Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002931

Physical Location & Seq

Genomic Coordinates Reverse (-)

6650476 .. 6653195

Gene Structure 2720 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G19250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGGCTTCTAACTATGATAAGCTTACTTCTTCGAGAGTAGCCATCATCGGTGCTGGTGTTAGCGGATTAGCAGCCGCTAAGAACTTAGTTCATCACAACCCGACCGTATTCGAAGCCTCGGATTCAGTCGGAGGTGTTTGGAGGAGCTGCACTTACGAGACGACTAAATTACAATCGGCTCGAGTCGATTACGAGTTCTCCGACTTTCCATGGCCCAATAATAGAGATGACACAACTTTCCCACCTTACCTTGAGATATTAGATTACTTGGAATCTTATGCCAAACATTTTGATCTTCTCAAATTCATGAAGTTTGGCTCTAAGGTCATCGAAGTAAGGTTCATCGGTGATGGCGAAACTCCTCAGATGGTTGACCTTGGTGCTTACGGCAACTTGTTGCCCGGAAAACCTGTATGGGAAGTTGCTGTTCAGATCGGAGATTCTGGAGATATTCAGTGGCATGCATTTGAGTTCGTGGTTGTGTGTACCGGGAAATACGGCGACGTTCCAAGAATACCAGCTTTTCCGGCAAAGAAAGGGCCGGAGATGTTTCAAGGAAAAGTAATGCATTCGATGGATTACTGCAAGTTAGAGAAAGAAGAAGCTTCTACTCTCCTCAGTGGCAAAAAAGTAGCCGTCATCGGCTTCAAGAAATCCGCCATTGATTTGGCTTTAGAGTCTGCTTTAGCTAATCAAGGGGAAGGAGGAAAAGCTTGCACAATGGTGGTGAGAACAACTCATTGGGGGATCCCACATTATTGGGTGTGGGGCTTACCATTCTTCTTGTTCTACTCTTCGAGAGCTTCTCAGTTCCTCCATGATAGGCCTAACCAAAGCTTCCTTAGAACTCTCTTTTGCCTCCTCTTCTCTCTTCTGCGTGCCGTAGTTTCCAAGTTCATCGAATCATATGTTTTATGGAAGCTACCTCTTGAGAAATATGGTCTAAAACCAAACCATTCTTTCGAGGAAGACTATGCTTCTTGTCAAATGGCGATCATACCGGAGAATTTCTTTGAGGAAGCGGATAAAGGGATGATCCGGTTTAAGAAATCATCAAAATGGTGGTTTTATGAAGAAGGGATTGTGTTTGAAGATGGGACGACATTAGAAGCTGATGTTGTGATACTTGCTACTGGTTATGATGGCAAGAAGAAGCTCAAAGCTATTGTTCCTGAACCTTTCCGAACTTGGCTTGAGTTTCCAAGCGGTGTCATGCCTTTATACAGGGGAACAATCCATCCATTGATACCAAACATGGGTTTCGTAGGATACGTCCAAAGCAGCTCGAACTTACACACATCAGAGCTACGTTCAATGTGGCTAAGCCGGCTAGTGGATGAGAAATTCAGATTACCAAGCAAAGAGAAGATGTTGGATCAATTCTTAAAAGAAATGGAAGTGACGAGAAACTCAAGCAGATTCTACAAACGTCATTGCATTTCCACGTTCAGCATCCAACATGCTGATGATATGTGCAACGACATGGGACTCAATCCTTGGCGTAAATCCAACTTCCTCCTTGAAGCTTTTAGTCCTTATGGTTCTCAAGATTACCGACTCGGTCAAGAAGAAAAAGAAGATATGACTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001666 GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004497 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009626 GO:0009627 GO:0009628 GO:0009814 GO:0009870 GO:0009987 GO:0010204 GO:0012501 GO:0016491 GO:0023052 GO:0031347 GO:0031349 GO:0033554 GO:0034050 GO:0036293 GO:0036294 GO:0042221 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070482 GO:0070887 GO:0071453 GO:0071456 GO:0080134 GO:0098542

KEGG Pathways

Metabolic & Signaling

ko00982 ko01120 map00982 map01120

Pfam Domains

Protein Families

FMO-like

Protein Analysis

530

Amino Acids

60.4

Weight (kDa)

6.26

Isoelectric Point (pI)

41.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FMO-like	PF00743	11 - 500	1.3e-41	Flavin-binding monooxygenase-like
NAD_binding_8	PF13450	15 - 69	9.8e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G19250 AT5G45180
fragaria_vesca	FvH4_1g09240 FvH4_2g37000
malus_domestica	MD02G1099600.v1.1 MD08G1046700.v1.1 MD08G1046800.v1.1 MD15G1047200.v1.1 MD15G1222400.v1.1
prunus_persica	Prupe.1G401400_v2.0.a1 Prupe.7G193500_v2.0.a1
pyrus_communis	pycom02g07750 pycom08g03910 pycom08g03920 pycom15g04540 pycom15g04600
rosa_chinensis	RchiOBHm_Chr2g0095771 RchiOBHm_Chr6g0304751
rosa_laevigata	RLG00000010953 RLG00000016591
rosa_multiflora	Rmu_co8416307.1_g000001 Rmu_sc0031036.1_g000001 Rmu_ssc0000139.1_g000009
rosa_roxburghii	Rroxscaffold_2G00146110 Rroxscaffold_7G00163530
rosa_rugosa	Rorug02G0053100 Rorug06G0334200
rosa_samantha	Rh2AG101500 Rh2AG101600 Rh2BG103200 Rh2CG105500 Rh2DG102900 Rh6AG446100 Rh6BG455500 Rh6CG459500 Rh6DG446400
rosa_wichuraiana	Rw2G007710 Rw6G038810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	5 cut(s)	63, 75, 657, 1022, 1206
AclWI	GGATC	4 cut(s)	742, 755, 1030, 1383
AcsI	RAATTY	3 cut(s)	302, 1006, 1343
AfaI	GTAC	1 cut(s)	487
AfiI	CCNNNNNNNGG	2 cut(s)	1256, 1333
AgsI	TTSAA	5 cut(s)	554, 649, 1091, 1314, 1523
AjuI	GAANNNNNNNTTGG	2 cut(s)	724, 756
AleI	CACNNNNGTG	1 cut(s)	722
AloI	GAACNNNNNNTCC	2 cut(s)	1222, 1254
Alw26I	GTCTC	1 cut(s)	152
AlwI	GGATC	4 cut(s)	742, 755, 1030, 1383
Ama87I	CYCGRG	1 cut(s)	180
AoxI	GGCC	3 cut(s)	212, 539, 824
ApeKI	GCWGC	3 cut(s)	71, 147, 1281
ApoI	RAATTY	3 cut(s)	302, 1006, 1343
AspS9I	GGNCC	2 cut(s)	213, 539
AsuC2I	CCSGG	2 cut(s)	402, 490
AsuHPI	GGTGA	2 cut(s)	359, 739
AsuII	TTCGAA	1 cut(s)	111
AvaI	CYCGRG	1 cut(s)	180
BamHI	GGATCC	1 cut(s)	747
BbsI	GAAGAC	1 cut(s)	975
BbvI	GCAGC	3 cut(s)	83, 134, 1293
BccI	CCATC	7 cut(s)	50, 344, 361, 568, 1088, 1136, 1245
BceAI	ACGGC	4 cut(s)	403, 514, 620, 866
BciVI	GTATCC	1 cut(s)	1262
BcnI	CCSGG	2 cut(s)	402, 490
BcoDI	GTCTC	1 cut(s)	152
BfaI	CTAG	1 cut(s)	1331
BfuI	GTATCC	1 cut(s)	1262
BisI	GCNGC	4 cut(s)	72, 75, 148, 1282
BlpI	GCTNAGC	1 cut(s)	1322
BlsI	GCNGC	4 cut(s)	73, 76, 149, 1283
Bme1390I	CCNGG	2 cut(s)	402, 490
BmeT110I	CYCGRG	1 cut(s)	180
BmgT120I	GGNCC	2 cut(s)	213, 539
BmiI	GGNNCC	1 cut(s)	749
BmrFI	CCNGG	2 cut(s)	402, 490
BmsI	GCATC	1 cut(s)	1461
BpiI	GAAGAC	1 cut(s)	975
BplI	GAGNNNNNCTC	4 cut(s)	721, 753, 790, 822
BpmI	CTGGAG	1 cut(s)	465
Bpu1102I	GCTNAGC	1 cut(s)	1322
Bpu14I	TTCGAA	1 cut(s)	111
BpuEI	CTTGAG	5 cut(s)	272, 950, 1214, 1396, 1530
BpuMI	CCSGG	2 cut(s)	402, 490
BsaJI	CCNNGG	4 cut(s)	117, 209, 376, 1496
BsaWI	WCCGGW	2 cut(s)	1000, 1038
BsaXI	ACNNNNNCTCC	2 cut(s)	182, 212
Bsc4I	CCNNNNNNNGG	2 cut(s)	1256, 1333
Bse118I	RCCGGY	1 cut(s)	1326
Bse1I	ACTGG	1 cut(s)	1138
Bse3DI	GCAATG	1 cut(s)	1432
BseDI	CCNNGG	4 cut(s)	117, 209, 376, 1496
BseGI	GGATG	4 cut(s)	1038, 1237, 1342, 1452
BseLI	CCNNNNNNNGG	2 cut(s)	1256, 1333
BseMI	GCAATG	1 cut(s)	1432
BseMII	CTCAG	3 cut(s)	377, 631, 821
BseNI	ACTGG	1 cut(s)	1138
BseRI	GAGGAG	4 cut(s)	157, 351, 605, 851
BseXI	GCAGC	3 cut(s)	83, 134, 1293
BsgI	GTGCAG	1 cut(s)	133
Bsh1285I	CGRYCG	1 cut(s)	105
BshFI	GGCC	3 cut(s)	214, 541, 826
BsiEI	CGRYCG	1 cut(s)	105
BsiHKCI	CYCGRG	1 cut(s)	180
BsiSI	CCGG	7 cut(s)	402, 489, 527, 542, 1001, 1039, 1327
BslFI	GGGAC	2 cut(s)	1111, 1500
BslI	CCNNNNNNNGG	2 cut(s)	1256, 1333
BsmAI	GTCTC	1 cut(s)	152
BsmBI	CGTCTC	1 cut(s)	152
BsmFI	GGGAC	2 cut(s)	1111, 1500
BsmI	GAATGC	1 cut(s)	568
BsnI	GGCC	3 cut(s)	214, 541, 826
BsoBI	CYCGRG	1 cut(s)	180
Bsp119I	TTCGAA	1 cut(s)	111
Bsp143I	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
Bsp1720I	GCTNAGC	1 cut(s)	1322
Bsp19I	CCATGG	1 cut(s)	209
BspACI	CCGC	5 cut(s)	63, 75, 657, 1022, 1206
BspANI	GGCC	3 cut(s)	214, 541, 826
BspCNI	CTCAG	3 cut(s)	376, 630, 820
BspHI	TCATGA	1 cut(s)	306
BspLI	GGNNCC	1 cut(s)	749
BspPI	GGATC	4 cut(s)	742, 755, 1030, 1383
BspT104I	TTCGAA	1 cut(s)	111
BsrDI	GCAATG	1 cut(s)	1432
BsrFI	RCCGGY	1 cut(s)	1326
BsrI	ACTGG	1 cut(s)	1138
BssAI	RCCGGY	1 cut(s)	1326
BssECI	CCNNGG	4 cut(s)	117, 209, 376, 1496
BssMI	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
BssT1I	CCWWGG	3 cut(s)	209, 376, 1496
Bst4CI	ACNGT	1 cut(s)	106
Bst6I	CTCTTC	3 cut(s)	799, 869, 876
BstBI	TTCGAA	1 cut(s)	111
BstC8I	GCNNGC	4 cut(s)	462, 715, 879, 1328
BstDEI	CTNAG	8 cut(s)	78, 85, 321, 363, 617, 807, 842, 1322
BstDSI	CCRYGG	1 cut(s)	209
BstF5I	GGATG	4 cut(s)	1038, 1237, 1342, 1452
BstKTI	GATC	6 cut(s)	295, 435, 750, 996, 1038, 1378
BstMAI	GTCTC	1 cut(s)	152
BstMBI	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
BstMCI	CGRYCG	1 cut(s)	105
BstMWI	GCNNNNNNNGC	2 cut(s)	527, 1327
BstNSI	RCATGY	2 cut(s)	464, 1463
BstSCI	CCNGG	2 cut(s)	400, 488
BstV1I	GCAGC	3 cut(s)	83, 134, 1293
BstV2I	GAAGAC	1 cut(s)	975
BstX2I	RGATCY	1 cut(s)	747
BstXI	CCANNNNNNTGG	2 cut(s)	667, 759
BstYI	RGATCY	1 cut(s)	747
BsuI	GTATCC	1 cut(s)	1262
BsuRI	GGCC	3 cut(s)	214, 541, 826
BtgI	CCRYGG	1 cut(s)	209
BtsCI	GGATG	4 cut(s)	1038, 1237, 1342, 1452
BtsIMutI	CAGTG	2 cut(s)	461, 625
Cac8I	GCNNGC	4 cut(s)	462, 715, 879, 1328
CciI	TCATGA	1 cut(s)	306
Cfr10I	RCCGGY	1 cut(s)	1326
Cfr13I	GGNCC	2 cut(s)	213, 539
Csp6I	GTAC	1 cut(s)	486
CviAII	CATG	8 cut(s)	210, 307, 461, 818, 1213, 1255, 1460, 1483
CviQI	GTAC	1 cut(s)	486
DdeI	CTNAG	8 cut(s)	78, 85, 321, 363, 617, 807, 842, 1322
DpnI	GATC	6 cut(s)	294, 434, 749, 995, 1037, 1377
DpnII	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
Eam1104I	CTCTTC	3 cut(s)	799, 869, 876
EarI	CTCTTC	3 cut(s)	799, 869, 876
EciI	GGCGGA	1 cut(s)	646
Eco130I	CCWWGG	3 cut(s)	209, 376, 1496
Eco147I	AGGCCT	1 cut(s)	826
Eco88I	CYCGRG	1 cut(s)	180
EcoT14I	CCWWGG	3 cut(s)	209, 376, 1496
EcoT22I	ATGCAT	2 cut(s)	466, 570
ErhI	CCWWGG	3 cut(s)	209, 376, 1496
Esp3I	CGTCTC	1 cut(s)	152
FaeI	CATG	8 cut(s)	213, 310, 464, 821, 1216, 1258, 1463, 1486
FaqI	GGGAC	2 cut(s)	1111, 1500
FatI	CATG	8 cut(s)	209, 306, 460, 817, 1212, 1254, 1459, 1482
FauNDI	CATATG	1 cut(s)	907
Fnu4HI	GCNGC	4 cut(s)	72, 75, 148, 1282
FokI	GGATG	4 cut(s)	1045, 1224, 1349, 1439
Fsp4HI	GCNGC	4 cut(s)	72, 75, 148, 1282
FspBI	CTAG	1 cut(s)	1331
GluI	GCNGC	4 cut(s)	72, 75, 148, 1282
GsuI	CTGGAG	1 cut(s)	465
HaeIII	GGCC	3 cut(s)	214, 541, 826
HapII	CCGG	7 cut(s)	402, 489, 527, 542, 1001, 1039, 1327
Hin1II	CATG	8 cut(s)	213, 310, 464, 821, 1216, 1258, 1463, 1486
HincII	GTYRAC	1 cut(s)	373
HindII	GTYRAC	1 cut(s)	373
HindIII	AAGCTT	5 cut(s)	20, 603, 711, 835, 1524
HinfI	GANTC	9 cut(s)	122, 183, 272, 440, 677, 902, 1419, 1488, 1557
HpaII	CCGG	7 cut(s)	402, 489, 527, 542, 1001, 1039, 1327
HphI	GGTGA	2 cut(s)	359, 739
Hpy166II	GTNNAC	2 cut(s)	373, 486
Hpy188I	TCNGA	9 cut(s)	121, 131, 202, 366, 432, 437, 1184, 1303, 1349
Hpy188III	TCNNGA	9 cut(s)	33, 307, 444, 649, 798, 929, 1172, 1547, 1565
Hpy8I	GTNNAC	2 cut(s)	373, 486
Hpy99I	CGWCG	1 cut(s)	506
HpyAV	CCTTC	2 cut(s)	695, 1070
HpyCH4III	ACNGT	1 cut(s)	106
HpyCH4IV	ACGT	5 cut(s)	504, 1272, 1309, 1429, 1445
HpyCH4V	TGCA	7 cut(s)	150, 464, 568, 585, 717, 1437, 1476
HpyF10VI	GCNNNNNNNGC	2 cut(s)	527, 1327
HpyF3I	CTNAG	8 cut(s)	78, 85, 321, 363, 617, 807, 842, 1322
HpySE526I	ACGT	5 cut(s)	504, 1272, 1309, 1429, 1445
Hsp92II	CATG	8 cut(s)	213, 310, 464, 821, 1216, 1258, 1463, 1486
KroI	GCCGGC	1 cut(s)	1326
KroNI	GCCGGC	1 cut(s)	1328
Kzo9I	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
LmnI	GCTCC	1 cut(s)	144
Lsp1109I	GCAGC	3 cut(s)	83, 134, 1293
LweI	GCATC	1 cut(s)	1461
MaeI	CTAG	1 cut(s)	1331
MaeII	ACGT	5 cut(s)	504, 1272, 1309, 1429, 1445
MaeIII	GTNAC	1 cut(s)	1399
MalI	GATC	6 cut(s)	294, 434, 749, 995, 1037, 1377
MboI	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
MflI	RGATCY	1 cut(s)	747
MluCI	AATT	5 cut(s)	167, 302, 1006, 1343, 1379
MlyI	GAGTC	4 cut(s)	192, 686, 1482, 1551
MmeI	TCCRAC	5 cut(s)	109, 225, 1353, 1480, 1533
Mph1103I	ATGCAT	2 cut(s)	466, 570
MroNI	GCCGGC	1 cut(s)	1326
MseI	TTAA	3 cut(s)	1044, 1385, 1591
MslI	CAYNNNNRTG	3 cut(s)	722, 1057, 1464
MspI	CCGG	7 cut(s)	402, 489, 527, 542, 1001, 1039, 1327
MspR9I	CCNGG	2 cut(s)	402, 490
Mva1269I	GAATGC	1 cut(s)	568
MwoI	GCNNNNNNNGC	2 cut(s)	527, 1327
NaeI	GCCGGC	1 cut(s)	1328
NciI	CCSGG	2 cut(s)	402, 490
NcoI	CCATGG	1 cut(s)	209
NdeI	CATATG	1 cut(s)	907
NdeII	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
NgoMIV	GCCGGC	1 cut(s)	1326
NlaIII	CATG	8 cut(s)	213, 310, 464, 821, 1216, 1258, 1463, 1486
NlaIV	GGNNCC	1 cut(s)	749
NmuCI	GTSAC	1 cut(s)	1399
NsiI	ATGCAT	2 cut(s)	466, 570
NspI	RCATGY	2 cut(s)	464, 1463
NspV	TTCGAA	1 cut(s)	111
OliI	CACNNNNGTG	1 cut(s)	722
PaeI	GCATGC	1 cut(s)	464
PaeR7I	CTCGAG	1 cut(s)	180
PagI	TCATGA	1 cut(s)	306
PceI	AGGCCT	1 cut(s)	826
PcsI	WCGNNNNNNNCGW	3 cut(s)	198, 351, 1269
PctI	GAATGC	1 cut(s)	568
PdiI	GCCGGC	1 cut(s)	1328
PfeI	GAWTC	5 cut(s)	122, 272, 440, 902, 1419
PflFI	GACNNNGTC	1 cut(s)	1560
PkrI	GCNGC	4 cut(s)	73, 76, 149, 1283
PleI	GAGTC	4 cut(s)	191, 685, 1482, 1551
PpsI	GAGTC	4 cut(s)	191, 685, 1482, 1551
PspN4I	GGNNCC	1 cut(s)	749
PspPI	GGNCC	2 cut(s)	213, 539
PspXI	VCTCGAGB	1 cut(s)	180
PsuI	RGATCY	1 cut(s)	747
PsyI	GACNNNGTC	1 cut(s)	1560
RsaI	GTAC	1 cut(s)	487
RsaNI	GTAC	1 cut(s)	486
RseI	CAYNNNNRTG	3 cut(s)	722, 1057, 1464
SaqAI	TTAA	3 cut(s)	1044, 1385, 1591
SatI	GCNGC	4 cut(s)	72, 75, 148, 1282
Sau3AI	GATC	6 cut(s)	292, 432, 747, 993, 1035, 1375
Sau96I	GGNCC	2 cut(s)	213, 539
SchI	GAGTC	4 cut(s)	192, 686, 1482, 1551
ScrFI	CCNGG	2 cut(s)	402, 490
SfaNI	GCATC	1 cut(s)	1461
Sfr274I	CTCGAG	1 cut(s)	180
SfuI	TTCGAA	1 cut(s)	111
SlaI	CTCGAG	1 cut(s)	180
SmiMI	CAYNNNNRTG	3 cut(s)	722, 1057, 1464
SmlI	CTYRAG	6 cut(s)	180, 251, 929, 1193, 1411, 1545
SmoI	CTYRAG	6 cut(s)	180, 251, 929, 1193, 1411, 1545
SphI	GCATGC	1 cut(s)	464
Sse9I	AATT	5 cut(s)	167, 302, 1006, 1343, 1379
SseBI	AGGCCT	1 cut(s)	826
SsiI	CCGC	5 cut(s)	63, 75, 657, 1022, 1206
SspMI	CTAG	1 cut(s)	1331
StuI	AGGCCT	1 cut(s)	826
StyD4I	CCNGG	2 cut(s)	400, 488
StyI	CCWWGG	3 cut(s)	209, 376, 1496
TaaI	ACNGT	1 cut(s)	106
TaiI	ACGT	5 cut(s)	507, 1275, 1312, 1432, 1448
TaqII	GACCGA	1 cut(s)	1550
TasI	AATT	5 cut(s)	167, 302, 1006, 1343, 1379
TauI	GCSGC	1 cut(s)	77
TfiI	GAWTC	5 cut(s)	122, 272, 440, 902, 1419
Tru1I	TTAA	3 cut(s)	1044, 1385, 1591
Tru9I	TTAA	3 cut(s)	1044, 1385, 1591
TscAI	CASTG	2 cut(s)	461, 625
TseFI	GTSAC	1 cut(s)	1399
TseI	GCWGC	3 cut(s)	71, 147, 1281
Tsp45I	GTSAC	1 cut(s)	1399
TspDTI	ATGAA	6 cut(s)	80, 295, 323, 331, 886, 1086
TspRI	CASTG	2 cut(s)	461, 625
Tth111I	GACNNNGTC	1 cut(s)	1560
XapI	RAATTY	3 cut(s)	302, 1006, 1343
XceI	RCATGY	2 cut(s)	464, 1463
XhoI	CTCGAG	1 cut(s)	180
XspI	CTAG	1 cut(s)	1331
Zsp2I	ATGCAT	2 cut(s)	466, 570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.