Rh4BG413600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004993
Physical Location & Seq
Forward (+)
56165599 .. 56165988
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG413600.1

Sequence Viewer

Length: 390 bp
ATGGCAGCCGTTGCCGTGAGCCTAACCAATGCAAGACGCACGGCGACTTCCCTCGCAAACCTCTCCAAACAAGCCGACCCTAAAGTCGCCGGGGCCCTCCAGGACTGCTCCAGCAACTTCGGAAAAGCCATCGACGAGATTACAGATTCACAAGAGCAGATGAAGACGTTGGGGTCGGGTACCGGGTCGGTTAGGTTTCAGCTGAGCAACGTGCAGACGTGGATGAGTGCGGCGCTTACGGAGGCGGAGACGTGTACGGATGGATTTGAGGACGTGGAGGACGGGCCGATCAAGACGGAGGTGGTGAACCAGGCTGAGATGGTGAAGAAGATGACAAGTATTGCGCTTGCGATCGTTAATGGTCTTGCTAGTAACGGCGCACCGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

129

Amino Acids

13.42

Weight (kDa)

4.59

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 118 1.1e-27 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 83, 172
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AciI CCGC 3 cut(s) 230, 245, 383
AfaI GTAC 2 cut(s) 181, 256
AflIII ACRYGT 1 cut(s) 251
AjiI CACGTC 3 cut(s) 219, 252, 274
AjnI CCWGG 2 cut(s) 99, 309
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 2 cut(s) 93, 284
ApaI GGGCCC 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 5
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 3 cut(s) 235, 346, 380
AspS9I GGNCC 3 cut(s) 93, 94, 284
AsuC2I CCSGG 2 cut(s) 91, 184
AsuHPI GGTGA 2 cut(s) 316, 334
BaeGI GKGCMC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 137, 254, 313
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 2 cut(s) 101, 311
BcnI CCSGG 2 cut(s) 91, 184
BcoDI GTCTC 1 cut(s) 242
BfaI CTAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 236
BisI GCNGC 2 cut(s) 6, 231
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 2 cut(s) 7, 232
Bme1390I CCNGG 4 cut(s) 91, 101, 184, 311
BmgBI CACGTC 3 cut(s) 219, 252, 274
BmgT120I GGNCC 3 cut(s) 93, 94, 284
BmiI GGNNCC 3 cut(s) 94, 95, 181
BmrFI CCNGG 4 cut(s) 91, 101, 184, 311
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 83, 94
Bpu1102I GCTNAGC 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 91, 184
BsaJI CCNNGG 1 cut(s) 90
BseBI CCWGG 2 cut(s) 101, 311
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 2 cut(s) 228, 265
BseMII CTCAG 2 cut(s) 194, 306
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 233
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 2 cut(s) 95, 286
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 354
BsiSI CCGG 2 cut(s) 90, 183
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsnI GGCC 2 cut(s) 95, 286
Bsp120I GGGCCC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 2 cut(s) 288, 351
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 3 cut(s) 230, 245, 383
BspANI GGCC 2 cut(s) 95, 286
BspCNI CTCAG 2 cut(s) 195, 307
BspLI GGNNCC 3 cut(s) 94, 95, 181
BspT107I GGYRCC 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 2 cut(s) 288, 351
Bst2UI CCWGG 2 cut(s) 101, 311
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 2 cut(s) 203, 315
BstF5I GGATG 2 cut(s) 228, 265
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 3 cut(s) 235, 346, 380
BstKTI GATC 2 cut(s) 291, 354
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 2 cut(s) 288, 351
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 101, 311
BstSCI CCNGG 4 cut(s) 89, 99, 182, 309
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 95, 286
BtrI CACGTC 3 cut(s) 219, 252, 274
BtsCI GGATG 2 cut(s) 228, 265
Cac8I GCNNGC 1 cut(s) 348
CfoI GCGC 3 cut(s) 235, 346, 380
Cfr13I GGNCC 3 cut(s) 93, 94, 284
CseI GACGC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 180, 255
CviJI RGCY 8 cut(s) 8, 21, 74, 95, 128, 202, 286, 314
CviKI_1 RGCY 8 cut(s) 8, 21, 74, 95, 128, 202, 286, 314
CviQI GTAC 2 cut(s) 180, 255
DdeI CTNAG 2 cut(s) 203, 315
DpnI GATC 2 cut(s) 290, 353
DpnII GATC 2 cut(s) 288, 351
DrdI GACNNNNNNGTC 2 cut(s) 83, 172
DseDI GACNNNNNNGTC 2 cut(s) 83, 172
EciI GGCGGA 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 97
EcoO109I RGGNCCY 2 cut(s) 93, 94
EcoRII CCWGG 2 cut(s) 99, 309
EcoT38I GRGCYC 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 242
Fnu4HI GCNGC 2 cut(s) 6, 231
FokI GGATG 2 cut(s) 235, 272
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 2 cut(s) 6, 231
FspBI CTAG 1 cut(s) 369
GlaI GCGC 3 cut(s) 234, 345, 379
GluI GCNGC 2 cut(s) 6, 231
GsuI CTGGAG 2 cut(s) 83, 94
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 2 cut(s) 95, 286
HapII CCGG 2 cut(s) 90, 183
HgaI GACGC 1 cut(s) 45
HhaI GCGC 3 cut(s) 235, 346, 380
Hin6I GCGC 3 cut(s) 233, 344, 378
HinP1I GCGC 3 cut(s) 233, 344, 378
HinfI GANTC 1 cut(s) 146
HpaII CCGG 2 cut(s) 90, 183
HphI GGTGA 2 cut(s) 316, 334
Hpy166II GTNNAC 2 cut(s) 255, 307
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 255, 307
Hpy99I CGWCG 1 cut(s) 137
HpyCH4IV ACGT 5 cut(s) 167, 210, 218, 251, 273
HpyCH4V TGCA 2 cut(s) 32, 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 203, 315
HpySE526I ACGT 5 cut(s) 167, 210, 218, 251, 273
HspAI GCGC 3 cut(s) 233, 344, 378
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 2 cut(s) 288, 351
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 7 cut(s) 86, 103, 113, 124, 196, 296, 323
Lsp1109I GCAGC 1 cut(s) 17
MaeI CTAG 1 cut(s) 369
MaeII ACGT 5 cut(s) 167, 210, 218, 251, 273
MaeIII GTNAC 1 cut(s) 371
MalI GATC 2 cut(s) 290, 353
MboI GATC 2 cut(s) 288, 351
MboII GAAGA 3 cut(s) 175, 337, 340
MhlI GDGCHC 1 cut(s) 97
MnlI CCTC 7 cut(s) 62, 71, 107, 235, 262, 271, 292
MseI TTAA 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 2 cut(s) 90, 183
MspR9I CCNGG 4 cut(s) 91, 101, 184, 311
MvaI CCWGG 2 cut(s) 101, 311
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 2 cut(s) 91, 184
NdeII GATC 2 cut(s) 288, 351
NlaIV GGNNCC 3 cut(s) 94, 95, 181
PfeI GAWTC 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 2 cut(s) 7, 232
Ple19I CGATCG 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 99, 309
PspGI CCWGG 2 cut(s) 99, 309
PspN4I GGNNCC 3 cut(s) 94, 95, 181
PspOMI GGGCCC 1 cut(s) 93
PspPI GGNCC 3 cut(s) 93, 94, 284
PvuI CGATCG 1 cut(s) 354
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 2 cut(s) 181, 256
RsaNI GTAC 2 cut(s) 180, 255
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 2 cut(s) 6, 231
Sau3AI GATC 2 cut(s) 288, 351
Sau96I GGNCC 3 cut(s) 93, 94, 284
ScrFI CCNGG 4 cut(s) 91, 101, 184, 311
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 63, 170, 197, 204, 213, 221, 254, 276, 303
SsiI CCGC 3 cut(s) 230, 245, 383
SspMI CTAG 1 cut(s) 369
StyD4I CCNGG 4 cut(s) 89, 99, 182, 309
TaiI ACGT 5 cut(s) 170, 213, 221, 254, 276
TaqI TCGA 1 cut(s) 132
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 146
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 176
TspGWI ACGGA 3 cut(s) 254, 272, 311
XspI CTAG 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.