(s) 54, 494
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 6 cut(s) 45, 48, 51, 54, 486, 741
FspBI CTAG 4 cut(s) 78, 797, 993, 1022
GlaI GCGC 1 cut(s) 524
GluI GCNGC 6 cut(s) 45, 48, 51, 54, 486, 741
GsuI CTGGAG 1 cut(s) 1029
HaeII RGCGCY 1 cut(s) 526
HaeIII GGCC 3 cut(s) 11, 565, 1165
HapII CCGG 3 cut(s) 94, 135, 960
HhaI GCGC 1 cut(s) 525
Hin1II CATG 5 cut(s) 226, 263, 334, 740, 970
Hin6I GCGC 1 cut(s) 523
HinP1I GCGC 1 cut(s) 523
HincII GTYRAC 1 cut(s) 1155
HindII GTYRAC 1 cut(s) 1155
HindIII AAGCTT 2 cut(s) 88, 927
HinfI GANTC 2 cut(s) 185, 265
HpaII CCGG 3 cut(s) 94, 135, 960
HphI GGTGA 2 cut(s) 121, 1111
Hpy166II GTNNAC 2 cut(s) 629, 1155
Hpy188I TCNGA 4 cut(s) 190, 218, 436, 925
Hpy188III TCNNGA 3 cut(s) 260, 659, 1207
Hpy8I GTNNAC 2 cut(s) 629, 1155
HpyAV CCTTC 4 cut(s) 331, 668, 746, 1123
HpyCH4III ACNGT 5 cut(s) 60, 127, 469, 791, 1079
HpyCH4V TGCA 6 cut(s) 36, 166, 485, 740, 1058, 1109
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 53, 360, 522, 998
HpyF3I CTNAG 3 cut(s) 269, 896, 1158
Hsp92II CATG 5 cut(s) 226, 263, 334, 740, 970
HspAI GCGC 1 cut(s) 523
Kzo9I GATC 3 cut(s) 358, 568, 890
LmnI GCTCC 5 cut(s) 23, 105, 113, 351, 640
Lsp1109I GCAGC 4 cut(s) 56, 62, 497, 752
LweI GCATC 2 cut(s) 472, 1132
MaeI CTAG 4 cut(s) 78, 797, 993, 1022
MaeIII GTNAC 5 cut(s) 63, 127, 455, 1087, 1117
MalI GATC 3 cut(s) 360, 570, 892
MboI GATC 3 cut(s) 358, 568, 890
MboII GAAGA 7 cut(s) 166, 346, 785, 946, 978, 1145, 1208
MhlI GDGCHC 3 cut(s) 20, 110, 356
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 7 cut(s) 198, 324, 555, 728, 784, 816, 847
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 8 cut(s) 22, 96, 307, 432, 612, 936, 962, 1155
Mox20I TGGCCA 1 cut(s) 565
MscI TGGCCA 1 cut(s) 565
MseI TTAA 2 cut(s) 294, 548
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 565
MspA1I CMGCKG 2 cut(s) 47, 53
MspI CCGG 3 cut(s) 94, 135, 960
MspR9I CCNGG 2 cut(s) 95, 960
MwoI GCNNNNNNNGC 5 cut(s) 50, 53, 360, 522, 998
NciI CCSGG 2 cut(s) 95, 960
NdeI CATATG 2 cut(s) 601, 715
NdeII GATC 3 cut(s) 358, 568, 890
NlaIII CATG 5 cut(s) 226, 263, 334, 740, 970
NlaIV GGNNCC 4 cut(s) 57, 139, 220, 1076
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 5 cut(s) 63, 127, 455, 1087, 1117
NspI RCATGY 1 cut(s) 740
PagI TCATGA 1 cut(s) 259
PceI AGGCCT 1 cut(s) 1165
PfeI GAWTC 1 cut(s) 265
PflMI CCANNNNNTGG 2 cut(s) 587, 884
PinAI ACCGGT 1 cut(s) 134
PkrI GCNGC 6 cut(s) 46, 49, 52, 55, 487, 742
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 110
PspN4I GGNNCC 4 cut(s) 57, 139, 220, 1076
PspPI GGNCC 2 cut(s) 190, 1075
RsaI GTAC 8 cut(s) 170, 630, 684, 735, 908, 1013, 1034, 1071
RsaNI GTAC 8 cut(s) 169, 629, 683, 734, 907, 1012, 1033, 1070
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 2 cut(s) 294, 548
SatI GCNGC 6 cut(s) 45, 48, 51, 54, 486, 741
Sau3AI GATC 3 cut(s) 358, 568, 890
Sau96I GGNCC 2 cut(s) 190, 1075
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 95, 960
SduI GDGCHC 3 cut(s) 20, 110, 356
SfaNI GCATC 2 cut(s) 472, 1132
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 2 cut(s) 190, 1075
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 7 cut(s) 198, 324, 555, 728, 784, 816, 847
SseBI AGGCCT 1 cut(s) 1165
SsiI CCGC 4 cut(s) 47, 53, 100, 475
SspMI CTAG 4 cut(s) 78, 797, 993, 1022
SstI GAGCTC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 1165
StyD4I CCNGG 2 cut(s) 93, 958
StyI CCWWGG 1 cut(s) 526
TaaI ACNGT 5 cut(s) 60, 127, 469, 791, 1079
TaqI TCGA 1 cut(s) 1017
TasI AATT 7 cut(s) 198, 324, 555, 728, 784, 816, 847
TatI WGTACW 4 cut(s) 168, 733, 906, 1011
TauI GCSGC 2 cut(s) 50, 56
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 2 cut(s) 294, 548
Tru9I TTAA 2 cut(s) 294, 548
TscAI CASTG 4 cut(s) 313, 361, 885, 900
TseFI GTSAC 5 cut(s) 63, 127, 455, 1087, 1117
TseI GCWGC 4 cut(s) 44, 50, 485, 740
Tsp45I GTSAC 5 cut(s) 63, 127, 455, 1087, 1117
TspDTI ATGAA 4 cut(s) 347, 548, 588, 702
TspRI CASTG 4 cut(s) 313, 361, 885, 900
Van91I CCANNNNNTGG 2 cut(s) 587, 884
VpaK11BI GGWCC 2 cut(s) 190, 1075
XapI RAATTY 2 cut(s) 555, 728
XceI RCATGY 1 cut(s) 740
XspI CTAG 4 cut(s) 78, 797, 993, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.