Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0007579

Physical Location & Seq

Genomic Coordinates Reverse (-)

36066604 .. 36067131

Gene Structure 528 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1231000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 528 bp

ATGGCAACCAACTGTGCTCCAGCCTCCACCGACCCCAAACCCGCCGTCACCGACCCCAAGCCCGCCGTCACCGACGAGGTCCGGAAGGTCTTCAAAAGCTTCGACTCCAACGGCGATGGGAAGATCTCCGTCAACGAGCTCGGCAACGTCCTCACGGCCCTCGGCTCCACCGTCTCCGAAGACGAGCTCAAGCGCTTCATGGTCGATCTCGACACCGACAACGACGGCTTCATTTGCCTCGACGAGTTCAACGCCTTCTGGGTCGCCGGCCCCAAGGACGGCGGCGTCACCGAGCTCAAAGACGCCTTCGATCTCTACGACCAGGATCGGAACGGCCTCATCTCCGCCAACGAGCTCCACCTGGTGCTCAACCGCCTCAACATGGCCTGCACGATCGAGGACTGCCATCGGATGATCAAGACCGTCGATGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGGGACACAAAGCCTCCTCCGACGGCACCAATGCCGCCAACCCTGCCGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0016020 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7

Protein Analysis

176

Amino Acids

18.91

Weight (kDa)

4.45

Isoelectric Point (pI)

28.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_1	PF00036	26 - 53	2.3e-07	EF hand domain
EF-hand_7	PF13499	26 - 86	4.4e-15	EF-hand domain pair
EF-hand_6	PF13405	26 - 55	2.8e-08	EF-hand domain
EF-hand_5	PF13202	28 - 50	1.2e-06	EF hand
EF-hand_7	PF13499	97 - 159	5.7e-13	EF-hand domain pair
EF-hand_1	PF00036	98 - 125	5.7e-06	EF hand domain
EF-hand_6	PF13405	98 - 126	1.4e-06	EF-hand domain
EF-hand_1	PF00036	135 - 160	1.6e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007579)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18210 AT1G18210 AT1G73630
fragaria_vesca	FvH4_5g17240
malus_domestica	MD03G1137400.v1.1 MD06G1216800.v1.1 MD06G1231000.v1.1 MD13G1184700.v1.1
prunus_persica	Prupe.1G192900_v2.0.a1 Prupe.3G199600_v2.0.a1 Prupe.5G232300_v2.0.a1 Prupe.7G023700_v2.0.a1
pyrus_communis	pycom14g19650 pycom14g19660
rosa_chinensis	RchiOBHm_Chr7g0178551
rosa_laevigata	RLG00000005438
rosa_multiflora	Rmu_co8293555.1_g000001 Rmu_sc0012505.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274820
rosa_rugosa	Rorug06G0417900
rosa_samantha	Rh7AG016500 Rh7BG016000 Rh7DG016600
rosa_wichuraiana	Rw7G001310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	284
AccB1I	GGYRCC	1 cut(s)	500
AccIII	TCCGGA	1 cut(s)	81
AciI	CCGC	7 cut(s)	42, 63, 282, 345, 373, 510, 522
AclWI	GGATC	1 cut(s)	333
AcsI	RAATTY	1 cut(s)	451
AcyI	GRCGYC	2 cut(s)	285, 303
AdeI	CACNNNGTG	1 cut(s)	364
AfeI	AGCGCT	1 cut(s)	194
AfiI	CCNNNNNNNGG	2 cut(s)	278, 382
AgsI	TTSAA	3 cut(s)	94, 250, 466
AjnI	CCWGG	2 cut(s)	321, 360
AluBI	AGCT	5 cut(s)	99, 139, 187, 295, 355
AluI	AGCT	5 cut(s)	99, 139, 187, 295, 355
Alw21I	GWGCWC	6 cut(s)	19, 141, 189, 297, 357, 369
Alw26I	GTCTC	1 cut(s)	178
AlwI	GGATC	1 cut(s)	333
Aor13HI	TCCGGA	1 cut(s)	81
Aor51HI	AGCGCT	1 cut(s)	194
AoxI	GGCC	4 cut(s)	156, 268, 334, 384
ApoI	RAATTY	1 cut(s)	451
ArsI	GACNNNNNNTTYG	2 cut(s)	30, 62
Asp700I	GAANNNNTTC	1 cut(s)	89
AspLEI	GCGC	1 cut(s)	195
AspS9I	GGNCC	3 cut(s)	79, 157, 269
AsuHPI	GGTGA	3 cut(s)	40, 61, 280
AvaII	GGWCC	1 cut(s)	79
BanI	GGYRCC	1 cut(s)	500
BanII	GRGCYC	4 cut(s)	141, 189, 297, 357
BbsI	GAAGAC	2 cut(s)	82, 186
Bbv12I	GWGCWC	6 cut(s)	19, 141, 189, 297, 357, 369
BccI	CCATC	3 cut(s)	110, 414, 469
BceAI	ACGGC	9 cut(s)	29, 50, 127, 171, 241, 295, 349, 451, 514
BciT130I	CCWGG	2 cut(s)	323, 362
BclI	TGATCA	1 cut(s)	414
BcoDI	GTCTC	1 cut(s)	178
BfaI	CTAG	1 cut(s)	526
BfoI	RGCGCY	1 cut(s)	196
BglII	AGATCT	1 cut(s)	123
BisI	GCNGC	3 cut(s)	283, 510, 522
BlsI	GCNGC	3 cut(s)	284, 511, 523
Bme1390I	CCNGG	2 cut(s)	323, 362
Bme18I	GGWCC	1 cut(s)	79
BmgT120I	GGNCC	3 cut(s)	79, 157, 269
BmiI	GGNNCC	3 cut(s)	166, 271, 502
BmrFI	CCNGG	2 cut(s)	323, 362
BmsI	GCATC	1 cut(s)	418
BpiI	GAAGAC	2 cut(s)	82, 186
BpuEI	CTTGAG	1 cut(s)	173
BsaHI	GRCGYC	2 cut(s)	285, 303
BsaJI	CCNNGG	2 cut(s)	160, 273
BsaWI	WCCGGW	1 cut(s)	81
BsaXI	ACNNNNNCTCC	2 cut(s)	473, 503
Bsc4I	CCNNNNNNNGG	2 cut(s)	278, 382
Bse118I	RCCGGY	1 cut(s)	266
BseAI	TCCGGA	1 cut(s)	81
BseBI	CCWGG	2 cut(s)	323, 362
BseDI	CCNNGG	2 cut(s)	160, 273
BseGI	GGATG	1 cut(s)	417
BseLI	CCNNNNNNNGG	2 cut(s)	278, 382
BseRI	GAGGAG	2 cut(s)	473, 481
BsgI	GTGCAG	1 cut(s)	373
Bsh1285I	CGRYCG	1 cut(s)	396
BshFI	GGCC	4 cut(s)	158, 270, 336, 386
BshNI	GGYRCC	1 cut(s)	500
BsiEI	CGRYCG	1 cut(s)	396
BsiHKAI	GWGCWC	6 cut(s)	19, 141, 189, 297, 357, 369
BsiSI	CCGG	2 cut(s)	82, 267
BslFI	GGGAC	1 cut(s)	492
BslI	CCNNNNNNNGG	2 cut(s)	278, 382
BsmAI	GTCTC	1 cut(s)	178
BsmBI	CGTCTC	1 cut(s)	178
BsmFI	GGGAC	1 cut(s)	492
BsnI	GGCC	4 cut(s)	158, 270, 336, 386
Bsp1286I	GDGCHC	6 cut(s)	19, 141, 189, 297, 357, 369
Bsp13I	TCCGGA	1 cut(s)	81
Bsp143I	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
BspACI	CCGC	7 cut(s)	42, 63, 282, 345, 373, 510, 522
BspANI	GGCC	4 cut(s)	158, 270, 336, 386
BspEI	TCCGGA	1 cut(s)	81
BspLI	GGNNCC	3 cut(s)	166, 271, 502
BspPI	GGATC	1 cut(s)	333
BspT107I	GGYRCC	1 cut(s)	500
BsrFI	RCCGGY	1 cut(s)	266
BssAI	RCCGGY	1 cut(s)	266
BssECI	CCNNGG	2 cut(s)	160, 273
BssMI	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
BssNI	GRCGYC	2 cut(s)	285, 303
BssT1I	CCWWGG	1 cut(s)	273
Bst2UI	CCWGG	2 cut(s)	323, 362
Bst4CI	ACNGT	3 cut(s)	14, 172, 424
BstACI	GRCGYC	2 cut(s)	285, 303
BstC8I	GCNNGC	3 cut(s)	63, 268, 388
BstF5I	GGATG	1 cut(s)	417
BstH2I	RGCGCY	1 cut(s)	196
BstHHI	GCGC	1 cut(s)	195
BstKTI	GATC	6 cut(s)	126, 208, 313, 328, 396, 417
BstMAI	GTCTC	1 cut(s)	178
BstMBI	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
BstMCI	CGRYCG	1 cut(s)	396
BstMWI	GCNNNNNNNGC	2 cut(s)	234, 518
BstNI	CCWGG	2 cut(s)	323, 362
BstSCI	CCNGG	2 cut(s)	321, 360
BstV2I	GAAGAC	2 cut(s)	82, 186
BstX2I	RGATCY	1 cut(s)	123
BstYI	RGATCY	1 cut(s)	123
BsuRI	GGCC	4 cut(s)	158, 270, 336, 386
BtgZI	GCGATG	1 cut(s)	129
BtsCI	GGATG	1 cut(s)	417
Cac8I	GCNNGC	3 cut(s)	63, 268, 388
CfoI	GCGC	1 cut(s)	195
Cfr10I	RCCGGY	1 cut(s)	266
Cfr13I	GGNCC	3 cut(s)	79, 157, 269
CseI	GACGC	2 cut(s)	274, 311
CsiI	ACCWGGT	1 cut(s)	360
CviAII	CATG	2 cut(s)	199, 382
DpnI	GATC	6 cut(s)	125, 207, 312, 327, 395, 416
DpnII	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
DraIII	CACNNNGTG	1 cut(s)	364
DrdI	GACNNNNNNGTC	1 cut(s)	284
DseDI	GACNNNNNNGTC	1 cut(s)	284
EciI	GGCGGA	1 cut(s)	334
Ecl136II	GAGCTC	4 cut(s)	139, 187, 295, 355
Eco130I	CCWWGG	1 cut(s)	273
Eco24I	GRGCYC	4 cut(s)	141, 189, 297, 357
Eco47I	GGWCC	1 cut(s)	79
Eco47III	AGCGCT	1 cut(s)	194
Eco53kI	GAGCTC	4 cut(s)	139, 187, 295, 355
EcoICRI	GAGCTC	4 cut(s)	139, 187, 295, 355
EcoRII	CCWGG	2 cut(s)	321, 360
EcoT14I	CCWWGG	1 cut(s)	273
EcoT38I	GRGCYC	4 cut(s)	141, 189, 297, 357
ErhI	CCWWGG	1 cut(s)	273
Esp3I	CGTCTC	1 cut(s)	178
FaeI	CATG	2 cut(s)	202, 385
FaiI	YATR	2 cut(s)	200, 383
FaqI	GGGAC	1 cut(s)	492
FatI	CATG	2 cut(s)	198, 381
FauI	CCCGC	2 cut(s)	49, 70
FbaI	TGATCA	1 cut(s)	414
Fnu4HI	GCNGC	3 cut(s)	283, 510, 522
FokI	GGATG	1 cut(s)	424
FriOI	GRGCYC	4 cut(s)	141, 189, 297, 357
Fsp4HI	GCNGC	3 cut(s)	283, 510, 522
FspBI	CTAG	1 cut(s)	526
GlaI	GCGC	1 cut(s)	194
GluI	GCNGC	3 cut(s)	283, 510, 522
HaeII	RGCGCY	1 cut(s)	196
HaeIII	GGCC	4 cut(s)	158, 270, 336, 386
HapII	CCGG	2 cut(s)	82, 267
HgaI	GACGC	2 cut(s)	274, 311
HhaI	GCGC	1 cut(s)	195
Hin1I	GRCGYC	2 cut(s)	285, 303
Hin1II	CATG	2 cut(s)	202, 385
Hin6I	GCGC	1 cut(s)	193
HinP1I	GCGC	1 cut(s)	193
HincII	GTYRAC	1 cut(s)	133
HindII	GTYRAC	1 cut(s)	133
HindIII	AAGCTT	1 cut(s)	97
HinfI	GANTC	1 cut(s)	104
HpaII	CCGG	2 cut(s)	82, 267
HphI	GGTGA	3 cut(s)	40, 61, 280
Hpy166II	GTNNAC	1 cut(s)	133
Hpy188I	TCNGA	4 cut(s)	178, 330, 411, 496
Hpy188III	TCNNGA	4 cut(s)	82, 209, 418, 466
Hpy8I	GTNNAC	1 cut(s)	133
Hpy99I	CGWCG	7 cut(s)	77, 227, 245, 428, 437, 443, 500
HpyAV	CCTTC	3 cut(s)	79, 265, 316
HpyCH4III	ACNGT	3 cut(s)	14, 172, 424
HpyCH4IV	ACGT	1 cut(s)	147
HpyCH4V	TGCA	1 cut(s)	390
HpyF10VI	GCNNNNNNNGC	2 cut(s)	234, 518
HpySE526I	ACGT	1 cut(s)	147
Hsp92I	GRCGYC	2 cut(s)	285, 303
Hsp92II	CATG	2 cut(s)	202, 385
HspAI	GCGC	1 cut(s)	193
Kpn2I	TCCGGA	1 cut(s)	81
KroI	GCCGGC	1 cut(s)	266
KroNI	GCCGGC	1 cut(s)	268
Ksp22I	TGATCA	1 cut(s)	414
Kzo9I	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
LmnI	GCTCC	3 cut(s)	22, 170, 360
LpnPI	CCDG	9 cut(s)	33, 95, 244, 280, 308, 335, 347, 374, 400
LweI	GCATC	1 cut(s)	418
MabI	ACCWGGT	1 cut(s)	360
MaeI	CTAG	1 cut(s)	526
MaeII	ACGT	1 cut(s)	147
MaeIII	GTNAC	3 cut(s)	46, 67, 286
MalI	GATC	6 cut(s)	125, 207, 312, 327, 395, 416
MboI	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
MboII	GAAGA	4 cut(s)	82, 133, 191, 481
MflI	RGATCY	1 cut(s)	123
MhlI	GDGCHC	6 cut(s)	19, 141, 189, 297, 357, 369
MluCI	AATT	1 cut(s)	451
MlyI	GAGTC	1 cut(s)	98
MmeI	TCCRAC	2 cut(s)	132, 519
MroI	TCCGGA	1 cut(s)	81
MroNI	GCCGGC	1 cut(s)	266
MroXI	GAANNNNTTC	1 cut(s)	89
MspI	CCGG	2 cut(s)	82, 267
MspR9I	CCNGG	2 cut(s)	323, 362
MvaI	CCWGG	2 cut(s)	323, 362
MwoI	GCNNNNNNNGC	2 cut(s)	234, 518
NaeI	GCCGGC	1 cut(s)	268
NdeII	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
NgoMIV	GCCGGC	1 cut(s)	266
NlaIII	CATG	2 cut(s)	202, 385
NlaIV	GGNNCC	3 cut(s)	166, 271, 502
NmeAIII	GCCGAG	2 cut(s)	120, 141
NmuCI	GTSAC	3 cut(s)	46, 67, 286
PcsI	WCGNNNNNNNCGW	2 cut(s)	168, 315
PdiI	GCCGGC	1 cut(s)	268
PdmI	GAANNNNTTC	1 cut(s)	89
PflFI	GACNNNGTC	1 cut(s)	77
PkrI	GCNGC	3 cut(s)	284, 511, 523
Ple19I	CGATCG	1 cut(s)	396
PleI	GAGTC	1 cut(s)	98
PpsI	GAGTC	1 cut(s)	98
Psp124BI	GAGCTC	4 cut(s)	141, 189, 297, 357
Psp6I	CCWGG	2 cut(s)	321, 360
PspGI	CCWGG	2 cut(s)	321, 360
PspN4I	GGNNCC	3 cut(s)	166, 271, 502
PspPI	GGNCC	3 cut(s)	79, 157, 269
PsuI	RGATCY	1 cut(s)	123
PsyI	GACNNNGTC	1 cut(s)	77
PvuI	CGATCG	1 cut(s)	396
SacI	GAGCTC	4 cut(s)	141, 189, 297, 357
SatI	GCNGC	3 cut(s)	283, 510, 522
Sau3AI	GATC	6 cut(s)	123, 205, 310, 325, 393, 414
Sau96I	GGNCC	3 cut(s)	79, 157, 269
SchI	GAGTC	1 cut(s)	98
ScrFI	CCNGG	2 cut(s)	323, 362
SduI	GDGCHC	6 cut(s)	19, 141, 189, 297, 357, 369
SetI	ASST	9 cut(s)	81, 90, 101, 141, 150, 189, 297, 357, 363
SexAI	ACCWGGT	1 cut(s)	360
SfaNI	GCATC	1 cut(s)	418
SinI	GGWCC	1 cut(s)	79
SmlI	CTYRAG	1 cut(s)	188
SmoI	CTYRAG	1 cut(s)	188
Sse9I	AATT	1 cut(s)	451
SsiI	CCGC	7 cut(s)	42, 63, 282, 345, 373, 510, 522
SspMI	CTAG	1 cut(s)	526
SstI	GAGCTC	4 cut(s)	141, 189, 297, 357
StyD4I	CCNGG	2 cut(s)	321, 360
StyI	CCWWGG	1 cut(s)	273
TaaI	ACNGT	3 cut(s)	14, 172, 424
TaiI	ACGT	1 cut(s)	150
TaqI	TCGA	8 cut(s)	102, 204, 210, 240, 309, 396, 426, 456
TasI	AATT	1 cut(s)	451
TauI	GCSGC	3 cut(s)	285, 512, 524
TseFI	GTSAC	3 cut(s)	46, 67, 286
Tsp45I	GTSAC	3 cut(s)	46, 67, 286
TspDTI	ATGAA	2 cut(s)	187, 220
TspGWI	ACGGA	1 cut(s)	118
Tth111I	GACNNNGTC	1 cut(s)	77
VpaK11BI	GGWCC	1 cut(s)	79
XapI	RAATTY	1 cut(s)	451
XmnI	GAANNNNTTC	1 cut(s)	89
XspI	CTAG	1 cut(s)	526

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.