Rw4G034670

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004993
Physical Location & Seq
Forward (+)
61667429 .. 61668039
611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034670.1

Sequence Viewer

Length: 582 bp
ATGAAAAACCCTCAATCCGTCCTCCTACTCCTTCCTCTCATTTTCCTCTTCTTCCTCCGTCCAACCTTAGCCGTCGAATCCCCCTCCTCCTCTCCCACCCCCACCAACAACCCTGCAGATTTCATCCGCACAAGCTGCGCCACCACCCTATACCCCGAGCTCTGCGACTCCTCCCTCTCACCCTTCACCTACACCGTCCAGTCAAACCCTACTCGGCTTGCGATGGCAGCCATCGCTGTGAGCCTAGCCAATGCCAGACGCACGGCGACTTCCTCGCAAGCCTCTCCAAACAAGCCGACCCTAAACAACTTCGGAAAAGCCATCGACGAGATTACAGATTCACAAGAGCAGATGAAGACGTTGGGGTCGGGTTCCGGGTCGGTTAGGTTTCAGCTGAGCAACGTGCAGACTTGGATGAGTGCGGCCCTTACGGACGCGGAGACGTGTACGGATGGATTTGAGGACGTGGAGGACGGGCCGATCAAGACGGAGGTGGTGAACCAGGCTGAGATGGTGAAGAAGATGACAAGTAATGCGCTTGCGATCGTTAATGGTCTTGCTAGTAACGGCGCACCGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

193

Amino Acids

20.53

Weight (kDa)

4.8

Isoelectric Point (pI)

55.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 182 4.7e-32 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 364
AccII CGCG 1 cut(s) 437
AciI CCGC 4 cut(s) 127, 422, 437, 575
AfaI GTAC 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 443
AjiI CACGTC 2 cut(s) 444, 466
AjnI CCWGG 1 cut(s) 501
AluBI AGCT 3 cut(s) 135, 160, 394
AluI AGCT 3 cut(s) 135, 160, 394
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 423, 476
ApeKI GCWGC 2 cut(s) 135, 227
AspLEI GCGC 3 cut(s) 140, 538, 572
AspS9I GGNCC 2 cut(s) 424, 476
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 4 cut(s) 171, 178, 508, 526
AvaI CYCGRG 1 cut(s) 155
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 122, 239
BccI CCATC 5 cut(s) 217, 239, 329, 446, 505
BceAI ACGGC 2 cut(s) 56, 279
BciT130I CCWGG 1 cut(s) 503
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 2 cut(s) 245, 561
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 3 cut(s) 136, 228, 423
BlpI GCTNAGC 1 cut(s) 395
BlsI GCNGC 3 cut(s) 137, 229, 424
Bme1390I CCNGG 2 cut(s) 376, 503
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 2 cut(s) 444, 466
BmgT120I GGNCC 2 cut(s) 424, 476
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 2 cut(s) 376, 503
BpiI GAAGAC 1 cut(s) 362
Bpu10I CCTNAGC 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 376
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 1 cut(s) 503
BseGI GGATG 3 cut(s) 123, 420, 457
BseMII CTCAG 2 cut(s) 386, 498
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 76, 79, 160
BseXI GCAGC 2 cut(s) 122, 239
BsgI GTGCAG 1 cut(s) 425
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 546
BshFI GGCC 2 cut(s) 425, 478
BsiEI CGRYCG 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 375
BsmAI GTCTC 1 cut(s) 434
BsmBI CGTCTC 1 cut(s) 434
BsnI GGCC 2 cut(s) 425, 478
BsoBI CYCGRG 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 480, 543
Bsp1720I GCTNAGC 1 cut(s) 395
BspACI CCGC 4 cut(s) 127, 422, 437, 575
BspANI GGCC 2 cut(s) 425, 478
BspCNI CTCAG 2 cut(s) 387, 499
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 1 cut(s) 373
BspMAI CTGCAG 1 cut(s) 118
BsrI ACTGG 1 cut(s) 199
BssMI GATC 2 cut(s) 480, 543
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 219, 279, 540
BstDEI CTNAG 3 cut(s) 67, 395, 507
BstF5I GGATG 3 cut(s) 123, 420, 457
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 3 cut(s) 140, 538, 572
BstKTI GATC 2 cut(s) 483, 546
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 2 cut(s) 480, 543
BstMCI CGRYCG 1 cut(s) 546
BstMWI GCNNNNNNNGC 3 cut(s) 135, 227, 233
BstNI CCWGG 1 cut(s) 503
BstSCI CCNGG 2 cut(s) 374, 501
BstSFI CTRYAG 1 cut(s) 114
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 2 cut(s) 122, 239
BstV2I GAAGAC 1 cut(s) 362
BsuRI GGCC 2 cut(s) 425, 478
BtgZI GCGATG 2 cut(s) 217, 236
BtrI CACGTC 2 cut(s) 444, 466
BtsCI GGATG 3 cut(s) 123, 420, 457
Cac8I GCNNGC 3 cut(s) 219, 279, 540
CfoI GCGC 3 cut(s) 140, 538, 572
Cfr13I GGNCC 2 cut(s) 424, 476
CseI GACGC 2 cut(s) 267, 443
Csp6I GTAC 1 cut(s) 447
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 3 cut(s) 67, 395, 507
DpnI GATC 2 cut(s) 482, 545
DpnII GATC 2 cut(s) 480, 543
DrdI GACNNNNNNGTC 1 cut(s) 364
DseDI GACNNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 501
EcoT38I GRGCYC 1 cut(s) 162
Esp3I CGTCTC 1 cut(s) 434
FaiI YATR 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 136, 228, 423
FokI GGATG 3 cut(s) 110, 427, 464
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 136, 228, 423
FspBI CTAG 2 cut(s) 245, 561
GlaI GCGC 3 cut(s) 139, 537, 571
GluI GCNGC 3 cut(s) 136, 228, 423
HaeIII GGCC 2 cut(s) 425, 478
HapII CCGG 1 cut(s) 375
HgaI GACGC 2 cut(s) 267, 443
HhaI GCGC 3 cut(s) 140, 538, 572
Hin6I GCGC 3 cut(s) 138, 536, 570
HinP1I GCGC 3 cut(s) 138, 536, 570
HinfI GANTC 3 cut(s) 77, 167, 338
HpaII CCGG 1 cut(s) 375
HphI GGTGA 4 cut(s) 171, 178, 508, 526
Hpy166II GTNNAC 2 cut(s) 447, 499
Hpy188I TCNGA 1 cut(s) 314
Hpy188III TCNNGA 1 cut(s) 484
Hpy8I GTNNAC 2 cut(s) 447, 499
Hpy99I CGWCG 2 cut(s) 77, 329
HpyAV CCTTC 2 cut(s) 41, 193
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 4 cut(s) 359, 402, 443, 465
HpyCH4V TGCA 2 cut(s) 116, 406
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 227, 233
HpyF3I CTNAG 3 cut(s) 67, 395, 507
HpySE526I ACGT 4 cut(s) 359, 402, 443, 465
HspAI GCGC 3 cut(s) 138, 536, 570
Kzo9I GATC 2 cut(s) 480, 543
LpnPI CCDG 6 cut(s) 126, 212, 268, 388, 488, 515
Lsp1109I GCAGC 2 cut(s) 122, 239
MaeI CTAG 2 cut(s) 245, 561
MaeII ACGT 4 cut(s) 359, 402, 443, 465
MaeIII GTNAC 1 cut(s) 563
MalI GATC 2 cut(s) 482, 545
MboI GATC 2 cut(s) 480, 543
MboII GAAGA 5 cut(s) 40, 43, 367, 529, 532
MhlI GDGCHC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 86
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 394
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 376, 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 3 cut(s) 135, 227, 233
NciI CCSGG 1 cut(s) 376
NdeII GATC 2 cut(s) 480, 543
NlaIV GGNNCC 1 cut(s) 373
NmeAIII GCCGAG 1 cut(s) 193
PfeI GAWTC 2 cut(s) 77, 338
PkrI GCNGC 3 cut(s) 137, 229, 424
Ple19I CGATCG 1 cut(s) 546
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 2 cut(s) 424, 476
PstI CTGCAG 1 cut(s) 118
PvuI CGATCG 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 394
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
RseI CAYNNNNRTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 3 cut(s) 136, 228, 423
Sau3AI GATC 2 cut(s) 480, 543
Sau96I GGNCC 2 cut(s) 424, 476
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 376, 503
SduI GDGCHC 1 cut(s) 162
SfcI CTRYAG 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 236
SsiI CCGC 4 cut(s) 127, 422, 437, 575
SspMI CTAG 2 cut(s) 245, 561
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 374, 501
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 4 cut(s) 362, 405, 446, 468
TaqI TCGA 2 cut(s) 75, 324
TauI GCSGC 1 cut(s) 425
TfiI GAWTC 2 cut(s) 77, 338
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TseI GCWGC 2 cut(s) 135, 227
TspDTI ATGAA 3 cut(s) 17, 112, 368
TspGWI ACGGA 5 cut(s) 7, 47, 446, 464, 503
XspI CTAG 2 cut(s) 245, 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.