Rh7BG016000

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0007579
Physical Location & Seq
Forward (+)
1023160 .. 1026510
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG016000.1

Sequence Viewer

Length: 498 bp
ATGGCTTCCAACGGAACCCCTACCACCACCGCCGACGCCAAACCCGTCGTGACCGACGAGGTCCGCCGCGTCTTCAAGAGCTTCGACGCCAACGGCGACGGCAAGATCTCAACCTCCGAGCTCGGCAACGTCCTCGAGGCCCTCGGCTCCAACGTCCCCGAGGACGAGCTCCAGCGCGTGATGGTCGACCTCGACACCGACCGCGACGGCTTCATCTGCCTCGACGAGTTCAACGCCTTCTGGGTGGCCGGCGACGGCGGCTCCTCCGAGCTCAAGGAGGCCTTCGATCTGTACGACCAGGACCGCAACGGCCTCATCTCCGTCGACGAGCTCCACCTCGTCCTCAACCGGCTTCAGATGAAGTGCACCGTCGAGGATTGCAACCGCATGATCCAGTCCGTTGACGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGAGCCACAACTCCAGCAACGCCGGCGCCGTCTCTAACGGCGCCTGA

Protein Analysis

165

Amino Acids

17.93

Weight (kDa)

4.24

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 20 - 47 7.8e-07 EF hand domain
EF-hand_7 PF13499 20 - 80 2.8e-14 EF-hand domain pair
EF-hand_6 PF13405 20 - 49 9.1e-08 EF-hand domain
EF-hand_5 PF13202 23 - 44 9.1e-07 EF hand
EF-hand_7 PF13499 89 - 152 4.3e-15 EF-hand domain pair
EF-hand_1 PF00036 90 - 117 1e-06 EF hand domain
EF-hand_6 PF13405 90 - 117 6.3e-07 EF-hand domain
EF-hand_1 PF00036 127 - 153 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 134
AccB1I GGYRCC 2 cut(s) 476, 491
AccI GTMKAC 2 cut(s) 186, 324
AccII CGCG 3 cut(s) 69, 177, 204
AciI CCGC 7 cut(s) 30, 64, 67, 202, 258, 304, 385
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 1 cut(s) 338
AcyI GRCGYC 5 cut(s) 36, 87, 405, 477, 492
AfaI GTAC 1 cut(s) 293
AgsI TTSAA 3 cut(s) 76, 232, 442
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 5 cut(s) 81, 121, 169, 271, 331
AluI AGCT 5 cut(s) 81, 121, 169, 271, 331
Alw21I GWGCWC 5 cut(s) 123, 171, 273, 333, 368
Alw26I GTCTC 1 cut(s) 487
Alw44I GTGCAC 1 cut(s) 364
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 2 cut(s) 134, 158
AoxI GGCC 4 cut(s) 138, 246, 279, 310
ApaLI GTGCAC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 427
AspLEI GCGC 3 cut(s) 177, 479, 494
AspS9I GGNCC 3 cut(s) 61, 139, 301
AvaI CYCGRG 2 cut(s) 134, 158
AvaII GGWCC 2 cut(s) 61, 301
BaeGI GKGCMC 1 cut(s) 368
BanI GGYRCC 2 cut(s) 476, 491
BanII GRGCYC 4 cut(s) 123, 171, 273, 333
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 5 cut(s) 123, 171, 273, 333, 368
BccI CCATC 1 cut(s) 175
BceAI ACGGC 7 cut(s) 109, 115, 223, 271, 325, 427, 464
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 299
BcoDI GTCTC 1 cut(s) 487
BfoI RGCGCY 2 cut(s) 480, 495
BglII AGATCT 1 cut(s) 105
BisI GCNGC 2 cut(s) 67, 259
BlsI GCNGC 2 cut(s) 68, 260
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 2 cut(s) 61, 301
BmeT110I CYCGRG 2 cut(s) 134, 158
BmgT120I GGNCC 3 cut(s) 61, 139, 301
BmiI GGNNCC 5 cut(s) 16, 148, 262, 478, 493
BmrFI CCNGG 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 2 cut(s) 155, 448
BpuEI CTTGAG 1 cut(s) 257
BsaHI GRCGYC 5 cut(s) 36, 87, 405, 477, 492
BsaJI CCNNGG 2 cut(s) 142, 159
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bse118I RCCGGY 3 cut(s) 248, 348, 473
Bse1I ACTGG 1 cut(s) 394
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 142, 159
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 253, 449
BseSI GKGCMC 1 cut(s) 368
Bsh1236I CGCG 3 cut(s) 69, 177, 204
Bsh1285I CGRYCG 1 cut(s) 202
BshFI GGCC 4 cut(s) 140, 248, 281, 312
BshNI GGYRCC 2 cut(s) 476, 491
BsiEI CGRYCG 1 cut(s) 202
BsiHKAI GWGCWC 5 cut(s) 123, 171, 273, 333, 368
BsiHKCI CYCGRG 2 cut(s) 134, 158
BsiSI CCGG 3 cut(s) 249, 349, 474
BslFI GGGAC 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 487
BsmBI CGTCTC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 4 cut(s) 140, 248, 281, 312
BsoBI CYCGRG 2 cut(s) 134, 158
Bsp1286I GDGCHC 5 cut(s) 123, 171, 273, 333, 368
Bsp143I GATC 3 cut(s) 105, 286, 390
BspACI CCGC 7 cut(s) 30, 64, 67, 202, 258, 304, 385
BspANI GGCC 4 cut(s) 140, 248, 281, 312
BspFNI CGCG 3 cut(s) 69, 177, 204
BspLI GGNNCC 5 cut(s) 16, 148, 262, 478, 493
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 2 cut(s) 476, 491
BsrFI RCCGGY 3 cut(s) 248, 348, 473
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 3 cut(s) 248, 348, 473
BssECI CCNNGG 2 cut(s) 142, 159
BssMI GATC 3 cut(s) 105, 286, 390
BssNI GRCGYC 5 cut(s) 36, 87, 405, 477, 492
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 370
BstACI GRCGYC 5 cut(s) 36, 87, 405, 477, 492
BstC8I GCNNGC 2 cut(s) 250, 475
BstFNI CGCG 3 cut(s) 69, 177, 204
BstH2I RGCGCY 2 cut(s) 480, 495
BstHHI GCGC 3 cut(s) 177, 479, 494
BstKTI GATC 3 cut(s) 108, 289, 393
BstMAI GTCTC 1 cut(s) 487
BstMBI GATC 3 cut(s) 105, 286, 390
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 3 cut(s) 216, 258, 474
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 3 cut(s) 69, 177, 204
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 4 cut(s) 140, 248, 281, 312
Cac8I GCNNGC 2 cut(s) 250, 475
CfoI GCGC 3 cut(s) 177, 479, 494
Cfr10I RCCGGY 3 cut(s) 248, 348, 473
Cfr13I GGNCC 3 cut(s) 61, 139, 301
CseI GACGC 4 cut(s) 44, 58, 95, 413
Csp6I GTAC 1 cut(s) 292
CviAII CATG 1 cut(s) 388
CviQI GTAC 1 cut(s) 292
DinI GGCGCC 2 cut(s) 478, 493
DpnI GATC 3 cut(s) 107, 288, 392
DpnII GATC 3 cut(s) 105, 286, 390
EaeI YGGCCR 1 cut(s) 246
EciI GGCGGA 1 cut(s) 53
Ecl136II GAGCTC 4 cut(s) 121, 169, 271, 331
Eco147I AGGCCT 1 cut(s) 281
Eco24I GRGCYC 4 cut(s) 123, 171, 273, 333
Eco47I GGWCC 2 cut(s) 61, 301
Eco53kI GAGCTC 4 cut(s) 121, 169, 271, 331
Eco57I CTGAAG 1 cut(s) 338
Eco88I CYCGRG 2 cut(s) 134, 158
EcoICRI GAGCTC 4 cut(s) 121, 169, 271, 331
EcoO109I RGGNCCY 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 297
EcoT38I GRGCYC 4 cut(s) 123, 171, 273, 333
EgeI GGCGCC 2 cut(s) 478, 493
EheI GGCGCC 2 cut(s) 478, 493
Esp3I CGTCTC 1 cut(s) 487
FaeI CATG 1 cut(s) 391
FaiI YATR 1 cut(s) 389
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 1 cut(s) 140
FatI CATG 1 cut(s) 387
FblI GTMKAC 2 cut(s) 186, 324
Fnu4HI GCNGC 2 cut(s) 67, 259
FriOI GRGCYC 4 cut(s) 123, 171, 273, 333
Fsp4HI GCNGC 2 cut(s) 67, 259
GlaI GCGC 3 cut(s) 176, 478, 493
GluI GCNGC 2 cut(s) 67, 259
GsuI CTGGAG 2 cut(s) 155, 448
HaeII RGCGCY 2 cut(s) 480, 495
HaeIII GGCC 4 cut(s) 140, 248, 281, 312
HapII CCGG 3 cut(s) 249, 349, 474
HgaI GACGC 4 cut(s) 44, 58, 95, 413
HhaI GCGC 3 cut(s) 177, 479, 494
Hin1I GRCGYC 5 cut(s) 36, 87, 405, 477, 492
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 3 cut(s) 175, 477, 492
HinP1I GCGC 3 cut(s) 175, 477, 492
HincII GTYRAC 3 cut(s) 187, 325, 403
HindII GTYRAC 3 cut(s) 187, 325, 403
HpaII CCGG 3 cut(s) 249, 349, 474
Hpy166II GTNNAC 4 cut(s) 187, 325, 366, 403
Hpy188I TCNGA 3 cut(s) 118, 268, 357
Hpy188III TCNNGA 3 cut(s) 49, 76, 442
Hpy8I GTNNAC 4 cut(s) 187, 325, 366, 403
HpyAV CCTTC 2 cut(s) 247, 292
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 2 cut(s) 129, 153
HpyCH4V TGCA 2 cut(s) 366, 381
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 258, 474
HpySE526I ACGT 2 cut(s) 129, 153
Hsp92I GRCGYC 5 cut(s) 36, 87, 405, 477, 492
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 3 cut(s) 175, 477, 492
KasI GGCGCC 2 cut(s) 476, 491
KroI GCCGGC 2 cut(s) 248, 473
KroNI GCCGGC 2 cut(s) 250, 475
Kzo9I GATC 3 cut(s) 105, 286, 390
LmnI GCTCC 4 cut(s) 152, 174, 266, 336
LpnPI CCDG 9 cut(s) 185, 226, 262, 284, 311, 362, 407, 478, 487
MaeII ACGT 2 cut(s) 129, 153
MaeIII GTNAC 1 cut(s) 49
MalI GATC 3 cut(s) 107, 288, 392
MboI GATC 3 cut(s) 105, 286, 390
MboII GAAGA 2 cut(s) 64, 457
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 5 cut(s) 123, 171, 273, 333, 368
MluCI AATT 1 cut(s) 427
Mly113I GGCGCC 2 cut(s) 477, 492
MmeI TCCRAC 2 cut(s) 33, 174
MreI CGCCGGCG 1 cut(s) 473
MroNI GCCGGC 2 cut(s) 248, 473
MspI CCGG 3 cut(s) 249, 349, 474
MspR9I CCNGG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 3 cut(s) 69, 177, 204
MwoI GCNNNNNNNGC 3 cut(s) 216, 258, 474
NaeI GCCGGC 2 cut(s) 250, 475
NarI GGCGCC 2 cut(s) 477, 492
NdeII GATC 3 cut(s) 105, 286, 390
NgoMIV GCCGGC 2 cut(s) 248, 473
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 5 cut(s) 16, 148, 262, 478, 493
NmeAIII GCCGAG 2 cut(s) 102, 123
NmuCI GTSAC 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 134
PceI AGGCCT 1 cut(s) 281
PcsI WCGNNNNNNNCGW 7 cut(s) 42, 54, 93, 150, 291, 411, 477
PdiI GCCGGC 2 cut(s) 250, 475
PflFI GACNNNGTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 68, 260
PluTI GGCGCC 2 cut(s) 480, 495
Psp124BI GAGCTC 4 cut(s) 123, 171, 273, 333
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 5 cut(s) 16, 148, 262, 478, 493
PspPI GGNCC 3 cut(s) 61, 139, 301
PspXI VCTCGAGB 1 cut(s) 134
PsuI RGATCY 1 cut(s) 105
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
SacI GAGCTC 4 cut(s) 123, 171, 273, 333
SalI GTCGAC 2 cut(s) 185, 323
SatI GCNGC 2 cut(s) 67, 259
Sau3AI GATC 3 cut(s) 105, 286, 390
Sau96I GGNCC 3 cut(s) 61, 139, 301
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 5 cut(s) 123, 171, 273, 333, 368
SfoI GGCGCC 2 cut(s) 478, 493
Sfr274I CTCGAG 1 cut(s) 134
SgrAI CRCCGGYG 1 cut(s) 473
SgrDI CGTCGACG 1 cut(s) 323
SinI GGWCC 2 cut(s) 61, 301
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 2 cut(s) 134, 272
SmoI CTYRAG 2 cut(s) 134, 272
Sse9I AATT 1 cut(s) 427
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 7 cut(s) 30, 64, 67, 202, 258, 304, 385
SspDI GGCGCC 2 cut(s) 476, 491
SstI GAGCTC 4 cut(s) 123, 171, 273, 333
StuI AGGCCT 1 cut(s) 281
StyD4I CCNGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 2 cut(s) 132, 156
TaqI TCGA 9 cut(s) 84, 135, 186, 192, 222, 285, 324, 372, 432
TaqII GACCGA 1 cut(s) 68
TasI AATT 1 cut(s) 427
TauI GCSGC 2 cut(s) 69, 261
TseFI GTSAC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 2 cut(s) 202, 374
TspGWI ACGGA 3 cut(s) 27, 310, 388
Tth111I GACNNNGTC 1 cut(s) 59
VneI GTGCAC 1 cut(s) 364
VpaK11BI GGWCC 2 cut(s) 61, 301
XapI RAATTY 1 cut(s) 427
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 2 cut(s) 186, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.