AT1G23205

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004993
Physical Location & Seq
Reverse (-)
8233953 .. 8235019
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23205.1

Sequence Viewer

Length: 618 bp
ATGAAACTTTCTCTTCATCAACCACTTCTCTTCTTCTTCTTAGCCAGCGTCCTCCCATTAATCCTGACCGTCCATTCTCAATCAGACGACTCAGATTTCATCCGTACTAGCTGCAACACCACATTATACCCTGACCTATGCTTCTCTTCTCTTTCCTCCTTCTCTAGCTCTGTTCACAACGATCCAGCCCTCTTGGCTCGAGCCGCCATCTCCGTCACTCTCACCAAGACTCTCGACTTAGCCTCATACCTCGCCAATATCACCACTCTCCAGCCAGAGAGCAACGAAGATGGTGCTCACCACCCAACCGCAGCCGCTGTTTTTCACGACTGCTTCGACAATCTTAAAGACGCGGTCGAAGAAATGAAAGGCTCGATGAAACAGATGAGGGAGCTGGTCTCGACTGGCTCGCTCGAGTCCTTCAGGTTCCAAATGAGTAACGTGCAGACGTGGCTCAGTGCGGCTTTAACTGATGAGGAGACTTGTACGGACGGATTTAAAGACATTCACGATGAGCCGAGGAAGGATGACATATGCGCACGGGTCGATGACGTTAAAAAGATGACTAGTAATGCGTTGGCTCTAGTTAATCGATGTGTTGATAAGGCCATACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

205

Amino Acids

22.68

Weight (kDa)

5.02

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 195 2.7e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 538
AccII CGCG 1 cut(s) 353
AciI CCGC 5 cut(s) 204, 309, 315, 353, 461
AclWI GGATC 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 406
AfaI GTAC 2 cut(s) 106, 487
AhlI ACTAGT 1 cut(s) 566
AjiI CACGTC 1 cut(s) 450
AluBI AGCT 3 cut(s) 111, 168, 394
AluI AGCT 3 cut(s) 111, 168, 394
Alw21I GWGCWC 1 cut(s) 298
Alw26I GTCTC 2 cut(s) 403, 473
AlwI GGATC 1 cut(s) 176
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 2 cut(s) 198, 413
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 2 cut(s) 111, 311
AseI ATTAAT 1 cut(s) 59
AspLEI GCGC 1 cut(s) 539
AsuHPI GGTGA 3 cut(s) 214, 253, 290
AvaI CYCGRG 2 cut(s) 198, 413
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 2 cut(s) 98, 323
BccI CCATC 2 cut(s) 215, 284
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BcoDI GTCTC 2 cut(s) 403, 473
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 4 cut(s) 108, 165, 567, 584
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 5 cut(s) 112, 204, 312, 315, 462
BlsI GCNGC 5 cut(s) 113, 205, 313, 316, 463
BmeT110I CYCGRG 2 cut(s) 198, 413
BmgBI CACGTC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpmI CTGGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 592
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 409
BseCI ATCGAT 1 cut(s) 592
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 2 cut(s) 99, 532
BseMII CTCAG 2 cut(s) 105, 469
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 2 cut(s) 98, 323
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 608
BshVI ATCGAT 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 298
BsiHKCI CYCGRG 2 cut(s) 198, 413
BsmAI GTCTC 2 cut(s) 403, 473
BsnI GGCC 1 cut(s) 608
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 2 cut(s) 198, 413
Bsp1286I GDGCHC 1 cut(s) 298
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 5 cut(s) 204, 309, 315, 353, 461
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 2 cut(s) 104, 468
BspDI ATCGAT 1 cut(s) 592
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 428
BspPI GGATC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 403
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 3 cut(s) 18, 35, 151
BstC8I GCNNGC 2 cut(s) 46, 410
BstDEI CTNAG 4 cut(s) 40, 91, 238, 455
BstF5I GGATG 2 cut(s) 99, 532
BstFNI CGCG 1 cut(s) 353
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 2 cut(s) 403, 473
BstMBI GATC 1 cut(s) 181
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 194, 203, 451
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 98, 323
Bsu15I ATCGAT 1 cut(s) 592
BsuRI GGCC 1 cut(s) 608
BsuTUI ATCGAT 1 cut(s) 592
BtrI CACGTC 1 cut(s) 450
BtsCI GGATG 2 cut(s) 99, 532
BtsIMutI CAGTG 1 cut(s) 463
Cac8I GCNNGC 2 cut(s) 46, 410
CaiI CAGNNNCTG 1 cut(s) 317
CfoI GCGC 1 cut(s) 539
ClaI ATCGAT 1 cut(s) 592
CseI GACGC 2 cut(s) 37, 359
Csp6I GTAC 2 cut(s) 105, 486
CviQI GTAC 2 cut(s) 105, 486
DdeI CTNAG 4 cut(s) 40, 91, 238, 455
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
DraI TTTAAA 1 cut(s) 499
Eam1104I CTCTTC 3 cut(s) 18, 35, 151
EarI CTCTTC 3 cut(s) 18, 35, 151
Eco31I GGTCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 406
Eco88I CYCGRG 2 cut(s) 198, 413
FaiI YATR 6 cut(s) 127, 139, 247, 533, 535, 611
FauNDI CATATG 1 cut(s) 533
Fnu4HI GCNGC 5 cut(s) 112, 204, 312, 315, 462
FokI GGATG 2 cut(s) 86, 539
Fsp4HI GCNGC 5 cut(s) 112, 204, 312, 315, 462
FspAI RTGCGCAY 1 cut(s) 538
FspBI CTAG 4 cut(s) 108, 165, 567, 584
FspI TGCGCA 1 cut(s) 538
GlaI GCGC 1 cut(s) 538
GluI GCNGC 5 cut(s) 112, 204, 312, 315, 462
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 1 cut(s) 608
HgaI GACGC 2 cut(s) 37, 359
HhaI GCGC 1 cut(s) 539
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HinfI GANTC 3 cut(s) 89, 229, 416
HphI GGTGA 3 cut(s) 214, 253, 290
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 85, 94
Hpy188III TCNNGA 5 cut(s) 64, 233, 326, 400, 509
Hpy8I GTNNAC 1 cut(s) 175
HpyAV CCTTC 3 cut(s) 169, 430, 517
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 3 cut(s) 441, 449, 552
HpyCH4V TGCA 2 cut(s) 114, 445
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 203, 451
HpyF3I CTNAG 4 cut(s) 40, 91, 238, 455
HpySE526I ACGT 3 cut(s) 441, 449, 552
HspAI GCGC 1 cut(s) 537
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 9 cut(s) 58, 77, 144, 198, 284, 288, 380, 390, 409
Lsp1109I GCAGC 2 cut(s) 98, 323
MaeI CTAG 4 cut(s) 108, 165, 567, 584
MaeII ACGT 3 cut(s) 441, 449, 552
MaeIII GTNAC 2 cut(s) 214, 437
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 7 cut(s) 5, 22, 25, 28, 138, 299, 371
MhlI GDGCHC 1 cut(s) 298
MlyI GAGTC 3 cut(s) 83, 223, 425
MnlI CCTC 8 cut(s) 62, 166, 200, 253, 260, 381, 469, 513
MseI TTAA 6 cut(s) 59, 345, 467, 498, 555, 588
MspA1I CMGCKG 1 cut(s) 317
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 3 cut(s) 194, 203, 451
NdeI CATATG 1 cut(s) 533
NdeII GATC 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 428
NmeAIII GCCGAG 1 cut(s) 543
NmuCI GTSAC 1 cut(s) 214
NsbI TGCGCA 1 cut(s) 538
PaeR7I CTCGAG 2 cut(s) 198, 413
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 5 cut(s) 113, 205, 313, 316, 463
PleI GAGTC 3 cut(s) 83, 223, 424
PpsI GAGTC 3 cut(s) 83, 223, 424
PshBI ATTAAT 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 428
PspXI VCTCGAGB 2 cut(s) 198, 413
PstNI CAGNNNCTG 1 cut(s) 317
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 2 cut(s) 106, 487
RsaNI GTAC 2 cut(s) 105, 486
SaqAI TTAA 6 cut(s) 59, 345, 467, 498, 555, 588
SatI GCNGC 5 cut(s) 112, 204, 312, 315, 462
Sau3AI GATC 1 cut(s) 181
SchI GAGTC 3 cut(s) 83, 223, 425
SduI GDGCHC 1 cut(s) 298
SetI ASST 9 cut(s) 113, 138, 170, 252, 396, 428, 444, 452, 555
Sfr274I CTCGAG 2 cut(s) 198, 413
SlaI CTCGAG 2 cut(s) 198, 413
SmlI CTYRAG 2 cut(s) 198, 413
SmoI CTYRAG 2 cut(s) 198, 413
SpeI ACTAGT 1 cut(s) 566
SsiI CCGC 5 cut(s) 204, 309, 315, 353, 461
SspMI CTAG 4 cut(s) 108, 165, 567, 584
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 3 cut(s) 444, 452, 555
TaqI TCGA 9 cut(s) 199, 234, 336, 357, 374, 401, 414, 546, 592
TauI GCSGC 3 cut(s) 206, 317, 464
Tru1I TTAA 6 cut(s) 59, 345, 467, 498, 555, 588
Tru9I TTAA 6 cut(s) 59, 345, 467, 498, 555, 588
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 2 cut(s) 111, 311
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 5 cut(s) 5, 17, 88, 380, 392
TspGWI ACGGA 4 cut(s) 92, 202, 503, 507
TspRI CASTG 1 cut(s) 463
Tth111I GACNNNGTC 1 cut(s) 353
VspI ATTAAT 1 cut(s) 59
XhoI CTCGAG 2 cut(s) 198, 413
XspI CTAG 4 cut(s) 108, 165, 567, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.