AT1G73630

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007579
Physical Location & Seq
Forward (+)
27684673 .. 27685614
942 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G73630.1

Sequence Viewer

Length: 492 bp
ATGGCAAACACAAATCTTGAATCCACCAACAAATCCACAACACCATCAACGGATATGGAGCTAAAGAAAGTCTTCGACAAGTTCGATGCCAACGGCGACGGGAAAATCTCAGTTTCAGAACTCGGAAACGTTTTCAAATCCATGGGAACGTCGTACACTGAGGAGGAGCTAAACCGTGTATTAGACGAAATCGACATCGACTGCGACGGTTTCATCAATCAAGAAGAGTTTGCTACCATTTGTCGATCATCTTCCTCCGCTGTAGAGATCCGTGAAGCGTTCGATCTCTACGATCAGAATAAAAACGGTTTGATTTCGTCCTCTGAGATCCATAAAGTTCTGAATCGTCTTGGTATGACTTGCTCTGTTGAAGATTGTGTGAGAATGATTGGTCATGTTGATACTGATGGCGATGGAAATGTTAATTTCGAGGAGTTTCAGAAGATGATGTCGTCGCCTGAGCTCGTTAAGGGAACCGTTGCGAACTCTTAG

Protein Analysis

163

Amino Acids

18.0

Weight (kDa)

4.39

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 19 - 81 8.4e-16 EF-hand domain pair
EF-hand_1 PF00036 20 - 48 3.6e-08 EF hand domain
EF-hand_6 PF13405 20 - 49 1.4e-08 EF-hand domain
EF-hand_5 PF13202 21 - 44 1.6e-08 EF hand
EF-hand_1 PF00036 89 - 116 6.5e-06 EF hand domain
EF-hand_7 PF13499 89 - 151 1.1e-13 EF-hand domain pair
EF-hand_6 PF13405 89 - 118 6.4e-07 EF-hand domain
EF-hand_1 PF00036 127 - 151 5.6e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 258
AclI AACGTT 1 cut(s) 129
AclWI GGATC 2 cut(s) 262, 322
AfaI GTAC 1 cut(s) 155
AgsI TTSAA 3 cut(s) 20, 136, 371
AluBI AGCT 3 cut(s) 61, 169, 463
AluI AGCT 3 cut(s) 61, 169, 463
Alw21I GWGCWC 1 cut(s) 465
AlwI GGATC 2 cut(s) 262, 322
Asp700I GAANNNNTTC 1 cut(s) 71
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 465
BccI CCATC 3 cut(s) 52, 401, 407
BceAI ACGGC 1 cut(s) 109
BfmI CTRYAG 1 cut(s) 261
BmiI GGNNCC 1 cut(s) 475
BmsI GCATC 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 141
BseDI CCNNGG 1 cut(s) 141
BseMII CTCAG 4 cut(s) 123, 150, 315, 450
BseRI GAGGAG 3 cut(s) 176, 179, 446
BsiHKAI GWGCWC 1 cut(s) 465
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 5 cut(s) 245, 267, 283, 292, 327
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 1 cut(s) 258
BspCNI CTCAG 4 cut(s) 122, 151, 316, 451
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 2 cut(s) 262, 322
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 5 cut(s) 245, 267, 283, 292, 327
BssT1I CCWWGG 1 cut(s) 141
Bst4CI ACNGT 4 cut(s) 176, 209, 308, 478
Bst6I CTCTTC 1 cut(s) 219
BstDEI CTNAG 5 cut(s) 109, 159, 324, 459, 489
BstDSI CCRYGG 1 cut(s) 141
BstKTI GATC 5 cut(s) 248, 270, 286, 295, 330
BstMBI GATC 5 cut(s) 245, 267, 283, 292, 327
BstSFI CTRYAG 1 cut(s) 261
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 2 cut(s) 267, 327
BstYI RGATCY 2 cut(s) 267, 327
BtgI CCRYGG 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 156
Csp6I GTAC 1 cut(s) 154
CviAII CATG 2 cut(s) 142, 395
CviJI RGCY 3 cut(s) 61, 169, 463
CviKI_1 RGCY 3 cut(s) 61, 169, 463
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 5 cut(s) 109, 159, 324, 459, 489
DpnI GATC 5 cut(s) 247, 269, 285, 294, 329
DpnII GATC 5 cut(s) 245, 267, 283, 292, 327
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 2 cut(s) 145, 398
FaiI YATR 5 cut(s) 56, 143, 333, 356, 396
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 2 cut(s) 141, 394
FriOI GRGCYC 1 cut(s) 465
Hin1II CATG 2 cut(s) 145, 398
HinfI GANTC 2 cut(s) 20, 343
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 6 cut(s) 118, 125, 297, 325, 342, 441
Hpy188III TCNNGA 2 cut(s) 17, 221
Hpy8I GTNNAC 1 cut(s) 156
Hpy99I CGWCG 4 cut(s) 101, 154, 209, 457
HpyCH4III ACNGT 4 cut(s) 176, 209, 308, 478
HpyCH4IV ACGT 2 cut(s) 129, 149
HpyF3I CTNAG 5 cut(s) 109, 159, 324, 459, 489
HpySE526I ACGT 2 cut(s) 129, 149
Hsp92II CATG 2 cut(s) 145, 398
Kzo9I GATC 5 cut(s) 245, 267, 283, 292, 327
LmnI GCTCC 2 cut(s) 58, 166
LpnPI CCDG 1 cut(s) 471
LweI GCATC 1 cut(s) 76
MaeII ACGT 2 cut(s) 129, 149
MalI GATC 5 cut(s) 247, 269, 285, 294, 329
MboI GATC 5 cut(s) 245, 267, 283, 292, 327
MboII GAAGA 5 cut(s) 64, 236, 243, 383, 454
MflI RGATCY 2 cut(s) 267, 327
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 1 cut(s) 424
MnlI CCTC 5 cut(s) 154, 157, 265, 331, 424
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 2 cut(s) 423, 468
MspA1I CMGCKG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 141
NdeII GATC 5 cut(s) 245, 267, 283, 292, 327
NlaIII CATG 2 cut(s) 145, 398
NlaIV GGNNCC 1 cut(s) 475
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 288
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 20, 343
Psp124BI GAGCTC 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 475
PsuI RGATCY 2 cut(s) 267, 327
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
SacI GAGCTC 1 cut(s) 465
SaqAI TTAA 2 cut(s) 423, 468
Sau3AI GATC 5 cut(s) 245, 267, 283, 292, 327
SduI GDGCHC 1 cut(s) 465
SetI ASST 5 cut(s) 63, 132, 152, 171, 465
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 261
Sse9I AATT 1 cut(s) 424
SsiI CCGC 1 cut(s) 258
SstI GAGCTC 1 cut(s) 465
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 4 cut(s) 176, 209, 308, 478
TaiI ACGT 2 cut(s) 132, 152
TaqI TCGA 7 cut(s) 75, 84, 192, 198, 244, 282, 429
TasI AATT 1 cut(s) 424
TfiI GAWTC 2 cut(s) 20, 343
Tru1I TTAA 2 cut(s) 423, 468
Tru9I TTAA 2 cut(s) 423, 468
TscAI CASTG 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 202
TspGWI ACGGA 2 cut(s) 65, 260
TspRI CASTG 1 cut(s) 163
XmnI GAANNNNTTC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.