MD00G1185200.v1.1

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002090
Physical Location & Seq
Reverse (-)
44065542 .. 44070072
4531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1185200.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGGACGAGAATTCGTCGGCACTAATCGAAGCAATTCTCAGAGAGCAAGAGGAGGAGGAGGCACAGAGATACGGCAAGAAGCTGACCCAAAGCGGCGACCCTTACGCCTGGCAAACGGTTTCGTATCCGAAGCGAAACCGGAAATCCTCCTCCAAGGCGATTCCTGCGGATTCCAACGGCGGTCTCCACAACGGTGTCGGATCCTCCGCCTCCGACGTGTTCCGGCCAATCGAGCTGCATTCCGAGGAGCGCCGTCGGAGAGTTATGGAGGCCCAGTCCGCAGCCGTTTCCGGAGACGCCGCACCCGGCGGATCGAAGCGGAATTCAGATGACGACGATGGGGATAATAGCGATGCTGAGGTGGCCGGGGCGGCCGTGGAGAACGGCGAGGTGAAGAAGGTGAAGCAGAAGAAGCCGAAGAAGCCGAAGGTGACGGTGGCGGAGGCGGCGTCGAAAATGGACGCCGGTGATCTGGGCGCCTTTCTGGCAGATATAACTGCATCGTATGAAACGCAGCAGGACATACAGCTTATGCGTTTGGCGGATTATTTCGGCCGAGCATTTGCGGCGGTCAGTCCGGCACAATTTCCGTGGTTGAAGACGTTCAAGGAATCTTCTGTGGCAAAGCTGGTTGATATCCCTCTTTCGCATATATCTGAAGATGTTTACAAGATATCGGTTGAATGGATCGGCCAGCGCTCTACTGAAGCGCTTGGATCATTCGTGCTATGGTCGTTGGACAGCATTCTTGCTGACCTTGCAAGTCATCAAGGAGTAGCCAAGGGATCCAAAAAGGCGGTCCAGCAAGCACCCTTAAAGTCTCAGGTTGCCATATTCGTGGTTTTAGCAATGGTATTGCGACGAAGACCTGAAGTACTGATTAGTTTACTACCTGTGATGAGGGGAACTCCGAAATATGAAGGCCAAGATAAGTTTCCAATTACAGTTTGGCTGGTTGCACAGGCTTCTCAAGGAGATCTGGTTGTGGGGTTGTACTCGTGGGTGCATTTTCTCTTGCCTATATTGAGTAGCAAGTCAAGTAGTAATCCTCTGTGTAGAGATTTAATTTTGCAGTCTGTGGAAAGAATTTTGGCTTTTCCGAAAGCCCGTCCAATTCTGTTAAACGGTGCTGTCAGAAAGGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTCTCTTTTCCAGCACCTTCTGCCCGAGTTAAGGCAACCGAGAGGTTTGAAGCTATCTATCCAACATTGAAAGAGATCGCCCTTGCTGGTTCCCCAGGAAGCAAAACAATGAGGCAAGTCACACAGCAGATATTAAAGAATTCAGTTAAAGCTGTTAATGAAGGCATCCCTGATCTGTCTAAGGAAGCAAGTGGCTTATTTATTTGGTGTTTGACGCAAAACCCTGAATGTTACAGACAATGGGACGTACTTTATCTGGACAATCTCTATGCAAGCGTTGTTCTCCTGAAAAAGCTATCTGACGAGTGGAAGGAGAAGTCTGTTAAACATGCTTCTCTTGACCCCTTGAGGGAAACTTTAAAAAGTTTCAGGGAGAAGAATGATAAGGCATTGGCAGGGGGAGATGATGTTGCCCGCCATTCACTGTTGAAGGATGCAGACAAATATTGCAAGCAGATTTTGGGACGATTGTCGCAAGGCCATGGATGCATGAAGAGCATGGTGCTTGTTTCTGTTGCCCTGGCTGTGGGTGCTGCGGTCATGTCCCAAAACATACAACTCGAGGACCTCAAGAAACTTGCAGCCATGATTAACTTTCCCCCAACTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

599

Amino Acids

65.49

Weight (kDa)

8.92

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 131 - 573 7.1e-23 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 476
AccIII TCCGGA 1 cut(s) 290
AclWI GGATC 7 cut(s) 195, 208, 319, 695, 724, 780, 793
AcoI YGGCCR 5 cut(s) 224, 363, 372, 553, 691
AcsI RAATTY 4 cut(s) 10, 322, 1086, 1324
AcuI CTGAAG 3 cut(s) 678, 726, 891
AcyI GRCGYC 4 cut(s) 297, 449, 462, 477
AdeI CACNNNGTG 1 cut(s) 1153
AfaI GTAC 3 cut(s) 876, 995, 1434
AfeI AGCGCT 2 cut(s) 698, 711
AfiI CCNNNNNNNGG 3 cut(s) 1216, 1534, 1711
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 6 cut(s) 598, 607, 683, 1235, 1255, 1615
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 3 cut(s) 107, 1279, 1704
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 7 cut(s) 82, 235, 529, 628, 1238, 1337, 1480
AluI AGCT 7 cut(s) 82, 235, 529, 628, 1238, 1337, 1480
Alw21I GWGCWC 1 cut(s) 1149
Alw26I GTCTC 4 cut(s) 188, 288, 825, 1192
AlwI GGATC 7 cut(s) 195, 208, 319, 695, 724, 780, 793
Ama87I CYCGRG 2 cut(s) 1209, 1745
Aor13HI TCCGGA 1 cut(s) 290
Aor51HI AGCGCT 2 cut(s) 698, 711
AoxI GGCC 8 cut(s) 224, 270, 363, 372, 553, 691, 922, 1663
ApeKI GCWGC 5 cut(s) 235, 281, 514, 1718, 1766
ApoI RAATTY 4 cut(s) 10, 322, 1086, 1324
ArsI GACNNNNNNTTYG 2 cut(s) 783, 815
Asp700I GAANNNNTTC 2 cut(s) 33, 602
AspLEI GCGC 4 cut(s) 252, 479, 699, 712
AspS9I GGNCC 3 cut(s) 271, 799, 1750
AsuC2I CCSGG 2 cut(s) 306, 367
AsuHPI GGTGA 4 cut(s) 403, 412, 442, 479
AvaI CYCGRG 2 cut(s) 1209, 1745
AvaII GGWCC 2 cut(s) 799, 1750
BamHI GGATCC 2 cut(s) 200, 785
BanI GGYRCC 1 cut(s) 476
BauI CACGAG 1 cut(s) 997
BbsI GAAGAC 2 cut(s) 605, 871
Bbv12I GWGCWC 1 cut(s) 1149
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 5 cut(s) 222, 293, 526, 1705, 1778
BccI CCATC 2 cut(s) 332, 1168
BceAI ACGGC 6 cut(s) 88, 193, 237, 269, 359, 400
BciT130I CCWGG 3 cut(s) 109, 1281, 1706
BciVI GTATCC 1 cut(s) 135
BcnI CCSGG 2 cut(s) 306, 367
BcoDI GTCTC 4 cut(s) 188, 288, 825, 1192
BfoI RGCGCY 4 cut(s) 253, 480, 700, 713
BfuI GTATCC 1 cut(s) 135
BglI GCCNNNNNGGC 2 cut(s) 371, 485
BglII AGATCT 1 cut(s) 976
BmcAI AGTACT 1 cut(s) 876
Bme1390I CCNGG 5 cut(s) 109, 306, 367, 1281, 1706
Bme18I GGWCC 2 cut(s) 799, 1750
BmeT110I CYCGRG 2 cut(s) 1209, 1745
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 3 cut(s) 271, 799, 1750
BmiI GGNNCC 4 cut(s) 202, 478, 787, 1276
BmrFI CCNGG 5 cut(s) 109, 306, 367, 1281, 1706
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 5 cut(s) 343, 509, 1359, 1609, 1661
BmuI ACTGGG 1 cut(s) 268
BoxI GACNNNNGTC 1 cut(s) 1654
BpiI GAAGAC 2 cut(s) 605, 871
BplI GAGNNNNNCTC 2 cut(s) 892, 924
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 3 cut(s) 954, 1552, 1739
BpuMI CCSGG 2 cut(s) 306, 367
BsaHI GRCGYC 4 cut(s) 297, 449, 462, 477
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 9 cut(s) 153, 243, 366, 375, 590, 780, 1279, 1666, 1704
BsaWI WCCGGW 2 cut(s) 138, 290
BsaXI ACNNNNNCTCC 8 cut(s) 260, 290, 434, 464, 966, 996, 1578, 1608
Bsc4I CCNNNNNNNGG 3 cut(s) 1216, 1534, 1711
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 1 cut(s) 274
Bse3DI GCAATG 1 cut(s) 855
BseAI TCCGGA 1 cut(s) 290
BseBI CCWGG 3 cut(s) 109, 1281, 1706
BseDI CCNNGG 9 cut(s) 153, 243, 366, 375, 590, 780, 1279, 1666, 1704
BseGI GGATG 3 cut(s) 1350, 1624, 1676
BseLI CCNNNNNNNGG 3 cut(s) 1216, 1534, 1711
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 3 cut(s) 52, 348, 836
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 5 cut(s) 65, 68, 71, 139, 260
BseX3I CGGCCG 2 cut(s) 372, 553
BseXI GCAGC 5 cut(s) 222, 293, 526, 1705, 1778
Bsh1285I CGRYCG 2 cut(s) 375, 556
BshFI GGCC 8 cut(s) 226, 272, 365, 374, 555, 693, 924, 1665
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 2 cut(s) 375, 556
BsiHKAI GWGCWC 1 cut(s) 1149
BsiHKCI CYCGRG 2 cut(s) 1209, 1745
BsiSI CCGG 7 cut(s) 139, 223, 291, 306, 366, 465, 578
BslFI GGGAC 3 cut(s) 1442, 1662, 1714
BslI CCNNNNNNNGG 3 cut(s) 1216, 1534, 1711
BsmAI GTCTC 4 cut(s) 188, 288, 825, 1192
BsmBI CGTCTC 1 cut(s) 288
BsmFI GGGAC 3 cut(s) 1442, 1662, 1714
BsmI GAATGC 2 cut(s) 238, 744
BsnI GGCC 8 cut(s) 226, 272, 365, 374, 555, 693, 924, 1665
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 2 cut(s) 1209, 1745
Bsp1286I GDGCHC 1 cut(s) 1149
Bsp13I TCCGGA 1 cut(s) 290
Bsp19I CCATGG 1 cut(s) 1666
BspANI GGCC 8 cut(s) 226, 272, 365, 374, 555, 693, 924, 1665
BspCNI CTCAG 3 cut(s) 51, 349, 835
BspEI TCCGGA 1 cut(s) 290
BspLI GGNNCC 4 cut(s) 202, 478, 787, 1276
BspPI GGATC 7 cut(s) 195, 208, 319, 695, 724, 780, 793
BspQI GCTCTTC 1 cut(s) 1673
BspT107I GGYRCC 1 cut(s) 476
BspTNI GGTCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 855
BsrFI RCCGGY 1 cut(s) 464
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 9 cut(s) 153, 243, 366, 375, 590, 780, 1279, 1666, 1704
BssNI GRCGYC 4 cut(s) 297, 449, 462, 477
BssSI CACGAG 1 cut(s) 997
BssT1I CCWWGG 3 cut(s) 153, 780, 1666
Bst2BI CACGAG 1 cut(s) 997
Bst2UI CCWGG 3 cut(s) 109, 1281, 1706
Bst4CI ACNGT 6 cut(s) 118, 194, 436, 946, 1127, 1611
Bst6I CTCTTC 1 cut(s) 1673
BstACI GRCGYC 4 cut(s) 297, 449, 462, 477
BstAPI GCANNNNNTGC 2 cut(s) 1153, 1205
BstC8I GCNNGC 5 cut(s) 695, 807, 1459, 1600, 1637
BstDEI CTNAG 4 cut(s) 38, 357, 822, 1365
BstDSI CCRYGG 3 cut(s) 375, 590, 1666
BstF5I GGATG 3 cut(s) 1350, 1624, 1676
BstH2I RGCGCY 4 cut(s) 253, 480, 700, 713
BstHHI GCGC 4 cut(s) 252, 479, 699, 712
BstMAI GTCTC 4 cut(s) 188, 288, 825, 1192
BstMCI CGRYCG 2 cut(s) 375, 556
BstNI CCWGG 3 cut(s) 109, 1281, 1706
BstNSI RCATGY 1 cut(s) 1517
BstPAI GACNNNNGTC 1 cut(s) 1654
BstSCI CCNGG 5 cut(s) 107, 304, 365, 1279, 1704
BstV1I GCAGC 5 cut(s) 222, 293, 526, 1705, 1778
BstV2I GAAGAC 2 cut(s) 605, 871
BstX2I RGATCY 3 cut(s) 200, 785, 976
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 3 cut(s) 200, 785, 976
BstZI CGGCCG 2 cut(s) 372, 553
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 8 cut(s) 226, 272, 365, 374, 555, 693, 924, 1665
BtgI CCRYGG 3 cut(s) 375, 590, 1666
BtgZI GCGATG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 3 cut(s) 1350, 1624, 1676
BtsIMutI CAGTG 1 cut(s) 1607
Cac8I GCNNGC 5 cut(s) 695, 807, 1459, 1600, 1637
CfoI GCGC 4 cut(s) 252, 479, 699, 712
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 3 cut(s) 271, 799, 1750
CseI GACGC 4 cut(s) 305, 438, 470, 1408
Csp6I GTAC 3 cut(s) 875, 994, 1433
CspCI CAANNNNNGTGG 2 cut(s) 572, 607
CviAII CATG 6 cut(s) 1514, 1667, 1675, 1684, 1726, 1771
CviQI GTAC 3 cut(s) 875, 994, 1433
DdeI CTNAG 4 cut(s) 38, 357, 822, 1365
DinI GGCGCC 1 cut(s) 478
DraI TTTAAA 1 cut(s) 1545
DraIII CACNNNGTG 1 cut(s) 1153
EaeI YGGCCR 5 cut(s) 224, 363, 372, 553, 691
EagI CGGCCG 2 cut(s) 372, 553
Eam1104I CTCTTC 1 cut(s) 1673
EarI CTCTTC 1 cut(s) 1673
EciI GGCGGA 4 cut(s) 196, 324, 455, 557
EclXI CGGCCG 2 cut(s) 372, 553
Eco130I CCWWGG 3 cut(s) 153, 780, 1666
Eco31I GGTCTC 1 cut(s) 188
Eco32I GATATC 2 cut(s) 637, 675
Eco47I GGWCC 2 cut(s) 799, 1750
Eco47III AGCGCT 2 cut(s) 698, 711
Eco52I CGGCCG 2 cut(s) 372, 553
Eco57I CTGAAG 3 cut(s) 678, 726, 891
Eco88I CYCGRG 2 cut(s) 1209, 1745
EcoO109I RGGNCCY 1 cut(s) 1750
EcoRI GAATTC 3 cut(s) 10, 322, 1324
EcoRII CCWGG 3 cut(s) 107, 1279, 1704
EcoRV GATATC 2 cut(s) 637, 675
EcoT14I CCWWGG 3 cut(s) 153, 780, 1666
EcoT22I ATGCAT 1 cut(s) 1676
EgeI GGCGCC 1 cut(s) 478
EheI GGCGCC 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 153, 780, 1666
Esp3I CGTCTC 1 cut(s) 288
FaeI CATG 6 cut(s) 1517, 1670, 1678, 1687, 1729, 1774
FaqI GGGAC 3 cut(s) 1442, 1662, 1714
FatI CATG 6 cut(s) 1513, 1666, 1674, 1683, 1725, 1770
FauI CCCGC 1 cut(s) 1607
FokI GGATG 3 cut(s) 1337, 1631, 1683
GlaI GCGC 4 cut(s) 251, 478, 698, 711
HaeII RGCGCY 4 cut(s) 253, 480, 700, 713
HaeIII GGCC 8 cut(s) 226, 272, 365, 374, 555, 693, 924, 1665
HapII CCGG 7 cut(s) 139, 223, 291, 306, 366, 465, 578
HgaI GACGC 4 cut(s) 305, 438, 470, 1408
HhaI GCGC 4 cut(s) 252, 479, 699, 712
Hin1I GRCGYC 4 cut(s) 297, 449, 462, 477
Hin1II CATG 6 cut(s) 1517, 1670, 1678, 1687, 1729, 1774
Hin6I GCGC 4 cut(s) 250, 477, 697, 710
HinP1I GCGC 4 cut(s) 250, 477, 697, 710
HinfI GANTC 4 cut(s) 160, 170, 611, 1185
HpaII CCGG 7 cut(s) 139, 223, 291, 306, 366, 465, 578
HphI GGTGA 4 cut(s) 403, 412, 442, 479
Hpy166II GTNNAC 2 cut(s) 667, 887
Hpy188III TCNNGA 6 cut(s) 291, 1177, 1442, 1471, 1523, 1756
Hpy8I GTNNAC 2 cut(s) 667, 887
Hpy99I CGWCG 6 cut(s) 19, 218, 258, 338, 454, 864
HpyAV CCTTC 7 cut(s) 391, 421, 914, 1212, 1340, 1489, 1609
HpyCH4III ACNGT 6 cut(s) 118, 194, 436, 946, 1127, 1611
HpyCH4IV ACGT 3 cut(s) 216, 602, 1431
HpyF3I CTNAG 4 cut(s) 38, 357, 822, 1365
HpySE526I ACGT 3 cut(s) 216, 602, 1431
Hsp92I GRCGYC 4 cut(s) 297, 449, 462, 477
Hsp92II CATG 6 cut(s) 1517, 1670, 1678, 1687, 1729, 1774
HspAI GCGC 4 cut(s) 250, 477, 697, 710
KasI GGCGCC 1 cut(s) 476
Kpn2I TCCGGA 1 cut(s) 290
LguI GCTCTTC 1 cut(s) 1673
LmnI GCTCC 2 cut(s) 247, 1144
Lsp1109I GCAGC 5 cut(s) 222, 293, 526, 1705, 1778
LweI GCATC 5 cut(s) 343, 509, 1359, 1609, 1661
MaeII ACGT 3 cut(s) 216, 602, 1431
MaeIII GTNAC 3 cut(s) 430, 1303, 1415
MboII GAAGA 9 cut(s) 406, 421, 430, 606, 610, 671, 876, 1573, 1690
MflI RGATCY 3 cut(s) 200, 785, 976
MhlI GDGCHC 1 cut(s) 1149
MluCI AATT 9 cut(s) 10, 33, 322, 584, 939, 1065, 1086, 1113, 1324
Mly113I GGCGCC 1 cut(s) 477
MlyI GAGTC 1 cut(s) 1194
MmeI TCCRAC 6 cut(s) 178, 198, 236, 237, 717, 1271
Mph1103I ATGCAT 1 cut(s) 1676
MroI TCCGGA 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 33, 602
MslI CAYNNNNRTG 2 cut(s) 192, 836
MspI CCGG 7 cut(s) 139, 223, 291, 306, 366, 465, 578
MspR9I CCNGG 5 cut(s) 109, 306, 367, 1281, 1706
Mva1269I GAATGC 2 cut(s) 238, 744
MvaI CCWGG 3 cut(s) 109, 1281, 1706
NarI GGCGCC 1 cut(s) 477
NciI CCSGG 2 cut(s) 306, 367
NcoI CCATGG 1 cut(s) 1666
NlaIII CATG 6 cut(s) 1517, 1670, 1678, 1687, 1729, 1774
NlaIV GGNNCC 4 cut(s) 202, 478, 787, 1276
NmeAIII GCCGAG 1 cut(s) 581
NmuCI GTSAC 2 cut(s) 430, 1303
NsiI ATGCAT 1 cut(s) 1676
NspI RCATGY 1 cut(s) 1517
OliI CACNNNNGTG 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 1745
PciSI GCTCTTC 1 cut(s) 1673
PctI GAATGC 2 cut(s) 238, 744
PdmI GAANNNNTTC 2 cut(s) 33, 602
PfeI GAWTC 3 cut(s) 160, 170, 611
PleI GAGTC 1 cut(s) 1193
PluTI GGCGCC 1 cut(s) 480
PpsI GAGTC 1 cut(s) 1193
PpuMI RGGWCCY 1 cut(s) 1750
PshAI GACNNNNGTC 1 cut(s) 1654
Psp5II RGGWCCY 1 cut(s) 1750
Psp6I CCWGG 3 cut(s) 107, 1279, 1704
PspGI CCWGG 3 cut(s) 107, 1279, 1704
PspN4I GGNNCC 4 cut(s) 202, 478, 787, 1276
PspPI GGNCC 3 cut(s) 271, 799, 1750
PspPPI RGGWCCY 1 cut(s) 1750
PspXI VCTCGAGB 1 cut(s) 1745
PsuI RGATCY 3 cut(s) 200, 785, 976
RsaI GTAC 3 cut(s) 876, 995, 1434
RsaNI GTAC 3 cut(s) 875, 994, 1433
RseI CAYNNNNRTG 2 cut(s) 192, 836
SapI GCTCTTC 1 cut(s) 1673
Sau96I GGNCC 3 cut(s) 271, 799, 1750
ScaI AGTACT 1 cut(s) 876
SchI GAGTC 1 cut(s) 1194
ScrFI CCNGG 5 cut(s) 109, 306, 367, 1281, 1706
SduI GDGCHC 1 cut(s) 1149
SfaNI GCATC 5 cut(s) 343, 509, 1359, 1609, 1661
SfiI GGCCNNNNNGGCC 1 cut(s) 371
SfoI GGCGCC 1 cut(s) 478
Sfr274I CTCGAG 1 cut(s) 1745
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 2 cut(s) 799, 1750
SlaI CTCGAG 1 cut(s) 1745
SmiMI CAYNNNNRTG 2 cut(s) 192, 836
SmlI CTYRAG 4 cut(s) 969, 1531, 1745, 1754
SmoI CTYRAG 4 cut(s) 969, 1531, 1745, 1754
Sse9I AATT 9 cut(s) 10, 33, 322, 584, 939, 1065, 1086, 1113, 1324
SspDI GGCGCC 1 cut(s) 476
SspI AATATT 1 cut(s) 1631
StyD4I CCNGG 5 cut(s) 107, 304, 365, 1279, 1704
StyI CCWWGG 3 cut(s) 153, 780, 1666
TaaI ACNGT 6 cut(s) 118, 194, 436, 946, 1127, 1611
TaiI ACGT 3 cut(s) 219, 605, 1434
TaqI TCGA 5 cut(s) 27, 231, 314, 452, 1746
TasI AATT 9 cut(s) 10, 33, 322, 584, 939, 1065, 1086, 1113, 1324
TatI WGTACW 2 cut(s) 874, 993
TauI GCSGC 5 cut(s) 96, 302, 374, 449, 569
TfiI GAWTC 3 cut(s) 160, 170, 611
TscAI CASTG 1 cut(s) 1614
TseFI GTSAC 2 cut(s) 430, 1303
TseI GCWGC 5 cut(s) 235, 281, 514, 1718, 1766
Tsp45I GTSAC 2 cut(s) 430, 1303
TspDTI ATGAA 4 cut(s) 522, 933, 1359, 1691
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 1 cut(s) 1614
VpaK11BI GGWCC 2 cut(s) 799, 1750
XapI RAATTY 4 cut(s) 10, 322, 1086, 1324
XceI RCATGY 1 cut(s) 1517
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XhoI CTCGAG 1 cut(s) 1745
XmnI GAANNNNTTC 2 cut(s) 33, 602
ZrmI AGTACT 1 cut(s) 876
Zsp2I ATGCAT 1 cut(s) 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.