RLG00000005438

calcium-binding protein CML27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007579
Physical Location & Seq
Reverse (-)
67635354 .. 67635854
501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005438

Sequence Viewer

Length: 501 bp
ATGGCTTCCAACGGAACCCCAACCACCGCCGTCGCCAACGCCAAACCCGTCGTGACCGACGAGGTCCGCCGCGTCTTCAACAGCTTCGACGCCAACGGCGACGGCAAGATCTCAACCTCCGAGCTCGGCAACGTCCTCAAGGCCCTCGGCTCCAACGTCCCCGAGGACGAGCTCCAGCGCGTGATGGTCGACCTTGACACCGACCACGACGGCTTCATCTGCATCGACGAGTTCAACGCCTTCTGGGTGGCCGGCGACGGCGGCTCCTCCGAGCTCAAGGAAGCCTTCGATCTCTACGACCAGGACCGCAACGGCCTCATCTCCTGCGACGAGCTCCACCTCGTCCTCAACCGGCTTCAGATGAAGTGCACCGTCGAGGATTGCAACCGCATGATCCAGTCCGTTGACGCCGACGGCGACGGGAATGTGAATTTCGAGGAGTTCAAGAAGATGATGAGCCACAACTCCAGCAACGCCGGCACCGTCTCTAACGGCGCCTGA

Protein Analysis

167

Amino Acids

18.0

Weight (kDa)

4.3

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 21 - 49 4.6e-07 EF hand domain
EF-hand_7 PF13499 21 - 81 4.1e-14 EF-hand domain pair
EF-hand_6 PF13405 21 - 50 5.5e-08 EF-hand domain
EF-hand_5 PF13202 24 - 45 8.7e-07 EF hand
EF-hand_7 PF13499 90 - 153 9.4e-15 EF-hand domain pair
EF-hand_1 PF00036 91 - 118 2.4e-06 EF hand domain
EF-hand_6 PF13405 91 - 118 1.8e-06 EF-hand domain
EF-hand_1 PF00036 128 - 154 1.4e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 479, 494
AccI GTMKAC 1 cut(s) 189
AccII CGCG 2 cut(s) 72, 180
AciI CCGC 6 cut(s) 27, 67, 70, 261, 307, 388
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 3 cut(s) 90, 408, 495
AgsI TTSAA 3 cut(s) 79, 235, 445
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 5 cut(s) 84, 124, 172, 274, 334
AluI AGCT 5 cut(s) 84, 124, 172, 274, 334
Alw21I GWGCWC 5 cut(s) 126, 174, 276, 336, 371
Alw26I GTCTC 1 cut(s) 490
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 1 cut(s) 388
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 3 cut(s) 141, 249, 313
ApaLI GTGCAC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 430
AspLEI GCGC 2 cut(s) 180, 497
AspS9I GGNCC 3 cut(s) 64, 142, 304
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 2 cut(s) 64, 304
BaeGI GKGCMC 1 cut(s) 371
BanI GGYRCC 2 cut(s) 479, 494
BanII GRGCYC 4 cut(s) 126, 174, 276, 336
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 5 cut(s) 126, 174, 276, 336, 371
BccI CCATC 1 cut(s) 178
BceAI ACGGC 7 cut(s) 14, 112, 118, 226, 274, 328, 430
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 490
BfoI RGCGCY 1 cut(s) 498
BglII AGATCT 1 cut(s) 108
BisI GCNGC 2 cut(s) 70, 262
BlsI GCNGC 2 cut(s) 71, 263
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 64, 304
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 64, 142, 304
BmiI GGNNCC 5 cut(s) 16, 151, 265, 481, 496
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 2 cut(s) 158, 451
BpuEI CTTGAG 2 cut(s) 122, 260
BsaHI GRCGYC 3 cut(s) 90, 408, 495
BsaJI CCNNGG 2 cut(s) 145, 162
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bse118I RCCGGY 3 cut(s) 251, 351, 476
Bse1I ACTGG 1 cut(s) 397
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 2 cut(s) 145, 162
BseNI ACTGG 1 cut(s) 397
BseRI GAGGAG 2 cut(s) 256, 452
BseSI GKGCMC 1 cut(s) 371
Bsh1236I CGCG 2 cut(s) 72, 180
BshFI GGCC 3 cut(s) 143, 251, 315
BshNI GGYRCC 2 cut(s) 479, 494
BsiHKAI GWGCWC 5 cut(s) 126, 174, 276, 336, 371
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 3 cut(s) 252, 352, 477
BslFI GGGAC 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 490
BsmBI CGTCTC 1 cut(s) 490
BsmFI GGGAC 1 cut(s) 143
BsnI GGCC 3 cut(s) 143, 251, 315
BsoBI CYCGRG 1 cut(s) 161
Bsp1286I GDGCHC 5 cut(s) 126, 174, 276, 336, 371
Bsp143I GATC 3 cut(s) 108, 289, 393
BspACI CCGC 6 cut(s) 27, 67, 70, 261, 307, 388
BspANI GGCC 3 cut(s) 143, 251, 315
BspFNI CGCG 2 cut(s) 72, 180
BspLI GGNNCC 5 cut(s) 16, 151, 265, 481, 496
BspPI GGATC 1 cut(s) 388
BspT107I GGYRCC 2 cut(s) 479, 494
BsrFI RCCGGY 3 cut(s) 251, 351, 476
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 3 cut(s) 251, 351, 476
BssECI CCNNGG 2 cut(s) 145, 162
BssMI GATC 3 cut(s) 108, 289, 393
BssNI GRCGYC 3 cut(s) 90, 408, 495
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 373, 484
BstACI GRCGYC 3 cut(s) 90, 408, 495
BstC8I GCNNGC 2 cut(s) 253, 478
BstFNI CGCG 2 cut(s) 72, 180
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 180, 497
BstKTI GATC 3 cut(s) 111, 292, 396
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 3 cut(s) 108, 289, 393
BstMWI GCNNNNNNNGC 3 cut(s) 219, 261, 477
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSLI GKGCMC 1 cut(s) 371
BstUI CGCG 2 cut(s) 72, 180
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 108
BstYI RGATCY 1 cut(s) 108
BsuRI GGCC 3 cut(s) 143, 251, 315
Cac8I GCNNGC 2 cut(s) 253, 478
CfoI GCGC 2 cut(s) 180, 497
Cfr10I RCCGGY 3 cut(s) 251, 351, 476
Cfr13I GGNCC 3 cut(s) 64, 142, 304
CseI GACGC 3 cut(s) 61, 98, 416
CviAII CATG 1 cut(s) 391
DinI GGCGCC 1 cut(s) 496
DpnI GATC 3 cut(s) 110, 291, 395
DpnII GATC 3 cut(s) 108, 289, 393
EaeI YGGCCR 1 cut(s) 249
EciI GGCGGA 1 cut(s) 56
Ecl136II GAGCTC 4 cut(s) 124, 172, 274, 334
Eco24I GRGCYC 4 cut(s) 126, 174, 276, 336
Eco47I GGWCC 2 cut(s) 64, 304
Eco53kI GAGCTC 4 cut(s) 124, 172, 274, 334
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 161
EcoICRI GAGCTC 4 cut(s) 124, 172, 274, 334
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 1 cut(s) 300
EcoT38I GRGCYC 4 cut(s) 126, 174, 276, 336
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
Esp3I CGTCTC 1 cut(s) 490
FaeI CATG 1 cut(s) 394
FaiI YATR 1 cut(s) 392
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 390
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 2 cut(s) 70, 262
FriOI GRGCYC 4 cut(s) 126, 174, 276, 336
Fsp4HI GCNGC 2 cut(s) 70, 262
GlaI GCGC 2 cut(s) 179, 496
GluI GCNGC 2 cut(s) 70, 262
GsuI CTGGAG 2 cut(s) 158, 451
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 3 cut(s) 143, 251, 315
HapII CCGG 3 cut(s) 252, 352, 477
HgaI GACGC 3 cut(s) 61, 98, 416
HhaI GCGC 2 cut(s) 180, 497
Hin1I GRCGYC 3 cut(s) 90, 408, 495
Hin1II CATG 1 cut(s) 394
Hin6I GCGC 2 cut(s) 178, 495
HinP1I GCGC 2 cut(s) 178, 495
HincII GTYRAC 2 cut(s) 190, 406
HindII GTYRAC 2 cut(s) 190, 406
HpaII CCGG 3 cut(s) 252, 352, 477
Hpy166II GTNNAC 3 cut(s) 190, 369, 406
Hpy188I TCNGA 3 cut(s) 121, 271, 360
Hpy188III TCNNGA 2 cut(s) 52, 445
Hpy8I GTNNAC 3 cut(s) 190, 369, 406
HpyAV CCTTC 2 cut(s) 250, 295
HpyCH4III ACNGT 2 cut(s) 373, 484
HpyCH4IV ACGT 2 cut(s) 132, 156
HpyCH4V TGCA 3 cut(s) 222, 369, 384
HpyF10VI GCNNNNNNNGC 3 cut(s) 219, 261, 477
HpySE526I ACGT 2 cut(s) 132, 156
Hsp92I GRCGYC 3 cut(s) 90, 408, 495
Hsp92II CATG 1 cut(s) 394
HspAI GCGC 2 cut(s) 178, 495
KasI GGCGCC 1 cut(s) 494
KroI GCCGGC 2 cut(s) 251, 476
KroNI GCCGGC 2 cut(s) 253, 478
Kzo9I GATC 3 cut(s) 108, 289, 393
LmnI GCTCC 4 cut(s) 155, 177, 269, 339
LweI GCATC 1 cut(s) 231
MaeII ACGT 2 cut(s) 132, 156
MaeIII GTNAC 1 cut(s) 52
MalI GATC 3 cut(s) 110, 291, 395
MboI GATC 3 cut(s) 108, 289, 393
MboII GAAGA 2 cut(s) 67, 460
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 5 cut(s) 126, 174, 276, 336, 371
MluCI AATT 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 495
MmeI TCCRAC 2 cut(s) 33, 177
MroNI GCCGGC 2 cut(s) 251, 476
MspI CCGG 3 cut(s) 252, 352, 477
MspR9I CCNGG 1 cut(s) 302
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 2 cut(s) 72, 180
MwoI GCNNNNNNNGC 3 cut(s) 219, 261, 477
NaeI GCCGGC 2 cut(s) 253, 478
NarI GGCGCC 1 cut(s) 495
NdeII GATC 3 cut(s) 108, 289, 393
NgoMIV GCCGGC 2 cut(s) 251, 476
NlaIII CATG 1 cut(s) 394
NlaIV GGNNCC 5 cut(s) 16, 151, 265, 481, 496
NmeAIII GCCGAG 2 cut(s) 105, 126
NmuCI GTSAC 1 cut(s) 52
PcsI WCGNNNNNNNCGW 7 cut(s) 45, 57, 96, 153, 294, 414, 480
PdiI GCCGGC 2 cut(s) 253, 478
PflFI GACNNNGTC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 71, 263
PluTI GGCGCC 1 cut(s) 498
Psp124BI GAGCTC 4 cut(s) 126, 174, 276, 336
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 5 cut(s) 16, 151, 265, 481, 496
PspPI GGNCC 3 cut(s) 64, 142, 304
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 62
SacI GAGCTC 4 cut(s) 126, 174, 276, 336
SalI GTCGAC 1 cut(s) 188
SatI GCNGC 2 cut(s) 70, 262
Sau3AI GATC 3 cut(s) 108, 289, 393
Sau96I GGNCC 3 cut(s) 64, 142, 304
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 5 cut(s) 126, 174, 276, 336, 371
SfaNI GCATC 1 cut(s) 231
SfoI GGCGCC 1 cut(s) 496
SinI GGWCC 2 cut(s) 64, 304
SmlI CTYRAG 2 cut(s) 137, 275
SmoI CTYRAG 2 cut(s) 137, 275
Sse9I AATT 1 cut(s) 430
SsiI CCGC 6 cut(s) 27, 67, 70, 261, 307, 388
SspDI GGCGCC 1 cut(s) 494
SstI GAGCTC 4 cut(s) 126, 174, 276, 336
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 373, 484
TaiI ACGT 2 cut(s) 135, 159
TaqI TCGA 6 cut(s) 87, 189, 225, 288, 375, 435
TaqII GACCGA 1 cut(s) 71
TasI AATT 1 cut(s) 430
TauI GCSGC 2 cut(s) 72, 264
TseFI GTSAC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 52
TspDTI ATGAA 2 cut(s) 205, 377
TspGWI ACGGA 2 cut(s) 27, 391
Tth111I GACNNNGTC 1 cut(s) 62
VneI GTGCAC 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 64, 304
XapI RAATTY 1 cut(s) 430
XmiI GTMKAC 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.