MD00G1134000.v1.1

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0004993
Physical Location & Seq
Forward (+)
29012179 .. 29012805
627 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1134000.v1.1.491

Sequence Viewer

Length: 627 bp
ATGTACACTACCAAACTCATGACCACCCACTTCCTCCTCTTCCAACTCTCACTCATCTCCCTCCTCTTCCTCTTCCACCCAACACCCACCGGCGGATCCCCGTCCAACGACACAGACTTTATACGTACAAGCTGCGGCACTACGTTGTACCCTGACGTCTGCTATACCTCCCTTTCCCGCTACGCCAGCGCCGTCCAGAACAACTCGGCTCATCTCGCCAAGGTTGCCATCGGCGTCAGCCTCGCCAGGGCCCGGAGCTTAGCCGATTACCTCTCCAACATCTCCCGCCAAGCTGACTACGGCGCCGACCCCCGGGCGCCACCCGCCCTCCACGACTGCTTATCCACCATCGGCGACGCCATAGACCAGATTCGTGACTCGCTCAAGCAGATACGCCAGCTCGGTTCCACCAGCCGCGGGTCGTCGTCGTTACGGTTCCAGATGAGCAACGTGCAGACGTGGATGAGCGCCGCGCTTACGAATGAGGAGACCTGTACGGACGGATTTGATAACGTGGCGGAGGGGCCTTTGAAGACCGACGTGTCGAAACGGGTGGAGGATGTGAAGAAGGTAACCAGCAATGCGCTGGCGTTGGTTAACAGAGTTGCTGAGAAGGGCGCACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

209

Amino Acids

22.54

Weight (kDa)

7.69

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 39 - 198 2.5e-41 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 541
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 2 cut(s) 302, 316
AccII CGCG 2 cut(s) 417, 473
AciI CCGC 9 cut(s) 93, 135, 178, 286, 324, 415, 417, 471, 518
AclWI GGATC 2 cut(s) 90, 103
AcyI GRCGYC 5 cut(s) 156, 234, 303, 317, 357
AfaI GTAC 4 cut(s) 5, 127, 149, 496
AfiI CCNNNNNNNGG 5 cut(s) 92, 247, 252, 312, 417
AflIII ACRYGT 1 cut(s) 540
AgsI TTSAA 1 cut(s) 532
AjiI CACGTC 2 cut(s) 459, 541
AjnI CCWGG 1 cut(s) 245
AluBI AGCT 4 cut(s) 132, 258, 293, 400
AluI AGCT 4 cut(s) 132, 258, 293, 400
Alw26I GTCTC 1 cut(s) 482
AlwI GGATC 2 cut(s) 90, 103
Ama87I CYCGRG 1 cut(s) 312
AoxI GGCC 2 cut(s) 249, 524
ApaI GGGCCC 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 132
AspLEI GCGC 7 cut(s) 191, 305, 319, 470, 475, 586, 620
AspS9I GGNCC 3 cut(s) 249, 250, 524
AsuC2I CCSGG 3 cut(s) 253, 313, 314
AvaI CYCGRG 1 cut(s) 312
BaeGI GKGCMC 1 cut(s) 253
BamHI GGATCC 1 cut(s) 95
BanI GGYRCC 2 cut(s) 302, 316
BanII GRGCYC 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 539
BbvI GCAGC 1 cut(s) 119
BccI CCATC 2 cut(s) 236, 356
BceAI ACGGC 2 cut(s) 176, 316
BciT130I CCWGG 1 cut(s) 247
BcnI CCSGG 3 cut(s) 253, 313, 314
BcoDI GTCTC 1 cut(s) 482
BfoI RGCGCY 4 cut(s) 192, 306, 320, 471
BisI GCNGC 4 cut(s) 133, 136, 415, 471
BlpI GCTNAGC 1 cut(s) 259
BlsI GCNGC 4 cut(s) 134, 137, 416, 472
Bme1390I CCNGG 4 cut(s) 247, 253, 313, 314
BmeT110I CYCGRG 1 cut(s) 312
BmgBI CACGTC 2 cut(s) 459, 541
BmgT120I GGNCC 3 cut(s) 249, 250, 524
BmiI GGNNCC 7 cut(s) 97, 251, 304, 318, 406, 437, 525
BmrFI CCNGG 4 cut(s) 247, 253, 313, 314
BpiI GAAGAC 1 cut(s) 539
Bpu1102I GCTNAGC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 3 cut(s) 253, 313, 314
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 5 cut(s) 156, 234, 303, 317, 357
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 5 cut(s) 219, 246, 311, 312, 415
BsaXI ACNNNNNCTCC 2 cut(s) 312, 342
Bsc4I CCNNNNNNNGG 5 cut(s) 92, 247, 252, 312, 417
Bse118I RCCGGY 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 586
BseBI CCWGG 1 cut(s) 247
BseDI CCNNGG 5 cut(s) 219, 246, 311, 312, 415
BseGI GGATG 2 cut(s) 468, 565
BseLI CCNNNNNNNGG 5 cut(s) 92, 247, 252, 312, 417
BseMI GCAATG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 600
BseRI GAGGAG 3 cut(s) 26, 53, 500
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 1 cut(s) 119
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 2 cut(s) 417, 473
BshFI GGCC 2 cut(s) 251, 526
BshNI GGYRCC 2 cut(s) 302, 316
BsiHKCI CYCGRG 1 cut(s) 312
BsiSI CCGG 3 cut(s) 90, 253, 313
BslI CCNNNNNNNGG 5 cut(s) 92, 247, 252, 312, 417
BsmAI GTCTC 1 cut(s) 482
BsnI GGCC 2 cut(s) 251, 526
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 312
Bsp120I GGGCCC 1 cut(s) 249
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 95
Bsp1720I GCTNAGC 1 cut(s) 259
BspACI CCGC 9 cut(s) 93, 135, 178, 286, 324, 415, 417, 471, 518
BspANI GGCC 2 cut(s) 251, 526
BspCNI CTCAG 1 cut(s) 601
BspFNI CGCG 2 cut(s) 417, 473
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 7 cut(s) 97, 251, 304, 318, 406, 437, 525
BspPI GGATC 2 cut(s) 90, 103
BspT107I GGYRCC 2 cut(s) 302, 316
BspTNI GGTCTC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 586
BsrFI RCCGGY 1 cut(s) 89
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 5 cut(s) 219, 246, 311, 312, 415
BssMI GATC 1 cut(s) 95
BssNI GRCGYC 5 cut(s) 156, 234, 303, 317, 357
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 247
Bst4CI ACNGT 1 cut(s) 435
Bst6I CTCTTC 3 cut(s) 44, 71, 77
BstACI GRCGYC 5 cut(s) 156, 234, 303, 317, 357
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 125
BstC8I GCNNGC 3 cut(s) 187, 398, 588
BstDEI CTNAG 2 cut(s) 259, 609
BstDSI CCRYGG 1 cut(s) 415
BstEII GGTNACC 1 cut(s) 571
BstF5I GGATG 2 cut(s) 468, 565
BstFNI CGCG 2 cut(s) 417, 473
BstH2I RGCGCY 4 cut(s) 192, 306, 320, 471
BstHHI GCGC 7 cut(s) 191, 305, 319, 470, 475, 586, 620
BstKTI GATC 1 cut(s) 98
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 186, 215, 224, 323
BstNI CCWGG 1 cut(s) 247
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 4 cut(s) 245, 251, 311, 312
BstSLI GKGCMC 1 cut(s) 253
BstSNI TACGTA 1 cut(s) 125
BstUI CGCG 2 cut(s) 417, 473
BstV1I GCAGC 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 251, 526
BtgI CCRYGG 1 cut(s) 415
BtrI CACGTC 2 cut(s) 459, 541
BtsCI GGATG 2 cut(s) 468, 565
Cac8I GCNNGC 3 cut(s) 187, 398, 588
CciI TCATGA 1 cut(s) 18
CfoI GCGC 7 cut(s) 191, 305, 319, 470, 475, 586, 620
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 3 cut(s) 249, 250, 524
Cfr42I CCGCGG 1 cut(s) 418
Cfr9I CCCGGG 1 cut(s) 312
CseI GACGC 2 cut(s) 223, 365
Csp6I GTAC 4 cut(s) 4, 126, 148, 495
CviAII CATG 1 cut(s) 19
CviQI GTAC 4 cut(s) 4, 126, 148, 495
DdeI CTNAG 2 cut(s) 259, 609
DinI GGCGCC 2 cut(s) 304, 318
DpnI GATC 1 cut(s) 97
DpnII GATC 1 cut(s) 95
DrdI GACNNNNNNGTC 1 cut(s) 541
DseDI GACNNNNNNGTC 1 cut(s) 541
Eam1104I CTCTTC 3 cut(s) 44, 71, 77
EarI CTCTTC 3 cut(s) 44, 71, 77
EciI GGCGGA 2 cut(s) 108, 533
Eco105I TACGTA 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 312
Eco91I GGTNACC 1 cut(s) 571
EcoO109I RGGNCCY 2 cut(s) 249, 524
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 253
EgeI GGCGCC 2 cut(s) 304, 318
EheI GGCGCC 2 cut(s) 304, 318
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 20, 122, 165, 362
FatI CATG 1 cut(s) 18
FauI CCCGC 4 cut(s) 185, 293, 331, 410
Fnu4HI GCNGC 4 cut(s) 133, 136, 415, 471
FokI GGATG 2 cut(s) 475, 572
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 4 cut(s) 133, 136, 415, 471
GlaI GCGC 7 cut(s) 190, 304, 318, 469, 474, 585, 619
GluI GCNGC 4 cut(s) 133, 136, 415, 471
HaeII RGCGCY 4 cut(s) 192, 306, 320, 471
HaeIII GGCC 2 cut(s) 251, 526
HapII CCGG 3 cut(s) 90, 253, 313
HgaI GACGC 2 cut(s) 223, 365
HhaI GCGC 7 cut(s) 191, 305, 319, 470, 475, 586, 620
Hin1I GRCGYC 5 cut(s) 156, 234, 303, 317, 357
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 7 cut(s) 189, 303, 317, 468, 473, 584, 618
HinP1I GCGC 7 cut(s) 189, 303, 317, 468, 473, 584, 618
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 2 cut(s) 370, 377
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 3 cut(s) 90, 253, 313
Hpy166II GTNNAC 2 cut(s) 6, 598
Hpy188III TCNNGA 4 cut(s) 19, 196, 374, 439
Hpy8I GTNNAC 2 cut(s) 6, 598
Hpy99I CGWCG 4 cut(s) 359, 427, 430, 542
HpyAV CCTTC 2 cut(s) 562, 607
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4IV ACGT 7 cut(s) 124, 143, 156, 450, 458, 513, 540
HpyCH4V TGCA 1 cut(s) 454
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 215, 224, 323
HpyF3I CTNAG 2 cut(s) 259, 609
HpySE526I ACGT 7 cut(s) 124, 143, 156, 450, 458, 513, 540
Hsp92I GRCGYC 5 cut(s) 156, 234, 303, 317, 357
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 7 cut(s) 189, 303, 317, 468, 473, 584, 618
KasI GGCGCC 2 cut(s) 302, 316
KspAI GTTAAC 1 cut(s) 598
KspI CCGCGG 1 cut(s) 418
Kzo9I GATC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 255
Lsp1109I GCAGC 1 cut(s) 119
MaeII ACGT 7 cut(s) 124, 143, 156, 450, 458, 513, 540
MaeIII GTNAC 3 cut(s) 374, 429, 571
MalI GATC 1 cut(s) 97
MboI GATC 1 cut(s) 95
MboII GAAGA 5 cut(s) 31, 58, 64, 544, 577
MflI RGATCY 1 cut(s) 95
MhlI GDGCHC 1 cut(s) 253
Mly113I GGCGCC 2 cut(s) 303, 317
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 3 cut(s) 67, 129, 300
MseI TTAA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 417
MspI CCGG 3 cut(s) 90, 253, 313
MspR9I CCNGG 4 cut(s) 247, 253, 313, 314
MteI GCGCNGCGC 1 cut(s) 471
MvaI CCWGG 1 cut(s) 247
MvnI CGCG 2 cut(s) 417, 473
MwoI GCNNNNNNNGC 4 cut(s) 186, 215, 224, 323
NarI GGCGCC 2 cut(s) 303, 317
NciI CCSGG 3 cut(s) 253, 313, 314
NdeII GATC 1 cut(s) 95
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 7 cut(s) 97, 251, 304, 318, 406, 437, 525
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 374
PagI TCATGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 4 cut(s) 134, 137, 416, 472
PleI GAGTC 1 cut(s) 371
PluTI GGCGCC 2 cut(s) 306, 320
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 125
Psp6I CCWGG 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 245
PspN4I GGNNCC 7 cut(s) 97, 251, 304, 318, 406, 437, 525
PspOMI GGGCCC 1 cut(s) 249
PspPI GGNCC 3 cut(s) 249, 250, 524
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 4 cut(s) 5, 127, 149, 496
RsaNI GTAC 4 cut(s) 4, 126, 148, 495
SacII CCGCGG 1 cut(s) 418
SaqAI TTAA 1 cut(s) 597
SatI GCNGC 4 cut(s) 133, 136, 415, 471
Sau3AI GATC 1 cut(s) 95
Sau96I GGNCC 3 cut(s) 249, 250, 524
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 4 cut(s) 247, 253, 313, 314
SduI GDGCHC 1 cut(s) 253
SfoI GGCGCC 2 cut(s) 304, 318
Sfr303I CCGCGG 1 cut(s) 418
SgrAI CRCCGGYG 1 cut(s) 89
SgrBI CCGCGG 1 cut(s) 418
SmaI CCCGGG 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
SnaBI TACGTA 1 cut(s) 125
SsiI CCGC 9 cut(s) 93, 135, 178, 286, 324, 415, 417, 471, 518
SspDI GGCGCC 2 cut(s) 302, 316
StyD4I CCNGG 4 cut(s) 245, 251, 311, 312
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 435
TaiI ACGT 7 cut(s) 127, 146, 159, 453, 461, 516, 543
TaqI TCGA 1 cut(s) 545
TaqII GACCGA 1 cut(s) 551
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 138, 417, 473
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 1 cut(s) 132
Tsp45I GTSAC 1 cut(s) 374
TspGWI ACGGA 2 cut(s) 512, 516
TspMI CCCGGG 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XmaI CCCGGG 1 cut(s) 312
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.