AT1G23170

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002090
Physical Location & Seq
Forward (+)
8212556 .. 8215807
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23170.1

Sequence Viewer

Length: 1710 bp
ATGGATCCAATTGAATCAGTGGAGTACAATGGCTTTGAAACTACCAATGGTAACTCTCACAACGACGACCATGGTTGGAAGAAAGTAGTGTACCCAAAACGTAACCGGAAGCAAAAACCGGCGGATCAAGCGGCGGCGACGAAGAATGGTGTCACCGGGAATCTAATTCCCAATGGTACTTTATCCAATGGAGGAGGCAACGTCTTCCGTTCTCTTGAGGAGCAAGCTGAAGGTCGTCATCTACAGATCCTCGCCGCGAAGAAAGCTTCCGATACGGCGGATGTGTCGGACGGGGGAAGATCTAAATGGCGATCTAACGGGTACGGCGACGAAGGGTATGATTTTGATGACAGCGACAGTGAAATCGCGGTGGGGAAGGAGAATTTGAAAGCGGAGGAAGTTAAGAAACCGAAGGTTAAGAAGGTGAAGAAGCCTAAGGTTACTTTAGCTGAAGCTGCTGCTAAGATTGATGTATCTAATCTCGCAGCTTTCCTCGTTGAAGCTTCGATTCCACTTTCTCATATTCCTGAAGCAGTGTATAAGACATCAGCTGATTGGATTAACCAACGTCCCATTGAGGCACTTGGAGCCTTTGTATTGTGGGGCTTGGATTGTATTCTTGCTGACTTGGCTGTACAACAAGGAGGTGTCAAGGGTGGTAAGAAAGGAGCACAACAGGCTTCTTCGAAATCTCAGGTTGCGATATTTGTGGCGGTAGCAATGGTTTTGCGTAAGAAACCTGATGCTTTAACTAATATATTGCCAACTTTGAGGGAGAATCCCAAGTATCAGGGACAGGATAAGCTTCCAGTTACAGTTTGGATGATGGCTCAGGCCTCCCAAGGTGATATATCAGTAGGCTTGTACTCGTGGGCTCACAACTTGTTACCGGTGGTCAGTAGCAAGAGTTGCAATCCTCAGTCAAGGGATCTCATTCTTCAGTTGGTTGAGAGGATCTTGTCCAACCCGAAGGCCCGGACCATACTTGTAAATGGGGCTGTTAGGAAGGGAGAGCGATTGATTCCACCTCCTTCGTTTGAGATCTTGGTTCGACTTACATTCCCTGCATCTTCCGCAAGAGTGAAGGCTACAGAGAGGTTTGAGGCAATATATCCCTTGCTGAAGGAGGTGTCTCTTGCTGGTGCTCCTGGGAGTAAGGCAATGAAGCAAGTCACACAGCAAATATTCACTTTTGCTCTGAAAGCAGCCGGGGAAGAAAATCCCTTATTAGCCAAGGAAGCGGCTGCAATCACTATCTGGGCTCTCACTCAAAATGTTGATTGCTGCAAGCATTGGGAGAATCTTTATACCGACAATTTGAAAGCCAGTGTTGCTGTTCTTAAAAAGCTCATTGGCGAGTGGAAGGAGCGTTCTGTCAAGCTGACTCCTGCTGAAACCCTCACTCTCAACCAAACAATGAAGAGCTTGAGGCAAAAGAACGAGGAAGCTCTTACTGAGGGAGGTAATGGTGTAAGCCAGTCCCTATACAAAGACGCAGACAAGTACTGCAAGGTGATCGCTGGGAAACTCTCCAGTGGTGGCTGCATCAAAAGTATAGCTACCACTGCTGCACTTCTAGCTGCTACTGGTTTTGCTGGTGCTGCTGCTCTGTCTGCCAACCCTGAGGCTATAGCTTACCTAAAGAACCTGGTGGAGTCATTGGACATCAACAAATTCACCGAGACAGTTATGACAGCATTGAAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

569

Amino Acids

61.39

Weight (kDa)

9.17

Isoelectric Point (pI)

30.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 241 - 517 4.2e-13 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 257, 368
AclWI GGATC 5 cut(s) 12, 132, 241, 936, 962
AcsI RAATTY 2 cut(s) 382, 1673
AcuI CTGAAG 5 cut(s) 249, 471, 549, 923, 1142
AfaI GTAC 7 cut(s) 26, 92, 178, 323, 636, 866, 1505
AgeI ACCGGT 1 cut(s) 889
AgsI TTSAA 6 cut(s) 14, 38, 388, 500, 1321, 1702
AjnI CCWGG 2 cut(s) 1147, 1647
AloI GAACNNNNNNTCC 2 cut(s) 1354, 1386
Alw21I GWGCWC 2 cut(s) 673, 1147
Alw26I GTCTC 2 cut(s) 1137, 1676
AlwI GGATC 5 cut(s) 12, 132, 241, 936, 962
AoxI GGCC 2 cut(s) 834, 972
ApoI RAATTY 2 cut(s) 382, 1673
AsiGI ACCGGT 1 cut(s) 889
AspS9I GGNCC 2 cut(s) 973, 978
AsuC2I CCSGG 3 cut(s) 157, 976, 1210
AsuHPI GGTGA 5 cut(s) 145, 436, 857, 1525, 1669
AsuII TTCGAA 1 cut(s) 686
AvaII GGWCC 1 cut(s) 978
AxyI CCTNAGG 2 cut(s) 435, 1623
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 877, 1264
BauI CACGAG 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 196
Bbv12I GWGCWC 2 cut(s) 673, 1147
BccI CCATC 1 cut(s) 820
BceAI ACGGC 2 cut(s) 291, 340
BcgI CGANNNNNNTGC 2 cut(s) 1498, 1532
BciT130I CCWGG 2 cut(s) 1149, 1649
BcnI CCSGG 3 cut(s) 157, 976, 1210
BcoDI GTCTC 2 cut(s) 1137, 1676
BfaI CTAG 1 cut(s) 1577
BfmI CTRYAG 3 cut(s) 242, 1089, 1629
BglII AGATCT 2 cut(s) 299, 1041
BmcAI AGTACT 1 cut(s) 1505
Bme1390I CCNGG 5 cut(s) 157, 976, 1149, 1210, 1649
Bme18I GGWCC 1 cut(s) 978
BmgT120I GGNCC 2 cut(s) 973, 978
BmiI GGNNCC 2 cut(s) 6, 589
BmrFI CCNGG 5 cut(s) 157, 976, 1149, 1210, 1649
BmsI GCATC 3 cut(s) 733, 1076, 1554
BpiI GAAGAC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 1516
Bpu10I CCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 686
BpuEI CTTGAG 2 cut(s) 236, 1447
BpuMI CCSGG 3 cut(s) 157, 976, 1210
BsaJI CCNNGG 5 cut(s) 70, 841, 1148, 1209, 1233
BsaWI WCCGGW 2 cut(s) 105, 889
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 579, 609
Bse118I RCCGGY 2 cut(s) 118, 889
Bse1I ACTGG 5 cut(s) 809, 1326, 1477, 1533, 1591
Bse21I CCTNAGG 2 cut(s) 435, 1623
Bse3DI GCAATG 2 cut(s) 726, 1167
BseBI CCWGG 2 cut(s) 1149, 1649
BseDI CCNNGG 5 cut(s) 70, 841, 1148, 1209, 1233
BseGI GGATG 2 cut(s) 286, 828
BseMI GCAATG 2 cut(s) 726, 1167
BseMII CTCAG 5 cut(s) 707, 845, 932, 1446, 1614
BseNI ACTGG 5 cut(s) 809, 1326, 1477, 1533, 1591
BseRI GAGGAG 2 cut(s) 207, 233
BseYI CCCAGC 1 cut(s) 1520
BsgI GTGCAG 1 cut(s) 1554
Bsh1236I CGCG 2 cut(s) 257, 368
BshFI GGCC 2 cut(s) 836, 974
BshTI ACCGGT 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 673, 1147
BsiSI CCGG 6 cut(s) 106, 119, 156, 890, 976, 1209
BslFI GGGAC 3 cut(s) 555, 807, 1465
BsmAI GTCTC 2 cut(s) 1137, 1676
BsmFI GGGAC 3 cut(s) 555, 807, 1465
BsnI GGCC 2 cut(s) 836, 974
Bsp119I TTCGAA 1 cut(s) 686
Bsp1286I GDGCHC 4 cut(s) 673, 877, 1147, 1264
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
Bsp19I CCATGG 1 cut(s) 70
BspANI GGCC 2 cut(s) 836, 974
BspCNI CTCAG 5 cut(s) 706, 844, 931, 1447, 1615
BspFNI CGCG 2 cut(s) 257, 368
BspLI GGNNCC 2 cut(s) 6, 589
BspPI GGATC 5 cut(s) 12, 132, 241, 936, 962
BspQI GCTCTTC 1 cut(s) 1415
BspT104I TTCGAA 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 726, 1167
BsrFI RCCGGY 2 cut(s) 118, 889
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 5 cut(s) 809, 1326, 1477, 1533, 1591
BssAI RCCGGY 2 cut(s) 118, 889
BssECI CCNNGG 5 cut(s) 70, 841, 1148, 1209, 1233
BssMI GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
BssSI CACGAG 1 cut(s) 868
BssT1I CCWWGG 3 cut(s) 70, 841, 1233
Bst2BI CACGAG 1 cut(s) 868
Bst2UI CCWGG 2 cut(s) 1149, 1649
Bst4CI ACNGT 3 cut(s) 359, 817, 1687
Bst6I CTCTTC 1 cut(s) 1415
BstAPI GCANNNNNTGC 1 cut(s) 909
BstAUI TGTACA 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 2 cut(s) 225, 1289
BstDEI CTNAG 7 cut(s) 435, 462, 693, 831, 918, 1455, 1623
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 286, 828
BstFNI CGCG 2 cut(s) 257, 368
BstKTI GATC 9 cut(s) 7, 127, 249, 302, 314, 931, 957, 1044, 1518
BstMAI GTCTC 2 cut(s) 1137, 1676
BstMBI GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
BstNI CCWGG 2 cut(s) 1149, 1649
BstSCI CCNGG 5 cut(s) 155, 974, 1147, 1208, 1647
BstSFI CTRYAG 3 cut(s) 242, 1089, 1629
BstUI CGCG 2 cut(s) 257, 368
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 6 cut(s) 4, 246, 299, 928, 954, 1041
BstYI RGATCY 6 cut(s) 4, 246, 299, 928, 954, 1041
Bsu36I CCTNAGG 2 cut(s) 435, 1623
BsuRI GGCC 2 cut(s) 836, 974
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 286, 828
BtsI GCAGTG 2 cut(s) 540, 1563
BtsIMutI CAGTG 6 cut(s) 24, 364, 540, 1333, 1540, 1563
Cac8I GCNNGC 2 cut(s) 225, 1289
Cfr10I RCCGGY 2 cut(s) 118, 889
Cfr13I GGNCC 2 cut(s) 973, 978
CseI GACGC 1 cut(s) 1502
CsiI ACCWGGT 1 cut(s) 1647
Csp6I GTAC 7 cut(s) 25, 91, 177, 322, 635, 865, 1504
CspAI ACCGGT 1 cut(s) 889
CviAII CATG 1 cut(s) 71
CviQI GTAC 7 cut(s) 25, 91, 177, 322, 635, 865, 1504
DdeI CTNAG 7 cut(s) 435, 462, 693, 831, 918, 1455, 1623
DpnI GATC 9 cut(s) 6, 126, 248, 301, 313, 930, 956, 1043, 1517
DpnII GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
Eam1104I CTCTTC 1 cut(s) 1415
EarI CTCTTC 1 cut(s) 1415
EciI GGCGGA 2 cut(s) 137, 293
Eco130I CCWWGG 3 cut(s) 70, 841, 1233
Eco147I AGGCCT 1 cut(s) 836
Eco24I GRGCYC 2 cut(s) 877, 1264
Eco47I GGWCC 1 cut(s) 978
Eco57I CTGAAG 5 cut(s) 249, 471, 549, 923, 1142
Eco81I CCTNAGG 2 cut(s) 435, 1623
EcoRII CCWGG 2 cut(s) 1147, 1647
EcoT14I CCWWGG 3 cut(s) 70, 841, 1233
EcoT38I GRGCYC 2 cut(s) 877, 1264
ErhI CCWWGG 3 cut(s) 70, 841, 1233
FaeI CATG 1 cut(s) 74
FaqI GGGAC 3 cut(s) 555, 807, 1465
FatI CATG 1 cut(s) 70
FokI GGATG 2 cut(s) 293, 835
FriOI GRGCYC 2 cut(s) 877, 1264
FspBI CTAG 1 cut(s) 1577
GsaI CCCAGC 1 cut(s) 1524
GsuI CTGGAG 1 cut(s) 1516
HaeIII GGCC 2 cut(s) 836, 974
HapII CCGG 6 cut(s) 106, 119, 156, 890, 976, 1209
HgaI GACGC 1 cut(s) 1502
Hin1II CATG 1 cut(s) 74
HindIII AAGCTT 3 cut(s) 264, 501, 803
HinfI GANTC 8 cut(s) 14, 160, 508, 778, 1021, 1300, 1384, 1655
HpaII CCGG 6 cut(s) 106, 119, 156, 890, 976, 1209
HphI GGTGA 5 cut(s) 145, 436, 857, 1525, 1669
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 3 cut(s) 271, 289, 1200
Hpy188III TCNNGA 2 cut(s) 215, 527
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 3 cut(s) 68, 142, 332
HpyCH4III ACNGT 3 cut(s) 359, 817, 1687
HpyCH4IV ACGT 3 cut(s) 100, 201, 568
HpyCH4V TGCA 7 cut(s) 912, 1067, 1247, 1287, 1509, 1545, 1571
HpyF3I CTNAG 7 cut(s) 435, 462, 693, 831, 918, 1455, 1623
HpySE526I ACGT 3 cut(s) 100, 201, 568
Hsp92II CATG 1 cut(s) 74
Kzo9I GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
LguI GCTCTTC 1 cut(s) 1415
LmnI GCTCC 5 cut(s) 220, 587, 668, 1150, 1366
LweI GCATC 3 cut(s) 733, 1076, 1554
MabI ACCWGGT 1 cut(s) 1647
MaeI CTAG 1 cut(s) 1577
MaeII ACGT 3 cut(s) 100, 201, 568
MaeIII GTNAC 7 cut(s) 50, 101, 151, 439, 811, 885, 1171
MalI GATC 9 cut(s) 6, 126, 248, 301, 313, 930, 956, 1043, 1517
MboI GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 6 cut(s) 4, 246, 299, 928, 954, 1041
MhlI GDGCHC 4 cut(s) 673, 877, 1147, 1264
MluCI AATT 5 cut(s) 9, 165, 382, 1315, 1673
MlyI GAGTC 2 cut(s) 1378, 1664
MmeI TCCRAC 3 cut(s) 56, 267, 987
MseI TTAA 5 cut(s) 402, 417, 561, 749, 1341
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 6 cut(s) 106, 119, 156, 890, 976, 1209
MspR9I CCNGG 5 cut(s) 157, 976, 1149, 1210, 1649
MunI CAATTG 1 cut(s) 9
MvaI CCWGG 2 cut(s) 1149, 1649
MvnI CGCG 2 cut(s) 257, 368
NciI CCSGG 3 cut(s) 157, 976, 1210
NcoI CCATGG 1 cut(s) 70
NdeII GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 2 cut(s) 6, 589
NmuCI GTSAC 2 cut(s) 151, 1171
NspV TTCGAA 1 cut(s) 686
PceI AGGCCT 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 1415
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 6 cut(s) 14, 160, 508, 778, 1021, 1300
PinAI ACCGGT 1 cut(s) 889
PleI GAGTC 2 cut(s) 1378, 1663
PpsI GAGTC 2 cut(s) 1378, 1663
Psp6I CCWGG 2 cut(s) 1147, 1647
PspFI CCCAGC 1 cut(s) 1520
PspGI CCWGG 2 cut(s) 1147, 1647
PspN4I GGNNCC 2 cut(s) 6, 589
PspPI GGNCC 2 cut(s) 973, 978
PsuI RGATCY 6 cut(s) 4, 246, 299, 928, 954, 1041
PvuII CAGCTG 1 cut(s) 551
RsaI GTAC 7 cut(s) 26, 92, 178, 323, 636, 866, 1505
RsaNI GTAC 7 cut(s) 25, 91, 177, 322, 635, 865, 1504
SapI GCTCTTC 1 cut(s) 1415
SaqAI TTAA 5 cut(s) 402, 417, 561, 749, 1341
Sau3AI GATC 9 cut(s) 4, 124, 246, 299, 311, 928, 954, 1041, 1515
Sau96I GGNCC 2 cut(s) 973, 978
ScaI AGTACT 1 cut(s) 1505
SchI GAGTC 2 cut(s) 1378, 1664
ScrFI CCNGG 5 cut(s) 157, 976, 1149, 1210, 1649
SduI GDGCHC 4 cut(s) 673, 877, 1147, 1264
SexAI ACCWGGT 1 cut(s) 1647
SfaNI GCATC 3 cut(s) 733, 1076, 1554
SfcI CTRYAG 3 cut(s) 242, 1089, 1629
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 1 cut(s) 978
SmlI CTYRAG 2 cut(s) 215, 1426
SmoI CTYRAG 2 cut(s) 215, 1426
Sse9I AATT 5 cut(s) 9, 165, 382, 1315, 1673
SseBI AGGCCT 1 cut(s) 836
SspI AATATT 1 cut(s) 1185
SspMI CTAG 1 cut(s) 1577
StuI AGGCCT 1 cut(s) 836
StyD4I CCNGG 5 cut(s) 155, 974, 1147, 1208, 1647
StyI CCWWGG 3 cut(s) 70, 841, 1233
TaaI ACNGT 3 cut(s) 359, 817, 1687
TaiI ACGT 3 cut(s) 103, 204, 571
TaqI TCGA 3 cut(s) 506, 686, 1051
TasI AATT 5 cut(s) 9, 165, 382, 1315, 1673
TatI WGTACW 4 cut(s) 24, 634, 864, 1503
TauI GCSGC 4 cut(s) 134, 137, 257, 1244
TfiI GAWTC 6 cut(s) 14, 160, 508, 778, 1021, 1300
Tru1I TTAA 5 cut(s) 402, 417, 561, 749, 1341
Tru9I TTAA 5 cut(s) 402, 417, 561, 749, 1341
TscAI CASTG 6 cut(s) 24, 364, 540, 1333, 1540, 1570
TseFI GTSAC 2 cut(s) 151, 1171
Tsp45I GTSAC 2 cut(s) 151, 1171
TspDTI ATGAA 2 cut(s) 1178, 1433
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 6 cut(s) 24, 364, 540, 1333, 1540, 1570
VpaK11BI GGWCC 1 cut(s) 978
XapI RAATTY 2 cut(s) 382, 1673
XcmI CCANNNNNNNNNTGG 1 cut(s) 816
XspI CTAG 1 cut(s) 1577
ZrmI AGTACT 1 cut(s) 1505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.