Rh4AG402100

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004993
Physical Location & Seq
Forward (+)
70467799 .. 70474005
6207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG402100.1

Sequence Viewer

Length: 345 bp
ATGGCAGCCATCGCTGTGAGCCTAACCAATGCCAGACGCACGGCGACTTCCCTCGCAAGCCTCTCCAAACAAGCCGACCCTAAAGTCGCCGGGGCCCTCCAGGACTGCTCCAGCAACTTCGGAAAAGCCATCGACGAGATTACAGATTCCCAAGAGCAGATGAAGACGTTGGGGTCGGGTACCGGGTCGGTTAGGTTTCAGCTGAGCAACGTGCAGACTTGGATGAGTGCGGCGCTTACGGACGCGGAGACGTGTACGGATGGATTTGAGGACGTGGAGGACGGGCCGATCAAGACGGAGGTGGTGAACCAGGCTGAGATGGTGAAGAAGATGACAATTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

114

Amino Acids

12.05

Weight (kDa)

4.56

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 2 - 112 2.5e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 83, 172
Acc65I GGTACC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 1 cut(s) 245
AciI CCGC 2 cut(s) 230, 245
AfaI GTAC 2 cut(s) 181, 256
AflIII ACRYGT 1 cut(s) 251
AjiI CACGTC 2 cut(s) 252, 274
AjnI CCWGG 2 cut(s) 99, 309
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 242
AoxI GGCC 2 cut(s) 93, 284
ApaI GGGCCC 1 cut(s) 97
ApeKI GCWGC 1 cut(s) 5
Asp718I GGTACC 1 cut(s) 179
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 3 cut(s) 93, 94, 284
AsuC2I CCSGG 2 cut(s) 91, 184
AsuHPI GGTGA 2 cut(s) 316, 334
BaeGI GKGCMC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 97
BbsI GAAGAC 1 cut(s) 170
BbvI GCAGC 1 cut(s) 17
BccI CCATC 4 cut(s) 17, 137, 254, 313
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 2 cut(s) 101, 311
BcnI CCSGG 2 cut(s) 91, 184
BcoDI GTCTC 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 236
BisI GCNGC 2 cut(s) 6, 231
BlpI GCTNAGC 1 cut(s) 203
BlsI GCNGC 2 cut(s) 7, 232
Bme1390I CCNGG 4 cut(s) 91, 101, 184, 311
BmgBI CACGTC 2 cut(s) 252, 274
BmgT120I GGNCC 3 cut(s) 93, 94, 284
BmiI GGNNCC 3 cut(s) 94, 95, 181
BmrFI CCNGG 4 cut(s) 91, 101, 184, 311
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 83, 94
Bpu1102I GCTNAGC 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 91, 184
BsaJI CCNNGG 1 cut(s) 90
BseBI CCWGG 2 cut(s) 101, 311
BseDI CCNNGG 1 cut(s) 90
BseGI GGATG 2 cut(s) 228, 265
BseMII CTCAG 2 cut(s) 194, 306
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 1 cut(s) 17
BsgI GTGCAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 245
BshFI GGCC 2 cut(s) 95, 286
BshNI GGYRCC 1 cut(s) 179
BsiSI CCGG 2 cut(s) 90, 183
BsmAI GTCTC 1 cut(s) 242
BsmBI CGTCTC 1 cut(s) 242
BsnI GGCC 2 cut(s) 95, 286
Bsp120I GGGCCC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 1 cut(s) 288
Bsp1720I GCTNAGC 1 cut(s) 203
BspACI CCGC 2 cut(s) 230, 245
BspANI GGCC 2 cut(s) 95, 286
BspCNI CTCAG 2 cut(s) 195, 307
BspFNI CGCG 1 cut(s) 245
BspLI GGNNCC 3 cut(s) 94, 95, 181
BspT107I GGYRCC 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 90
BssMI GATC 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 101, 311
BstC8I GCNNGC 1 cut(s) 58
BstDEI CTNAG 2 cut(s) 203, 315
BstF5I GGATG 2 cut(s) 228, 265
BstFNI CGCG 1 cut(s) 245
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 1 cut(s) 242
BstMBI GATC 1 cut(s) 288
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 101, 311
BstSCI CCNGG 4 cut(s) 89, 99, 182, 309
BstSLI GKGCMC 1 cut(s) 97
BstUI CGCG 1 cut(s) 245
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 95, 286
BtrI CACGTC 2 cut(s) 252, 274
BtsCI GGATG 2 cut(s) 228, 265
Cac8I GCNNGC 1 cut(s) 58
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 3 cut(s) 93, 94, 284
CseI GACGC 2 cut(s) 45, 251
Csp6I GTAC 2 cut(s) 180, 255
CviJI RGCY 9 cut(s) 8, 21, 60, 74, 95, 128, 202, 286, 314
CviKI_1 RGCY 9 cut(s) 8, 21, 60, 74, 95, 128, 202, 286, 314
CviQI GTAC 2 cut(s) 180, 255
DdeI CTNAG 2 cut(s) 203, 315
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
DrdI GACNNNNNNGTC 2 cut(s) 83, 172
DseDI GACNNNNNNGTC 2 cut(s) 83, 172
Eco24I GRGCYC 1 cut(s) 97
EcoO109I RGGNCCY 2 cut(s) 93, 94
EcoRII CCWGG 2 cut(s) 99, 309
EcoT38I GRGCYC 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 242
Fnu4HI GCNGC 2 cut(s) 6, 231
FokI GGATG 2 cut(s) 235, 272
FriOI GRGCYC 1 cut(s) 97
Fsp4HI GCNGC 2 cut(s) 6, 231
GlaI GCGC 1 cut(s) 234
GluI GCNGC 2 cut(s) 6, 231
GsuI CTGGAG 2 cut(s) 83, 94
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 2 cut(s) 95, 286
HapII CCGG 2 cut(s) 90, 183
HgaI GACGC 2 cut(s) 45, 251
HhaI GCGC 1 cut(s) 235
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HinfI GANTC 1 cut(s) 146
HpaII CCGG 2 cut(s) 90, 183
HphI GGTGA 2 cut(s) 316, 334
Hpy166II GTNNAC 2 cut(s) 255, 307
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 1 cut(s) 292
Hpy8I GTNNAC 2 cut(s) 255, 307
Hpy99I CGWCG 1 cut(s) 137
HpyCH4IV ACGT 4 cut(s) 167, 210, 251, 273
HpyCH4V TGCA 1 cut(s) 214
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 2 cut(s) 203, 315
HpySE526I ACGT 4 cut(s) 167, 210, 251, 273
HspAI GCGC 1 cut(s) 233
KpnI GGTACC 1 cut(s) 183
Kzo9I GATC 1 cut(s) 288
LmnI GCTCC 1 cut(s) 113
LpnPI CCDG 8 cut(s) 46, 86, 103, 113, 124, 196, 296, 323
Lsp1109I GCAGC 1 cut(s) 17
MaeII ACGT 4 cut(s) 167, 210, 251, 273
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 3 cut(s) 175, 337, 340
MhlI GDGCHC 1 cut(s) 97
MluCI AATT 1 cut(s) 336
MnlI CCTC 6 cut(s) 62, 71, 107, 262, 271, 292
MslI CAYNNNNRTG 1 cut(s) 14
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 2 cut(s) 90, 183
MspR9I CCNGG 4 cut(s) 91, 101, 184, 311
MvaI CCWGG 2 cut(s) 101, 311
MvnI CGCG 1 cut(s) 245
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 2 cut(s) 91, 184
NdeII GATC 1 cut(s) 288
NlaIV GGNNCC 3 cut(s) 94, 95, 181
PfeI GAWTC 1 cut(s) 146
PfoI TCCNGGA 1 cut(s) 99
PkrI GCNGC 2 cut(s) 7, 232
Psp6I CCWGG 2 cut(s) 99, 309
PspGI CCWGG 2 cut(s) 99, 309
PspN4I GGNNCC 3 cut(s) 94, 95, 181
PspOMI GGGCCC 1 cut(s) 93
PspPI GGNCC 3 cut(s) 93, 94, 284
PvuII CAGCTG 1 cut(s) 202
RsaI GTAC 2 cut(s) 181, 256
RsaNI GTAC 2 cut(s) 180, 255
RseI CAYNNNNRTG 1 cut(s) 14
SatI GCNGC 2 cut(s) 6, 231
Sau3AI GATC 1 cut(s) 288
Sau96I GGNCC 3 cut(s) 93, 94, 284
ScrFI CCNGG 4 cut(s) 91, 101, 184, 311
SduI GDGCHC 1 cut(s) 97
SetI ASST 7 cut(s) 170, 197, 204, 213, 254, 276, 303
SmiMI CAYNNNNRTG 1 cut(s) 14
Sse9I AATT 1 cut(s) 336
SsiI CCGC 2 cut(s) 230, 245
StyD4I CCNGG 4 cut(s) 89, 99, 182, 309
TaiI ACGT 4 cut(s) 170, 213, 254, 276
TaqI TCGA 1 cut(s) 132
TasI AATT 1 cut(s) 336
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 146
TseI GCWGC 1 cut(s) 5
TspDTI ATGAA 1 cut(s) 176
TspGWI ACGGA 3 cut(s) 254, 272, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.