MD00G1133700.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004993
Physical Location & Seq
Forward (+)
28985633 .. 28986268
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1133700.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAACACCAAATGCATGTCCACCCACTTCCTCCTCCCCCAACTCTTCCTCACCTTCCTCTTCTTCCTCCTCTTGCACCCAATCCCCACCATCGGATCCCCATCCAACGACGCGGAATTCATCCGTACAAGCTGCGGCGTCACTCTGTACCCTGACCTCTGCTACACCTCCCTTTCCCCCTTCGCCAGCGCCGTCCAAGACAACCCGGCTCAGCTCGCCAAGGTCGCCATCGGCGTCAGCCTCGCCCGGGCACGCAGCATGGCCGCCTACGTCTCCAACATCTCACGTCAAGCCGACTACGGTGCCGACTCGCGCGCCGCCTCCGCCCTCCACGACTGCTTCTCCAACTTCGGCGACGCTGTCGACGAGATTCGTGGCTCCCTCAAGCAGATGCGCCAGCTCATTTCCACCGCAGCCTCCGGTGGTGGGTCGTCGTCGTTCCGGTTCCAGATGAGCAACGTCCAGACGTGGATGAGCGCCGCGCTTACCGACGAGGAGACCTGTACGGACGGGTTTGATGACGTGGAGGATGGCCCTCTCAAGACCGACGTGTCGAACCGGGTGGAGAATGTGAAGAAGGTTACCAGCAATGCCCTCGCTCTCGTTAATAGGGTTGCTGAGAAGGGCGCGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

212

Amino Acids

22.69

Weight (kDa)

5.32

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 201 1.6e-43 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 550
AccB1I GGYRCC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 363
AccII CGCG 5 cut(s) 113, 313, 315, 482, 629
AciI CCGC 7 cut(s) 113, 135, 264, 318, 324, 411, 480
AclWI GGATC 2 cut(s) 90, 103
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 1 cut(s) 116
AcyI GRCGYC 2 cut(s) 138, 234
AfaI GTAC 3 cut(s) 127, 149, 505
AfiI CCNNNNNNNGG 3 cut(s) 92, 247, 426
AflIII ACRYGT 1 cut(s) 549
AjiI CACGTC 4 cut(s) 287, 468, 523, 550
AluBI AGCT 3 cut(s) 132, 214, 400
AluI AGCT 3 cut(s) 132, 214, 400
Alw26I GTCTC 2 cut(s) 277, 491
AlwI GGATC 2 cut(s) 90, 103
Ama87I CYCGRG 1 cut(s) 246
AoxI GGCC 2 cut(s) 261, 532
ApeKI GCWGC 3 cut(s) 132, 255, 413
ApoI RAATTY 1 cut(s) 116
AscI GGCGCGCC 1 cut(s) 627
AspLEI GCGC 8 cut(s) 191, 315, 317, 396, 479, 484, 629, 631
AspS9I GGNCC 1 cut(s) 533
AsuC2I CCSGG 4 cut(s) 206, 247, 248, 560
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 246
BaeGI GKGCMC 1 cut(s) 253
BamHI GGATCC 1 cut(s) 95
BanI GGYRCC 1 cut(s) 302
BbvI GCAGC 3 cut(s) 119, 267, 425
BccI CCATC 4 cut(s) 98, 109, 236, 524
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 4 cut(s) 284, 318, 577, 611
BcnI CCSGG 4 cut(s) 206, 247, 248, 560
BcoDI GTCTC 2 cut(s) 277, 491
BfoI RGCGCY 2 cut(s) 192, 480
BisI GCNGC 7 cut(s) 133, 136, 256, 264, 318, 414, 480
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 7 cut(s) 134, 137, 257, 265, 319, 415, 481
Bme1390I CCNGG 4 cut(s) 206, 247, 248, 560
BmeT110I CYCGRG 1 cut(s) 246
BmgBI CACGTC 4 cut(s) 287, 468, 523, 550
BmgT120I GGNCC 1 cut(s) 533
BmiI GGNNCC 4 cut(s) 97, 304, 379, 446
BmrFI CCNGG 4 cut(s) 206, 247, 248, 560
BmsI GCATC 1 cut(s) 381
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 368, 524
BpuMI CCSGG 4 cut(s) 206, 247, 248, 560
BsaBI GATNNNNATC 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 138, 234
BsaI GGTCTC 1 cut(s) 491
BsaJI CCNNGG 2 cut(s) 219, 246
BsaWI WCCGGW 2 cut(s) 419, 441
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 247, 426
Bse3DI GCAATG 1 cut(s) 595
Bse8I GATNNNNATC 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 219, 246
BseGI GGATG 4 cut(s) 101, 120, 477, 535
BseJI GATNNNNATC 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 92, 247, 426
BseMI GCAATG 1 cut(s) 595
BseMII CTCAG 2 cut(s) 224, 609
BsePI GCGCGC 2 cut(s) 313, 627
BseRI GAGGAG 3 cut(s) 23, 59, 509
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 3 cut(s) 119, 267, 425
Bsh1236I CGCG 5 cut(s) 113, 313, 315, 482, 629
BshFI GGCC 2 cut(s) 263, 534
BshNI GGYRCC 1 cut(s) 302
BsiHKCI CYCGRG 1 cut(s) 246
BsiSI CCGG 5 cut(s) 206, 247, 420, 442, 559
BslI CCNNNNNNNGG 3 cut(s) 92, 247, 426
BsmAI GTCTC 2 cut(s) 277, 491
BsmBI CGTCTC 1 cut(s) 277
BsnI GGCC 2 cut(s) 263, 534
Bso31I GGTCTC 1 cut(s) 491
BsoBI CYCGRG 1 cut(s) 246
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 1 cut(s) 95
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 7 cut(s) 113, 135, 264, 318, 324, 411, 480
BspANI GGCC 2 cut(s) 263, 534
BspCNI CTCAG 2 cut(s) 223, 610
BspFNI CGCG 5 cut(s) 113, 313, 315, 482, 629
BspLI GGNNCC 4 cut(s) 97, 304, 379, 446
BspPI GGATC 2 cut(s) 90, 103
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 595
BssECI CCNNGG 2 cut(s) 219, 246
BssHII GCGCGC 2 cut(s) 313, 627
BssMI GATC 1 cut(s) 95
BssNI GRCGYC 2 cut(s) 138, 234
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 2 cut(s) 50, 65
BstACI GRCGYC 2 cut(s) 138, 234
BstC8I GCNNGC 6 cut(s) 187, 216, 253, 315, 398, 629
BstDEI CTNAG 2 cut(s) 210, 618
BstEII GGTNACC 1 cut(s) 580
BstF5I GGATG 4 cut(s) 101, 120, 477, 535
BstFNI CGCG 5 cut(s) 113, 313, 315, 482, 629
BstH2I RGCGCY 2 cut(s) 192, 480
BstHHI GCGC 8 cut(s) 191, 315, 317, 396, 479, 484, 629, 631
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 2 cut(s) 277, 491
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 3 cut(s) 215, 224, 323
BstNSI RCATGY 1 cut(s) 19
BstPI GGTNACC 1 cut(s) 580
BstSCI CCNGG 4 cut(s) 204, 245, 246, 558
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 5 cut(s) 113, 313, 315, 482, 629
BstV1I GCAGC 3 cut(s) 119, 267, 425
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 263, 534
BtrI CACGTC 4 cut(s) 287, 468, 523, 550
BtsCI GGATG 4 cut(s) 101, 120, 477, 535
Cac8I GCNNGC 6 cut(s) 187, 216, 253, 315, 398, 629
CfoI GCGC 8 cut(s) 191, 315, 317, 396, 479, 484, 629, 631
Cfr13I GGNCC 1 cut(s) 533
Cfr9I CCCGGG 1 cut(s) 246
CseI GACGC 4 cut(s) 119, 127, 223, 365
Csp6I GTAC 3 cut(s) 126, 148, 504
CviAII CATG 2 cut(s) 16, 259
CviQI GTAC 3 cut(s) 126, 148, 504
DdeI CTNAG 2 cut(s) 210, 618
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
DrdI GACNNNNNNGTC 1 cut(s) 550
DseDI GACNNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 2 cut(s) 50, 65
EarI CTCTTC 2 cut(s) 50, 65
EciI GGCGGA 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 219
Eco31I GGTCTC 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 246
Eco91I GGTNACC 1 cut(s) 580
EcoO65I GGTNACC 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 116
EcoT14I CCWWGG 1 cut(s) 219
EcoT22I ATGCAT 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 2 cut(s) 19, 262
FaiI YATR 2 cut(s) 17, 260
FatI CATG 2 cut(s) 15, 258
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 7 cut(s) 133, 136, 256, 264, 318, 414, 480
FokI GGATG 4 cut(s) 88, 107, 484, 542
Fsp4HI GCNGC 7 cut(s) 133, 136, 256, 264, 318, 414, 480
GlaI GCGC 8 cut(s) 190, 314, 316, 395, 478, 483, 628, 630
GluI GCNGC 7 cut(s) 133, 136, 256, 264, 318, 414, 480
HaeII RGCGCY 2 cut(s) 192, 480
HaeIII GGCC 2 cut(s) 263, 534
HapII CCGG 5 cut(s) 206, 247, 420, 442, 559
HgaI GACGC 4 cut(s) 119, 127, 223, 365
HhaI GCGC 8 cut(s) 191, 315, 317, 396, 479, 484, 629, 631
Hin1I GRCGYC 2 cut(s) 138, 234
Hin1II CATG 2 cut(s) 19, 262
Hin6I GCGC 8 cut(s) 189, 313, 315, 394, 477, 482, 627, 629
HinP1I GCGC 8 cut(s) 189, 313, 315, 394, 477, 482, 627, 629
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 2 cut(s) 308, 370
HpaII CCGG 5 cut(s) 206, 247, 420, 442, 559
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 2 cut(s) 21, 364
Hpy188I TCNGA 1 cut(s) 95
Hpy188III TCNNGA 3 cut(s) 448, 463, 541
Hpy8I GTNNAC 2 cut(s) 21, 364
Hpy99I CGWCG 7 cut(s) 113, 359, 368, 436, 439, 494, 551
HpyAV CCTTC 4 cut(s) 64, 190, 571, 616
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 6 cut(s) 270, 286, 459, 467, 522, 549
HpyCH4V TGCA 2 cut(s) 15, 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 224, 323
HpyF3I CTNAG 2 cut(s) 210, 618
HpySE526I ACGT 6 cut(s) 270, 286, 459, 467, 522, 549
Hsp92I GRCGYC 2 cut(s) 138, 234
Hsp92II CATG 2 cut(s) 19, 262
HspAI GCGC 8 cut(s) 189, 313, 315, 394, 477, 482, 627, 629
Kzo9I GATC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 383
Lsp1109I GCAGC 3 cut(s) 119, 267, 425
LweI GCATC 1 cut(s) 381
MaeII ACGT 6 cut(s) 270, 286, 459, 467, 522, 549
MaeIII GTNAC 2 cut(s) 139, 580
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 4 cut(s) 37, 52, 55, 586
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 1 cut(s) 116
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 3 cut(s) 129, 300, 369
Mph1103I ATGCAT 1 cut(s) 17
MseI TTAA 1 cut(s) 606
MspI CCGG 5 cut(s) 206, 247, 420, 442, 559
MspR9I CCNGG 4 cut(s) 206, 247, 248, 560
MteI GCGCNGCGC 1 cut(s) 480
MvnI CGCG 5 cut(s) 113, 313, 315, 482, 629
MwoI GCNNNNNNNGC 3 cut(s) 215, 224, 323
NciI CCSGG 4 cut(s) 206, 247, 248, 560
NdeII GATC 1 cut(s) 95
NlaIII CATG 2 cut(s) 19, 262
NlaIV GGNNCC 4 cut(s) 97, 304, 379, 446
NmuCI GTSAC 1 cut(s) 139
NsiI ATGCAT 1 cut(s) 17
NspI RCATGY 1 cut(s) 19
PalAI GGCGCGCC 1 cut(s) 627
PauI GCGCGC 2 cut(s) 313, 627
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 231, 363
PfeI GAWTC 1 cut(s) 370
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 7 cut(s) 134, 137, 257, 265, 319, 415, 481
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 580
PspN4I GGNNCC 4 cut(s) 97, 304, 379, 446
PspPI GGNCC 1 cut(s) 533
PsuI RGATCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 359
PteI GCGCGC 2 cut(s) 313, 627
RsaI GTAC 3 cut(s) 127, 149, 505
RsaNI GTAC 3 cut(s) 126, 148, 504
SalI GTCGAC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 7 cut(s) 133, 136, 256, 264, 318, 414, 480
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 4 cut(s) 206, 247, 248, 560
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 1 cut(s) 381
SgsI GGCGCGCC 1 cut(s) 627
SmaI CCCGGG 1 cut(s) 248
SmlI CTYRAG 2 cut(s) 383, 539
SmoI CTYRAG 2 cut(s) 383, 539
SrfI GCCCGGGC 1 cut(s) 248
Sse9I AATT 1 cut(s) 116
SsiI CCGC 7 cut(s) 113, 135, 264, 318, 324, 411, 480
StyD4I CCNGG 4 cut(s) 204, 245, 246, 558
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 6 cut(s) 273, 289, 462, 470, 525, 552
TaqI TCGA 2 cut(s) 363, 554
TaqII GACCGA 1 cut(s) 560
TasI AATT 1 cut(s) 116
TauI GCSGC 4 cut(s) 138, 266, 320, 482
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 3 cut(s) 132, 255, 413
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 17, 109
TspGWI ACGGA 2 cut(s) 113, 521
TspMI CCCGGG 1 cut(s) 246
Tth111I GACNNNGTC 1 cut(s) 359
XapI RAATTY 1 cut(s) 116
XceI RCATGY 1 cut(s) 19
XmaI CCCGGG 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 363
Zsp2I ATGCAT 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.