Rh3CG341200

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002090
Physical Location & Seq
Forward (+)
38408616 .. 38413985
5370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG341200.1

Sequence Viewer

Length: 1353 bp
ATGGACGAGAATTCAGCACTGATCGAATCGATTCTGAGAGAGGAAGACGAGGCGGAGGCAGACAAATATGTAAAGAAGATGAGCAGCACTGCGCCAGTCCAAAACGGCGACGCTTACGCCTGGCAAACTGTTTCTTATCACAAGAGAAACCGACGACTTTCTCAGCCAATTCCGCCTGATTCTGTCGGCGATCCCTCCAACGGCGACGTCTTCCGCTCGATCGAGCTGCATTCCGAGGACCGCCGGAGGAGAATTCGACAGGCTCAAACGGATTCCCTTCCCGCTGTCCGATCCGAGCTGCATTCCGACGACGATAGCGATGCCGAGGTGGCCGCTGCCGCGGAAAATGGCGAGCCGAAGAAGGCCAAAGTGAAGAAGCCGAAGAAGCCAAAGCTGACTGTGGCCGAGGCGGCTTCCAAGATCGACGCGGCTCATCTCGGTGCTTTTCTCGCCGAGATAACGGAGGTGTATGAATCCCAACAAGACATACTGCTTTTGCGTCTGGCTGATTATTTCGGTCGAGCATTTGCGTCCGTCACCGCCGCTCAGTTTCCCTGGTTGAAGATATTCAAGGAATCCACCGTCTCAAAGATGGTTGACATCCCTCTTTCTCATATATCTGAAAATGTTTATAAGACATCAGCTGACTGGATCGGCCAGCGCTCCACTGAAGCCCTTGGGTCCTTCGTGTTGTGGTCACTAGATTGCATTCTTGCTGACCTTGCAAGTCATCAGGGAGCAGCAAAGGGATCCAAAAAGGTGGTCCAGCAACCTTCAAAATGTCAGGTTGCCATATTCACGGTTTTAGCAATGGTGTTGCGAAAAAAACCTGATGTATTGTTTAGTCTATTTCCTGTGATGAGGGAAAGTTCGAAATATCAAGGCCAAGATAAGCTTCCGCTCACAGTGTGGTTGATTACGCAGGCCTCTCAAGGAGATCTGGTTGTTGGGTTGTACACATGGGTACATCTTCTCCTGCCTATATTGAGTAGCAAGTCAGGTTGTAATCCACAGTCTAGAGACTTGATTTTGCAGTCAGTAGAAAGAATTTTATCATCTCCAAAAGCTCGGCCTATTCTATTAAATGGTGCTGTTAGGAAAGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTTTCTTTTCCTTCACCTTCAGCGCGAGTTAAGGCAACTGAGAGATTTGAAGCTGTCTATCCAACCTTGAAAGAGATTGCCCTTGCTGGGTCCCCAGGAAGCAAAACAGTGAAGCAGGTTGCACAGAAGACACTGAATTATTCAGTTAAAGCTGTTAAAGAAGGTATAACCAATAGCAAAGAAACTATTTTAAATTTATTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

450

Amino Acids

49.59

Weight (kDa)

8.93

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 97 - 418 2.3e-07 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 1252
AccBSI CCGCTC 3 cut(s) 216, 545, 901
AccII CGCG 3 cut(s) 341, 428, 1171
AclWI GGATC 5 cut(s) 185, 285, 659, 744, 757
AcoI YGGCCR 3 cut(s) 330, 402, 655
AcsI RAATTY 4 cut(s) 10, 252, 1047, 1339
AcuI CTGAAG 2 cut(s) 690, 1149
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 2 cut(s) 909, 1114
AfaI GTAC 2 cut(s) 956, 966
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 2 cut(s) 200, 1233
AgsI TTSAA 5 cut(s) 562, 571, 777, 1196, 1216
AjnI CCWGG 3 cut(s) 119, 554, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 8 cut(s) 226, 298, 394, 644, 895, 1067, 1199, 1298
AluI AGCT 8 cut(s) 226, 298, 394, 644, 895, 1067, 1199, 1298
Alw21I GWGCWC 1 cut(s) 1110
Alw26I GTCTC 2 cut(s) 589, 1014
AlwI GGATC 5 cut(s) 185, 285, 659, 744, 757
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 7 cut(s) 330, 363, 402, 655, 883, 924, 1070
ApeKI GCWGC 5 cut(s) 84, 226, 298, 335, 740
ApoI RAATTY 4 cut(s) 10, 252, 1047, 1339
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 2 cut(s) 30, 566
AspLEI GCGC 3 cut(s) 94, 663, 1171
AspS9I GGNCC 4 cut(s) 238, 681, 763, 1236
AsuHPI GGTGA 2 cut(s) 529, 1152
AsuII TTCGAA 1 cut(s) 872
AvaII GGWCC 4 cut(s) 238, 681, 763, 1236
BamHI GGATCC 1 cut(s) 749
BbsI GAAGAC 3 cut(s) 51, 202, 1280
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 5 cut(s) 96, 213, 285, 322, 752
BccI CCATC 2 cut(s) 586, 1129
BceAI ACGGC 2 cut(s) 121, 217
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 302, 336
BciT130I CCWGG 3 cut(s) 121, 556, 1242
BcoDI GTCTC 2 cut(s) 589, 1014
BfaI CTAG 2 cut(s) 701, 1017
BfoI RGCGCY 1 cut(s) 664
BfuAI ACCTGC 1 cut(s) 1252
BglI GCCNNNNNGGC 2 cut(s) 329, 410
BglII AGATCT 1 cut(s) 937
Bme1390I CCNGG 3 cut(s) 121, 556, 1242
Bme18I GGWCC 4 cut(s) 238, 681, 763, 1236
BmgT120I GGNCC 4 cut(s) 238, 681, 763, 1236
BmiI GGNNCC 4 cut(s) 682, 751, 1237, 1238
BmrFI CCNGG 3 cut(s) 121, 556, 1242
BmsI GCATC 1 cut(s) 310
BpiI GAAGAC 3 cut(s) 51, 202, 1280
Bpu14I TTCGAA 1 cut(s) 872
BpuEI CTTGAG 1 cut(s) 915
Bsa29I ATCGAT 1 cut(s) 29
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1240
BsaXI ACNNNNNCTCC 4 cut(s) 927, 957, 957, 987
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 1233
Bse1I ACTGG 2 cut(s) 95, 653
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 3 cut(s) 121, 556, 1242
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1240
BseGI GGATG 1 cut(s) 600
BseLI CCNNNNNNNGG 2 cut(s) 200, 1233
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 4 cut(s) 26, 176, 560, 1176
BseNI ACTGG 2 cut(s) 95, 653
BseRI GAGGAG 1 cut(s) 262
BseXI GCAGC 5 cut(s) 96, 213, 285, 322, 752
BseYI CCCAGC 1 cut(s) 1232
Bsh1236I CGCG 3 cut(s) 341, 428, 1171
Bsh1285I CGRYCG 2 cut(s) 222, 520
BshFI GGCC 7 cut(s) 332, 365, 404, 657, 885, 926, 1072
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 222, 520
BsiHKAI GWGCWC 1 cut(s) 1110
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 1222
BslI CCNNNNNNNGG 2 cut(s) 200, 1233
BsmAI GTCTC 2 cut(s) 589, 1014
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 1222
BsmI GAATGC 3 cut(s) 229, 301, 708
BsnI GGCC 7 cut(s) 332, 365, 404, 657, 885, 926, 1072
Bsp119I TTCGAA 1 cut(s) 872
Bsp1286I GDGCHC 1 cut(s) 1110
Bsp1407I TGTACA 1 cut(s) 954
Bsp143I GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
BspANI GGCC 7 cut(s) 332, 365, 404, 657, 885, 926, 1072
BspCNI CTCAG 4 cut(s) 27, 175, 559, 1177
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 3 cut(s) 341, 428, 1171
BspLI GGNNCC 4 cut(s) 682, 751, 1237, 1238
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 5 cut(s) 185, 285, 659, 744, 757
BspT104I TTCGAA 1 cut(s) 872
BsrBI CCGCTC 3 cut(s) 216, 545, 901
BsrDI GCAATG 1 cut(s) 816
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 2 cut(s) 95, 653
BssECI CCNNGG 7 cut(s) 234, 324, 339, 405, 554, 676, 1240
BssMI GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 3 cut(s) 121, 556, 1242
Bst4CI ACNGT 7 cut(s) 130, 400, 583, 802, 907, 1014, 1255
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 1114
BstAUI TGTACA 1 cut(s) 954
BstBI TTCGAA 1 cut(s) 872
BstC8I GCNNGC 3 cut(s) 353, 659, 924
BstDEI CTNAG 4 cut(s) 35, 162, 546, 1185
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 600
BstFNI CGCG 3 cut(s) 341, 428, 1171
BstH2I RGCGCY 1 cut(s) 664
BstHHI GCGC 3 cut(s) 94, 663, 1171
BstKTI GATC 9 cut(s) 24, 193, 222, 293, 423, 654, 752, 940, 1135
BstMAI GTCTC 2 cut(s) 589, 1014
BstMBI GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
BstMCI CGRYCG 2 cut(s) 222, 520
BstMWI GCNNNNNNNGC 8 cut(s) 172, 329, 338, 385, 410, 449, 722, 1114
BstNI CCWGG 3 cut(s) 121, 556, 1242
BstSCI CCNGG 3 cut(s) 119, 554, 1240
BstUI CGCG 3 cut(s) 341, 428, 1171
BstV1I GCAGC 5 cut(s) 96, 213, 285, 322, 752
BstV2I GAAGAC 3 cut(s) 51, 202, 1280
BstX2I RGATCY 2 cut(s) 749, 937
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 2 cut(s) 749, 937
Bsu15I ATCGAT 1 cut(s) 29
BsuRI GGCC 7 cut(s) 332, 365, 404, 657, 885, 926, 1072
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 339
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 600
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 6 cut(s) 17, 87, 666, 912, 1260, 1277
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 3 cut(s) 353, 659, 924
CfoI GCGC 3 cut(s) 94, 663, 1171
Cfr13I GGNCC 4 cut(s) 238, 681, 763, 1236
Cfr42I CCGCGG 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 4 cut(s) 119, 434, 488, 519
Csp6I GTAC 2 cut(s) 955, 965
CviAII CATG 1 cut(s) 960
CviQI GTAC 2 cut(s) 955, 965
DdeI CTNAG 4 cut(s) 35, 162, 546, 1185
DpnI GATC 9 cut(s) 23, 192, 221, 292, 422, 653, 751, 939, 1134
DpnII GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
DraI TTTAAA 1 cut(s) 1338
DraIII CACNNNGTG 2 cut(s) 909, 1114
EaeI YGGCCR 3 cut(s) 330, 402, 655
EciI GGCGGA 2 cut(s) 68, 162
Eco130I CCWWGG 1 cut(s) 676
Eco147I AGGCCT 1 cut(s) 926
Eco47I GGWCC 4 cut(s) 238, 681, 763, 1236
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 690, 1149
EcoO109I RGGNCCY 2 cut(s) 681, 1236
EcoRI GAATTC 2 cut(s) 10, 252
EcoRII CCWGG 3 cut(s) 119, 554, 1240
EcoT14I CCWWGG 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 676
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 1 cut(s) 963
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 1 cut(s) 1222
FatI CATG 1 cut(s) 959
FauI CCCGC 1 cut(s) 289
FokI GGATG 1 cut(s) 587
FspBI CTAG 2 cut(s) 701, 1017
GlaI GCGC 3 cut(s) 93, 662, 1170
GsaI CCCAGC 1 cut(s) 1236
HaeII RGCGCY 1 cut(s) 664
HaeIII GGCC 7 cut(s) 332, 365, 404, 657, 885, 926, 1072
HapII CCGG 1 cut(s) 244
HgaI GACGC 4 cut(s) 119, 434, 488, 519
HhaI GCGC 3 cut(s) 94, 663, 1171
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 1 cut(s) 963
Hin6I GCGC 3 cut(s) 92, 661, 1169
HinP1I GCGC 3 cut(s) 92, 661, 1169
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 6 cut(s) 26, 31, 179, 272, 473, 575
HpaII CCGG 1 cut(s) 244
HphI GGTGA 2 cut(s) 529, 1152
Hpy166II GTNNAC 2 cut(s) 598, 957
Hpy188I TCNGA 6 cut(s) 36, 235, 290, 295, 307, 622
Hpy188III TCNNGA 2 cut(s) 1017, 1138
Hpy8I GTNNAC 2 cut(s) 598, 957
Hpy99I CGWCG 6 cut(s) 113, 156, 209, 311, 314, 428
HpyAV CCTTC 7 cut(s) 287, 355, 694, 783, 1167, 1173, 1301
HpyCH4III ACNGT 7 cut(s) 130, 400, 583, 802, 907, 1014, 1255
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 6 cut(s) 229, 301, 708, 725, 1033, 1268
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 329, 338, 385, 410, 449, 722, 1114
HpyF3I CTNAG 4 cut(s) 35, 162, 546, 1185
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 1 cut(s) 963
HspAI GCGC 3 cut(s) 92, 661, 1169
KflI GGGWCCC 1 cut(s) 1236
KspI CCGCGG 1 cut(s) 342
Kzo9I GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
LmnI GCTCC 3 cut(s) 668, 737, 1105
Lsp1109I GCAGC 5 cut(s) 96, 213, 285, 322, 752
LweI GCATC 1 cut(s) 310
MaeI CTAG 2 cut(s) 701, 1017
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 2 cut(s) 535, 696
MalI GATC 9 cut(s) 23, 192, 221, 292, 422, 653, 751, 939, 1134
MbiI CCGCTC 3 cut(s) 216, 545, 901
MboI GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
MboII GAAGA 9 cut(s) 56, 88, 202, 370, 385, 394, 574, 962, 1285
MflI RGATCY 2 cut(s) 749, 937
MhlI GDGCHC 1 cut(s) 1110
MluCI AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1339
MmeI TCCRAC 3 cut(s) 222, 330, 1232
MroXI GAANNNNTTC 2 cut(s) 30, 566
MseI TTAA 5 cut(s) 1082, 1176, 1293, 1302, 1337
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 4 cut(s) 284, 335, 341, 644
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 3 cut(s) 121, 556, 1242
Mva1269I GAATGC 3 cut(s) 229, 301, 708
MvaI CCWGG 3 cut(s) 121, 556, 1242
MvnI CGCG 3 cut(s) 341, 428, 1171
MwoI GCNNNNNNNGC 8 cut(s) 172, 329, 338, 385, 410, 449, 722, 1114
NdeII GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
NlaIII CATG 1 cut(s) 963
NlaIV GGNNCC 4 cut(s) 682, 751, 1237, 1238
NmeAIII GCCGAG 4 cut(s) 349, 430, 478, 1048
NmuCI GTSAC 2 cut(s) 535, 696
NspV TTCGAA 1 cut(s) 872
PceI AGGCCT 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 3 cut(s) 229, 301, 708
PdmI GAANNNNTTC 2 cut(s) 30, 566
PfeI GAWTC 6 cut(s) 26, 31, 179, 272, 473, 575
Ple19I CGATCG 1 cut(s) 222
PpuMI RGGWCCY 2 cut(s) 681, 1236
PsiI TTATAA 1 cut(s) 633
Psp5II RGGWCCY 2 cut(s) 681, 1236
Psp6I CCWGG 3 cut(s) 119, 554, 1240
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 3 cut(s) 119, 554, 1240
PspN4I GGNNCC 4 cut(s) 682, 751, 1237, 1238
PspPI GGNCC 4 cut(s) 238, 681, 763, 1236
PspPPI RGGWCCY 2 cut(s) 681, 1236
PsuI RGATCY 2 cut(s) 749, 937
PvuI CGATCG 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 956, 966
RsaNI GTAC 2 cut(s) 955, 965
RseI CAYNNNNRTG 1 cut(s) 438
SacII CCGCGG 1 cut(s) 342
SaqAI TTAA 5 cut(s) 1082, 1176, 1293, 1302, 1337
Sau3AI GATC 9 cut(s) 21, 190, 219, 290, 420, 651, 749, 937, 1132
Sau96I GGNCC 4 cut(s) 238, 681, 763, 1236
ScrFI CCNGG 3 cut(s) 121, 556, 1242
SduI GDGCHC 1 cut(s) 1110
SfaNI GCATC 1 cut(s) 310
Sfr303I CCGCGG 1 cut(s) 342
SfuI TTCGAA 1 cut(s) 872
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 4 cut(s) 238, 681, 763, 1236
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 930
SmoI CTYRAG 1 cut(s) 930
Sse9I AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1339
SseBI AGGCCT 1 cut(s) 926
SspMI CTAG 2 cut(s) 701, 1017
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 3 cut(s) 119, 554, 1240
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 7 cut(s) 130, 400, 583, 802, 907, 1014, 1255
TaiI ACGT 1 cut(s) 210
TaqI TCGA 8 cut(s) 24, 29, 218, 222, 256, 423, 520, 872
TaqII GACCGA 1 cut(s) 506
TasI AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1339
TatI WGTACW 1 cut(s) 954
TauI GCSGC 5 cut(s) 335, 341, 413, 431, 545
TfiI GAWTC 6 cut(s) 26, 31, 179, 272, 473, 575
Tru1I TTAA 5 cut(s) 1082, 1176, 1293, 1302, 1337
Tru9I TTAA 5 cut(s) 1082, 1176, 1293, 1302, 1337
TscAI CASTG 6 cut(s) 24, 94, 673, 912, 1260, 1284
TseFI GTSAC 2 cut(s) 535, 696
TseI GCWGC 5 cut(s) 84, 226, 298, 335, 740
Tsp45I GTSAC 2 cut(s) 535, 696
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 3 cut(s) 284, 476, 523
TspRI CASTG 6 cut(s) 24, 94, 673, 912, 1260, 1284
VpaK11BI GGWCC 4 cut(s) 238, 681, 763, 1236
XapI RAATTY 4 cut(s) 10, 252, 1047, 1339
XbaI TCTAGA 1 cut(s) 1016
XmnI GAANNNNTTC 2 cut(s) 30, 566
XspI CTAG 2 cut(s) 701, 1017
ZraI GACGTC 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.