AT1G18210

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007579
Physical Location & Seq
Reverse (-)
6266609 .. 6268939
2331 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G18210.1

Sequence Viewer

Length: 513 bp
ATGGCAAGCGCGAATCCAGAAACCGCCAAACCCACGCCTGCAACGGTGGACATGGCTAATCCAGAGGAGCTTAAGAAAGTTTTCGACCAGTTTGACTCCAACGGCGACGGCAAGATCTCAGTCCTCGAACTCGGCGGTGTGTTTAAGGCTATGGGGACTTCCTACACAGAGACGGAACTCAACCGCGTGCTCGAAGAAGTCGATACAGATCGCGACGGTTACATCAACCTCGACGAATTCTCAACTCTCTGTCGCTCGTCTTCCTCCGCCGCAGAGATCCGTGACGCTTTCGATCTCTACGATCAGGACAAAAACGGACTGATCTCCGCCTCTGAGCTTCACCAGGTGCTTAACCGTCTCGGGATGTCTTGCTCTGTTGAAGACTGTACTCGTATGATCGGACCTGTGGATGCTGACGGCGATGGGAATGTTAATTTCGAAGAGTTTCAGAAGATGATGACGTCGTCTTCTCTCCTCAACAGCAATGGATCCGCCGCTCCTCCTTCGACCTAG

Protein Analysis

170

Amino Acids

18.35

Weight (kDa)

4.19

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 22 - 84 1.7e-15 EF-hand domain pair
EF-hand_1 PF00036 23 - 51 2.6e-06 EF hand domain
EF-hand_6 PF13405 23 - 52 2.2e-07 EF-hand domain
EF-hand_5 PF13202 24 - 45 5e-07 EF hand
EF-hand_1 PF00036 59 - 86 1.6e-06 EF hand domain
EF-hand_7 PF13499 92 - 154 2.4e-14 EF-hand domain pair
EF-hand_1 PF00036 92 - 119 1.2e-06 EF hand domain
EF-hand_6 PF13405 92 - 121 6.3e-08 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 497
AccII CGCG 3 cut(s) 11, 186, 213
AciI CCGC 8 cut(s) 24, 135, 184, 267, 270, 327, 492, 495
AclWI GGATC 3 cut(s) 271, 483, 496
AcsI RAATTY 1 cut(s) 236
AcyI GRCGYC 1 cut(s) 461
AdeI CACNNNGTG 1 cut(s) 346
AfaI GTAC 1 cut(s) 388
AflII CTTAAG 1 cut(s) 71
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 1 cut(s) 342
AluBI AGCT 2 cut(s) 70, 337
AluI AGCT 2 cut(s) 70, 337
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 164, 362
AlwI GGATC 3 cut(s) 271, 483, 496
Ama87I CYCGRG 1 cut(s) 359
ApoI RAATTY 1 cut(s) 236
Asp700I GAANNNNTTC 2 cut(s) 80, 444
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 401
AsuHPI GGTGA 1 cut(s) 332
AsuII TTCGAA 1 cut(s) 438
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 401
BamHI GGATCC 1 cut(s) 488
BbsI GAAGAC 3 cut(s) 252, 387, 459
Bbv12I GWGCWC 1 cut(s) 192
BccI CCATC 1 cut(s) 416
BceAI ACGGC 3 cut(s) 118, 124, 433
BciT130I CCWGG 1 cut(s) 344
BcoDI GTCTC 2 cut(s) 164, 362
BfaI CTAG 1 cut(s) 511
BfrI CTTAAG 1 cut(s) 71
BglII AGATCT 1 cut(s) 114
BisI GCNGC 2 cut(s) 270, 495
BlsI GCNGC 2 cut(s) 271, 496
Bme1390I CCNGG 1 cut(s) 344
Bme18I GGWCC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 1 cut(s) 344
BmsI GCATC 1 cut(s) 400
BpiI GAAGAC 3 cut(s) 252, 387, 459
Bpu14I TTCGAA 1 cut(s) 438
BsaBI GATNNNNATC 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 344
BseGI GGATG 2 cut(s) 369, 415
BseJI GATNNNNATC 1 cut(s) 207
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 132, 324
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 3 cut(s) 80, 464, 489
Bsh1236I CGCG 3 cut(s) 11, 186, 213
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 164, 362
BsmBI CGTCTC 2 cut(s) 164, 362
BsmFI GGGAC 1 cut(s) 169
BsoBI CYCGRG 1 cut(s) 359
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
Bsp68I TCGCGA 1 cut(s) 213
BspACI CCGC 8 cut(s) 24, 135, 184, 267, 270, 327, 492, 495
BspCNI CTCAG 2 cut(s) 131, 325
BspFNI CGCG 3 cut(s) 11, 186, 213
BspLI GGNNCC 1 cut(s) 490
BspPI GGATC 3 cut(s) 271, 483, 496
BspT104I TTCGAA 1 cut(s) 438
BspTI CTTAAG 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 1 cut(s) 88
BssMI GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
BssNI GRCGYC 1 cut(s) 461
Bst2UI CCWGG 1 cut(s) 344
Bst4CI ACNGT 4 cut(s) 46, 218, 356, 386
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 1 cut(s) 461
BstAFI CTTAAG 1 cut(s) 71
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 3 cut(s) 7, 39, 188
BstDEI CTNAG 2 cut(s) 118, 333
BstF5I GGATG 2 cut(s) 369, 415
BstFNI CGCG 3 cut(s) 11, 186, 213
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 8 cut(s) 117, 211, 279, 295, 304, 324, 399, 491
BstMAI GTCTC 2 cut(s) 164, 362
BstMBI GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
BstNI CCWGG 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 342
BstUI CGCG 3 cut(s) 11, 186, 213
BstV2I GAAGAC 3 cut(s) 252, 387, 459
BstX2I RGATCY 3 cut(s) 114, 276, 488
BstYI RGATCY 3 cut(s) 114, 276, 488
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 2 cut(s) 369, 415
BtuMI TCGCGA 1 cut(s) 213
Cac8I GCNNGC 3 cut(s) 7, 39, 188
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 401
CseI GACGC 1 cut(s) 293
CsiI ACCWGGT 1 cut(s) 342
Csp6I GTAC 1 cut(s) 387
CviAII CATG 1 cut(s) 52
CviJI RGCY 4 cut(s) 56, 70, 149, 337
CviKI_1 RGCY 4 cut(s) 56, 70, 149, 337
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 2 cut(s) 118, 333
DpnI GATC 8 cut(s) 116, 210, 278, 294, 303, 323, 398, 490
DpnII GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
DraIII CACNNNGTG 1 cut(s) 346
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 3 cut(s) 256, 316, 481
Eco47I GGWCC 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 359
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 342
Esp3I CGTCTC 2 cut(s) 164, 362
FaeI CATG 1 cut(s) 55
FaiI YATR 3 cut(s) 53, 152, 395
FaqI GGGAC 1 cut(s) 169
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 2 cut(s) 270, 495
FokI GGATG 2 cut(s) 376, 422
Fsp4HI GCNGC 2 cut(s) 270, 495
FspBI CTAG 1 cut(s) 511
GlaI GCGC 1 cut(s) 10
GluI GCNGC 2 cut(s) 270, 495
HgaI GACGC 1 cut(s) 293
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 461
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 2 cut(s) 13, 95
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 334, 401, 450
Hpy188III TCNNGA 5 cut(s) 17, 62, 212, 305, 361
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 4 cut(s) 110, 218, 236, 466
HpyAV CCTTC 1 cut(s) 513
HpyCH4III ACNGT 4 cut(s) 46, 218, 356, 386
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 1 cut(s) 41
HpyF3I CTNAG 2 cut(s) 118, 333
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 1 cut(s) 461
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
LmnI GCTCC 2 cut(s) 67, 502
LpnPI CCDG 8 cut(s) 30, 51, 75, 101, 290, 329, 356, 417
LweI GCATC 1 cut(s) 400
MabI ACCWGGT 1 cut(s) 342
MaeI CTAG 1 cut(s) 511
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 2 cut(s) 218, 281
MalI GATC 8 cut(s) 116, 210, 278, 294, 303, 323, 398, 490
MbiI CCGCTC 1 cut(s) 497
MboI GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
MboII GAAGA 6 cut(s) 206, 252, 392, 452, 459, 463
MflI RGATCY 3 cut(s) 114, 276, 488
MhlI GDGCHC 1 cut(s) 192
MluCI AATT 2 cut(s) 236, 433
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 7 cut(s) 58, 134, 239, 274, 340, 485, 510
MroXI GAANNNNTTC 2 cut(s) 80, 444
MseI TTAA 4 cut(s) 72, 144, 351, 432
MspCI CTTAAG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 344
MvaI CCWGG 1 cut(s) 344
MvnI CGCG 3 cut(s) 11, 186, 213
NdeII GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 490
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 281
NruI TCGCGA 1 cut(s) 213
NspV TTCGAA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 297
PdmI GAANNNNTTC 2 cut(s) 80, 444
PfeI GAWTC 1 cut(s) 13
PflFI GACNNNGTC 1 cut(s) 463
PkrI GCNGC 2 cut(s) 271, 496
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 342
PspGI CCWGG 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 1 cut(s) 401
PsuI RGATCY 3 cut(s) 114, 276, 488
PsyI GACNNNGTC 1 cut(s) 463
RruI TCGCGA 1 cut(s) 213
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 4 cut(s) 72, 144, 351, 432
SatI GCNGC 2 cut(s) 270, 495
Sau3AI GATC 8 cut(s) 114, 208, 276, 292, 301, 321, 396, 488
Sau96I GGNCC 1 cut(s) 401
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 344
SduI GDGCHC 1 cut(s) 192
SetI ASST 7 cut(s) 72, 231, 339, 348, 406, 464, 512
SexAI ACCWGGT 1 cut(s) 342
SfaNI GCATC 1 cut(s) 400
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 1 cut(s) 401
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 2 cut(s) 236, 433
SsiI CCGC 8 cut(s) 24, 135, 184, 267, 270, 327, 492, 495
SspMI CTAG 1 cut(s) 511
StyD4I CCNGG 1 cut(s) 342
TaaI ACNGT 4 cut(s) 46, 218, 356, 386
TaiI ACGT 1 cut(s) 464
TaqI TCGA 8 cut(s) 84, 126, 192, 201, 231, 291, 438, 506
TasI AATT 2 cut(s) 236, 433
TatI WGTACW 1 cut(s) 386
TauI GCSGC 2 cut(s) 272, 497
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 4 cut(s) 72, 144, 351, 432
Tru9I TTAA 4 cut(s) 72, 144, 351, 432
TseFI GTSAC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 281
TspGWI ACGGA 3 cut(s) 188, 269, 330
Tth111I GACNNNGTC 1 cut(s) 463
Vha464I CTTAAG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 401
XapI RAATTY 1 cut(s) 236
XmnI GAANNNNTTC 2 cut(s) 80, 444
XspI CTAG 1 cut(s) 511
ZraI GACGTC 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.