Rorug04G0346600

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004993
Physical Location & Seq
Reverse (-)
51872965 .. 51873707
743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0346600.1

Sequence Viewer

Length: 636 bp
ATGCACTTTGGCCGGCCCAAAACCACGGTGGGGTCCCGCCACTCCTCGCTGGCGGAGGAGGCCCTGCAGAGCGCAATCCAGTTCGGTAAGGATGATTTGGCCCTTGATGATGTTTTGCTTAGTTTCGAGTCTAGGCTTTGTGGGTCGGATGATTACACTTTTTTGCTTAGAGAGCTTGGGAATAGAGGTGAGTGTTGGAAGGCTATAAGGTGTTTCGAGTTTGCAGTGAGGAGGGAGAGAAGGAGAACTGAGCAGGGGAAATTAGCTAGCTCTATGATTAGTACCTATAATACGCTTTTGGATGCAATTTCTAAAGGTGGGCAGATGGATTTGGCATATCAGATTATGTCAGAGATGCCTTCAAAGAATATTTTGCCTAATGTGGTGACTTATAGTACTATGATCGATGGTTATGCGAAGGCTGGTAGATTAGAAGATGCACTCAGTTTATTTAATGAAATGAAGTTTTTGGCCATTGGTCTGGATAGAGTTCTGTATAATACATTGCTGTCACTTTACGGGAAGCTTGGCAGGTTTGCAGAGGCTTTGAAGAAGAAGAAGAGAGGTCGAGGGAGAGAGAGAGACCGTGCGGCCAGGAGAGAGGGAGAGAGGGAGGGAGAGAGAGAGAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

211

Amino Acids

23.99

Weight (kDa)

9.38

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 87 - 178 3.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 93 - 140 4.1e-16 PPR repeat family
PPR_3 PF13812 93 - 136 2.6e-07 Pentatricopeptide repeat domain
PPR_3 PF13812 115 - 175 2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 122 - 155 1.8e-14 PPR repeat
PPR_2 PF13041 126 - 156 3.7e-11 PPR repeat family
PPR PF01535 129 - 155 7.7e-11 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 522
AciI CCGC 3 cut(s) 37, 53, 590
AcoI YGGCCR 3 cut(s) 10, 471, 591
AfaI GTAC 2 cut(s) 283, 397
AfiI CCNNNNNNNGG 1 cut(s) 30
AgsI TTSAA 2 cut(s) 363, 550
AjnI CCWGG 1 cut(s) 593
AluBI AGCT 4 cut(s) 175, 266, 270, 526
AluI AGCT 4 cut(s) 175, 266, 270, 526
Alw26I GTCTC 1 cut(s) 576
AoxI GGCC 6 cut(s) 10, 14, 60, 99, 471, 591
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 4 cut(s) 15, 33, 61, 100
AsuHPI GGTGA 2 cut(s) 200, 397
AsuNHI GCTAGC 1 cut(s) 266
AvaII GGWCC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 473
BccI CCATC 2 cut(s) 319, 401
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 1 cut(s) 595
BcoDI GTCTC 1 cut(s) 576
BfaI CTAG 2 cut(s) 132, 267
BfmI CTRYAG 1 cut(s) 65
BfuAI ACCTGC 1 cut(s) 522
BisI GCNGC 1 cut(s) 591
BlsI GCNGC 1 cut(s) 592
BmcAI AGTACT 1 cut(s) 397
Bme1390I CCNGG 1 cut(s) 595
Bme18I GGWCC 1 cut(s) 33
BmgT120I GGNCC 4 cut(s) 15, 33, 61, 100
BmiI GGNNCC 2 cut(s) 34, 35
BmrFI CCNGG 1 cut(s) 595
BmsI GCATC 3 cut(s) 292, 345, 427
BmtI GCTAGC 1 cut(s) 270
Bsa29I ATCGAT 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 576
BsaJI CCNNGG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 503
BseBI CCWGG 1 cut(s) 595
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 3 cut(s) 97, 154, 307
BseLI CCNNNNNNNGG 1 cut(s) 30
BseMI GCAATG 1 cut(s) 503
BseMII CTCAG 2 cut(s) 240, 457
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 3 cut(s) 34, 71, 244
BshFI GGCC 6 cut(s) 12, 16, 62, 101, 473, 593
BshVI ATCGAT 1 cut(s) 405
BsiSI CCGG 1 cut(s) 13
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 1 cut(s) 30
BsmAI GTCTC 1 cut(s) 576
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 6 cut(s) 12, 16, 62, 101, 473, 593
Bso31I GGTCTC 1 cut(s) 576
Bsp143I GATC 1 cut(s) 402
BspACI CCGC 3 cut(s) 37, 53, 590
BspANI GGCC 6 cut(s) 12, 16, 62, 101, 473, 593
BspCNI CTCAG 2 cut(s) 241, 456
BspDI ATCGAT 1 cut(s) 405
BspLI GGNNCC 2 cut(s) 34, 35
BspMAI CTGCAG 1 cut(s) 69
BspMI ACCTGC 1 cut(s) 522
BspOI GCTAGC 1 cut(s) 270
BspTNI GGTCTC 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 503
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 595
Bst4CI ACNGT 2 cut(s) 28, 587
Bst6I CTCTTC 1 cut(s) 554
BstC8I GCNNGC 3 cut(s) 14, 51, 268
BstDEI CTNAG 4 cut(s) 119, 167, 249, 443
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 97, 154, 307
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 1 cut(s) 405
BstMAI GTCTC 1 cut(s) 576
BstMBI GATC 1 cut(s) 402
BstMWI GCNNNNNNNGC 2 cut(s) 59, 172
BstNI CCWGG 1 cut(s) 595
BstSCI CCNGG 1 cut(s) 593
BstSFI CTRYAG 1 cut(s) 65
BstXI CCANNNNNNTGG 1 cut(s) 481
Bsu15I ATCGAT 1 cut(s) 405
BsuRI GGCC 6 cut(s) 12, 16, 62, 101, 473, 593
BsuTUI ATCGAT 1 cut(s) 405
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 3 cut(s) 97, 154, 307
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 14, 51, 268
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 4 cut(s) 15, 33, 61, 100
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 282, 396
CviQI GTAC 2 cut(s) 282, 396
DdeI CTNAG 4 cut(s) 119, 167, 249, 443
DpnI GATC 1 cut(s) 404
DpnII GATC 1 cut(s) 402
EaeI YGGCCR 3 cut(s) 10, 471, 591
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
EciI GGCGGA 1 cut(s) 68
Eco31I GGTCTC 1 cut(s) 576
Eco47I GGWCC 1 cut(s) 33
EcoO109I RGGNCCY 2 cut(s) 33, 61
EcoRII CCWGG 1 cut(s) 593
FaqI GGGAC 1 cut(s) 19
FauI CCCGC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 591
FokI GGATG 3 cut(s) 104, 161, 314
FseI GGCCGGCC 1 cut(s) 16
Fsp4HI GCNGC 1 cut(s) 591
FspBI CTAG 2 cut(s) 132, 267
GlaI GCGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 591
HaeIII GGCC 6 cut(s) 12, 16, 62, 101, 473, 593
HapII CCGG 1 cut(s) 13
HhaI GCGC 1 cut(s) 74
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 1 cut(s) 128
HpaII CCGG 1 cut(s) 13
HphI GGTGA 2 cut(s) 200, 397
Hpy188I TCNGA 3 cut(s) 148, 342, 352
Hpy188III TCNNGA 1 cut(s) 482
HpyAV CCTTC 4 cut(s) 193, 234, 369, 412
HpyCH4III ACNGT 2 cut(s) 28, 587
HpyCH4V TGCA 6 cut(s) 4, 67, 224, 305, 440, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 172
HpyF3I CTNAG 4 cut(s) 119, 167, 249, 443
HspAI GCGC 1 cut(s) 72
KflI GGGWCCC 1 cut(s) 33
KroI GCCGGC 1 cut(s) 12
KroNI GCCGGC 1 cut(s) 14
Kzo9I GATC 1 cut(s) 402
LweI GCATC 3 cut(s) 292, 345, 427
MaeI CTAG 2 cut(s) 132, 267
MaeIII GTNAC 2 cut(s) 385, 510
MalI GATC 1 cut(s) 404
MboI GATC 1 cut(s) 402
MboII GAAGA 5 cut(s) 446, 562, 565, 568, 571
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 260, 306
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 2 cut(s) 126, 176
Mox20I TGGCCA 1 cut(s) 473
MroNI GCCGGC 1 cut(s) 12
MscI TGGCCA 1 cut(s) 473
MseI TTAA 1 cut(s) 453
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 595
MvaI CCWGG 1 cut(s) 595
MwoI GCNNNNNNNGC 2 cut(s) 59, 172
NaeI GCCGGC 1 cut(s) 14
NdeII GATC 1 cut(s) 402
NgoMIV GCCGGC 1 cut(s) 12
NheI GCTAGC 1 cut(s) 266
NlaIV GGNNCC 2 cut(s) 34, 35
NmuCI GTSAC 2 cut(s) 385, 510
PdiI GCCGGC 1 cut(s) 14
PkrI GCNGC 1 cut(s) 592
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PpuMI RGGWCCY 1 cut(s) 33
Psp5II RGGWCCY 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 593
PspGI CCWGG 1 cut(s) 593
PspN4I GGNNCC 2 cut(s) 34, 35
PspPI GGNCC 4 cut(s) 15, 33, 61, 100
PspPPI RGGWCCY 1 cut(s) 33
PstI CTGCAG 1 cut(s) 69
RigI GGCCGGCC 1 cut(s) 16
RsaI GTAC 2 cut(s) 283, 397
RsaNI GTAC 2 cut(s) 282, 396
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 1 cut(s) 591
Sau3AI GATC 1 cut(s) 402
Sau96I GGNCC 4 cut(s) 15, 33, 61, 100
ScaI AGTACT 1 cut(s) 397
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 595
SfaNI GCATC 3 cut(s) 292, 345, 427
SfcI CTRYAG 1 cut(s) 65
SinI GGWCC 1 cut(s) 33
Sse9I AATT 2 cut(s) 260, 306
SsiI CCGC 3 cut(s) 37, 53, 590
SspI AATATT 1 cut(s) 370
SspMI CTAG 2 cut(s) 132, 267
StyD4I CCNGG 1 cut(s) 593
TaaI ACNGT 2 cut(s) 28, 587
TaqI TCGA 4 cut(s) 126, 216, 405, 568
TasI AATT 2 cut(s) 260, 306
TatI WGTACW 1 cut(s) 395
TauI GCSGC 1 cut(s) 593
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 2 cut(s) 385, 510
Tsp45I GTSAC 2 cut(s) 385, 510
TspDTI ATGAA 2 cut(s) 471, 476
TspRI CASTG 1 cut(s) 231
VpaK11BI GGWCC 1 cut(s) 33
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 2 cut(s) 132, 267
ZrmI AGTACT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.