MD16G1021700.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004993
Physical Location & Seq
Forward (+)
1539138 .. 1539515
378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1021700.v1.1.491

Sequence Viewer

Length: 378 bp
ATGGCTGCCTACTTCTCCAACATATCCCGTCAAGCTGACTACGGCGCCCACCCTCGAGCCGCCTCAGCCCTCCACGACTGCTTCTCCAACTTCGGCGACGCAGTCGACGAGATTCGAGGATCCCTCAAGCAGATGCGCCAGCTCAGTTCCACTGCAAACTCAAGAGGCGGATCCTCGTCATTCCGGTTCCAGATGAGCAACGTCCAGACGTGGATGAGCGCCGCGCTTACCGACGAGGAGACCTGTACGGACGGGTTTGATGACGTGGAGAATGGGCCCCTCAAGACCGACGTGTCGAACCGGGTTGAGAATGTGAAGAAGGTTACAAGCAATGCCCTTGCGCTCGTTAATAGCGTTGCTGAGAAGGGCGCGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

126

Amino Acids

13.46

Weight (kDa)

5.26

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 3 - 115 1.6e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 292
AccB1I GGYRCC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 105
AccII CGCG 2 cut(s) 224, 371
AciI CCGC 3 cut(s) 60, 168, 222
AclWI GGATC 4 cut(s) 114, 127, 165, 178
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 1 cut(s) 247
AflIII ACRYGT 1 cut(s) 291
AjiI CACGTC 3 cut(s) 210, 265, 292
AluBI AGCT 2 cut(s) 35, 142
AluI AGCT 2 cut(s) 35, 142
Alw26I GTCTC 1 cut(s) 233
AlwI GGATC 4 cut(s) 114, 127, 165, 178
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 1 cut(s) 275
ApaI GGGCCC 1 cut(s) 279
ApeKI GCWGC 1 cut(s) 5
AscI GGCGCGCC 1 cut(s) 369
AspLEI GCGC 7 cut(s) 47, 138, 221, 226, 343, 371, 373
AspS9I GGNCC 2 cut(s) 275, 276
AsuC2I CCSGG 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 279
BamHI GGATCC 2 cut(s) 119, 170
BanI GGYRCC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 64
BceAI ACGGC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 233
BfoI RGCGCY 2 cut(s) 48, 222
BisI GCNGC 3 cut(s) 6, 60, 222
BlsI GCNGC 3 cut(s) 7, 61, 223
Bme1390I CCNGG 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 54
BmgBI CACGTC 3 cut(s) 210, 265, 292
BmgT120I GGNCC 2 cut(s) 275, 276
BmiI GGNNCC 6 cut(s) 46, 121, 172, 188, 277, 278
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 108, 140
Bpu10I CCTNAGC 1 cut(s) 64
BpuEI CTTGAG 3 cut(s) 110, 145, 266
BpuMI CCSGG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 1 cut(s) 233
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse3DI GCAATG 1 cut(s) 337
BseGI GGATG 1 cut(s) 219
BseMI GCAATG 1 cut(s) 337
BseMII CTCAG 3 cut(s) 78, 157, 351
BsePI GCGCGC 1 cut(s) 369
BseRI GAGGAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 279
Bsh1236I CGCG 2 cut(s) 224, 371
BshFI GGCC 1 cut(s) 277
BshNI GGYRCC 1 cut(s) 44
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 2 cut(s) 184, 301
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 1 cut(s) 277
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 1 cut(s) 54
Bsp120I GGGCCC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 119, 170
BspACI CCGC 3 cut(s) 60, 168, 222
BspANI GGCC 1 cut(s) 277
BspCNI CTCAG 3 cut(s) 77, 156, 352
BspFNI CGCG 2 cut(s) 224, 371
BspLI GGNNCC 6 cut(s) 46, 121, 172, 188, 277, 278
BspPI GGATC 4 cut(s) 114, 127, 165, 178
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 337
BssHII GCGCGC 1 cut(s) 369
BssMI GATC 2 cut(s) 119, 170
BssNI GRCGYC 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 140, 371
BstDEI CTNAG 3 cut(s) 64, 143, 360
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 2 cut(s) 224, 371
BstH2I RGCGCY 2 cut(s) 48, 222
BstHHI GCGC 7 cut(s) 47, 138, 221, 226, 343, 371, 373
BstKTI GATC 2 cut(s) 122, 173
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 2 cut(s) 119, 170
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 2 cut(s) 224, 371
BstX2I RGATCY 2 cut(s) 119, 170
BstYI RGATCY 2 cut(s) 119, 170
BsuRI GGCC 1 cut(s) 277
BtrI CACGTC 3 cut(s) 210, 265, 292
BtsCI GGATG 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 140, 371
CfoI GCGC 7 cut(s) 47, 138, 221, 226, 343, 371, 373
Cfr13I GGNCC 2 cut(s) 275, 276
CseI GACGC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 246
CviJI RGCY 6 cut(s) 5, 35, 59, 68, 142, 277
CviKI_1 RGCY 6 cut(s) 5, 35, 59, 68, 142, 277
CviQI GTAC 1 cut(s) 246
DdeI CTNAG 3 cut(s) 64, 143, 360
DinI GGCGCC 1 cut(s) 46
DpnI GATC 2 cut(s) 121, 172
DpnII GATC 2 cut(s) 119, 170
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
EciI GGCGGA 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 233
Eco88I CYCGRG 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 279
EgeI GGCGCC 1 cut(s) 46
EheI GGCGCC 1 cut(s) 46
FaiI YATR 1 cut(s) 23
FblI GTMKAC 1 cut(s) 105
Fnu4HI GCNGC 3 cut(s) 6, 60, 222
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 3 cut(s) 6, 60, 222
GlaI GCGC 7 cut(s) 46, 137, 220, 225, 342, 370, 372
GluI GCNGC 3 cut(s) 6, 60, 222
HaeII RGCGCY 2 cut(s) 48, 222
HaeIII GGCC 1 cut(s) 277
HapII CCGG 2 cut(s) 184, 301
HgaI GACGC 1 cut(s) 107
HhaI GCGC 7 cut(s) 47, 138, 221, 226, 343, 371, 373
Hin1I GRCGYC 1 cut(s) 45
Hin6I GCGC 7 cut(s) 45, 136, 219, 224, 341, 369, 371
HinP1I GCGC 7 cut(s) 45, 136, 219, 224, 341, 369, 371
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 1 cut(s) 112
HpaII CCGG 2 cut(s) 184, 301
Hpy166II GTNNAC 1 cut(s) 106
Hpy188III TCNNGA 4 cut(s) 162, 190, 205, 283
Hpy8I GTNNAC 1 cut(s) 106
Hpy99I CGWCG 4 cut(s) 101, 110, 236, 293
HpyAV CCTTC 2 cut(s) 313, 358
HpyCH4IV ACGT 4 cut(s) 201, 209, 264, 291
HpyCH4V TGCA 1 cut(s) 155
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 3 cut(s) 64, 143, 360
HpySE526I ACGT 4 cut(s) 201, 209, 264, 291
Hsp92I GRCGYC 1 cut(s) 45
HspAI GCGC 7 cut(s) 45, 136, 219, 224, 341, 369, 371
KasI GGCGCC 1 cut(s) 44
Kzo9I GATC 2 cut(s) 119, 170
LpnPI CCDG 6 cut(s) 152, 197, 203, 218, 256, 314
LweI GCATC 1 cut(s) 123
MaeII ACGT 4 cut(s) 201, 209, 264, 291
MaeIII GTNAC 1 cut(s) 322
MalI GATC 2 cut(s) 121, 172
MboI GATC 2 cut(s) 119, 170
MboII GAAGA 1 cut(s) 328
MflI RGATCY 2 cut(s) 119, 170
MhlI GDGCHC 1 cut(s) 279
Mly113I GGCGCC 1 cut(s) 45
MmeI TCCRAC 2 cut(s) 42, 111
MnlI CCTC 9 cut(s) 63, 73, 80, 110, 134, 158, 184, 229, 290
MseI TTAA 1 cut(s) 348
MspI CCGG 2 cut(s) 184, 301
MspR9I CCNGG 1 cut(s) 302
MteI GCGCNGCGC 1 cut(s) 222
MvnI CGCG 2 cut(s) 224, 371
MwoI GCNNNNNNNGC 1 cut(s) 65
NarI GGCGCC 1 cut(s) 45
NciI CCSGG 1 cut(s) 302
NdeII GATC 2 cut(s) 119, 170
NlaIV GGNNCC 6 cut(s) 46, 121, 172, 188, 277, 278
PaeR7I CTCGAG 1 cut(s) 54
PalAI GGCGCGCC 1 cut(s) 369
PauI GCGCGC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 351
PfeI GAWTC 1 cut(s) 112
PflFI GACNNNGTC 1 cut(s) 101
PkrI GCNGC 3 cut(s) 7, 61, 223
PluTI GGCGCC 1 cut(s) 48
PspN4I GGNNCC 6 cut(s) 46, 121, 172, 188, 277, 278
PspOMI GGGCCC 1 cut(s) 275
PspPI GGNCC 2 cut(s) 275, 276
PspXI VCTCGAGB 1 cut(s) 54
PsuI RGATCY 2 cut(s) 119, 170
PsyI GACNNNGTC 1 cut(s) 101
PteI GCGCGC 1 cut(s) 369
RsaI GTAC 1 cut(s) 247
RsaNI GTAC 1 cut(s) 246
SalI GTCGAC 1 cut(s) 104
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 6, 60, 222
Sau3AI GATC 2 cut(s) 119, 170
Sau96I GGNCC 2 cut(s) 275, 276
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 279
SetI ASST 8 cut(s) 37, 144, 204, 212, 245, 267, 294, 324
SfaNI GCATC 1 cut(s) 123
SfoI GGCGCC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 54
SgsI GGCGCGCC 1 cut(s) 369
SlaI CTCGAG 1 cut(s) 54
SmlI CTYRAG 4 cut(s) 54, 125, 160, 281
SmoI CTYRAG 4 cut(s) 54, 125, 160, 281
SsiI CCGC 3 cut(s) 60, 168, 222
SspDI GGCGCC 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 300
TaiI ACGT 4 cut(s) 204, 212, 267, 294
TaqI TCGA 4 cut(s) 55, 105, 115, 296
TaqII GACCGA 1 cut(s) 302
TauI GCSGC 2 cut(s) 62, 224
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 157
TseI GCWGC 1 cut(s) 5
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 157
Tth111I GACNNNGTC 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 54
XmiI GTMKAC 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.