FvH4_5g17240

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007579
Physical Location & Seq
Reverse (-)
9849873 .. 9850385
513 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17240.t1

Sequence Viewer

Length: 513 bp
ATGGCTTCCAACGGAACCCCAACCCCTCCCCCCGCCGACGCCAAACCCATCGTCACCGACGAGGTCCGCCGCGTCTTCAAGAGCTTCGACACCAACGGCGACGGCAGGATCTCCGTCTCCGAGCTCGGCAACGTCCTCAAGGCCCTCGGCTCCAACGTCGGCGAAGACGAGCTCCAGCGCGTGATGGTCGAACTCGACACCGACCGCGACGGCTTCATCTGCCTCGACGAGTTCAACGCCTTCTGGGTGGCCGGCTCCCCTGACGGCTCCTCTGCCGAGCTCAAGGGAGCCTTCGATCTCTACGACCAGGACCGCAACGGCCTCATCTCCGCCGAAGAGCTCCACCTCGTCCTCAACCGCCTCCAGATGAAGTGCTCCGTCGAGGACTGCAACCGCATGATCCAGACCGTTGACGCCGACGGCGACGGCAATGTGAATTTCGAGGAGTTCAAGAAGATGATGAGCAACAACAGCGCCAGCAACGCCGGCACCGTCGCTTCTAACGGCGCGTGA

Protein Analysis

171

Amino Acids

18.33

Weight (kDa)

4.32

Isoelectric Point (pI)

22.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 21 - 49 2.4e-07 EF hand domain
EF-hand_7 PF13499 21 - 81 8.2e-15 EF-hand domain pair
EF-hand_6 PF13405 21 - 50 1.3e-08 EF-hand domain
EF-hand_5 PF13202 24 - 45 3.2e-07 EF hand
EF-hand_7 PF13499 93 - 155 2.5e-14 EF-hand domain pair
EF-hand_6 PF13405 93 - 120 7.7e-06 EF-hand domain
EF-hand_8 PF13833 106 - 155 3.9e-08 EF-hand domain pair
EF-hand_1 PF00036 130 - 155 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 488
AccII CGCG 4 cut(s) 72, 180, 207, 509
AciI CCGC 8 cut(s) 33, 67, 70, 205, 313, 330, 358, 394
AclWI GGATC 2 cut(s) 116, 394
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 436
AcyI GRCGYC 2 cut(s) 39, 414
AgsI TTSAA 3 cut(s) 79, 235, 451
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 5 cut(s) 84, 124, 172, 280, 340
AluI AGCT 5 cut(s) 84, 124, 172, 280, 340
Alw21I GWGCWC 5 cut(s) 126, 174, 282, 342, 377
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 116, 394
AoxI GGCC 3 cut(s) 141, 249, 319
ApoI RAATTY 1 cut(s) 436
ArsI GACNNNNNNTTYG 2 cut(s) 36, 68
AspLEI GCGC 3 cut(s) 180, 476, 509
AspS9I GGNCC 3 cut(s) 64, 142, 310
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 2 cut(s) 64, 310
BanI GGYRCC 1 cut(s) 488
BanII GRGCYC 4 cut(s) 126, 174, 282, 342
BbsI GAAGAC 2 cut(s) 67, 171
Bbv12I GWGCWC 5 cut(s) 126, 174, 282, 342, 377
BccI CCATC 2 cut(s) 56, 178
BceAI ACGGC 7 cut(s) 112, 118, 226, 280, 334, 436, 442
BciT130I CCWGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 121
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 1 cut(s) 70
BlsI GCNGC 1 cut(s) 71
Bme1390I CCNGG 1 cut(s) 308
Bme18I GGWCC 2 cut(s) 64, 310
BmgT120I GGNCC 3 cut(s) 64, 142, 310
BmiI GGNNCC 6 cut(s) 16, 151, 256, 268, 289, 490
BmrFI CCNGG 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 67, 171
BpmI CTGGAG 2 cut(s) 158, 347
BpuEI CTTGAG 2 cut(s) 122, 266
BsaHI GRCGYC 2 cut(s) 39, 414
BsaJI CCNNGG 1 cut(s) 145
Bse118I RCCGGY 2 cut(s) 251, 485
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 145
BseMI GCAATG 1 cut(s) 436
BseRI GAGGAG 2 cut(s) 259, 458
Bsh1236I CGCG 4 cut(s) 72, 180, 207, 509
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 143, 251, 321
BshNI GGYRCC 1 cut(s) 488
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 5 cut(s) 126, 174, 282, 342, 377
BsiSI CCGG 2 cut(s) 252, 486
BsmAI GTCTC 1 cut(s) 121
BsmBI CGTCTC 1 cut(s) 121
BsnI GGCC 3 cut(s) 143, 251, 321
Bsp1286I GDGCHC 5 cut(s) 126, 174, 282, 342, 377
Bsp143I GATC 3 cut(s) 108, 295, 399
BspACI CCGC 8 cut(s) 33, 67, 70, 205, 313, 330, 358, 394
BspANI GGCC 3 cut(s) 143, 251, 321
BspFNI CGCG 4 cut(s) 72, 180, 207, 509
BspLI GGNNCC 6 cut(s) 16, 151, 256, 268, 289, 490
BspPI GGATC 2 cut(s) 116, 394
BspQI GCTCTTC 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 2 cut(s) 251, 485
BssAI RCCGGY 2 cut(s) 251, 485
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 3 cut(s) 108, 295, 399
BssNI GRCGYC 2 cut(s) 39, 414
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 409, 493
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 2 cut(s) 39, 414
BstC8I GCNNGC 3 cut(s) 253, 478, 487
BstFNI CGCG 4 cut(s) 72, 180, 207, 509
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 3 cut(s) 180, 476, 509
BstKTI GATC 3 cut(s) 111, 298, 402
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 3 cut(s) 108, 295, 399
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 4 cut(s) 219, 471, 482, 486
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 306
BstUI CGCG 4 cut(s) 72, 180, 207, 509
BstV2I GAAGAC 2 cut(s) 67, 171
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 3 cut(s) 143, 251, 321
Cac8I GCNNGC 3 cut(s) 253, 478, 487
CfoI GCGC 3 cut(s) 180, 476, 509
Cfr10I RCCGGY 2 cut(s) 251, 485
Cfr13I GGNCC 3 cut(s) 64, 142, 310
CseI GACGC 3 cut(s) 47, 61, 422
CviAII CATG 1 cut(s) 397
DpnI GATC 3 cut(s) 110, 297, 401
DpnII GATC 3 cut(s) 108, 295, 399
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 2 cut(s) 56, 319
Ecl136II GAGCTC 4 cut(s) 124, 172, 280, 340
Eco24I GRGCYC 4 cut(s) 126, 174, 282, 342
Eco47I GGWCC 2 cut(s) 64, 310
Eco53kI GAGCTC 4 cut(s) 124, 172, 280, 340
EcoICRI GAGCTC 4 cut(s) 124, 172, 280, 340
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 306
EcoT38I GRGCYC 4 cut(s) 126, 174, 282, 342
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 1 cut(s) 400
FaiI YATR 1 cut(s) 398
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 1 cut(s) 396
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 70
FriOI GRGCYC 4 cut(s) 126, 174, 282, 342
Fsp4HI GCNGC 1 cut(s) 70
GlaI GCGC 3 cut(s) 179, 475, 508
GluI GCNGC 1 cut(s) 70
GsuI CTGGAG 2 cut(s) 158, 347
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 3 cut(s) 143, 251, 321
HapII CCGG 2 cut(s) 252, 486
HgaI GACGC 3 cut(s) 47, 61, 422
HhaI GCGC 3 cut(s) 180, 476, 509
Hin1I GRCGYC 2 cut(s) 39, 414
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 3 cut(s) 178, 474, 507
HinP1I GCGC 3 cut(s) 178, 474, 507
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HpaII CCGG 2 cut(s) 252, 486
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 4 cut(s) 79, 364, 403, 451
Hpy8I GTNNAC 1 cut(s) 412
HpyAV CCTTC 2 cut(s) 250, 301
HpyCH4III ACNGT 2 cut(s) 409, 493
HpyCH4IV ACGT 2 cut(s) 132, 156
HpyCH4V TGCA 1 cut(s) 390
HpyF10VI GCNNNNNNNGC 4 cut(s) 219, 471, 482, 486
HpySE526I ACGT 2 cut(s) 132, 156
Hsp92I GRCGYC 2 cut(s) 39, 414
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 3 cut(s) 178, 474, 507
KroI GCCGGC 2 cut(s) 251, 485
KroNI GCCGGC 2 cut(s) 253, 487
Kzo9I GATC 3 cut(s) 108, 295, 399
LguI GCTCTTC 1 cut(s) 330
LmnI GCTCC 7 cut(s) 155, 177, 260, 272, 287, 345, 380
MaeII ACGT 2 cut(s) 132, 156
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 110, 297, 401
MboI GATC 3 cut(s) 108, 295, 399
MboII GAAGA 4 cut(s) 67, 176, 347, 466
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 5 cut(s) 126, 174, 282, 342, 377
MluCI AATT 1 cut(s) 436
MmeI TCCRAC 2 cut(s) 33, 177
MroNI GCCGGC 2 cut(s) 251, 485
MspI CCGG 2 cut(s) 252, 486
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 4 cut(s) 72, 180, 207, 509
MwoI GCNNNNNNNGC 4 cut(s) 219, 471, 482, 486
NaeI GCCGGC 2 cut(s) 253, 487
NdeII GATC 3 cut(s) 108, 295, 399
NgoMIV GCCGGC 2 cut(s) 251, 485
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 6 cut(s) 16, 151, 256, 268, 289, 490
NmeAIII GCCGAG 3 cut(s) 105, 126, 301
NmuCI GTSAC 1 cut(s) 52
PciSI GCTCTTC 1 cut(s) 330
PcsI WCGNNNNNNNCGW 6 cut(s) 57, 153, 165, 300, 420, 489
PdiI GCCGGC 2 cut(s) 253, 487
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 1 cut(s) 71
Psp124BI GAGCTC 4 cut(s) 126, 174, 282, 342
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 6 cut(s) 16, 151, 256, 268, 289, 490
PspPI GGNCC 3 cut(s) 64, 142, 310
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 62
SacI GAGCTC 4 cut(s) 126, 174, 282, 342
SapI GCTCTTC 1 cut(s) 330
SatI GCNGC 1 cut(s) 70
Sau3AI GATC 3 cut(s) 108, 295, 399
Sau96I GGNCC 3 cut(s) 64, 142, 310
ScrFI CCNGG 1 cut(s) 308
SduI GDGCHC 5 cut(s) 126, 174, 282, 342, 377
SetI ASST 9 cut(s) 66, 86, 126, 135, 159, 174, 282, 342, 348
SinI GGWCC 2 cut(s) 64, 310
SmlI CTYRAG 2 cut(s) 137, 281
SmoI CTYRAG 2 cut(s) 137, 281
Sse9I AATT 1 cut(s) 436
SsiI CCGC 8 cut(s) 33, 67, 70, 205, 313, 330, 358, 394
SstI GAGCTC 4 cut(s) 126, 174, 282, 342
StyD4I CCNGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 409, 493
TaiI ACGT 2 cut(s) 135, 159
TaqI TCGA 7 cut(s) 87, 189, 195, 225, 294, 381, 441
TasI AATT 1 cut(s) 436
TauI GCSGC 1 cut(s) 72
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 205, 383
TspGWI ACGGA 3 cut(s) 27, 103, 367
Tth111I GACNNNGTC 1 cut(s) 62
VpaK11BI GGWCC 2 cut(s) 64, 310
XapI RAATTY 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.