Rh2AG101500

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002931
Physical Location & Seq
Forward (+)
8654503 .. 8656963
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG101500.1

Sequence Viewer

Length: 1203 bp
ATGGCTCCGGTTGTCTCAAAAGTTGGGATTATTGGTGCCGGAGTGAGCGGCATAGCGGCTGCGAAACACCTAACCCATCACAACCCTATTGTCCTCGAGGCCTCTGACTCCATTGGAGGAGTCTGGAGGCATTGCTCGTACAACTCCACAAAGCTTCAGTCTCATCGACGCGATTACGAGTTCTCTGACTTTCCTTGGAATGATAGGGACAATCCAGAATACCCTTCTCACCTTGAGATATTGGATTACTTGAACTCTTATGCTGAGCACTTTGACGTGCTCAAGTTTGTGAGGTTCAACTCCAAGGTCGTCGAGGTTCGCTTTGTTGGTGACTGGGAAACCATTGATGCTGGTTTTAAACCACCGGCGGGTCAGCCTGCTTGGGAAGTTGCTGTGCAGACCAGTGGTGAAGATACCATTCAGTGGTACGCCTTCGAGTTCCTTGTGGTCTGTATTGGGAAATATGGTGACATACCCAGAATTCCAGATTTTCCACACAACAAAGGCCCTCAAGTGTTTCAAGGTCAGGTTCTGCATTCTGTTGATTACTGCAAACTAGACAAGGAAGCTGCTTCTGACTTACTCAAGGATAAGAAGGTCGTCGTGGTTGGATACAAAAAATCAGCCCTTGATTTAGCTGTGGAGTGTGCTGACGCAAATCAAGGACCAAAAGGGCAACCATGCACAATGGTGGTGAGGACTTTACACTGGACAGTTCCCCATTACTGGGTTTGGGGTTTGCCATTTTTCTTGTTCTATTCAACAAGGGCTTCACAGTTTCTCCATCCAAGACCTAATCAGAGTTTCCTCAGGACCCTCACTTGCCTTCTAGCTTTATCTCCTTTGAGGCATGCAATTTCAAAGTTCATCGAATCATACATTCTGTGGAAGCTTCCTTTGGAGAAGTATGGACTAAAACCAGATCACTCTTTCGAGGAAGACTACGCTTCCTGCCAGATGGCTATCACACCTGAGAACTTGTTCTCCGAGGCTGATAAGGGAAGGATCGTGTTCAAGAGATCACAAGGAACCTGGTGGTTTAATAAAGACGGAATTGAGTTTGACGACGGCAGTAAGGTCGACGCTGATGTTGTGGTCCTTGCAACTGGCTATGATGGTAAGAAGAAGCTCAAAGCCATCTTACCAGATCCCTTCAGAAGCTTGTTGGAATACCCATCTGGTATTATGCCCTTATACAGGTAA

Protein Analysis

400

Amino Acids

45.37

Weight (kDa)

6.4

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 7 - 371 2.3e-31 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 423
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 1 cut(s) 1080
AccII CGCG 1 cut(s) 171
AciI CCGC 3 cut(s) 48, 56, 368
AclWI GGATC 2 cut(s) 1013, 1142
AcsI RAATTY 1 cut(s) 480
AcuI CTGAAG 2 cut(s) 140, 1138
AfaI GTAC 2 cut(s) 140, 428
AfiI CCNNNNNNNGG 5 cut(s) 368, 423, 726, 727, 1197
AgsI TTSAA 6 cut(s) 253, 298, 521, 762, 861, 1015
AjiI CACGTC 1 cut(s) 277
AjnI CCWGG 1 cut(s) 1031
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AleI CACNNNNGTG 1 cut(s) 689
AloI GAACNNNNNNTCC 2 cut(s) 968, 1000
AluBI AGCT 7 cut(s) 154, 569, 638, 833, 892, 1129, 1161
AluI AGCT 7 cut(s) 154, 569, 638, 833, 892, 1129, 1161
Alw21I GWGCWC 2 cut(s) 270, 282
Alw26I GTCTC 2 cut(s) 19, 165
AlwI GGATC 2 cut(s) 1013, 1142
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 2 cut(s) 99, 505
ApeKI GCWGC 2 cut(s) 59, 569
ApoI RAATTY 1 cut(s) 480
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
Asp700I GAANNNNTTC 1 cut(s) 980
AspS9I GGNCC 4 cut(s) 506, 665, 813, 1096
AsuHPI GGTGA 5 cut(s) 221, 341, 419, 479, 706
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 3 cut(s) 665, 813, 1096
AxyI CCTNAGG 1 cut(s) 809
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 945
Bbv12I GWGCWC 2 cut(s) 270, 282
BbvI GCAGC 2 cut(s) 46, 556
BccI CCATC 6 cut(s) 84, 792, 952, 1109, 1145, 1183
BceAI ACGGC 1 cut(s) 1084
BcgI CGANNNNNNTGC 2 cut(s) 1060, 1094
BciT130I CCWGG 1 cut(s) 1033
BciVI GTATCC 1 cut(s) 605
BcoDI GTCTC 2 cut(s) 19, 165
BfaI CTAG 2 cut(s) 557, 830
BfuI GTATCC 1 cut(s) 605
BisI GCNGC 4 cut(s) 49, 57, 60, 570
BlpI GCTNAGC 1 cut(s) 264
BlsI GCNGC 4 cut(s) 50, 58, 61, 571
Bme1390I CCNGG 1 cut(s) 1033
Bme18I GGWCC 3 cut(s) 665, 813, 1096
BmeT110I CYCGRG 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 277
BmgT120I GGNCC 4 cut(s) 506, 665, 813, 1096
BmiI GGNNCC 4 cut(s) 6, 37, 815, 1030
BmrFI CCNGG 1 cut(s) 1033
BmrI ACTGGG 2 cut(s) 343, 736
BmsI GCATC 1 cut(s) 337
BmuI ACTGGG 2 cut(s) 343, 736
BpiI GAAGAC 1 cut(s) 945
BpmI CTGGAG 1 cut(s) 145
Bpu1102I GCTNAGC 1 cut(s) 264
BpuEI CTTGAG 4 cut(s) 254, 266, 495, 569
BsaBI GATNNNNATC 1 cut(s) 962
BsaJI CCNNGG 3 cut(s) 194, 303, 987
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 4 cut(s) 765, 795, 968, 998
Bsc4I CCNNNNNNNGG 5 cut(s) 368, 423, 726, 727, 1197
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 5 cut(s) 338, 402, 713, 731, 1111
Bse21I CCTNAGG 1 cut(s) 809
Bse3DI GCAATG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 962
BseBI CCWGG 1 cut(s) 1033
BseDI CCNNGG 3 cut(s) 194, 303, 987
BseGI GGATG 1 cut(s) 784
BseJI GATNNNNATC 1 cut(s) 962
BseLI CCNNNNNNNGG 5 cut(s) 368, 423, 726, 727, 1197
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 3 cut(s) 255, 823, 963
BseNI ACTGG 5 cut(s) 338, 402, 713, 731, 1111
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 2 cut(s) 46, 556
BsgI GTGCAG 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 101, 507
BshNI GGYRCC 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 270, 282
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 3 cut(s) 8, 39, 365
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 5 cut(s) 368, 423, 726, 727, 1197
BsmAI GTCTC 2 cut(s) 19, 165
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 2 cut(s) 101, 507
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 270, 282
Bsp143I GATC 4 cut(s) 922, 1005, 1019, 1147
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 3 cut(s) 48, 56, 368
BspANI GGCC 2 cut(s) 101, 507
BspCNI CTCAG 3 cut(s) 256, 822, 964
BspFNI CGCG 1 cut(s) 171
BspLI GGNNCC 4 cut(s) 6, 37, 815, 1030
BspPI GGATC 2 cut(s) 1013, 1142
BspT107I GGYRCC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 364
BsrI ACTGG 5 cut(s) 338, 402, 713, 731, 1111
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 3 cut(s) 194, 303, 987
BssMI GATC 4 cut(s) 922, 1005, 1019, 1147
BssT1I CCWWGG 2 cut(s) 194, 303
Bst2UI CCWGG 1 cut(s) 1033
Bst4CI ACNGT 2 cut(s) 715, 777
BstC8I GCNNGC 2 cut(s) 378, 852
BstDEI CTNAG 3 cut(s) 264, 809, 972
BstENI CCTNNNNNAGG 1 cut(s) 1195
BstF5I GGATG 1 cut(s) 784
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 4 cut(s) 925, 1008, 1022, 1150
BstMAI GTCTC 2 cut(s) 19, 165
BstMBI GATC 4 cut(s) 922, 1005, 1019, 1147
BstNI CCWGG 1 cut(s) 1033
BstNSI RCATGY 1 cut(s) 854
BstSCI CCNGG 1 cut(s) 1031
BstUI CGCG 1 cut(s) 171
BstV1I GCAGC 2 cut(s) 46, 556
BstV2I GAAGAC 1 cut(s) 945
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
Bsu36I CCTNAGG 1 cut(s) 809
BsuI GTATCC 1 cut(s) 605
BsuRI GGCC 2 cut(s) 101, 507
BtrI CACGTC 1 cut(s) 277
BtsCI GGATG 1 cut(s) 784
BtsIMutI CAGTG 3 cut(s) 409, 428, 706
Cac8I GCNNGC 2 cut(s) 378, 852
CaiI CAGNNNCTG 1 cut(s) 532
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 4 cut(s) 506, 665, 813, 1096
CseI GACGC 3 cut(s) 177, 662, 1091
CsiI ACCWGGT 1 cut(s) 1031
Csp6I GTAC 2 cut(s) 139, 427
CviAII CATG 2 cut(s) 681, 851
CviQI GTAC 2 cut(s) 139, 427
DdeI CTNAG 3 cut(s) 264, 809, 972
DpnI GATC 4 cut(s) 924, 1007, 1021, 1149
DpnII GATC 4 cut(s) 922, 1005, 1019, 1147
DraI TTTAAA 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 194, 303
Eco147I AGGCCT 1 cut(s) 101
Eco47I GGWCC 3 cut(s) 665, 813, 1096
Eco57I CTGAAG 2 cut(s) 140, 1138
Eco81I CCTNAGG 1 cut(s) 809
Eco88I CYCGRG 1 cut(s) 95
EcoNI CCTNNNNNAGG 1 cut(s) 1195
EcoO109I RGGNCCY 2 cut(s) 506, 813
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 1031
EcoT14I CCWWGG 2 cut(s) 194, 303
ErhI CCWWGG 2 cut(s) 194, 303
FaeI CATG 2 cut(s) 684, 854
FaqI GGGAC 1 cut(s) 221
FatI CATG 2 cut(s) 680, 850
FauI CCCGC 1 cut(s) 361
FblI GTMKAC 1 cut(s) 1080
Fnu4HI GCNGC 4 cut(s) 49, 57, 60, 570
FokI GGATG 1 cut(s) 771
Fsp4HI GCNGC 4 cut(s) 49, 57, 60, 570
FspBI CTAG 2 cut(s) 557, 830
GluI GCNGC 4 cut(s) 49, 57, 60, 570
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 2 cut(s) 101, 507
HapII CCGG 3 cut(s) 8, 39, 365
HgaI GACGC 3 cut(s) 177, 662, 1091
Hin1II CATG 2 cut(s) 684, 854
HincII GTYRAC 1 cut(s) 1081
HindII GTYRAC 1 cut(s) 1081
HindIII AAGCTT 3 cut(s) 152, 890, 1159
HinfI GANTC 3 cut(s) 107, 120, 872
HpaII CCGG 3 cut(s) 8, 39, 365
HphI GGTGA 5 cut(s) 221, 341, 419, 479, 706
Hpy166II GTNNAC 1 cut(s) 1081
Hpy188I TCNGA 6 cut(s) 106, 187, 577, 801, 988, 1157
Hpy188III TCNNGA 5 cut(s) 124, 215, 485, 811, 1015
Hpy8I GTNNAC 1 cut(s) 1081
Hpy99I CGWCG 5 cut(s) 171, 314, 605, 1070, 1085
HpyAV CCTTC 6 cut(s) 234, 442, 589, 836, 996, 1162
HpyCH4III ACNGT 2 cut(s) 715, 777
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 6 cut(s) 397, 535, 552, 684, 854, 1103
HpyF3I CTNAG 3 cut(s) 264, 809, 972
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 2 cut(s) 684, 854
Kzo9I GATC 4 cut(s) 922, 1005, 1019, 1147
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 2 cut(s) 46, 556
LweI GCATC 1 cut(s) 337
MabI ACCWGGT 1 cut(s) 1031
MaeI CTAG 2 cut(s) 557, 830
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 329, 467
MalI GATC 4 cut(s) 924, 1007, 1021, 1149
MbiI CCGCTC 1 cut(s) 48
MboI GATC 4 cut(s) 922, 1005, 1019, 1147
MboII GAAGA 3 cut(s) 422, 950, 1135
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 2 cut(s) 270, 282
MluCI AATT 3 cut(s) 480, 855, 1053
MlyI GAGTC 2 cut(s) 101, 129
MmeI TCCRAC 2 cut(s) 589, 1146
MroXI GAANNNNTTC 1 cut(s) 980
MseI TTAA 2 cut(s) 357, 1041
MslI CAYNNNNRTG 1 cut(s) 689
MspI CCGG 3 cut(s) 8, 39, 365
MspR9I CCNGG 1 cut(s) 1033
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 1 cut(s) 1033
MvnI CGCG 1 cut(s) 171
NdeII GATC 4 cut(s) 922, 1005, 1019, 1147
NlaIII CATG 2 cut(s) 684, 854
NlaIV GGNNCC 4 cut(s) 6, 37, 815, 1030
NmuCI GTSAC 2 cut(s) 329, 467
NspI RCATGY 1 cut(s) 854
OliI CACNNNNGTG 1 cut(s) 689
PaeI GCATGC 1 cut(s) 854
PaeR7I CTCGAG 1 cut(s) 95
PceI AGGCCT 1 cut(s) 101
PctI GAATGC 1 cut(s) 535
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 1 cut(s) 872
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 4 cut(s) 50, 58, 61, 571
PleI GAGTC 2 cut(s) 101, 128
PpsI GAGTC 2 cut(s) 101, 128
PpuMI RGGWCCY 1 cut(s) 813
Psp5II RGGWCCY 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 1031
PspN4I GGNNCC 4 cut(s) 6, 37, 815, 1030
PspPI GGNCC 4 cut(s) 506, 665, 813, 1096
PspPPI RGGWCCY 1 cut(s) 813
PspXI VCTCGAGB 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 1 cut(s) 1147
RsaI GTAC 2 cut(s) 140, 428
RsaNI GTAC 2 cut(s) 139, 427
RseI CAYNNNNRTG 1 cut(s) 689
SalI GTCGAC 1 cut(s) 1079
SaqAI TTAA 2 cut(s) 357, 1041
SatI GCNGC 4 cut(s) 49, 57, 60, 570
Sau3AI GATC 4 cut(s) 922, 1005, 1019, 1147
Sau96I GGNCC 4 cut(s) 506, 665, 813, 1096
SchI GAGTC 2 cut(s) 101, 129
ScrFI CCNGG 1 cut(s) 1033
SduI GDGCHC 2 cut(s) 270, 282
SexAI ACCWGGT 1 cut(s) 1031
SfaNI GCATC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 95
SgrAI CRCCGGYG 1 cut(s) 364
SinI GGWCC 3 cut(s) 665, 813, 1096
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 5 cut(s) 95, 233, 281, 510, 584
SmoI CTYRAG 5 cut(s) 95, 233, 281, 510, 584
SphI GCATGC 1 cut(s) 854
Sse9I AATT 3 cut(s) 480, 855, 1053
SseBI AGGCCT 1 cut(s) 101
SsiI CCGC 3 cut(s) 48, 56, 368
SspMI CTAG 2 cut(s) 557, 830
StuI AGGCCT 1 cut(s) 101
StyD4I CCNGG 1 cut(s) 1031
StyI CCWWGG 2 cut(s) 194, 303
TaaI ACNGT 2 cut(s) 715, 777
TaiI ACGT 1 cut(s) 279
TaqI TCGA 7 cut(s) 96, 166, 312, 435, 870, 933, 1080
TasI AATT 3 cut(s) 480, 855, 1053
TauI GCSGC 2 cut(s) 51, 59
TfiI GAWTC 1 cut(s) 872
Tru1I TTAA 2 cut(s) 357, 1041
Tru9I TTAA 2 cut(s) 357, 1041
TscAI CASTG 3 cut(s) 409, 428, 713
TseFI GTSAC 2 cut(s) 329, 467
TseI GCWGC 2 cut(s) 59, 569
Tsp45I GTSAC 2 cut(s) 329, 467
TspDTI ATGAA 1 cut(s) 856
TspGWI ACGGA 1 cut(s) 1065
TspRI CASTG 3 cut(s) 409, 428, 713
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 3 cut(s) 665, 813, 1096
XagI CCTNNNNNAGG 1 cut(s) 1195
XapI RAATTY 1 cut(s) 480
XceI RCATGY 1 cut(s) 854
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 1080
XmnI GAANNNNTTC 1 cut(s) 980
XspI CTAG 2 cut(s) 557, 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.