FvH4_4g33950

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004993
Physical Location & Seq
Forward (+)
31981910 .. 31982768
859 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33950.t1

Sequence Viewer

Length: 606 bp
ATGAAAAACACTCAATGCCTCCTCCTCCTAGTCCTCCCTTTCATCTGCCTTCTCTTCCTCCATCCAACCTCAGCCGTCGGAGTCCCATTTCCAACCGCCGACCCTGCAAATTTCATCCGCACAAGCTGTAACACCACCCTATACCCCGACCTCTGCTACTCCTCCCTCTCACCTTTCACCAACAACGTCCAATCAAACCTCACCCGGCTTGCGAAGGCAGCCATCGCCGTGAGTCTAAGAAGTTCCAGACACACCATGATTCTCCTAACAAGCCTCTCCGCACAAGCCGACCCTAAAGTCGCCGGGGCTCTTCGGGACTGCTTCAGCAACTTCGGAGACGCCTTCGAAGAGATTAGAGAGTCCCTCGATCAGATGAAGACGCTGGGGTCGGGAACCGGGTCAGTTAGGTTTCAGCTGAGCAACGTCCAGACGTGGATAAGTGCAGCGCTTACGGACGCCGAGACGTGTACGGATGGATTTGAGGACGTTGAGGACGGCCCGATCAAGACGGAGGTAGTTAACAAGGCTGAGATGGTGAAGAAGATGATGAGTAATGCGCTCGCGATCGTTAATGGTCTTGCTAGTAGTAACGGCACACCACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

202

Amino Acids

21.58

Weight (kDa)

5.35

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 37 - 189 6.8e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 296, 385
AccII CGCG 1 cut(s) 563
AciI CCGC 3 cut(s) 96, 118, 279
AcsI RAATTY 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 307
AcyI GRCGYC 2 cut(s) 339, 456
AfaI GTAC 1 cut(s) 469
AfeI AGCGCT 1 cut(s) 447
AflIII ACRYGT 1 cut(s) 464
AjiI CACGTC 2 cut(s) 432, 465
AluBI AGCT 2 cut(s) 126, 415
AluI AGCT 2 cut(s) 126, 415
Alw26I GTCTC 2 cut(s) 330, 455
Aor51HI AGCGCT 1 cut(s) 447
AoxI GGCC 1 cut(s) 496
ApeKI GCWGC 2 cut(s) 218, 443
ApoI RAATTY 1 cut(s) 109
AspLEI GCGC 2 cut(s) 448, 559
AspS9I GGNCC 1 cut(s) 497
AsuC2I CCSGG 3 cut(s) 205, 304, 397
AsuHPI GGTGA 4 cut(s) 162, 169, 193, 547
AsuII TTCGAA 1 cut(s) 345
BanII GRGCYC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 230, 455
BccI CCATC 4 cut(s) 69, 230, 467, 526
BceAI ACGGC 3 cut(s) 59, 212, 511
BcnI CCSGG 3 cut(s) 205, 304, 397
BcoDI GTCTC 2 cut(s) 330, 455
BfaI CTAG 2 cut(s) 29, 582
BfoI RGCGCY 1 cut(s) 449
BisI GCNGC 2 cut(s) 219, 444
BlpI GCTNAGC 1 cut(s) 416
BlsI GCNGC 2 cut(s) 220, 445
Bme1390I CCNGG 3 cut(s) 205, 304, 397
BmgBI CACGTC 2 cut(s) 432, 465
BmgT120I GGNCC 1 cut(s) 497
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 3 cut(s) 205, 304, 397
BpiI GAAGAC 1 cut(s) 383
BplI GAGNNNNNCTC 2 cut(s) 348, 380
Bpu10I CCTNAGC 1 cut(s) 70
Bpu1102I GCTNAGC 1 cut(s) 416
Bpu14I TTCGAA 1 cut(s) 345
BpuMI CCSGG 3 cut(s) 205, 304, 397
BsaHI GRCGYC 2 cut(s) 339, 456
BsaJI CCNNGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 3 cut(s) 61, 114, 478
BseMII CTCAG 3 cut(s) 84, 407, 519
BseRI GAGGAG 3 cut(s) 11, 14, 151
BseXI GCAGC 2 cut(s) 230, 455
BseYI CCCAGC 1 cut(s) 382
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 567
BshFI GGCC 1 cut(s) 498
BsiEI CGRYCG 1 cut(s) 567
BsiSI CCGG 3 cut(s) 205, 303, 396
BslFI GGGAC 3 cut(s) 68, 329, 346
BsmAI GTCTC 2 cut(s) 330, 455
BsmBI CGTCTC 2 cut(s) 330, 455
BsmFI GGGAC 3 cut(s) 68, 329, 346
BsnI GGCC 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 3 cut(s) 367, 501, 564
Bsp1720I GCTNAGC 1 cut(s) 416
Bsp68I TCGCGA 1 cut(s) 563
BspACI CCGC 3 cut(s) 96, 118, 279
BspANI GGCC 1 cut(s) 498
BspCNI CTCAG 3 cut(s) 83, 408, 520
BspFNI CGCG 1 cut(s) 563
BspLI GGNNCC 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 315
BspT104I TTCGAA 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 3 cut(s) 367, 501, 564
BssNI GRCGYC 2 cut(s) 339, 456
Bst6I CTCTTC 3 cut(s) 59, 315, 342
BstACI GRCGYC 2 cut(s) 339, 456
BstBI TTCGAA 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 210, 561
BstDEI CTNAG 4 cut(s) 70, 236, 416, 528
BstF5I GGATG 3 cut(s) 61, 114, 478
BstFNI CGCG 1 cut(s) 563
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 2 cut(s) 448, 559
BstKTI GATC 3 cut(s) 370, 504, 567
BstMAI GTCTC 2 cut(s) 330, 455
BstMBI GATC 3 cut(s) 367, 501, 564
BstMCI CGRYCG 1 cut(s) 567
BstMWI GCNNNNNNNGC 3 cut(s) 104, 218, 224
BstSCI CCNGG 3 cut(s) 203, 302, 395
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 2 cut(s) 230, 455
BstV2I GAAGAC 1 cut(s) 383
BsuRI GGCC 1 cut(s) 498
BtgZI GCGATG 1 cut(s) 208
BtrI CACGTC 2 cut(s) 432, 465
BtsCI GGATG 3 cut(s) 61, 114, 478
BtuMI TCGCGA 1 cut(s) 563
Cac8I GCNNGC 2 cut(s) 210, 561
CfoI GCGC 2 cut(s) 448, 559
Cfr13I GGNCC 1 cut(s) 497
CseI GACGC 3 cut(s) 347, 388, 464
Csp6I GTAC 1 cut(s) 468
CviAII CATG 1 cut(s) 256
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 4 cut(s) 70, 236, 416, 528
DpnI GATC 3 cut(s) 369, 503, 566
DpnII GATC 3 cut(s) 367, 501, 564
DrdI GACNNNNNNGTC 2 cut(s) 296, 385
DseDI GACNNNNNNGTC 2 cut(s) 296, 385
Eam1104I CTCTTC 3 cut(s) 59, 315, 342
EarI CTCTTC 3 cut(s) 59, 315, 342
Eco24I GRGCYC 1 cut(s) 310
Eco47III AGCGCT 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 310
Esp3I CGTCTC 2 cut(s) 330, 455
FaeI CATG 1 cut(s) 259
FaiI YATR 2 cut(s) 142, 257
FaqI GGGAC 3 cut(s) 68, 329, 346
FatI CATG 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 219, 444
FokI GGATG 3 cut(s) 48, 101, 485
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 2 cut(s) 219, 444
FspBI CTAG 2 cut(s) 29, 582
GlaI GCGC 2 cut(s) 447, 558
GluI GCNGC 2 cut(s) 219, 444
GsaI CCCAGC 1 cut(s) 386
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 1 cut(s) 498
HapII CCGG 3 cut(s) 205, 303, 396
HgaI GACGC 3 cut(s) 347, 388, 464
HhaI GCGC 2 cut(s) 448, 559
Hin1I GRCGYC 2 cut(s) 339, 456
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 2 cut(s) 446, 557
HinP1I GCGC 2 cut(s) 446, 557
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 4 cut(s) 81, 232, 259, 359
HpaI GTTAAC 1 cut(s) 520
HpaII CCGG 3 cut(s) 205, 303, 396
HphI GGTGA 4 cut(s) 162, 169, 193, 547
Hpy166II GTNNAC 2 cut(s) 468, 520
Hpy188I TCNGA 3 cut(s) 80, 335, 372
Hpy188III TCNNGA 6 cut(s) 246, 314, 390, 427, 505, 562
Hpy8I GTNNAC 2 cut(s) 468, 520
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 59, 208, 352
HpyCH4IV ACGT 5 cut(s) 186, 423, 431, 464, 486
HpyCH4V TGCA 2 cut(s) 107, 443
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 218, 224
HpyF3I CTNAG 4 cut(s) 70, 236, 416, 528
HpySE526I ACGT 5 cut(s) 186, 423, 431, 464, 486
Hsp92I GRCGYC 2 cut(s) 339, 456
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 2 cut(s) 446, 557
KspAI GTTAAC 1 cut(s) 520
Kzo9I GATC 3 cut(s) 367, 501, 564
LguI GCTCTTC 1 cut(s) 315
LpnPI CCDG 7 cut(s) 117, 218, 259, 316, 368, 409, 440
Lsp1109I GCAGC 2 cut(s) 230, 455
MaeI CTAG 2 cut(s) 29, 582
MaeII ACGT 5 cut(s) 186, 423, 431, 464, 486
MaeIII GTNAC 2 cut(s) 128, 587
MalI GATC 3 cut(s) 369, 503, 566
MboI GATC 3 cut(s) 367, 501, 564
MboII GAAGA 6 cut(s) 46, 302, 359, 388, 550, 553
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 1 cut(s) 109
MlyI GAGTC 3 cut(s) 90, 241, 368
MmeI TCCRAC 3 cut(s) 58, 89, 116
MseI TTAA 2 cut(s) 519, 570
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 415
MspI CCGG 3 cut(s) 205, 303, 396
MspR9I CCNGG 3 cut(s) 205, 304, 397
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 3 cut(s) 104, 218, 224
NciI CCSGG 3 cut(s) 205, 304, 397
NdeII GATC 3 cut(s) 367, 501, 564
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 1 cut(s) 394
NmeAIII GCCGAG 1 cut(s) 484
NruI TCGCGA 1 cut(s) 563
NspV TTCGAA 1 cut(s) 345
PciSI GCTCTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 220, 445
Ple19I CGATCG 1 cut(s) 567
PleI GAGTC 3 cut(s) 89, 240, 367
PpsI GAGTC 3 cut(s) 89, 240, 367
PspFI CCCAGC 1 cut(s) 382
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 497
PvuI CGATCG 1 cut(s) 567
PvuII CAGCTG 1 cut(s) 415
RruI TCGCGA 1 cut(s) 563
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 2 cut(s) 519, 570
SatI GCNGC 2 cut(s) 219, 444
Sau3AI GATC 3 cut(s) 367, 501, 564
Sau96I GGNCC 1 cut(s) 497
SchI GAGTC 3 cut(s) 90, 241, 368
ScrFI CCNGG 3 cut(s) 205, 304, 397
SduI GDGCHC 1 cut(s) 310
SfuI TTCGAA 1 cut(s) 345
SmiMI CAYNNNNRTG 1 cut(s) 227
Sse9I AATT 1 cut(s) 109
SsiI CCGC 3 cut(s) 96, 118, 279
SspMI CTAG 2 cut(s) 29, 582
StyD4I CCNGG 3 cut(s) 203, 302, 395
TaiI ACGT 5 cut(s) 189, 426, 434, 467, 489
TaqI TCGA 2 cut(s) 345, 366
TasI AATT 1 cut(s) 109
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 519, 570
Tru9I TTAA 2 cut(s) 519, 570
TseI GCWGC 2 cut(s) 218, 443
TspDTI ATGAA 4 cut(s) 17, 31, 103, 389
TspGWI ACGGA 3 cut(s) 467, 485, 524
XapI RAATTY 1 cut(s) 109
XspI CTAG 2 cut(s) 29, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.