RLG00000005925

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004993
Physical Location & Seq
Reverse (-)
2847458 .. 2848419
962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005925

Sequence Viewer

Length: 612 bp
ATGAAAAACCCTCAATCCGTCCTCCTACTCCTCCCTCTCATCTTCCTCTTCTTCCTCCATCCAACCTTAGCCGTCGAATCCCCCTCGTCCTCTCCCACACCCACCACCAACCCTGCAGATTTCATCCGCACAAGTTGCGCCACCACCCTATACCCCGAGCTCTGCGACTCCTCCCTCTCACCCTTCACCTACACCGTCCAGTCAAACCCTACTAGGCTTGCGATGGCAGCCGTTGCCGTGAGCCTAACCAATGCAAGACACACGGCGACTTCCCTCGCAAGCCTCTCCAAACAAGCCGACCCTAAAGTCGCAGGGGCCCTCCAGGACTGTTCCAGCAACTTCGGAAAAGCCATCGACGAGATTACAGATTCACAAGAGCAGATGAAGACGTTGGGGTCGGGTACCGGGTCGGTTAGGTTTCAGCTGAGCAACGTGCAGACGTGGATGAGTGCGGCGCTTACGGACGCGGAGACGTGTACGGACGGATTTGAGGACGTGGAGGACGGGCCGATCAAGACGGAGGTGGTGAACCAGGCTGAGATGGTGAAGAAGATGACAAGTATTGCGCTTGCGATCGTTAATGGTCTTGCTAGTAACGGCGCACTGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

204

Amino Acids

21.46

Weight (kDa)

4.7

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 192 2.5e-39 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 305, 394
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 401
AccII CGCG 1 cut(s) 467
AciI CCGC 3 cut(s) 127, 452, 467
AfaI GTAC 2 cut(s) 403, 478
AflIII ACRYGT 1 cut(s) 473
AjiI CACGTC 3 cut(s) 441, 474, 496
AjnI CCWGG 2 cut(s) 321, 531
AluBI AGCT 2 cut(s) 160, 424
AluI AGCT 2 cut(s) 160, 424
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 2 cut(s) 315, 506
ApaI GGGCCC 1 cut(s) 319
ApeKI GCWGC 1 cut(s) 227
Asp718I GGTACC 1 cut(s) 401
AspLEI GCGC 4 cut(s) 140, 457, 568, 602
AspS9I GGNCC 3 cut(s) 315, 316, 506
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 4 cut(s) 171, 178, 538, 556
AvaI CYCGRG 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 319
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 2 cut(s) 162, 319
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 239
BccI CCATC 4 cut(s) 66, 217, 359, 535
BceAI ACGGC 4 cut(s) 56, 215, 221, 279
BciT130I CCWGG 2 cut(s) 323, 533
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 2 cut(s) 213, 591
BfmI CTRYAG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 458
BisI GCNGC 2 cut(s) 228, 453
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 2 cut(s) 229, 454
Bme1390I CCNGG 3 cut(s) 323, 406, 533
BmeT110I CYCGRG 1 cut(s) 155
BmgBI CACGTC 3 cut(s) 441, 474, 496
BmgT120I GGNCC 3 cut(s) 315, 316, 506
BmiI GGNNCC 3 cut(s) 316, 317, 403
BmrFI CCNGG 3 cut(s) 323, 406, 533
BpiI GAAGAC 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 406
Bse1I ACTGG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 323, 533
BseGI GGATG 3 cut(s) 58, 123, 450
BseMII CTCAG 2 cut(s) 416, 528
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 20, 160
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 467
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 2 cut(s) 317, 508
BshNI GGYRCC 1 cut(s) 401
BsiEI CGRYCG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 1 cut(s) 405
BsmAI GTCTC 1 cut(s) 464
BsmBI CGTCTC 1 cut(s) 464
BsnI GGCC 2 cut(s) 317, 508
BsoBI CYCGRG 1 cut(s) 155
Bsp120I GGGCCC 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 162, 319
Bsp143I GATC 2 cut(s) 510, 573
Bsp1720I GCTNAGC 1 cut(s) 425
BspACI CCGC 3 cut(s) 127, 452, 467
BspANI GGCC 2 cut(s) 317, 508
BspCNI CTCAG 2 cut(s) 417, 529
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 3 cut(s) 316, 317, 403
BspMAI CTGCAG 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 401
BsrI ACTGG 1 cut(s) 199
BssMI GATC 2 cut(s) 510, 573
Bst2UI CCWGG 2 cut(s) 323, 533
Bst4CI ACNGT 2 cut(s) 196, 329
Bst6I CTCTTC 1 cut(s) 53
BstAPI GCANNNNNTGC 2 cut(s) 135, 233
BstC8I GCNNGC 3 cut(s) 219, 280, 570
BstDEI CTNAG 3 cut(s) 67, 425, 537
BstF5I GGATG 3 cut(s) 58, 123, 450
BstFNI CGCG 1 cut(s) 467
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 4 cut(s) 140, 457, 568, 602
BstKTI GATC 2 cut(s) 513, 576
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 2 cut(s) 510, 573
BstMCI CGRYCG 1 cut(s) 576
BstMWI GCNNNNNNNGC 3 cut(s) 135, 227, 233
BstNI CCWGG 2 cut(s) 323, 533
BstSCI CCNGG 3 cut(s) 321, 404, 531
BstSFI CTRYAG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 319
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 1 cut(s) 239
BstV2I GAAGAC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 317, 508
BtgZI GCGATG 1 cut(s) 236
BtrI CACGTC 3 cut(s) 441, 474, 496
BtsCI GGATG 3 cut(s) 58, 123, 450
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 1 cut(s) 602
Cac8I GCNNGC 3 cut(s) 219, 280, 570
CfoI GCGC 4 cut(s) 140, 457, 568, 602
Cfr13I GGNCC 3 cut(s) 315, 316, 506
CseI GACGC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 402, 477
CviQI GTAC 2 cut(s) 402, 477
DdeI CTNAG 3 cut(s) 67, 425, 537
DpnI GATC 2 cut(s) 512, 575
DpnII GATC 2 cut(s) 510, 573
DrdI GACNNNNNNGTC 2 cut(s) 305, 394
DseDI GACNNNNNNGTC 2 cut(s) 305, 394
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 2 cut(s) 162, 319
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 2 cut(s) 315, 316
EcoRII CCWGG 2 cut(s) 321, 531
EcoT38I GRGCYC 2 cut(s) 162, 319
Esp3I CGTCTC 1 cut(s) 464
FaiI YATR 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 228, 453
FokI GGATG 3 cut(s) 45, 110, 457
FriOI GRGCYC 2 cut(s) 162, 319
Fsp4HI GCNGC 2 cut(s) 228, 453
FspBI CTAG 2 cut(s) 213, 591
GlaI GCGC 4 cut(s) 139, 456, 567, 601
GluI GCNGC 2 cut(s) 228, 453
GsuI CTGGAG 1 cut(s) 305
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 2 cut(s) 317, 508
HapII CCGG 1 cut(s) 405
HgaI GACGC 1 cut(s) 473
HhaI GCGC 4 cut(s) 140, 457, 568, 602
Hin6I GCGC 4 cut(s) 138, 455, 566, 600
HinP1I GCGC 4 cut(s) 138, 455, 566, 600
HinfI GANTC 3 cut(s) 77, 167, 368
HpaII CCGG 1 cut(s) 405
HphI GGTGA 4 cut(s) 171, 178, 538, 556
Hpy166II GTNNAC 2 cut(s) 477, 529
Hpy188I TCNGA 1 cut(s) 344
Hpy188III TCNNGA 1 cut(s) 514
Hpy8I GTNNAC 2 cut(s) 477, 529
Hpy99I CGWCG 2 cut(s) 77, 359
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 2 cut(s) 196, 329
HpyCH4IV ACGT 5 cut(s) 389, 432, 440, 473, 495
HpyCH4V TGCA 3 cut(s) 116, 254, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 135, 227, 233
HpyF3I CTNAG 3 cut(s) 67, 425, 537
HpySE526I ACGT 5 cut(s) 389, 432, 440, 473, 495
HspAI GCGC 4 cut(s) 138, 455, 566, 600
KpnI GGTACC 1 cut(s) 405
Kzo9I GATC 2 cut(s) 510, 573
LpnPI CCDG 9 cut(s) 126, 212, 297, 308, 335, 346, 418, 518, 545
Lsp1109I GCAGC 1 cut(s) 239
MaeI CTAG 2 cut(s) 213, 591
MaeII ACGT 5 cut(s) 389, 432, 440, 473, 495
MaeIII GTNAC 1 cut(s) 593
MalI GATC 2 cut(s) 512, 575
MboI GATC 2 cut(s) 510, 573
MboII GAAGA 6 cut(s) 34, 40, 43, 397, 559, 562
MhlI GDGCHC 2 cut(s) 162, 319
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 86
MseI TTAA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 1 cut(s) 405
MspR9I CCNGG 3 cut(s) 323, 406, 533
MvaI CCWGG 2 cut(s) 323, 533
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 3 cut(s) 135, 227, 233
NciI CCSGG 1 cut(s) 406
NdeII GATC 2 cut(s) 510, 573
NlaIV GGNNCC 3 cut(s) 316, 317, 403
PfeI GAWTC 2 cut(s) 77, 368
PfoI TCCNGGA 1 cut(s) 321
PkrI GCNGC 2 cut(s) 229, 454
Ple19I CGATCG 1 cut(s) 576
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 321, 531
PspGI CCWGG 2 cut(s) 321, 531
PspN4I GGNNCC 3 cut(s) 316, 317, 403
PspOMI GGGCCC 1 cut(s) 315
PspPI GGNCC 3 cut(s) 315, 316, 506
PstI CTGCAG 1 cut(s) 118
PvuI CGATCG 1 cut(s) 576
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 2 cut(s) 403, 478
RsaNI GTAC 2 cut(s) 402, 477
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 2 cut(s) 228, 453
Sau3AI GATC 2 cut(s) 510, 573
Sau96I GGNCC 3 cut(s) 315, 316, 506
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 3 cut(s) 323, 406, 533
SduI GDGCHC 2 cut(s) 162, 319
SfcI CTRYAG 1 cut(s) 114
SsiI CCGC 3 cut(s) 127, 452, 467
SspMI CTAG 2 cut(s) 213, 591
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 321, 404, 531
TaaI ACNGT 2 cut(s) 196, 329
TaiI ACGT 5 cut(s) 392, 435, 443, 476, 498
TaqI TCGA 2 cut(s) 75, 354
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 2 cut(s) 77, 368
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TscAI CASTG 1 cut(s) 609
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 3 cut(s) 17, 112, 398
TspGWI ACGGA 5 cut(s) 7, 476, 494, 498, 533
TspRI CASTG 1 cut(s) 609
XspI CTAG 2 cut(s) 213, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.