FvH4_4g33960

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004993
Physical Location & Seq
Forward (+)
31983530 .. 31984817
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33960.t1

Sequence Viewer

Length: 612 bp
ATGGAAAACCTTCTCTCCCTCACCTCATTCTCACTCCTCATCTCCTTCCTCCTCCTCCGCTCACCCTCAGCCGTCGGATTCCCCGCCCCCGCCCCCTCCAATAACGACTCTGATTTTATCCGCACCAGCTGCGGCACCACCCTCTACCCCGAGCTCTGCTACACCTCCCTCTCCCGCTACGCCAACGCCGTCCAAAGCAGCCCTGCTCAGCTCGCCAAGGTCGCCATCGGCGTCAGCCTCTCCAATGCCAAGCGCATGGCCGCCTACGTCTCCAACCTCTCACGTCACGCCGACTACGGCTCCGACCACCGGTCCGCCGCCGCCCTCCACGACTGCTTCAGTAACTTCGACGATGCGGTCGATCAGATACGCGACTCGCTCAGCCAGATGAAGACGCTCGGGTCAGGCCAAGGGTCGGTAAGGTTTCAGATGAGCAACGTGCAGACGTGGATGAGTGCGGCGCTGACGGACCAGGAGACGTGTACGGACGGGTTTCAGGACGTGCCGGACGGGACGGTGAAGTCGGAGGTGGTGAAGCGGAGCGAGAAAGTGAAGAAGGTCACGAGTAATGCGCTGGCGCTCGTCAACTGCGTGGCGGAGAAGAGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

204

Amino Acids

21.78

Weight (kDa)

6.28

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 194 1.3e-44 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 356, 400, 520
AccB1I GGYRCC 1 cut(s) 134
AccBSI CCGCTC 2 cut(s) 60, 606
AccII CGCG 1 cut(s) 372
AcoI YGGCCR 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 322
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 309, 415
AflIII ACRYGT 1 cut(s) 479
AgeI ACCGGT 1 cut(s) 309
AhdI GACNNNNNGTC 1 cut(s) 310
AjiI CACGTC 4 cut(s) 284, 447, 480, 502
AjnI CCWGG 1 cut(s) 471
AluBI AGCT 3 cut(s) 129, 154, 211
AluI AGCT 3 cut(s) 129, 154, 211
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 2 cut(s) 274, 470
Ama87I CYCGRG 2 cut(s) 149, 398
AoxI GGCC 2 cut(s) 258, 406
ApeKI GCWGC 2 cut(s) 129, 198
AsiGI ACCGGT 1 cut(s) 309
Asp700I GAANNNNTTC 1 cut(s) 9
AspLEI GCGC 4 cut(s) 255, 463, 574, 580
AspS9I GGNCC 2 cut(s) 312, 469
AsuHPI GGTGA 4 cut(s) 13, 54, 529, 544
AvaI CYCGRG 2 cut(s) 149, 398
AvaII GGWCC 2 cut(s) 312, 469
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 67
BbvI GCAGC 2 cut(s) 116, 210
BccI CCATC 1 cut(s) 233
BceAI ACGGC 3 cut(s) 56, 173, 313
BciT130I CCWGG 1 cut(s) 473
BcoDI GTCTC 2 cut(s) 274, 470
BfoI RGCGCY 2 cut(s) 464, 581
BisI GCNGC 7 cut(s) 130, 133, 199, 261, 318, 321, 459
BlpI GCTNAGC 2 cut(s) 207, 380
BlsI GCNGC 7 cut(s) 131, 134, 200, 262, 319, 322, 460
Bme1390I CCNGG 1 cut(s) 473
Bme18I GGWCC 2 cut(s) 312, 469
BmeRI GACNNNNNGTC 1 cut(s) 310
BmeT110I CYCGRG 2 cut(s) 149, 398
BmgBI CACGTC 4 cut(s) 284, 447, 480, 502
BmgT120I GGNCC 2 cut(s) 312, 469
BmiI GGNNCC 2 cut(s) 136, 301
BmrFI CCNGG 1 cut(s) 473
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 398
Bpu10I CCTNAGC 1 cut(s) 67
Bpu1102I GCTNAGC 2 cut(s) 207, 380
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 216, 409
BsaWI WCCGGW 1 cut(s) 309
BsaXI ACNNNNNCTCC 3 cut(s) 29, 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 415
Bse118I RCCGGY 1 cut(s) 309
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 2 cut(s) 216, 409
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 309, 415
BseMII CTCAG 3 cut(s) 81, 221, 394
BseRI GAGGAG 3 cut(s) 26, 41, 44
BseXI GCAGC 2 cut(s) 116, 210
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 2 cut(s) 260, 408
BshNI GGYRCC 1 cut(s) 134
BshTI ACCGGT 1 cut(s) 309
BsiEI CGRYCG 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 2 cut(s) 149, 398
BsiSI CCGG 2 cut(s) 310, 506
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 2 cut(s) 309, 415
BsmAI GTCTC 2 cut(s) 274, 470
BsmBI CGTCTC 2 cut(s) 274, 470
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 2 cut(s) 260, 408
BsoBI CYCGRG 2 cut(s) 149, 398
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 1 cut(s) 361
Bsp1720I GCTNAGC 2 cut(s) 207, 380
BspANI GGCC 2 cut(s) 260, 408
BspCNI CTCAG 3 cut(s) 80, 220, 393
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 2 cut(s) 136, 301
BspQI GCTCTTC 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 134
BsrBI CCGCTC 2 cut(s) 60, 606
BsrFI RCCGGY 1 cut(s) 309
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 216, 409
BssMI GATC 1 cut(s) 361
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 562
BssT1I CCWWGG 2 cut(s) 216, 409
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 1 cut(s) 517
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 129
BstC8I GCNNGC 2 cut(s) 213, 576
BstDEI CTNAG 3 cut(s) 67, 207, 380
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 372
BstH2I RGCGCY 2 cut(s) 464, 581
BstHHI GCGC 4 cut(s) 255, 463, 574, 580
BstKTI GATC 1 cut(s) 364
BstMAI GTCTC 2 cut(s) 274, 470
BstMBI GATC 1 cut(s) 361
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 3 cut(s) 129, 212, 221
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 471
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 116, 210
BstV2I GAAGAC 1 cut(s) 398
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 2 cut(s) 260, 408
BtrI CACGTC 4 cut(s) 284, 447, 480, 502
BtsCI GGATG 1 cut(s) 456
Cac8I GCNNGC 2 cut(s) 213, 576
CfoI GCGC 4 cut(s) 255, 463, 574, 580
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 2 cut(s) 312, 469
CpoI CGGWCCG 1 cut(s) 312
CseI GACGC 2 cut(s) 220, 403
Csp6I GTAC 1 cut(s) 483
CspAI ACCGGT 1 cut(s) 309
CspI CGGWCCG 1 cut(s) 312
CviAII CATG 1 cut(s) 256
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 3 cut(s) 67, 207, 380
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
DrdI GACNNNNNNGTC 3 cut(s) 356, 400, 520
DriI GACNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 3 cut(s) 356, 400, 520
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 596
Eam1105I GACNNNNNGTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 2 cut(s) 304, 611
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 2 cut(s) 216, 409
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 312, 469
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 322
Eco88I CYCGRG 2 cut(s) 149, 398
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 216, 409
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 2 cut(s) 216, 409
Esp3I CGTCTC 2 cut(s) 274, 470
FaeI CATG 1 cut(s) 259
FaiI YATR 1 cut(s) 257
FaqI GGGAC 1 cut(s) 526
FatI CATG 1 cut(s) 255
FauI CCCGC 3 cut(s) 91, 97, 182
Fnu4HI GCNGC 7 cut(s) 130, 133, 199, 261, 318, 321, 459
FokI GGATG 1 cut(s) 463
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 7 cut(s) 130, 133, 199, 261, 318, 321, 459
GlaI GCGC 4 cut(s) 254, 462, 573, 579
GluI GCNGC 7 cut(s) 130, 133, 199, 261, 318, 321, 459
HaeII RGCGCY 2 cut(s) 464, 581
HaeIII GGCC 2 cut(s) 260, 408
HapII CCGG 2 cut(s) 310, 506
HgaI GACGC 2 cut(s) 220, 403
HhaI GCGC 4 cut(s) 255, 463, 574, 580
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 4 cut(s) 253, 461, 572, 578
HinP1I GCGC 4 cut(s) 253, 461, 572, 578
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 3 cut(s) 78, 107, 374
HpaII CCGG 2 cut(s) 310, 506
HphI GGTGA 4 cut(s) 13, 54, 529, 544
Hpy166II GTNNAC 2 cut(s) 483, 586
Hpy188I TCNGA 6 cut(s) 77, 112, 304, 366, 429, 526
Hpy188III TCNNGA 2 cut(s) 497, 562
Hpy8I GTNNAC 2 cut(s) 483, 586
Hpy99I CGWCG 2 cut(s) 77, 353
HpyAV CCTTC 3 cut(s) 20, 55, 550
HpyCH4III ACNGT 1 cut(s) 517
HpyCH4IV ACGT 6 cut(s) 267, 283, 438, 446, 479, 501
HpyCH4V TGCA 1 cut(s) 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 212, 221
HpyF3I CTNAG 3 cut(s) 67, 207, 380
HpySE526I ACGT 6 cut(s) 267, 283, 438, 446, 479, 501
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 4 cut(s) 253, 461, 572, 578
Kzo9I GATC 1 cut(s) 361
LguI GCTCTTC 1 cut(s) 596
LmnI GCTCC 2 cut(s) 305, 540
Lsp1109I GCAGC 2 cut(s) 116, 210
LweI GCATC 1 cut(s) 343
MaeII ACGT 6 cut(s) 267, 283, 438, 446, 479, 501
MaeIII GTNAC 3 cut(s) 284, 341, 559
MalI GATC 1 cut(s) 363
MbiI CCGCTC 2 cut(s) 60, 606
MboI GATC 1 cut(s) 361
MboII GAAGA 2 cut(s) 403, 565
MhlI GDGCHC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 101, 368
MmeI TCCRAC 4 cut(s) 55, 297, 327, 504
MroXI GAANNNNTTC 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 2 cut(s) 310, 506
MspR9I CCNGG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 129, 212, 221
NdeII GATC 1 cut(s) 361
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 136, 301
NmuCI GTSAC 2 cut(s) 284, 559
PciSI GCTCTTC 1 cut(s) 596
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 228, 357, 588
PdmI GAANNNNTTC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 309
PkrI GCNGC 7 cut(s) 131, 134, 200, 262, 319, 322, 460
PleI GAGTC 2 cut(s) 101, 368
PpsI GAGTC 2 cut(s) 101, 368
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 2 cut(s) 136, 301
PspPI GGNCC 2 cut(s) 312, 469
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
Rsr2I CGGWCCG 1 cut(s) 312
RsrII CGGWCCG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 596
SatI GCNGC 7 cut(s) 130, 133, 199, 261, 318, 321, 459
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 2 cut(s) 312, 469
SchI GAGTC 2 cut(s) 101, 368
ScrFI CCNGG 1 cut(s) 473
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 1 cut(s) 343
SinI GGWCC 2 cut(s) 312, 469
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 471
StyI CCWWGG 2 cut(s) 216, 409
TaaI ACNGT 1 cut(s) 517
TaiI ACGT 6 cut(s) 270, 286, 441, 449, 482, 504
TaqI TCGA 2 cut(s) 348, 360
TauI GCSGC 5 cut(s) 135, 263, 320, 323, 461
TfiI GAWTC 1 cut(s) 78
TseFI GTSAC 2 cut(s) 284, 559
TseI GCWGC 2 cut(s) 129, 198
Tsp45I GTSAC 2 cut(s) 284, 559
TspDTI ATGAA 1 cut(s) 404
TspGWI ACGGA 2 cut(s) 482, 500
VpaK11BI GGWCC 2 cut(s) 312, 469
XmnI GAANNNNTTC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.