Rh7AG016500

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0007579
Physical Location & Seq
Forward (+)
1099097 .. 1100076
980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG016500.1

Sequence Viewer

Length: 501 bp
ATGGCTTCCAACGGAACCCCAACCACCGCCGTCGCCGACGCCAAACCCGTCGTGACCGACGAGGTCCGCCGCGTCTTCAAGAGCTTCGACGCCAACGGCGACGGCAAGATCTCAACCTCCGAGCTCGGCAACGTCCTCGAGGCCCTCGGCTCCAACGTCCCCGAGGACGAGCTCCAGCGCGTGATGGTCGACCTCGACACCGACCGCGACGGCTTCATCTGCCTCGACGAGTTCAACGCCTTCTGGGTGGCCGGCGACGGCGGCTCCTCGGAGCTCAAGGAGGCCTTCGATCTGTACGACCAGGACCGCAACGGCCTCATCTCCGTCGACGAGCTCCACCTCGTCCTCAACCGGCTTCAGATGAAGTGCACCGTCGAGGATTGCAACCGCATGATCCAGTCCGTTGACGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGAACCACAACTCCAGCAGCGCCGGCACCGTCTCTAACGGCGCCTGA

Protein Analysis

166

Amino Acids

18.03

Weight (kDa)

4.24

Isoelectric Point (pI)

28.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 21 - 48 7.8e-07 EF hand domain
EF-hand_7 PF13499 21 - 81 2.8e-14 EF-hand domain pair
EF-hand_6 PF13405 21 - 50 9.2e-08 EF-hand domain
EF-hand_5 PF13202 24 - 45 9.2e-07 EF hand
EF-hand_7 PF13499 90 - 152 9.9e-15 EF-hand domain pair
EF-hand_1 PF00036 91 - 118 1.1e-06 EF hand domain
EF-hand_6 PF13405 91 - 118 6.4e-07 EF-hand domain
EF-hand_8 PF13833 119 - 155 9.3e-06 EF-hand domain pair
EF-hand_1 PF00036 128 - 154 1.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 479, 494
AccI GTMKAC 2 cut(s) 189, 327
AccII CGCG 3 cut(s) 72, 180, 207
AciI CCGC 7 cut(s) 27, 67, 70, 205, 261, 307, 388
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 4 cut(s) 39, 90, 408, 495
AfaI GTAC 1 cut(s) 296
AgsI TTSAA 3 cut(s) 79, 235, 445
AjnI CCWGG 1 cut(s) 300
AloI GAACNNNNNNTCC 2 cut(s) 449, 481
AluBI AGCT 5 cut(s) 84, 124, 172, 274, 334
AluI AGCT 5 cut(s) 84, 124, 172, 274, 334
Alw21I GWGCWC 5 cut(s) 126, 174, 276, 336, 371
Alw26I GTCTC 1 cut(s) 490
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 2 cut(s) 137, 161
AoxI GGCC 4 cut(s) 141, 249, 282, 313
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 1 cut(s) 471
ApoI RAATTY 1 cut(s) 430
AspLEI GCGC 3 cut(s) 180, 476, 497
AspS9I GGNCC 3 cut(s) 64, 142, 304
AvaI CYCGRG 2 cut(s) 137, 161
AvaII GGWCC 2 cut(s) 64, 304
BaeGI GKGCMC 1 cut(s) 371
BanI GGYRCC 2 cut(s) 479, 494
BanII GRGCYC 4 cut(s) 126, 174, 276, 336
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 5 cut(s) 126, 174, 276, 336, 371
BbvI GCAGC 1 cut(s) 483
BccI CCATC 1 cut(s) 178
BceAI ACGGC 7 cut(s) 14, 112, 118, 226, 274, 328, 430
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 490
BfoI RGCGCY 2 cut(s) 477, 498
BglII AGATCT 1 cut(s) 108
BisI GCNGC 3 cut(s) 70, 262, 472
BlsI GCNGC 3 cut(s) 71, 263, 473
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 64, 304
BmeT110I CYCGRG 2 cut(s) 137, 161
BmgT120I GGNCC 3 cut(s) 64, 142, 304
BmiI GGNNCC 5 cut(s) 16, 151, 265, 481, 496
BmrFI CCNGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 2 cut(s) 158, 451
BpuEI CTTGAG 1 cut(s) 260
BsaHI GRCGYC 4 cut(s) 39, 90, 408, 495
BsaJI CCNNGG 3 cut(s) 145, 162, 267
BsaXI ACNNNNNCTCC 4 cut(s) 248, 278, 449, 479
Bse118I RCCGGY 3 cut(s) 251, 351, 476
Bse1I ACTGG 1 cut(s) 397
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 3 cut(s) 145, 162, 267
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 2 cut(s) 256, 452
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 1 cut(s) 483
Bsh1236I CGCG 3 cut(s) 72, 180, 207
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 4 cut(s) 143, 251, 284, 315
BshNI GGYRCC 2 cut(s) 479, 494
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 5 cut(s) 126, 174, 276, 336, 371
BsiHKCI CYCGRG 2 cut(s) 137, 161
BsiSI CCGG 3 cut(s) 252, 352, 477
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 490
BsmBI CGTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 4 cut(s) 143, 251, 284, 315
BsoBI CYCGRG 2 cut(s) 137, 161
Bsp1286I GDGCHC 5 cut(s) 126, 174, 276, 336, 371
Bsp143I GATC 3 cut(s) 108, 289, 393
BspACI CCGC 7 cut(s) 27, 67, 70, 205, 261, 307, 388
BspANI GGCC 4 cut(s) 143, 251, 284, 315
BspFNI CGCG 3 cut(s) 72, 180, 207
BspLI GGNNCC 5 cut(s) 16, 151, 265, 481, 496
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 479, 494
BsrFI RCCGGY 3 cut(s) 251, 351, 476
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 3 cut(s) 251, 351, 476
BssECI CCNNGG 3 cut(s) 145, 162, 267
BssMI GATC 3 cut(s) 108, 289, 393
BssNI GRCGYC 4 cut(s) 39, 90, 408, 495
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 373, 484
BstACI GRCGYC 4 cut(s) 39, 90, 408, 495
BstC8I GCNNGC 2 cut(s) 253, 478
BstFNI CGCG 3 cut(s) 72, 180, 207
BstH2I RGCGCY 2 cut(s) 477, 498
BstHHI GCGC 3 cut(s) 180, 476, 497
BstKTI GATC 3 cut(s) 111, 292, 396
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 3 cut(s) 108, 289, 393
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 3 cut(s) 219, 261, 477
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 3 cut(s) 72, 180, 207
BstV1I GCAGC 1 cut(s) 483
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 4 cut(s) 143, 251, 284, 315
Cac8I GCNNGC 2 cut(s) 253, 478
CfoI GCGC 3 cut(s) 180, 476, 497
Cfr10I RCCGGY 3 cut(s) 251, 351, 476
Cfr13I GGNCC 3 cut(s) 64, 142, 304
CseI GACGC 4 cut(s) 47, 61, 98, 416
Csp6I GTAC 1 cut(s) 295
CviAII CATG 1 cut(s) 391
CviQI GTAC 1 cut(s) 295
DinI GGCGCC 1 cut(s) 496
DpnI GATC 3 cut(s) 110, 291, 395
DpnII GATC 3 cut(s) 108, 289, 393
EaeI YGGCCR 1 cut(s) 249
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 4 cut(s) 124, 172, 274, 334
Eco147I AGGCCT 1 cut(s) 284
Eco24I GRGCYC 4 cut(s) 126, 174, 276, 336
Eco47I GGWCC 2 cut(s) 64, 304
Eco53kI GAGCTC 4 cut(s) 124, 172, 274, 334
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 2 cut(s) 137, 161
EcoICRI GAGCTC 4 cut(s) 124, 172, 274, 334
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 300
EcoT38I GRGCYC 4 cut(s) 126, 174, 276, 336
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 490
FaeI CATG 1 cut(s) 394
FaiI YATR 1 cut(s) 392
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 390
FblI GTMKAC 2 cut(s) 189, 327
Fnu4HI GCNGC 3 cut(s) 70, 262, 472
FriOI GRGCYC 4 cut(s) 126, 174, 276, 336
Fsp4HI GCNGC 3 cut(s) 70, 262, 472
GlaI GCGC 3 cut(s) 179, 475, 496
GluI GCNGC 3 cut(s) 70, 262, 472
GsuI CTGGAG 2 cut(s) 158, 451
HaeII RGCGCY 2 cut(s) 477, 498
HaeIII GGCC 4 cut(s) 143, 251, 284, 315
HapII CCGG 3 cut(s) 252, 352, 477
HgaI GACGC 4 cut(s) 47, 61, 98, 416
HhaI GCGC 3 cut(s) 180, 476, 497
Hin1I GRCGYC 4 cut(s) 39, 90, 408, 495
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 3 cut(s) 178, 474, 495
HinP1I GCGC 3 cut(s) 178, 474, 495
HincII GTYRAC 3 cut(s) 190, 328, 406
HindII GTYRAC 3 cut(s) 190, 328, 406
HpaII CCGG 3 cut(s) 252, 352, 477
Hpy166II GTNNAC 4 cut(s) 190, 328, 369, 406
Hpy188I TCNGA 3 cut(s) 121, 271, 360
Hpy188III TCNNGA 3 cut(s) 52, 79, 445
Hpy8I GTNNAC 4 cut(s) 190, 328, 369, 406
HpyAV CCTTC 2 cut(s) 250, 295
HpyCH4III ACNGT 2 cut(s) 373, 484
HpyCH4IV ACGT 2 cut(s) 132, 156
HpyCH4V TGCA 2 cut(s) 369, 384
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 261, 477
HpySE526I ACGT 2 cut(s) 132, 156
Hsp92I GRCGYC 4 cut(s) 39, 90, 408, 495
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 3 cut(s) 178, 474, 495
KasI GGCGCC 1 cut(s) 494
KroI GCCGGC 2 cut(s) 251, 476
KroNI GCCGGC 2 cut(s) 253, 478
Kzo9I GATC 3 cut(s) 108, 289, 393
LmnI GCTCC 5 cut(s) 155, 177, 269, 271, 339
LpnPI CCDG 9 cut(s) 188, 229, 265, 287, 314, 365, 410, 481, 490
Lsp1109I GCAGC 1 cut(s) 483
MaeII ACGT 2 cut(s) 132, 156
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 110, 291, 395
MboI GATC 3 cut(s) 108, 289, 393
MboII GAAGA 2 cut(s) 67, 460
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 5 cut(s) 126, 174, 276, 336, 371
MluCI AATT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 495
MmeI TCCRAC 2 cut(s) 33, 177
MroNI GCCGGC 2 cut(s) 251, 476
MspI CCGG 3 cut(s) 252, 352, 477
MspR9I CCNGG 1 cut(s) 302
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 3 cut(s) 72, 180, 207
MwoI GCNNNNNNNGC 3 cut(s) 219, 261, 477
NaeI GCCGGC 2 cut(s) 253, 478
NarI GGCGCC 1 cut(s) 495
NdeII GATC 3 cut(s) 108, 289, 393
NgoMIV GCCGGC 2 cut(s) 251, 476
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 5 cut(s) 16, 151, 265, 481, 496
NmeAIII GCCGAG 2 cut(s) 105, 126
NmuCI GTSAC 1 cut(s) 52
PaeR7I CTCGAG 1 cut(s) 137
PceI AGGCCT 1 cut(s) 284
PcsI WCGNNNNNNNCGW 6 cut(s) 45, 57, 96, 153, 294, 414
PdiI GCCGGC 2 cut(s) 253, 478
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 3 cut(s) 71, 263, 473
PluTI GGCGCC 1 cut(s) 498
Psp124BI GAGCTC 4 cut(s) 126, 174, 276, 336
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 5 cut(s) 16, 151, 265, 481, 496
PspPI GGNCC 3 cut(s) 64, 142, 304
PspXI VCTCGAGB 1 cut(s) 137
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
SacI GAGCTC 4 cut(s) 126, 174, 276, 336
SalI GTCGAC 2 cut(s) 188, 326
SatI GCNGC 3 cut(s) 70, 262, 472
Sau3AI GATC 3 cut(s) 108, 289, 393
Sau96I GGNCC 3 cut(s) 64, 142, 304
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 5 cut(s) 126, 174, 276, 336, 371
SfoI GGCGCC 1 cut(s) 496
Sfr274I CTCGAG 1 cut(s) 137
SgrDI CGTCGACG 1 cut(s) 326
SinI GGWCC 2 cut(s) 64, 304
SlaI CTCGAG 1 cut(s) 137
SmlI CTYRAG 2 cut(s) 137, 275
SmoI CTYRAG 2 cut(s) 137, 275
Sse9I AATT 1 cut(s) 430
SseBI AGGCCT 1 cut(s) 284
SsiI CCGC 7 cut(s) 27, 67, 70, 205, 261, 307, 388
SspDI GGCGCC 1 cut(s) 494
SstI GAGCTC 4 cut(s) 126, 174, 276, 336
StuI AGGCCT 1 cut(s) 284
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 373, 484
TaiI ACGT 2 cut(s) 135, 159
TaqI TCGA 9 cut(s) 87, 138, 189, 195, 225, 288, 327, 375, 435
TaqII GACCGA 1 cut(s) 71
TasI AATT 1 cut(s) 430
TauI GCSGC 2 cut(s) 72, 264
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 471
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 3 cut(s) 205, 377, 470
TspGWI ACGGA 3 cut(s) 27, 313, 391
Tth111I GACNNNGTC 1 cut(s) 62
VneI GTGCAC 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 64, 304
XapI RAATTY 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 137
XmiI GTMKAC 2 cut(s) 189, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.