AT2G01610

enzyme inhibitor pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004993
Physical Location & Seq
Reverse (-)
273895 .. 275002
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G01610.1

Sequence Viewer

Length: 669 bp
ATGGTAACCGTAAGCCAAAGTCACACGACGACGTTTCTCTTCTTCACCACATTTCTCTTGATATTCGGATCAATCTCAGCCGTCCGTTTGCTTCCACGACCAAACACTACAACAACCAACGATCTAGATTTCATCCGAACAAGCTGCAACGCTACTCTATATCCAGACGTCTGCTTCACGTCACTCTCCGGCTACGCCTCTGCCGTTCAAGACAGTCCGGCGAGGCTAGCCAAGCTCGCAATCGGCGTTTCACTTTCACAAGCCAAATCCACTGCGGCTTTTCTCTCCAAACTCTCACGCTCTGCCGCTAAATACTCCGGTGATGGCCACCAAACAGCTTCCGCCGTAATCCGAGACTGCGTTTCGAACGTCGAAGACGCGGTGGACGAGATGAGAGGATCTCTCCGTCAACTACGCGACATGAACGGCAGAGGAGGCGGCACGGCAGCTCGGAGGTCGGTAGAAACGTTTAGGTTCCAGATGAGTAACGTGCAGACGTGGATGAGTGCAGCATTGACGGATGAGGACACGTGTACGGATGGATTTGAAGATATGGACGAAGGAGGATTGATTAAGACGACCGTTTGTGATCGGCTCGAGGAAGTGAAGAGGCTAACGAGTAATGCTCTTGCCCTTGTCAACACTTACGCCAACAATGGAGCTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

222

Amino Acids

23.95

Weight (kDa)

5.71

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 43 - 212 1e-41 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 171
AccII CGCG 2 cut(s) 380, 417
AciI CCGC 5 cut(s) 275, 306, 342, 380, 438
AclI AACGTT 1 cut(s) 467
AclWI GGATC 2 cut(s) 76, 406
AcoI YGGCCR 1 cut(s) 325
AcvI CACGTG 1 cut(s) 531
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 1 cut(s) 535
AflIII ACRYGT 2 cut(s) 528, 530
AgsI TTSAA 2 cut(s) 209, 548
AjiI CACGTC 2 cut(s) 180, 498
AluBI AGCT 5 cut(s) 144, 235, 338, 449, 662
AluI AGCT 5 cut(s) 144, 235, 338, 449, 662
Alw21I GWGCWC 1 cut(s) 664
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 2 cut(s) 76, 406
Ama87I CYCGRG 1 cut(s) 596
AoxI GGCC 1 cut(s) 325
ApeKI GCWGC 3 cut(s) 144, 446, 509
AsuHPI GGTGA 2 cut(s) 37, 332
AsuII TTCGAA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 596
BalI TGGCCA 1 cut(s) 327
BanII GRGCYC 1 cut(s) 664
BbrPI CACGTG 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 664
BbvI GCAGC 3 cut(s) 131, 458, 521
BccI CCATC 2 cut(s) 317, 533
BceAI ACGGC 5 cut(s) 65, 188, 329, 442, 459
BcgI CGANNNNNNTGC 2 cut(s) 126, 160
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 2 cut(s) 125, 227
BisI GCNGC 6 cut(s) 145, 276, 306, 439, 447, 510
BlsI GCNGC 6 cut(s) 146, 277, 307, 440, 448, 511
BmeT110I CYCGRG 1 cut(s) 596
BmgBI CACGTC 2 cut(s) 180, 498
BmiI GGNNCC 1 cut(s) 476
BmtI GCTAGC 1 cut(s) 230
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 6 cut(s) 385, 417, 387, 419, 610, 642
Bpu14I TTCGAA 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 317
BseGI GGATG 4 cut(s) 132, 507, 526, 544
BseMII CTCAG 1 cut(s) 90
BseRI GAGGAG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 131, 458, 521
BsgI GTGCAG 2 cut(s) 512, 528
Bsh1236I CGCG 2 cut(s) 380, 417
Bsh1285I CGRYCG 1 cut(s) 582
BshFI GGCC 1 cut(s) 327
BsiEI CGRYCG 1 cut(s) 582
BsiHKAI GWGCWC 1 cut(s) 664
BsiHKCI CYCGRG 1 cut(s) 596
BsiSI CCGG 3 cut(s) 189, 218, 318
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 365
Bsp1286I GDGCHC 1 cut(s) 664
Bsp143I GATC 4 cut(s) 68, 121, 398, 589
BspACI CCGC 5 cut(s) 275, 306, 342, 380, 438
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 89
BspFNI CGCG 2 cut(s) 380, 417
BspLI GGNNCC 1 cut(s) 476
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 2 cut(s) 76, 406
BspT104I TTCGAA 1 cut(s) 365
BssMI GATC 4 cut(s) 68, 121, 398, 589
BssNI GRCGYC 1 cut(s) 168
Bst4CI ACNGT 3 cut(s) 10, 215, 583
Bst6I CTCTTC 2 cut(s) 44, 602
BstACI GRCGYC 1 cut(s) 168
BstBAI YACGTR 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 365
BstC8I GCNNGC 2 cut(s) 228, 237
BstDEI CTNAG 1 cut(s) 76
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 4 cut(s) 132, 507, 526, 544
BstFNI CGCG 2 cut(s) 380, 417
BstKTI GATC 4 cut(s) 71, 124, 401, 592
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 4 cut(s) 68, 121, 398, 589
BstMCI CGRYCG 1 cut(s) 582
BstMWI GCNNNNNNNGC 4 cut(s) 227, 232, 236, 435
BstPI GGTNACC 1 cut(s) 4
BstUI CGCG 2 cut(s) 380, 417
BstV1I GCAGC 3 cut(s) 131, 458, 521
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 1 cut(s) 327
BtrI CACGTC 2 cut(s) 180, 498
BtsCI GGATG 4 cut(s) 132, 507, 526, 544
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 228, 237
CseI GACGC 1 cut(s) 386
Csp6I GTAC 1 cut(s) 534
CviAII CATG 2 cut(s) 421, 666
CviQI GTAC 1 cut(s) 534
DdeI CTNAG 1 cut(s) 76
DpnI GATC 4 cut(s) 70, 123, 400, 591
DpnII GATC 4 cut(s) 68, 121, 398, 589
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 44, 602
EarI CTCTTC 2 cut(s) 44, 602
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 664
Eco53kI GAGCTC 1 cut(s) 662
Eco72I CACGTG 1 cut(s) 531
Eco88I CYCGRG 1 cut(s) 596
Eco91I GGTNACC 1 cut(s) 4
EcoICRI GAGCTC 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 4
EcoT38I GRGCYC 1 cut(s) 664
FaeI CATG 2 cut(s) 424, 669
FaiI YATR 4 cut(s) 160, 422, 554, 667
FatI CATG 2 cut(s) 420, 665
Fnu4HI GCNGC 6 cut(s) 145, 276, 306, 439, 447, 510
FokI GGATG 4 cut(s) 119, 514, 533, 551
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 6 cut(s) 145, 276, 306, 439, 447, 510
FspBI CTAG 2 cut(s) 125, 227
GluI GCNGC 6 cut(s) 145, 276, 306, 439, 447, 510
HaeIII GGCC 1 cut(s) 327
HapII CCGG 3 cut(s) 189, 218, 318
HgaI GACGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 168
Hin1II CATG 2 cut(s) 424, 669
HincII GTYRAC 2 cut(s) 410, 640
HindII GTYRAC 2 cut(s) 410, 640
HpaII CCGG 3 cut(s) 189, 218, 318
HphI GGTGA 2 cut(s) 37, 332
Hpy166II GTNNAC 4 cut(s) 385, 410, 534, 640
Hpy188I TCNGA 4 cut(s) 68, 137, 353, 453
Hpy188III TCNNGA 5 cut(s) 58, 125, 164, 209, 478
Hpy8I GTNNAC 4 cut(s) 385, 410, 534, 640
Hpy99I CGWCG 3 cut(s) 31, 34, 374
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 3 cut(s) 10, 215, 583
HpyCH4IV ACGT 8 cut(s) 32, 168, 179, 369, 467, 489, 497, 530
HpyCH4V TGCA 3 cut(s) 147, 493, 509
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 232, 236, 435
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 8 cut(s) 32, 168, 179, 369, 467, 489, 497, 530
Hsp92I GRCGYC 1 cut(s) 168
Hsp92II CATG 2 cut(s) 424, 669
Kzo9I GATC 4 cut(s) 68, 121, 398, 589
LmnI GCTCC 2 cut(s) 659, 667
LpnPI CCDG 5 cut(s) 177, 202, 231, 331, 491
Lsp1109I GCAGC 3 cut(s) 131, 458, 521
MaeI CTAG 2 cut(s) 125, 227
MaeII ACGT 8 cut(s) 32, 168, 179, 369, 467, 489, 497, 530
MaeIII GTNAC 4 cut(s) 4, 20, 180, 485
MalI GATC 4 cut(s) 70, 123, 400, 591
MboI GATC 4 cut(s) 68, 121, 398, 589
MboII GAAGA 5 cut(s) 31, 34, 386, 560, 619
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 664
MlsI TGGCCA 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 327
Mox20I TGGCCA 1 cut(s) 327
MscI TGGCCA 1 cut(s) 327
MseI TTAA 1 cut(s) 573
Msp20I TGGCCA 1 cut(s) 327
MspI CCGG 3 cut(s) 189, 218, 318
MvnI CGCG 2 cut(s) 380, 417
MwoI GCNNNNNNNGC 4 cut(s) 227, 232, 236, 435
NdeII GATC 4 cut(s) 68, 121, 398, 589
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 2 cut(s) 424, 669
NlaIV GGNNCC 1 cut(s) 476
NmuCI GTSAC 2 cut(s) 20, 180
NspV TTCGAA 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 596
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 243, 384, 464
PkrI GCNGC 6 cut(s) 146, 277, 307, 440, 448, 511
PmaCI CACGTG 1 cut(s) 531
PmlI CACGTG 1 cut(s) 531
Ppu21I YACGTR 1 cut(s) 531
Psp124BI GAGCTC 1 cut(s) 664
Psp1406I AACGTT 1 cut(s) 467
PspCI CACGTG 1 cut(s) 531
PspEI GGTNACC 1 cut(s) 4
PspN4I GGNNCC 1 cut(s) 476
PspXI VCTCGAGB 1 cut(s) 596
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 1 cut(s) 535
RsaNI GTAC 1 cut(s) 534
SacI GAGCTC 1 cut(s) 664
SaqAI TTAA 1 cut(s) 573
SatI GCNGC 6 cut(s) 145, 276, 306, 439, 447, 510
Sau3AI GATC 4 cut(s) 68, 121, 398, 589
SduI GDGCHC 1 cut(s) 664
Sfr274I CTCGAG 1 cut(s) 596
SfuI TTCGAA 1 cut(s) 365
SlaI CTCGAG 1 cut(s) 596
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
SsiI CCGC 5 cut(s) 275, 306, 342, 380, 438
SspMI CTAG 2 cut(s) 125, 227
SstI GAGCTC 1 cut(s) 664
TaaI ACNGT 3 cut(s) 10, 215, 583
TaiI ACGT 8 cut(s) 35, 171, 182, 372, 470, 492, 500, 533
TaqI TCGA 3 cut(s) 365, 372, 597
TauI GCSGC 3 cut(s) 278, 308, 441
Tru1I TTAA 1 cut(s) 573
Tru9I TTAA 1 cut(s) 573
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 2 cut(s) 20, 180
TseI GCWGC 3 cut(s) 144, 446, 509
Tsp45I GTSAC 2 cut(s) 20, 180
TspDTI ATGAA 2 cut(s) 121, 437
TspGWI ACGGA 4 cut(s) 74, 395, 533, 551
TspRI CASTG 1 cut(s) 277
XbaI TCTAGA 1 cut(s) 124
XhoI CTCGAG 1 cut(s) 596
XspI CTAG 2 cut(s) 125, 227
ZraI GACGTC 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.