RchiOBHm_Chr4g0443491

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004993
Physical Location & Seq
Forward (+)
64600765 .. 64602197
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41119

Sequence Viewer

Length: 612 bp
ATGAAAAACCATCTCCCTCTCACCTCACTCTCACTCCTCATCTTCCTCTTCCTCCTCCGCTCACCCCCAGCCGTCGGATTCCCCTCTCCCGCTCCCTCCACCAACGACTCAGATTTCATCCGCACCAGCTGCGGCACCACCCTCTACCCCGAGCTCTGCTACTCCTCCCTCTCCCGCTACGCCAACGCCGTCCAGAGCAACCCTTCTCAGCTCGCCAAGGTCGCCATCGGCGTCAGCCTCTCCAACGCCAAGCGCATGGCCGCCTTCGTCTCCAACCTCTCCCGCCACGCCGACTACGGCTCCGACCACCGCGCCACCGCCGCCCTCCACGACTGCTTCAGCAACTTCGACGACGCCGTCGATCAGATTCGCGACTCACTCCGGCAGATGAAGAGCCTCGGTTCGGCTCCTGGGTCGGTCCGGTTTCAGATGAGCAACGTCCAGACGTGGATGAGCGCCGCTCTGACGGACCAGGATACGTGTACGGACGGGTTTCAGGACGTGCCGGACGGGTCGGTCAAGTCCGACGTGGTGAACAAGGTCGAGAAAGTGAAGAAGGTCACGAGTAATGCCCTCGCGCTCGTCAACAGAGTGGCGGAGAAAGGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

203

Amino Acids

21.86

Weight (kDa)

8.36

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 194 2.7e-45 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 356, 515
AccB1I GGYRCC 1 cut(s) 134
AccBSI CCGCTC 3 cut(s) 60, 92, 461
AccII CGCG 3 cut(s) 312, 372, 578
AcoI YGGCCR 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 322
AcyI GRCGYC 2 cut(s) 231, 354
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 2 cut(s) 74, 403
AflIII ACRYGT 1 cut(s) 479
AjiI CACGTC 3 cut(s) 447, 502, 529
AjnI CCWGG 2 cut(s) 409, 471
AluBI AGCT 3 cut(s) 129, 154, 211
AluI AGCT 3 cut(s) 129, 154, 211
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 1 cut(s) 129
AspLEI GCGC 4 cut(s) 255, 314, 458, 580
AspS9I GGNCC 2 cut(s) 418, 469
AsuHPI GGTGA 3 cut(s) 13, 54, 544
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 2 cut(s) 418, 469
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 156
BauI CACGAG 1 cut(s) 562
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 116
BccI CCATC 2 cut(s) 18, 233
BceAI ACGGC 4 cut(s) 56, 173, 313, 341
BciT130I CCWGG 2 cut(s) 411, 473
BciVI GTATCC 1 cut(s) 469
BcoDI GTCTC 1 cut(s) 274
BfoI RGCGCY 1 cut(s) 459
BfuI GTATCC 1 cut(s) 469
BisI GCNGC 5 cut(s) 130, 133, 261, 321, 459
BlsI GCNGC 5 cut(s) 131, 134, 262, 322, 460
Bme1390I CCNGG 2 cut(s) 411, 473
Bme18I GGWCC 2 cut(s) 418, 469
BmeT110I CYCGRG 1 cut(s) 149
BmgBI CACGTC 3 cut(s) 447, 502, 529
BmgT120I GGNCC 2 cut(s) 418, 469
BmiI GGNNCC 3 cut(s) 136, 301, 408
BmrFI CCNGG 2 cut(s) 411, 473
BplI GAGNNNNNCTC 2 cut(s) 445, 477
BsaAI YACGTR 1 cut(s) 480
BsaHI GRCGYC 2 cut(s) 231, 354
BsaJI CCNNGG 3 cut(s) 216, 397, 410
BsaWI WCCGGW 1 cut(s) 420
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 284, 314
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 403
BseBI CCWGG 2 cut(s) 411, 473
BseDI CCNNGG 3 cut(s) 216, 397, 410
BseGI GGATG 2 cut(s) 117, 456
BseLI CCNNNNNNNGG 2 cut(s) 74, 403
BseMII CTCAG 2 cut(s) 123, 221
BseRI GAGGAG 3 cut(s) 26, 44, 154
BseXI GCAGC 1 cut(s) 116
BseYI CCCAGC 1 cut(s) 67
Bsh1236I CGCG 3 cut(s) 312, 372, 578
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 3 cut(s) 382, 421, 506
BslI CCNNNNNNNGG 2 cut(s) 74, 403
BsmAI GTCTC 1 cut(s) 274
BsmBI CGTCTC 1 cut(s) 274
BsnI GGCC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 1 cut(s) 361
Bsp68I TCGCGA 1 cut(s) 372
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 2 cut(s) 122, 220
BspFNI CGCG 3 cut(s) 312, 372, 578
BspLI GGNNCC 3 cut(s) 136, 301, 408
BspQI GCTCTTC 1 cut(s) 386
BspT107I GGYRCC 1 cut(s) 134
BsrBI CCGCTC 3 cut(s) 60, 92, 461
BssECI CCNNGG 3 cut(s) 216, 397, 410
BssMI GATC 1 cut(s) 361
BssNI GRCGYC 2 cut(s) 231, 354
BssSI CACGAG 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 216
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 2 cut(s) 411, 473
Bst6I CTCTTC 2 cut(s) 53, 386
BstACI GRCGYC 2 cut(s) 231, 354
BstAPI GCANNNNNTGC 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 2 cut(s) 109, 207
BstF5I GGATG 2 cut(s) 117, 456
BstFNI CGCG 3 cut(s) 312, 372, 578
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 4 cut(s) 255, 314, 458, 580
BstKTI GATC 1 cut(s) 364
BstMAI GTCTC 1 cut(s) 274
BstMBI GATC 1 cut(s) 361
BstMWI GCNNNNNNNGC 3 cut(s) 129, 221, 320
BstNI CCWGG 2 cut(s) 411, 473
BstSCI CCNGG 2 cut(s) 409, 471
BstUI CGCG 3 cut(s) 312, 372, 578
BstV1I GCAGC 1 cut(s) 116
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 1 cut(s) 260
BtrI CACGTC 3 cut(s) 447, 502, 529
BtsCI GGATG 2 cut(s) 117, 456
BtuMI TCGCGA 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 213
CfoI GCGC 4 cut(s) 255, 314, 458, 580
Cfr13I GGNCC 2 cut(s) 418, 469
CpoI CGGWCCG 1 cut(s) 418
CseI GACGC 2 cut(s) 220, 362
Csp6I GTAC 1 cut(s) 483
CspI CGGWCCG 1 cut(s) 418
CviAII CATG 1 cut(s) 256
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 2 cut(s) 109, 207
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
DrdI GACNNNNNNGTC 2 cut(s) 356, 515
DseDI GACNNNNNNGTC 2 cut(s) 356, 515
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 53, 386
EarI CTCTTC 2 cut(s) 53, 386
EciI GGCGGA 1 cut(s) 611
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 418, 469
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 149
EcoICRI GAGCTC 1 cut(s) 154
EcoRII CCWGG 2 cut(s) 409, 471
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 1 cut(s) 216
Esp3I CGTCTC 1 cut(s) 274
FaeI CATG 1 cut(s) 259
FaiI YATR 1 cut(s) 257
FatI CATG 1 cut(s) 255
FauI CCCGC 3 cut(s) 97, 182, 290
Fnu4HI GCNGC 5 cut(s) 130, 133, 261, 321, 459
FokI GGATG 2 cut(s) 104, 463
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 5 cut(s) 130, 133, 261, 321, 459
GlaI GCGC 4 cut(s) 254, 313, 457, 579
GluI GCNGC 5 cut(s) 130, 133, 261, 321, 459
GsaI CCCAGC 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 1 cut(s) 260
HapII CCGG 3 cut(s) 382, 421, 506
HgaI GACGC 2 cut(s) 220, 362
HhaI GCGC 4 cut(s) 255, 314, 458, 580
Hin1I GRCGYC 2 cut(s) 231, 354
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 4 cut(s) 253, 312, 456, 578
HinP1I GCGC 4 cut(s) 253, 312, 456, 578
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HinfI GANTC 4 cut(s) 78, 107, 367, 374
HpaII CCGG 3 cut(s) 382, 421, 506
HphI GGTGA 3 cut(s) 13, 54, 544
Hpy166II GTNNAC 3 cut(s) 483, 535, 586
Hpy188I TCNGA 7 cut(s) 77, 112, 304, 366, 429, 465, 526
Hpy188III TCNNGA 6 cut(s) 193, 371, 442, 497, 544, 562
Hpy8I GTNNAC 3 cut(s) 483, 535, 586
Hpy99I CGWCG 5 cut(s) 77, 353, 356, 362, 530
HpyAV CCTTC 3 cut(s) 213, 274, 550
HpyCH4IV ACGT 5 cut(s) 438, 446, 479, 501, 528
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 221, 320
HpyF3I CTNAG 2 cut(s) 109, 207
HpySE526I ACGT 5 cut(s) 438, 446, 479, 501, 528
Hsp92I GRCGYC 2 cut(s) 231, 354
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 4 cut(s) 253, 312, 456, 578
Kzo9I GATC 1 cut(s) 361
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 3 cut(s) 97, 305, 412
Lsp1109I GCAGC 1 cut(s) 116
MaeII ACGT 5 cut(s) 438, 446, 479, 501, 528
MaeIII GTNAC 1 cut(s) 559
MalI GATC 1 cut(s) 363
MbiI CCGCTC 3 cut(s) 60, 92, 461
MboI GATC 1 cut(s) 361
MboII GAAGA 4 cut(s) 34, 40, 403, 565
MhlI GDGCHC 1 cut(s) 156
MlyI GAGTC 2 cut(s) 101, 368
MmeI TCCRAC 5 cut(s) 55, 267, 297, 327, 549
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 3 cut(s) 382, 421, 506
MspR9I CCNGG 2 cut(s) 411, 473
MvaI CCWGG 2 cut(s) 411, 473
MvnI CGCG 3 cut(s) 312, 372, 578
MwoI GCNNNNNNNGC 3 cut(s) 129, 221, 320
NdeII GATC 1 cut(s) 361
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 3 cut(s) 136, 301, 408
NmuCI GTSAC 1 cut(s) 559
NruI TCGCGA 1 cut(s) 372
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 228, 354, 357
PfeI GAWTC 2 cut(s) 78, 367
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 5 cut(s) 131, 134, 262, 322, 460
PleI GAGTC 2 cut(s) 101, 368
PpsI GAGTC 2 cut(s) 101, 368
Ppu21I YACGTR 1 cut(s) 480
Psp124BI GAGCTC 1 cut(s) 156
Psp6I CCWGG 2 cut(s) 409, 471
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 2 cut(s) 409, 471
PspN4I GGNNCC 3 cut(s) 136, 301, 408
PspPI GGNCC 2 cut(s) 418, 469
PsyI GACNNNGTC 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 129
RruI TCGCGA 1 cut(s) 372
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
Rsr2I CGGWCCG 1 cut(s) 418
RsrII CGGWCCG 1 cut(s) 418
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 386
SatI GCNGC 5 cut(s) 130, 133, 261, 321, 459
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 2 cut(s) 418, 469
SchI GAGTC 2 cut(s) 101, 368
ScrFI CCNGG 2 cut(s) 411, 473
SduI GDGCHC 1 cut(s) 156
SinI GGWCC 2 cut(s) 418, 469
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 409, 471
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 5 cut(s) 441, 449, 482, 504, 531
TaqI TCGA 3 cut(s) 348, 360, 543
TaqII GACCGA 2 cut(s) 406, 505
TauI GCSGC 4 cut(s) 135, 263, 323, 461
TfiI GAWTC 2 cut(s) 78, 367
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 17, 106, 404
TspGWI ACGGA 2 cut(s) 482, 500
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 418, 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.