RLG00000022728

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002090
Physical Location & Seq
Reverse (-)
10721756 .. 10726182
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022728

Sequence Viewer

Length: 1758 bp
ATGGACGAGAATTCAGCACTGATCGAATCGATTCTGAGAGAGGAAGACGAGGCGGAGGCAGACAAATATTTAAAGAAGATGAGCAGCACTGCACCAGTCCAAAACGCCGACGCTTACGCCTGGCAAACTGTTTCTTATCACAAGCGAAACCGACGACTTTCTCAGCCAATTCCGCCTGATTCTGTCGGCGATCCCTCCAACGGCGACGTCTTCCGCTCGATCGAGCTGCATTCCGAGGAGCGCCGGAGGAGAATTCGACAGGCTCAAACGGATTCCCTTCCCGCTGTCCGATCCGAGCCGCATTCCGACGACGATAGCGATGCCGAGGTCGCGGCTGCCGCCGAAAATGGCGAGCCGAAGAAGGCCAAAGTGAAGAAGCCGAAGAAGCCCAAGCTGACTGTGGCCGAGGCCGCTTCCAAGATCGACGCGGCTCATCTCGGTGCTTTTCTCGCCGATATAACGGAGGTGTATCAATCCCAGCAAGACATACTGCTTTTGCGTCTGGCTGATTATTTCGGTCGAGCATTTGCGTCCGTCACTGCCGCTCAGTTCCCCTGGTTGAAGATATTCAAGGAATCCACTGTCTCAAAGATGGTTGACATCCCTCTTTCTCATATATCTGAAAATGTTTATAAGACATCAGTTGACTGGATCGGCCAGCGCTCCACTGAAGCCCTTGGGTCCTTCATGTTGTGGTCACTAGATTGCATTCTTGCTGACCTTGCAAGTCATCATGGAGCAGCAAAGGGATCCAAAAAGGTGGTCCAGCAACCTTCAAGATGTCAGGTTGCCATATTCGCGGTTTTAGCAATGGTGTTGCGAAAAAAACCTGATGTATTGTTTAGTCTATTTCCTGTGATGAGGGAAAGTTCGAAATATCAAGGCCAAGATAAGCTTCCGCTCACAGTGTGGTTGATTACGCAGGCCTCTCAAGGGGATCTGGTTGTTGGGTTGTACACATGGATACATCTTCTCCTGCCTATATTGAGTAGCAAGTCAGGTTGTAATCCACAGTCTAGAGACTTGATTTTGCAGTCAGTGGAAAGAATTTTATCATCTCCAAAAGCTCGGCCTATTCTATTAAATGGTGCTGTTAGGAAAGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTTTCTTTTCCTTCACCTTCAGCGCGAGTTAAGGCAACTGAGAGATTTGAAGCTGTCTATCCAACCTTGAAAGAGATTGCCCTTGCTGGATCCCCAGGAAGCAAAACAGTGAGGCAGGTTGCACAGAAGACACTGAATTATTCAGTTAAAGCTGTTAAAGAAGGCATCCCCGATCTCTCTAAGGAGGCAACTGCTATATTTATCTGGTGTTTGACACAAAGTCCCGAATGTTACAAGCAATGGGACATGCTTTATCTGGACAATCTTGATGCAAGTGTTATTGTGCTGAAAAAGTTATCTGACGAGTGGAAGGAGCACTCCGTTAAACATGCCTCTCTTGACCCCTTGAGGGAAACCTTGAAGAGTTTCAGGGAGAAGAATGAAAAAGCATTGGAAAAAGAAGATGATTTGGTGCGCCATTCGTTGTTGAAGGATGCAGATAAATATTGCAAGCTGATTTTAGGACGGTCATCTCAAGGCCGTGGATGCATGAAAAGCATGGTTCTGATGTCTGTTGCCCTTGCTATGGGTGCTGCTATCATGTCCCAAAACATACAACCCAAAGACCTCAAGAAACTTGCAGCCATGTTTAACTTCCCCCTGACTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

586

Amino Acids

65.08

Weight (kDa)

8.99

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 88 - 565 8e-25 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 1252
AccBSI CCGCTC 3 cut(s) 216, 545, 901
AccII CGCG 4 cut(s) 332, 428, 800, 1171
AclWI GGATC 8 cut(s) 185, 285, 659, 744, 757, 945, 1230, 1243
AcoI YGGCCR 2 cut(s) 402, 655
AcsI RAATTY 3 cut(s) 10, 252, 1047
AcuI CTGAAG 2 cut(s) 690, 1149
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 2 cut(s) 909, 1114
AfaI GTAC 1 cut(s) 956
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 4 cut(s) 200, 933, 1495, 1672
AgsI TTSAA 7 cut(s) 562, 571, 777, 1196, 1216, 1507, 1576
AhdI GACNNNNNGTC 1 cut(s) 1365
AjnI CCWGG 3 cut(s) 119, 554, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 7 cut(s) 226, 394, 895, 1067, 1199, 1298, 1600
AluI AGCT 7 cut(s) 226, 394, 895, 1067, 1199, 1298, 1600
Alw21I GWGCWC 2 cut(s) 1110, 1464
Alw26I GTCTC 2 cut(s) 589, 1014
AlwI GGATC 8 cut(s) 185, 285, 659, 744, 757, 945, 1230, 1243
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 8 cut(s) 363, 402, 408, 655, 883, 924, 1070, 1624
ApeKI GCWGC 6 cut(s) 84, 226, 335, 740, 1679, 1727
ApoI RAATTY 3 cut(s) 10, 252, 1047
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 3 cut(s) 30, 566, 1511
AspLEI GCGC 4 cut(s) 243, 663, 1171, 1563
AspS9I GGNCC 2 cut(s) 681, 763
AsuHPI GGTGA 1 cut(s) 1152
AsuII TTCGAA 1 cut(s) 872
AvaII GGWCC 2 cut(s) 681, 763
BamHI GGATCC 2 cut(s) 749, 1235
BbsI GAAGAC 3 cut(s) 51, 202, 1280
Bbv12I GWGCWC 2 cut(s) 1110, 1464
BbvI GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
BccI CCATC 2 cut(s) 586, 1129
BceAI ACGGC 2 cut(s) 217, 1611
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 302, 336
BciT130I CCWGG 3 cut(s) 121, 556, 1242
BciVI GTATCC 1 cut(s) 957
BcoDI GTCTC 2 cut(s) 589, 1014
BfaI CTAG 2 cut(s) 701, 1017
BfoI RGCGCY 2 cut(s) 244, 664
BfuAI ACCTGC 1 cut(s) 1252
BfuI GTATCC 1 cut(s) 957
Bme1390I CCNGG 3 cut(s) 121, 556, 1242
Bme18I GGWCC 2 cut(s) 681, 763
BmeRI GACNNNNNGTC 1 cut(s) 1365
BmgT120I GGNCC 2 cut(s) 681, 763
BmiI GGNNCC 3 cut(s) 682, 751, 1237
BmrFI CCNGG 3 cut(s) 121, 556, 1242
BmsI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
BpiI GAAGAC 3 cut(s) 51, 202, 1280
Bpu14I TTCGAA 1 cut(s) 872
BpuEI CTTGAG 4 cut(s) 915, 1513, 1605, 1700
Bsa29I ATCGAT 1 cut(s) 29
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 7 cut(s) 234, 324, 405, 554, 676, 1240, 1627
BsaXI ACNNNNNCTCC 2 cut(s) 957, 987
Bsc4I CCNNNNNNNGG 4 cut(s) 200, 933, 1495, 1672
Bse1I ACTGG 2 cut(s) 95, 653
Bse3DI GCAATG 2 cut(s) 816, 1391
BseBI CCWGG 3 cut(s) 121, 556, 1242
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 7 cut(s) 234, 324, 405, 554, 676, 1240, 1627
BseGI GGATG 4 cut(s) 600, 1311, 1585, 1637
BseLI CCNNNNNNNGG 4 cut(s) 200, 933, 1495, 1672
BseMI GCAATG 2 cut(s) 816, 1391
BseMII CTCAG 4 cut(s) 26, 176, 560, 1176
BseNI ACTGG 2 cut(s) 95, 653
BseRI GAGGAG 2 cut(s) 251, 262
BseXI GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
BseYI CCCAGC 1 cut(s) 477
BsgI GTGCAG 1 cut(s) 75
Bsh1236I CGCG 4 cut(s) 332, 428, 800, 1171
Bsh1285I CGRYCG 2 cut(s) 222, 520
BshFI GGCC 8 cut(s) 365, 404, 410, 657, 885, 926, 1072, 1626
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 222, 520
BsiHKAI GWGCWC 2 cut(s) 1110, 1464
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 3 cut(s) 1353, 1403, 1675
BslI CCNNNNNNNGG 4 cut(s) 200, 933, 1495, 1672
BsmAI GTCTC 2 cut(s) 589, 1014
BsmFI GGGAC 3 cut(s) 1353, 1403, 1675
BsmI GAATGC 3 cut(s) 229, 301, 708
BsnI GGCC 8 cut(s) 365, 404, 410, 657, 885, 926, 1072, 1626
Bsp119I TTCGAA 1 cut(s) 872
Bsp1286I GDGCHC 2 cut(s) 1110, 1464
Bsp1407I TGTACA 1 cut(s) 954
BspANI GGCC 8 cut(s) 365, 404, 410, 657, 885, 926, 1072, 1626
BspCNI CTCAG 4 cut(s) 27, 175, 559, 1177
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 4 cut(s) 332, 428, 800, 1171
BspLI GGNNCC 3 cut(s) 682, 751, 1237
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 8 cut(s) 185, 285, 659, 744, 757, 945, 1230, 1243
BspT104I TTCGAA 1 cut(s) 872
BsrBI CCGCTC 3 cut(s) 216, 545, 901
BsrDI GCAATG 2 cut(s) 816, 1391
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 2 cut(s) 95, 653
BssECI CCNNGG 7 cut(s) 234, 324, 405, 554, 676, 1240, 1627
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 3 cut(s) 121, 556, 1242
Bst4CI ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
Bst6I CTCTTC 1 cut(s) 1502
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 1114
BstAUI TGTACA 1 cut(s) 954
BstBI TTCGAA 1 cut(s) 872
BstC8I GCNNGC 4 cut(s) 353, 659, 924, 1598
BstDEI CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
BstDSI CCRYGG 1 cut(s) 1627
BstF5I GGATG 4 cut(s) 600, 1311, 1585, 1637
BstFNI CGCG 4 cut(s) 332, 428, 800, 1171
BstH2I RGCGCY 2 cut(s) 244, 664
BstHHI GCGC 4 cut(s) 243, 663, 1171, 1563
BstMAI GTCTC 2 cut(s) 589, 1014
BstMCI CGRYCG 2 cut(s) 222, 520
BstNI CCWGG 3 cut(s) 121, 556, 1242
BstNSI RCATGY 2 cut(s) 1396, 1478
BstSCI CCNGG 3 cut(s) 119, 554, 1240
BstUI CGCG 4 cut(s) 332, 428, 800, 1171
BstV1I GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
BstV2I GAAGAC 3 cut(s) 51, 202, 1280
BstX2I RGATCY 3 cut(s) 749, 937, 1235
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 3 cut(s) 749, 937, 1235
Bsu15I ATCGAT 1 cut(s) 29
BsuI GTATCC 1 cut(s) 957
BsuRI GGCC 8 cut(s) 365, 404, 410, 657, 885, 926, 1072, 1626
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 1627
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 600, 1311, 1585, 1637
BtsI GCAGTG 2 cut(s) 87, 537
BtsIMutI CAGTG 9 cut(s) 17, 87, 537, 579, 666, 912, 1044, 1260, 1277
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 4 cut(s) 353, 659, 924, 1598
CfoI GCGC 4 cut(s) 243, 663, 1171, 1563
Cfr13I GGNCC 2 cut(s) 681, 763
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 4 cut(s) 119, 434, 488, 519
Csp6I GTAC 1 cut(s) 955
CviAII CATG 9 cut(s) 688, 734, 960, 1393, 1475, 1636, 1645, 1687, 1732
CviQI GTAC 1 cut(s) 955
DdeI CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
DraI TTTAAA 1 cut(s) 72
DraIII CACNNNGTG 2 cut(s) 909, 1114
DriI GACNNNNNGTC 1 cut(s) 1365
EaeI YGGCCR 2 cut(s) 402, 655
Eam1104I CTCTTC 1 cut(s) 1502
Eam1105I GACNNNNNGTC 1 cut(s) 1365
EarI CTCTTC 1 cut(s) 1502
EciI GGCGGA 2 cut(s) 68, 162
Eco130I CCWWGG 1 cut(s) 676
Eco147I AGGCCT 1 cut(s) 926
Eco47I GGWCC 2 cut(s) 681, 763
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 690, 1149
EcoO109I RGGNCCY 1 cut(s) 681
EcoRI GAATTC 2 cut(s) 10, 252
EcoRII CCWGG 3 cut(s) 119, 554, 1240
EcoT14I CCWWGG 1 cut(s) 676
EcoT22I ATGCAT 1 cut(s) 1637
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 9 cut(s) 691, 737, 963, 1396, 1478, 1639, 1648, 1690, 1735
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 3 cut(s) 1353, 1403, 1675
FatI CATG 9 cut(s) 687, 733, 959, 1392, 1474, 1635, 1644, 1686, 1731
FauI CCCGC 1 cut(s) 289
FokI GGATG 4 cut(s) 587, 1298, 1592, 1644
FspBI CTAG 2 cut(s) 701, 1017
GlaI GCGC 4 cut(s) 242, 662, 1170, 1562
GsaI CCCAGC 1 cut(s) 481
HaeII RGCGCY 2 cut(s) 244, 664
HaeIII GGCC 8 cut(s) 365, 404, 410, 657, 885, 926, 1072, 1626
HapII CCGG 1 cut(s) 244
HgaI GACGC 4 cut(s) 119, 434, 488, 519
HhaI GCGC 4 cut(s) 243, 663, 1171, 1563
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 9 cut(s) 691, 737, 963, 1396, 1478, 1639, 1648, 1690, 1735
Hin6I GCGC 4 cut(s) 241, 661, 1169, 1561
HinP1I GCGC 4 cut(s) 241, 661, 1169, 1561
HincII GTYRAC 2 cut(s) 598, 646
HindII GTYRAC 2 cut(s) 598, 646
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 6 cut(s) 26, 31, 179, 272, 575, 1750
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 1152
Hpy166II GTNNAC 3 cut(s) 598, 646, 957
Hpy188I TCNGA 8 cut(s) 36, 235, 290, 295, 307, 622, 1447, 1653
Hpy188III TCNNGA 8 cut(s) 777, 1017, 1138, 1370, 1403, 1412, 1484, 1717
Hpy8I GTNNAC 3 cut(s) 598, 646, 957
Hpy99I CGWCG 6 cut(s) 113, 156, 209, 311, 314, 428
HpyAV CCTTC 9 cut(s) 287, 355, 694, 783, 1167, 1173, 1301, 1450, 1570
HpyCH4III ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
HpyCH4IV ACGT 1 cut(s) 207
HpyF3I CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 9 cut(s) 691, 737, 963, 1396, 1478, 1639, 1648, 1690, 1735
HspAI GCGC 4 cut(s) 241, 661, 1169, 1561
LmnI GCTCC 5 cut(s) 238, 668, 737, 1105, 1459
Lsp1109I GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
LweI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
MaeI CTAG 2 cut(s) 701, 1017
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 535, 696, 1376
MbiI CCGCTC 3 cut(s) 216, 545, 901
MflI RGATCY 3 cut(s) 749, 937, 1235
MhlI GDGCHC 2 cut(s) 1110, 1464
MluCI AATT 5 cut(s) 10, 168, 252, 1047, 1282
MlyI GAGTC 1 cut(s) 1744
MmeI TCCRAC 3 cut(s) 222, 330, 1232
Mph1103I ATGCAT 1 cut(s) 1637
MroXI GAANNNNTTC 3 cut(s) 30, 566, 1511
MseI TTAA 7 cut(s) 71, 1082, 1176, 1293, 1302, 1470, 1737
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 3 cut(s) 121, 556, 1242
Mva1269I GAATGC 3 cut(s) 229, 301, 708
MvaI CCWGG 3 cut(s) 121, 556, 1242
MvnI CGCG 4 cut(s) 332, 428, 800, 1171
NlaIII CATG 9 cut(s) 691, 737, 963, 1396, 1478, 1639, 1648, 1690, 1735
NlaIV GGNNCC 3 cut(s) 682, 751, 1237
NmeAIII GCCGAG 3 cut(s) 349, 430, 1048
NmuCI GTSAC 2 cut(s) 535, 696
NsiI ATGCAT 1 cut(s) 1637
NspI RCATGY 2 cut(s) 1396, 1478
NspV TTCGAA 1 cut(s) 872
PceI AGGCCT 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 3 cut(s) 229, 301, 708
PdmI GAANNNNTTC 3 cut(s) 30, 566, 1511
PfeI GAWTC 5 cut(s) 26, 31, 179, 272, 575
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 1 cut(s) 1744
PpsI GAGTC 1 cut(s) 1744
PpuMI RGGWCCY 1 cut(s) 681
PsiI TTATAA 1 cut(s) 633
Psp5II RGGWCCY 1 cut(s) 681
Psp6I CCWGG 3 cut(s) 119, 554, 1240
PspFI CCCAGC 1 cut(s) 477
PspGI CCWGG 3 cut(s) 119, 554, 1240
PspN4I GGNNCC 3 cut(s) 682, 751, 1237
PspPI GGNCC 2 cut(s) 681, 763
PspPPI RGGWCCY 1 cut(s) 681
PsuI RGATCY 3 cut(s) 749, 937, 1235
PvuI CGATCG 1 cut(s) 222
RsaI GTAC 1 cut(s) 956
RsaNI GTAC 1 cut(s) 955
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 7 cut(s) 71, 1082, 1176, 1293, 1302, 1470, 1737
Sau96I GGNCC 2 cut(s) 681, 763
SchI GAGTC 1 cut(s) 1744
ScrFI CCNGG 3 cut(s) 121, 556, 1242
SduI GDGCHC 2 cut(s) 1110, 1464
SfaNI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
SfuI TTCGAA 1 cut(s) 872
SinI GGWCC 2 cut(s) 681, 763
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 4 cut(s) 930, 1492, 1620, 1715
SmoI CTYRAG 4 cut(s) 930, 1492, 1620, 1715
Sse9I AATT 5 cut(s) 10, 168, 252, 1047, 1282
SseBI AGGCCT 1 cut(s) 926
SspI AATATT 2 cut(s) 68, 1592
SspMI CTAG 2 cut(s) 701, 1017
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 3 cut(s) 119, 554, 1240
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
TaiI ACGT 1 cut(s) 210
TaqI TCGA 8 cut(s) 24, 29, 218, 222, 256, 423, 520, 872
TaqII GACCGA 1 cut(s) 506
TasI AATT 5 cut(s) 10, 168, 252, 1047, 1282
TatI WGTACW 1 cut(s) 954
TauI GCSGC 6 cut(s) 301, 335, 341, 413, 431, 545
TfiI GAWTC 5 cut(s) 26, 31, 179, 272, 575
Tru1I TTAA 7 cut(s) 71, 1082, 1176, 1293, 1302, 1470, 1737
Tru9I TTAA 7 cut(s) 71, 1082, 1176, 1293, 1302, 1470, 1737
TscAI CASTG 9 cut(s) 24, 94, 544, 586, 673, 912, 1044, 1260, 1284
TseFI GTSAC 2 cut(s) 535, 696
TseI GCWGC 6 cut(s) 84, 226, 335, 740, 1679, 1727
Tsp45I GTSAC 2 cut(s) 535, 696
TspDTI ATGAA 3 cut(s) 676, 1542, 1652
TspGWI ACGGA 4 cut(s) 284, 476, 523, 1456
TspRI CASTG 9 cut(s) 24, 94, 544, 586, 673, 912, 1044, 1260, 1284
VpaK11BI GGWCC 2 cut(s) 681, 763
XapI RAATTY 3 cut(s) 10, 252, 1047
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 2 cut(s) 1396, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XmnI GAANNNNTTC 3 cut(s) 30, 566, 1511
XspI CTAG 2 cut(s) 701, 1017
ZraI GACGTC 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.