MD06G1216800.v1.1

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007579
Physical Location & Seq
Reverse (-)
34908520 .. 34908867
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1216800.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCAACCAGCGGAGCCCTAACCTCGACAGGCCCCAAACCCGTAGTCACCGACGAGGTCCTGAAAGTCTTCAAAAGCTTCGACGCCAGCGGCGATGGCAAGATCTCCGTCAACGAGCTCGGCAACGACCTCAAGGCCCTCGGCTTCAACGTCTCAAACGACAAGCTCAAGCGTGTTAAGGTCGATCTCGACACCGACAATGATGGCTTCATTTGCCTTGACGAGTTCAACGCCTTCTGGGTCTCCTCCAGCTTAAACAGGAGTCCAAAACCTACGACCCGATATCTCACTTCTATAGCTATATGTCAGGCACGAGACGCAAGACTGGTTGAAGGTCCACATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

116

Amino Acids

12.45

Weight (kDa)

5.74

Isoelectric Point (pI)

14.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 21 - 79 2.4e-10 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 12, 90
AcyI GRCGYC 1 cut(s) 84
AgsI TTSAA 4 cut(s) 73, 148, 229, 332
AluBI AGCT 5 cut(s) 78, 118, 166, 252, 299
AluI AGCT 5 cut(s) 78, 118, 166, 252, 299
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 3 cut(s) 157, 247, 309
AoxI GGCC 2 cut(s) 31, 135
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
Asp700I GAANNNNTTC 1 cut(s) 68
AspS9I GGNCC 4 cut(s) 32, 58, 136, 335
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 2 cut(s) 58, 335
BanII GRGCYC 2 cut(s) 19, 120
BauI CACGAG 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 120
BccI CCATC 2 cut(s) 89, 197
BcoDI GTCTC 3 cut(s) 157, 247, 309
BfmI CTRYAG 1 cut(s) 294
BglII AGATCT 1 cut(s) 102
BisI GCNGC 1 cut(s) 91
BlsI GCNGC 1 cut(s) 92
Bme18I GGWCC 2 cut(s) 58, 335
BmgT120I GGNCC 4 cut(s) 32, 58, 136, 335
BmiI GGNNCC 2 cut(s) 16, 34
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 232
BpuEI CTTGAG 2 cut(s) 116, 152
BsaHI GRCGYC 1 cut(s) 84
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 139
Bse1I ACTGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 139
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 235
BshFI GGCC 2 cut(s) 33, 137
BsiHKAI GWGCWC 1 cut(s) 120
BsmAI GTCTC 3 cut(s) 157, 247, 309
BsmBI CGTCTC 2 cut(s) 157, 309
BsnI GGCC 2 cut(s) 33, 137
Bso31I GGTCTC 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 19, 120
Bsp143I GATC 2 cut(s) 102, 184
BspACI CCGC 2 cut(s) 12, 90
BspANI GGCC 2 cut(s) 33, 137
BspLI GGNNCC 2 cut(s) 16, 34
BspTNI GGTCTC 1 cut(s) 247
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 102, 184
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 312
Bst2BI CACGAG 1 cut(s) 312
BstACI GRCGYC 1 cut(s) 84
BstC8I GCNNGC 1 cut(s) 88
BstKTI GATC 2 cut(s) 105, 187
BstMAI GTCTC 3 cut(s) 157, 247, 309
BstMBI GATC 2 cut(s) 102, 184
BstMWI GCNNNNNNNGC 3 cut(s) 96, 213, 317
BstSFI CTRYAG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 2 cut(s) 33, 137
BtgZI GCGATG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 88
Cfr13I GGNCC 4 cut(s) 32, 58, 136, 335
CseI GACGC 2 cut(s) 92, 326
DpnI GATC 2 cut(s) 104, 186
DpnII GATC 2 cut(s) 102, 184
Ecl136II GAGCTC 1 cut(s) 118
Eco24I GRGCYC 2 cut(s) 19, 120
Eco31I GGTCTC 1 cut(s) 247
Eco32I GATATC 1 cut(s) 284
Eco47I GGWCC 2 cut(s) 58, 335
Eco53kI GAGCTC 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 3 cut(s) 32, 58, 136
EcoRV GATATC 1 cut(s) 284
EcoT38I GRGCYC 2 cut(s) 19, 120
Esp3I CGTCTC 2 cut(s) 157, 309
FaiI YATR 4 cut(s) 296, 302, 304, 342
Fnu4HI GCNGC 1 cut(s) 91
FriOI GRGCYC 2 cut(s) 19, 120
Fsp4HI GCNGC 1 cut(s) 91
GluI GCNGC 1 cut(s) 91
GsuI CTGGAG 1 cut(s) 232
HaeIII GGCC 2 cut(s) 33, 137
HgaI GACGC 2 cut(s) 92, 326
Hin1I GRCGYC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 1 cut(s) 262
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 2 cut(s) 112, 338
Hpy188III TCNNGA 2 cut(s) 61, 188
Hpy8I GTNNAC 2 cut(s) 112, 338
Hpy99I CGWCG 2 cut(s) 56, 86
HpyAV CCTTC 2 cut(s) 244, 326
HpyCH4IV ACGT 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 213, 317
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 1 cut(s) 84
Kzo9I GATC 2 cut(s) 102, 184
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 9 cut(s) 15, 22, 74, 100, 223, 244, 262, 293, 311
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 104, 186
MboI GATC 2 cut(s) 102, 184
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 19, 120
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 5 cut(s) 34, 49, 140, 149, 256
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 2 cut(s) 177, 254
MspA1I CMGCKG 2 cut(s) 12, 90
MwoI GCNNNNNNNGC 3 cut(s) 96, 213, 317
NdeII GATC 2 cut(s) 102, 184
NlaIV GGNNCC 2 cut(s) 16, 34
NmeAIII GCCGAG 2 cut(s) 99, 120
NmuCI GTSAC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 147, 156
PdmI GAANNNNTTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 58
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 16, 34
PspPI GGNCC 4 cut(s) 32, 58, 136, 335
PspPPI RGGWCCY 1 cut(s) 58
PsuI RGATCY 1 cut(s) 102
PsyI GACNNNGTC 1 cut(s) 56
SacI GAGCTC 1 cut(s) 120
SaqAI TTAA 2 cut(s) 177, 254
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 2 cut(s) 102, 184
Sau96I GGNCC 4 cut(s) 32, 58, 136, 335
SchI GAGTC 1 cut(s) 271
SduI GDGCHC 2 cut(s) 19, 120
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 2 cut(s) 58, 335
SmlI CTYRAG 2 cut(s) 131, 167
SmoI CTYRAG 2 cut(s) 131, 167
SsiI CCGC 2 cut(s) 12, 90
SstI GAGCTC 1 cut(s) 120
TaiI ACGT 1 cut(s) 153
TaqI TCGA 4 cut(s) 26, 81, 183, 189
TauI GCSGC 1 cut(s) 93
Tru1I TTAA 2 cut(s) 177, 254
Tru9I TTAA 2 cut(s) 177, 254
TseFI GTSAC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 97
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 58, 335
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.