AT1G14890

enzyme inhibitor pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004993
Physical Location & Seq
Forward (+)
5136874 .. 5137982
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14890.1

Sequence Viewer

Length: 660 bp
ATGTTAACTCGAAACAAAGAAGAAATAAACAGAGTAAAAAACTTAAAGCTTAAAATGGGGAGGCAGCTTTATACGACGACGGTTCTCTACTTAGTCACCCTACTCTTCATTTGCCGGACAATCTCAGCCGTCCGTTTCCCTCCGGAACAACCAACAACCGATGATCTAGATTTCATCCGTACGAGCTGCAACACAACGCTGTATCCAGACGTATGTTACACGTCTCTAGCTGGATACGCCTCCGCCGTCCAAGATAATCCGGCAAGGCTAGCTAAACTCGCAATCGGCGTTTCTCTCTCCCGCGCAAAATACACGGCGGCTTACCTCTCCAAACTCTCACGTCGCGCTGCCTCAGCTGCTGTCCACGACTGCGTTTCGAACGTGGGAGATGCCGTGGATCAGATGCGTGGCTCCCTAAGGCAGCTCCGAGAGATGAACCACCGTCGTCCCGGAGATCCGGCGTTTAGGTTTCAGATGAGTAACGTGCAGACGTGGATGAGCGCGGCGTTGACGGACGAGGAGACGTGTACGGATGGGGTCACGGAGGAGATGGAAGACGGAGAGACGAAGACGGCTATTTGCGATAGAGTCGCCGACGTCAAGAGGTTCACGAGTAATGCGCTCGCTCTCGTTAATACCTACGCCAACAATGGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

219

Amino Acids

24.32

Weight (kDa)

8.52

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 57 - 210 2.7e-38 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 600
AccII CGCG 3 cut(s) 303, 345, 503
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 4 cut(s) 243, 301, 317, 503
AclWI GGATC 2 cut(s) 405, 449
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 2 cut(s) 181, 529
AflIII ACRYGT 2 cut(s) 219, 524
AjiI CACGTC 4 cut(s) 222, 341, 492, 525
AluBI AGCT 7 cut(s) 49, 67, 186, 230, 272, 356, 424
AluI AGCT 7 cut(s) 49, 67, 186, 230, 272, 356, 424
Alw26I GTCTC 3 cut(s) 228, 515, 557
AlwI GGATC 2 cut(s) 405, 449
AlwNI CAGNNNCTG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 142
ApeKI GCWGC 5 cut(s) 64, 186, 347, 356, 421
AspLEI GCGC 4 cut(s) 305, 347, 503, 622
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 377
AsuNHI GCTAGC 1 cut(s) 268
AxyI CCTNAGG 1 cut(s) 416
BauI CACGAG 1 cut(s) 610
BbsI GAAGAC 2 cut(s) 561, 575
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 5 cut(s) 76, 173, 334, 343, 433
BccI CCATC 2 cut(s) 527, 544
BceAI ACGGC 5 cut(s) 113, 230, 330, 377, 588
BciVI GTATCC 2 cut(s) 213, 227
BcnI CCSGG 1 cut(s) 450
BcoDI GTCTC 3 cut(s) 228, 515, 557
BfaI CTAG 3 cut(s) 167, 227, 269
BfuI GTATCC 2 cut(s) 213, 227
BisI GCNGC 7 cut(s) 65, 187, 318, 348, 357, 422, 504
BlsI GCNGC 7 cut(s) 66, 188, 319, 349, 358, 423, 505
Bme1390I CCNGG 1 cut(s) 450
BmgBI CACGTC 4 cut(s) 222, 341, 492, 525
BmiI GGNNCC 2 cut(s) 412, 655
BmrFI CCNGG 1 cut(s) 450
BmsI GCATC 2 cut(s) 379, 393
BmtI GCTAGC 1 cut(s) 272
BpiI GAAGAC 2 cut(s) 561, 575
Bpu10I CCTNAGC 1 cut(s) 352
Bpu14I TTCGAA 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 597
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bse21I CCTNAGG 1 cut(s) 416
BseAI TCCGGA 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 3 cut(s) 174, 501, 538
BseMII CTCAG 2 cut(s) 138, 366
BseRI GAGGAG 2 cut(s) 533, 560
BseXI GCAGC 5 cut(s) 76, 173, 334, 343, 433
BsgI GTGCAG 1 cut(s) 506
Bsh1236I CGCG 3 cut(s) 303, 345, 503
BsiSI CCGG 5 cut(s) 115, 143, 260, 450, 458
BsiWI CGTACG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 432
BsmAI GTCTC 3 cut(s) 228, 515, 557
BsmBI CGTCTC 3 cut(s) 228, 515, 557
BsmFI GGGAC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 377
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 3 cut(s) 163, 397, 454
BspACI CCGC 4 cut(s) 243, 301, 317, 503
BspCNI CTCAG 2 cut(s) 137, 365
BspEI TCCGGA 1 cut(s) 142
BspFNI CGCG 3 cut(s) 303, 345, 503
BspLI GGNNCC 2 cut(s) 412, 655
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 2 cut(s) 405, 449
BspT104I TTCGAA 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 393
BssMI GATC 3 cut(s) 163, 397, 454
BssNI GRCGYC 1 cut(s) 597
BssSI CACGAG 1 cut(s) 610
Bst2BI CACGAG 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 82, 443
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 597
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 270, 624
BstDEI CTNAG 4 cut(s) 91, 124, 352, 416
BstDSI CCRYGG 1 cut(s) 393
BstF5I GGATG 3 cut(s) 174, 501, 538
BstFNI CGCG 3 cut(s) 303, 345, 503
BstHHI GCGC 4 cut(s) 305, 347, 503, 622
BstKTI GATC 3 cut(s) 166, 400, 457
BstMAI GTCTC 3 cut(s) 228, 515, 557
BstMBI GATC 3 cut(s) 163, 397, 454
BstMWI GCNNNNNNNGC 5 cut(s) 236, 269, 278, 353, 356
BstSCI CCNGG 1 cut(s) 448
BstUI CGCG 3 cut(s) 303, 345, 503
BstV1I GCAGC 5 cut(s) 76, 173, 334, 343, 433
BstV2I GAAGAC 2 cut(s) 561, 575
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
Bsu36I CCTNAGG 1 cut(s) 416
BsuI GTATCC 2 cut(s) 213, 227
BtgI CCRYGG 1 cut(s) 393
BtrI CACGTC 4 cut(s) 222, 341, 492, 525
BtsCI GGATG 3 cut(s) 174, 501, 538
Cac8I GCNNGC 2 cut(s) 270, 624
CaiI CAGNNNCTG 1 cut(s) 359
CfoI GCGC 4 cut(s) 305, 347, 503, 622
Csp6I GTAC 2 cut(s) 180, 528
CviQI GTAC 2 cut(s) 180, 528
DdeI CTNAG 4 cut(s) 91, 124, 352, 416
DpnI GATC 3 cut(s) 165, 399, 456
DpnII GATC 3 cut(s) 163, 397, 454
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 1 cut(s) 232
Eco81I CCTNAGG 1 cut(s) 416
Esp3I CGTCTC 3 cut(s) 228, 515, 557
FaiI YATR 2 cut(s) 72, 214
FaqI GGGAC 1 cut(s) 432
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 7 cut(s) 65, 187, 318, 348, 357, 422, 504
FokI GGATG 3 cut(s) 161, 508, 545
Fsp4HI GCNGC 7 cut(s) 65, 187, 318, 348, 357, 422, 504
FspBI CTAG 3 cut(s) 167, 227, 269
GlaI GCGC 4 cut(s) 304, 346, 502, 621
GluI GCNGC 7 cut(s) 65, 187, 318, 348, 357, 422, 504
HapII CCGG 5 cut(s) 115, 143, 260, 450, 458
HhaI GCGC 4 cut(s) 305, 347, 503, 622
Hin1I GRCGYC 1 cut(s) 597
Hin6I GCGC 4 cut(s) 303, 345, 501, 620
HinP1I GCGC 4 cut(s) 303, 345, 501, 620
HincII GTYRAC 2 cut(s) 6, 510
HindII GTYRAC 2 cut(s) 6, 510
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 588
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 5 cut(s) 115, 143, 260, 450, 458
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 5 cut(s) 6, 364, 510, 528, 609
Hpy188I TCNGA 3 cut(s) 402, 428, 474
Hpy188III TCNNGA 5 cut(s) 143, 167, 206, 601, 610
Hpy8I GTNNAC 5 cut(s) 6, 364, 510, 528, 609
Hpy99I CGWCG 5 cut(s) 79, 82, 345, 447, 599
HpyCH4III ACNGT 2 cut(s) 82, 443
HpyCH4IV ACGT 8 cut(s) 210, 221, 340, 381, 483, 491, 524, 597
HpyCH4V TGCA 2 cut(s) 189, 487
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 269, 278, 353, 356
HpyF3I CTNAG 4 cut(s) 91, 124, 352, 416
HpySE526I ACGT 8 cut(s) 210, 221, 340, 381, 483, 491, 524, 597
Hsp92I GRCGYC 1 cut(s) 597
HspAI GCGC 4 cut(s) 303, 345, 501, 620
Kpn2I TCCGGA 1 cut(s) 142
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 163, 397, 454
LmnI GCTCC 3 cut(s) 416, 429, 653
LpnPI CCDG 7 cut(s) 128, 156, 216, 219, 273, 463, 471
Lsp1109I GCAGC 5 cut(s) 76, 173, 334, 343, 433
LweI GCATC 2 cut(s) 379, 393
MaeI CTAG 3 cut(s) 167, 227, 269
MaeII ACGT 8 cut(s) 210, 221, 340, 381, 483, 491, 524, 597
MaeIII GTNAC 4 cut(s) 94, 215, 479, 538
MalI GATC 3 cut(s) 165, 399, 456
MboI GATC 3 cut(s) 163, 397, 454
MboII GAAGA 4 cut(s) 32, 97, 566, 580
MflI RGATCY 1 cut(s) 454
MlyI GAGTC 1 cut(s) 597
MnlI CCTC 8 cut(s) 54, 150, 250, 335, 361, 511, 538, 597
MroI TCCGGA 1 cut(s) 142
MseI TTAA 4 cut(s) 5, 44, 51, 633
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 5 cut(s) 115, 143, 260, 450, 458
MspR9I CCNGG 1 cut(s) 450
MvnI CGCG 3 cut(s) 303, 345, 503
MwoI GCNNNNNNNGC 5 cut(s) 236, 269, 278, 353, 356
NciI CCSGG 1 cut(s) 450
NdeII GATC 3 cut(s) 163, 397, 454
NheI GCTAGC 1 cut(s) 268
NlaIV GGNNCC 2 cut(s) 412, 655
NmuCI GTSAC 2 cut(s) 94, 538
NspV TTCGAA 1 cut(s) 377
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 285
Pfl23II CGTACG 1 cut(s) 179
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 7 cut(s) 66, 188, 319, 349, 358, 423, 505
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
PspLI CGTACG 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 412, 655
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 2 cut(s) 181, 529
RsaNI GTAC 2 cut(s) 180, 528
SaqAI TTAA 4 cut(s) 5, 44, 51, 633
SatI GCNGC 7 cut(s) 65, 187, 318, 348, 357, 422, 504
Sau3AI GATC 3 cut(s) 163, 397, 454
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 1 cut(s) 450
SfaNI GCATC 2 cut(s) 379, 393
SfuI TTCGAA 1 cut(s) 377
SsiI CCGC 4 cut(s) 243, 301, 317, 503
SspMI CTAG 3 cut(s) 167, 227, 269
StyD4I CCNGG 1 cut(s) 448
TaaI ACNGT 2 cut(s) 82, 443
TaiI ACGT 8 cut(s) 213, 224, 343, 384, 486, 494, 527, 600
TaqI TCGA 2 cut(s) 10, 377
TauI GCSGC 2 cut(s) 320, 506
Tru1I TTAA 4 cut(s) 5, 44, 51, 633
Tru9I TTAA 4 cut(s) 5, 44, 51, 633
TseFI GTSAC 2 cut(s) 94, 538
TseI GCWGC 5 cut(s) 64, 186, 347, 356, 421
Tsp45I GTSAC 2 cut(s) 94, 538
TspDTI ATGAA 3 cut(s) 97, 163, 449
TspGWI ACGGA 6 cut(s) 122, 167, 527, 545, 557, 573
XbaI TCTAGA 1 cut(s) 166
XspI CTAG 3 cut(s) 167, 227, 269
ZraI GACGTC 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.