MD13G1184700.v1.1

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007579
Physical Location & Seq
Forward (+)
15689364 .. 15689843
480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1184700.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCAACCAACGGAGCCCCAACCTCGACCGGCCCCAAACCCGTAGTCACCGACGAGGTCCAGAAGGTCTTCAAAGGCTTCGACGCCAGCGGCGATGGCAAGATCTCCGTCAACGAGCTCGGCAACGACCTCAAGGCCCTCGGCTCCAACGTCTCCGGCGACAAGCTCAAGCGCGTCATGGTCGATCTCGACACCGACAACGATGGCTTCATTTGCCTCGACGAGTTCAACGCCTTCTGGGTCTCCGACTCCAAGGACGGCGGCGCCGCTGAGCTCAAAGACGCCTTCGATCTCTACGACCAGGACCGCAACGGCCTCATCTCCACCAACGAACTCCACCTGGTGCTCAGCCGCCTCAACTTGGCCTGCACGGTCGAGGATTGCAACCGGATGATCAAGTCCGTCGATGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGGGACACAGCGTCTCCACCAACTGA

Protein Analysis

160

Amino Acids

17.16

Weight (kDa)

4.43

Isoelectric Point (pI)

13.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 19 - 79 3.4e-10 EF-hand domain pair
EF-hand_1 PF00036 91 - 118 5.6e-06 EF hand domain
EF-hand_7 PF13499 91 - 152 2.4e-13 EF-hand domain pair
EF-hand_6 PF13405 91 - 118 2e-06 EF-hand domain
EF-hand_1 PF00036 128 - 153 2.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 263
AccII CGCG 1 cut(s) 174
AciI CCGC 5 cut(s) 90, 261, 267, 307, 352
AcsI RAATTY 1 cut(s) 430
AcyI GRCGYC 3 cut(s) 84, 264, 282
AdeI CACNNNGTG 1 cut(s) 343
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 3 cut(s) 73, 229, 445
AhdI GACNNNNNGTC 1 cut(s) 464
AjnI CCWGG 2 cut(s) 300, 339
AluBI AGCT 3 cut(s) 118, 166, 274
AluI AGCT 3 cut(s) 118, 166, 274
Alw21I GWGCWC 3 cut(s) 120, 276, 348
Alw26I GTCTC 3 cut(s) 157, 247, 472
AoxI GGCC 4 cut(s) 31, 135, 313, 363
ApoI RAATTY 1 cut(s) 430
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 2 cut(s) 174, 266
AspS9I GGNCC 4 cut(s) 32, 58, 136, 304
AsuHPI GGTGA 1 cut(s) 40
AvaII GGWCC 2 cut(s) 58, 304
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 3 cut(s) 19, 120, 276
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 3 cut(s) 120, 276, 348
BccI CCATC 3 cut(s) 89, 197, 448
BceAI ACGGC 3 cut(s) 274, 328, 430
BciT130I CCWGG 2 cut(s) 302, 341
BclI TGATCA 1 cut(s) 393
BcoDI GTCTC 3 cut(s) 157, 247, 472
BfoI RGCGCY 1 cut(s) 267
BglII AGATCT 1 cut(s) 102
BisI GCNGC 4 cut(s) 91, 262, 267, 352
BlpI GCTNAGC 2 cut(s) 270, 347
BlsI GCNGC 4 cut(s) 92, 263, 268, 353
Bme1390I CCNGG 2 cut(s) 302, 341
Bme18I GGWCC 2 cut(s) 58, 304
BmeRI GACNNNNNGTC 1 cut(s) 464
BmgT120I GGNCC 4 cut(s) 32, 58, 136, 304
BmiI GGNNCC 4 cut(s) 16, 34, 145, 265
BmrFI CCNGG 2 cut(s) 302, 341
BmsI GCATC 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 61
Bpu1102I GCTNAGC 2 cut(s) 270, 347
BpuEI CTTGAG 2 cut(s) 116, 152
BsaHI GRCGYC 3 cut(s) 84, 264, 282
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 139, 252
BsaWI WCCGGW 1 cut(s) 387
BsaXI ACNNNNNCTCC 1 cut(s) 452
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse118I RCCGGY 1 cut(s) 29
BseBI CCWGG 2 cut(s) 302, 341
BseDI CCNNGG 2 cut(s) 139, 252
BseGI GGATG 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 261, 361
BseRI GAGGAG 1 cut(s) 452
BsgI GTGCAG 1 cut(s) 352
Bsh1236I CGCG 1 cut(s) 174
Bsh1285I CGRYCG 2 cut(s) 30, 375
BshFI GGCC 4 cut(s) 33, 137, 315, 365
BshNI GGYRCC 1 cut(s) 263
BsiEI CGRYCG 2 cut(s) 30, 375
BsiHKAI GWGCWC 3 cut(s) 120, 276, 348
BsiSI CCGG 3 cut(s) 30, 156, 388
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 3 cut(s) 157, 247, 472
BsmBI CGTCTC 2 cut(s) 157, 472
BsmFI GGGAC 1 cut(s) 471
BsnI GGCC 4 cut(s) 33, 137, 315, 365
Bso31I GGTCTC 1 cut(s) 247
Bsp1286I GDGCHC 4 cut(s) 19, 120, 276, 348
Bsp143I GATC 4 cut(s) 102, 184, 289, 393
Bsp1720I GCTNAGC 2 cut(s) 270, 347
BspACI CCGC 5 cut(s) 90, 261, 267, 307, 352
BspANI GGCC 4 cut(s) 33, 137, 315, 365
BspCNI CTCAG 2 cut(s) 262, 360
BspFNI CGCG 1 cut(s) 174
BspLI GGNNCC 4 cut(s) 16, 34, 145, 265
BspT107I GGYRCC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 139, 252
BssMI GATC 4 cut(s) 102, 184, 289, 393
BssNI GRCGYC 3 cut(s) 84, 264, 282
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 2 cut(s) 302, 341
Bst4CI ACNGT 1 cut(s) 373
BstACI GRCGYC 3 cut(s) 84, 264, 282
BstC8I GCNNGC 2 cut(s) 88, 367
BstDEI CTNAG 2 cut(s) 270, 347
BstF5I GGATG 1 cut(s) 396
BstFNI CGCG 1 cut(s) 174
BstH2I RGCGCY 1 cut(s) 267
BstHHI GCGC 2 cut(s) 174, 266
BstKTI GATC 4 cut(s) 105, 187, 292, 396
BstMAI GTCTC 3 cut(s) 157, 247, 472
BstMBI GATC 4 cut(s) 102, 184, 289, 393
BstMCI CGRYCG 2 cut(s) 30, 375
BstMWI GCNNNNNNNGC 2 cut(s) 96, 213
BstNI CCWGG 2 cut(s) 302, 341
BstSCI CCNGG 2 cut(s) 300, 339
BstUI CGCG 1 cut(s) 174
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 4 cut(s) 33, 137, 315, 365
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 1 cut(s) 396
Cac8I GCNNGC 2 cut(s) 88, 367
CfoI GCGC 2 cut(s) 174, 266
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 32, 58, 136, 304
CseI GACGC 4 cut(s) 92, 163, 290, 454
CsiI ACCWGGT 1 cut(s) 339
CviAII CATG 1 cut(s) 178
DdeI CTNAG 2 cut(s) 270, 347
DinI GGCGCC 1 cut(s) 265
DpnI GATC 4 cut(s) 104, 186, 291, 395
DpnII GATC 4 cut(s) 102, 184, 289, 393
DraIII CACNNNGTG 1 cut(s) 343
DriI GACNNNNNGTC 1 cut(s) 464
Eam1105I GACNNNNNGTC 1 cut(s) 464
Ecl136II GAGCTC 2 cut(s) 118, 274
Eco130I CCWWGG 1 cut(s) 252
Eco24I GRGCYC 3 cut(s) 19, 120, 276
Eco31I GGTCTC 1 cut(s) 247
Eco47I GGWCC 2 cut(s) 58, 304
Eco53kI GAGCTC 2 cut(s) 118, 274
EcoICRI GAGCTC 2 cut(s) 118, 274
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 300, 339
EcoT14I CCWWGG 1 cut(s) 252
EcoT38I GRGCYC 3 cut(s) 19, 120, 276
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 252
Esp3I CGTCTC 2 cut(s) 157, 472
FaeI CATG 1 cut(s) 181
FaiI YATR 1 cut(s) 179
FaqI GGGAC 1 cut(s) 471
FatI CATG 1 cut(s) 177
FbaI TGATCA 1 cut(s) 393
Fnu4HI GCNGC 4 cut(s) 91, 262, 267, 352
FokI GGATG 1 cut(s) 403
FriOI GRGCYC 3 cut(s) 19, 120, 276
Fsp4HI GCNGC 4 cut(s) 91, 262, 267, 352
GlaI GCGC 2 cut(s) 173, 265
GluI GCNGC 4 cut(s) 91, 262, 267, 352
HaeII RGCGCY 1 cut(s) 267
HaeIII GGCC 4 cut(s) 33, 137, 315, 365
HapII CCGG 3 cut(s) 30, 156, 388
HgaI GACGC 4 cut(s) 92, 163, 290, 454
HhaI GCGC 2 cut(s) 174, 266
Hin1I GRCGYC 3 cut(s) 84, 264, 282
Hin1II CATG 1 cut(s) 181
Hin6I GCGC 2 cut(s) 172, 264
HinP1I GCGC 2 cut(s) 172, 264
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 1 cut(s) 248
HpaII CCGG 3 cut(s) 30, 156, 388
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 61, 188, 445
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 6 cut(s) 56, 86, 224, 407, 416, 422
HpyAV CCTTC 3 cut(s) 58, 244, 295
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 2 cut(s) 369, 384
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 213
HpyF3I CTNAG 2 cut(s) 270, 347
HpySE526I ACGT 1 cut(s) 150
Hsp92I GRCGYC 3 cut(s) 84, 264, 282
Hsp92II CATG 1 cut(s) 181
HspAI GCGC 2 cut(s) 172, 264
KasI GGCGCC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 4 cut(s) 102, 184, 289, 393
LmnI GCTCC 2 cut(s) 14, 149
LweI GCATC 1 cut(s) 397
MabI ACCWGGT 1 cut(s) 339
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 1 cut(s) 46
MalI GATC 4 cut(s) 104, 186, 291, 395
MboI GATC 4 cut(s) 102, 184, 289, 393
MboII GAAGA 2 cut(s) 61, 460
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 4 cut(s) 19, 120, 276, 348
MluCI AATT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 2 cut(s) 171, 270
MnlI CCTC 9 cut(s) 34, 49, 140, 149, 227, 326, 365, 370, 430
MroXI GAANNNNTTC 1 cut(s) 68
MspA1I CMGCKG 2 cut(s) 90, 269
MspI CCGG 3 cut(s) 30, 156, 388
MspR9I CCNGG 2 cut(s) 302, 341
MvaI CCWGG 2 cut(s) 302, 341
MvnI CGCG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 96, 213
NarI GGCGCC 1 cut(s) 264
NdeII GATC 4 cut(s) 102, 184, 289, 393
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 4 cut(s) 16, 34, 145, 265
NmeAIII GCCGAG 2 cut(s) 99, 120
NmuCI GTSAC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 4 cut(s) 90, 147, 156, 294
PdmI GAANNNNTTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 56
PkrI GCNGC 4 cut(s) 92, 263, 268, 353
PleI GAGTC 1 cut(s) 242
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 242
Psp124BI GAGCTC 2 cut(s) 120, 276
Psp6I CCWGG 2 cut(s) 300, 339
PspGI CCWGG 2 cut(s) 300, 339
PspN4I GGNNCC 4 cut(s) 16, 34, 145, 265
PspPI GGNCC 4 cut(s) 32, 58, 136, 304
PsuI RGATCY 1 cut(s) 102
PsyI GACNNNGTC 1 cut(s) 56
SacI GAGCTC 2 cut(s) 120, 276
SatI GCNGC 4 cut(s) 91, 262, 267, 352
Sau3AI GATC 4 cut(s) 102, 184, 289, 393
Sau96I GGNCC 4 cut(s) 32, 58, 136, 304
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 2 cut(s) 302, 341
SduI GDGCHC 4 cut(s) 19, 120, 276, 348
SetI ASST 9 cut(s) 26, 60, 69, 120, 132, 153, 168, 276, 342
SexAI ACCWGGT 1 cut(s) 339
SfaNI GCATC 1 cut(s) 397
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 2 cut(s) 58, 304
SmlI CTYRAG 2 cut(s) 131, 167
SmoI CTYRAG 2 cut(s) 131, 167
Sse9I AATT 1 cut(s) 430
SsiI CCGC 5 cut(s) 90, 261, 267, 307, 352
SspDI GGCGCC 1 cut(s) 263
SstI GAGCTC 2 cut(s) 120, 276
StyD4I CCNGG 2 cut(s) 300, 339
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 153
TaqI TCGA 9 cut(s) 26, 81, 183, 189, 219, 288, 375, 405, 435
TasI AATT 1 cut(s) 430
TauI GCSGC 4 cut(s) 93, 264, 269, 354
TseFI GTSAC 1 cut(s) 46
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 3 cut(s) 27, 97, 391
Tth111I GACNNNGTC 1 cut(s) 56
VpaK11BI GGWCC 2 cut(s) 58, 304
XapI RAATTY 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.