AT1G70770

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002090
Physical Location & Seq
Reverse (-)
26687798 .. 26691281
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70770.1

Sequence Viewer

Length: 1833 bp
ATGGATCCGATCGAATCTGTCGAGTACAATGGCTTCGAGACCTCTAACGGTAACGTCGACCATGGTTGGAAGAAAGTCGTGTACCCGAAACGTCACCGGAAGCAAAAACAAGCGGATCAAGCGACGGCAAATGGTGGCAAAACTGTTGCTTCAAATGGAACCGTAGCTAACGGCGGAGATAACGTTTTCCGTTCGTTGGAAGAGCAAGCTGAGGATCGTCGACGACGGATCTTAGCGGCGAAGATGACTGCTATTGATTCAGATGATGATGGTGTTGTTAGATCTAAACATCGATCTAATGGTTACGGTGATGATGGGTATGATTTTGATGGTAGTGATGATGAGATCGCAACGCTGAAGGTTGAGGAAGTTAAGAAGCCAAAGCCTAAGAAGGAGAAGAAGCCTAAAGTTTCTTTGCCTGAAGCTGCATCAAAGATCGATCCTTTAAATCTCGAAGCTTTTCTCGTTGAAGCATCGGAATCGTATGCGAGTCAGCCTGAGATTCAGCTAATGAGATTTGCTGATTACTTTGGGAGAGCACTTTCTGGAGTCTCATCTGTGCAATTTCCATGGGTGAAGATGTTTAAAGAGTCTCCTTTGTCTAAGCTGATTGAAGTTCCGTTAGCTCATATTCCGGAACCAGTGTATAAGACATCAGTTGATTGGATCAACCATCGTCCAATTGAGGCACTTGGAGCCTTTGTGTTGTGGGCTTTTGATTGTATTCTCACAGATTTAGCAGCACAACAAGGTGGTGCTAAAGGTGGGAAGAAGGGTGGTCAACAAACTACTTCCAAATCTCAGGTGGCAATATTTGTTGCATTAGCGATGGTATTGCGTAGGAAGCCTGATGCTTTGACTAATGTATTGCCAACTCTGAGGGAGAATCCTAAGTATCAGGGTCAGGATAAGCTTCCGGTTACAGTTTGGATGATGGCTCAGGCCTCCCAAGGTGATATAGCTGTAGGCTTGTATTCATGGGCTCACAACTTGTTACCTGTTGTCGGTAATAAGAACTGTAACCCTCAGTCAAGGGATCTCATCCTGCAGTTGGTTGAGAAAATTTTGACGAATCCAAAGGCTCGTACCATACTTGTAAATGGAGCTGTTAGGAAGGGAGAGCGTTTGATTCCACCTCCTTCATTTGAGATCTTGTTGCGGCTTACATTCCCTGCATCATCTGCAAGAGTAAAGGCTACAGAGAGGTTTGAGGCAATATATCCCTTGCTGAAAGAAGTGGCTCTTGCTGGTGCACCAGGAAGCAAGGCGATGAAACAAGTCACACAACAGATATTCACTTTTGCTCTGAAATTAGCTGGAGAAGGAAATCCTGTTTTAGCCAAGGAAGCTACAGCAATTGCTATCTGGTCTGTGACCCAAAACTTTGATTGCTGCAAGCACTGGGACAATCTCTACAAGGAGAATCTGGAAGCTAGTGTTGCTGTTCTTAAAAAGCTTGTTGAAGAATGGAAAGAGCATTCGGTCAAACTATCATCATCACCGAATGATGCTCTCACTCTCAACCGAACTATGAAAAGCTTCAGGCTAAAGAACGAGGAGGTCATCACTGAGGGAGGAGCCAATGCTTCTCTTTACAAAGAAGCTGACAAGTCTTGCAAAACGATCTCGGGGAGACTCTCCCGTGGAAGTGGCTGCCTCAAAGGAACAGCCATTACTTTAGTGTTTCTAGCTGCTGCAGGAGCCGCTGCTGCAGCTGTTCTATCTTCCAACCCGGAGGTTACAAACGAGCTGAAGAACCTGGTGGACTCATTGGAGCTGCATGAATACATCAACAAATACACTGAGGTTATCTCCACAGCTTTGAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

610

Amino Acids

66.86

Weight (kDa)

8.98

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 275 - 568 2.5e-12 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 57, 220
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 5 cut(s) 113, 174, 236, 1159, 1704
AclI AACGTT 1 cut(s) 183
AclWI GGATC 7 cut(s) 12, 123, 222, 236, 434, 674, 1044
AcsI RAATTY 1 cut(s) 1062
AcuI CTGAAG 4 cut(s) 377, 441, 1525, 1772
AfaI GTAC 3 cut(s) 26, 83, 1087
AfiI CCNNNNNNNGG 3 cut(s) 196, 1004, 1051
AgsI TTSAA 5 cut(s) 153, 470, 614, 1463, 1825
AjnI CCWGG 2 cut(s) 1255, 1758
Alw21I GWGCWC 2 cut(s) 541, 1255
Alw26I GTCTC 4 cut(s) 32, 556, 597, 1627
Alw44I GTGCAC 1 cut(s) 1251
AlwI GGATC 7 cut(s) 12, 123, 222, 236, 434, 674, 1044
Ama87I CYCGRG 1 cut(s) 1627
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 1 cut(s) 942
ApaLI GTGCAC 1 cut(s) 1251
ApoI RAATTY 1 cut(s) 1062
Asp700I GAANNNNTTC 2 cut(s) 459, 1538
AsuC2I CCSGG 1 cut(s) 1733
AsuHPI GGTGA 5 cut(s) 86, 320, 586, 965, 1491
AvaI CYCGRG 1 cut(s) 1627
BaeGI GKGCMC 1 cut(s) 1255
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 985
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
Bbv12I GWGCWC 2 cut(s) 541, 1255
BbvCI CCTCAGC 1 cut(s) 210
BccI CCATC 6 cut(s) 263, 308, 323, 681, 823, 928
BceAI ACGGC 2 cut(s) 141, 187
BcgI CGANNNNNNTGC 4 cut(s) 462, 496, 817, 851
BciT130I CCWGG 2 cut(s) 1257, 1760
BcnI CCSGG 1 cut(s) 1733
BcoDI GTCTC 4 cut(s) 32, 556, 597, 1627
BfaI CTAG 2 cut(s) 1434, 1688
BfmI CTRYAG 6 cut(s) 963, 1046, 1197, 1350, 1695, 1710
BglII AGATCT 2 cut(s) 281, 1149
Bme1390I CCNGG 3 cut(s) 1257, 1733, 1760
BmeT110I CYCGRG 1 cut(s) 1627
BmiI GGNNCC 6 cut(s) 6, 160, 639, 697, 1579, 1702
BmrFI CCNGG 3 cut(s) 1257, 1733, 1760
BmrI ACTGGG 1 cut(s) 1411
BmsI GCATC 5 cut(s) 437, 482, 841, 1184, 1498
BmuI ACTGGG 1 cut(s) 1411
BplI GAGNNNNNCTC 2 cut(s) 1796, 1828
BpmI CTGGAG 2 cut(s) 567, 1338
Bpu10I CCTNAGC 2 cut(s) 210, 939
BpuMI CCSGG 1 cut(s) 1733
Bsa29I ATCGAT 2 cut(s) 292, 438
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 5 cut(s) 61, 569, 949, 1341, 1642
BsaWI WCCGGW 3 cut(s) 96, 634, 916
BsaXI ACNNNNNCTCC 4 cut(s) 168, 198, 687, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 1004, 1051
Bse1I ACTGG 2 cut(s) 641, 1406
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 2 cut(s) 1257, 1760
BseCI ATCGAT 2 cut(s) 292, 438
BseDI CCNNGG 5 cut(s) 61, 569, 949, 1341, 1642
BseGI GGATG 2 cut(s) 936, 1041
BseLI CCNNNNNNNGG 3 cut(s) 196, 1004, 1051
BseMII CTCAG 8 cut(s) 201, 489, 815, 869, 953, 1040, 1560, 1794
BseNI ACTGG 2 cut(s) 641, 1406
BseRI GAGGAG 2 cut(s) 1571, 1590
BseSI GKGCMC 1 cut(s) 1255
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 944
BshVI ATCGAT 2 cut(s) 292, 438
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 541, 1255
BsiHKCI CYCGRG 1 cut(s) 1627
BsiSI CCGG 4 cut(s) 97, 635, 917, 1733
BslFI GGGAC 1 cut(s) 1418
BslI CCNNNNNNNGG 3 cut(s) 196, 1004, 1051
BsmAI GTCTC 4 cut(s) 32, 556, 597, 1627
BsmFI GGGAC 1 cut(s) 1418
BsmI GAATGC 1 cut(s) 1477
BsnI GGCC 1 cut(s) 944
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 1627
Bsp1286I GDGCHC 3 cut(s) 541, 985, 1255
Bsp13I TCCGGA 1 cut(s) 634
Bsp19I CCATGG 2 cut(s) 61, 569
BspACI CCGC 5 cut(s) 113, 174, 236, 1159, 1704
BspANI GGCC 1 cut(s) 944
BspCNI CTCAG 8 cut(s) 202, 490, 814, 870, 952, 1039, 1561, 1795
BspDI ATCGAT 2 cut(s) 292, 438
BspEI TCCGGA 1 cut(s) 634
BspLI GGNNCC 6 cut(s) 6, 160, 639, 697, 1579, 1702
BspMAI CTGCAG 3 cut(s) 1050, 1699, 1714
BspPI GGATC 7 cut(s) 12, 123, 222, 236, 434, 674, 1044
BspQI GCTCTTC 1 cut(s) 195
BspTNI GGTCTC 1 cut(s) 32
BsrI ACTGG 2 cut(s) 641, 1406
BssECI CCNNGG 5 cut(s) 61, 569, 949, 1341, 1642
BssT1I CCWWGG 4 cut(s) 61, 569, 949, 1341
Bst2UI CCWGG 2 cut(s) 1257, 1760
Bst4CI ACNGT 6 cut(s) 50, 145, 163, 308, 925, 1019
Bst6I CTCTTC 1 cut(s) 195
BstAPI GCANNNNNTGC 1 cut(s) 1181
BstC8I GCNNGC 2 cut(s) 207, 1397
BstDSI CCRYGG 3 cut(s) 61, 569, 1642
BstF5I GGATG 2 cut(s) 936, 1041
BstMAI GTCTC 4 cut(s) 32, 556, 597, 1627
BstMCI CGRYCG 1 cut(s) 12
BstNI CCWGG 2 cut(s) 1257, 1760
BstSCI CCNGG 3 cut(s) 1255, 1731, 1758
BstSFI CTRYAG 6 cut(s) 963, 1046, 1197, 1350, 1695, 1710
BstSLI GKGCMC 1 cut(s) 1255
BstX2I RGATCY 5 cut(s) 4, 228, 281, 1036, 1149
BstYI RGATCY 5 cut(s) 4, 228, 281, 1036, 1149
Bsu15I ATCGAT 2 cut(s) 292, 438
BsuRI GGCC 1 cut(s) 944
BsuTUI ATCGAT 2 cut(s) 292, 438
BtgI CCRYGG 3 cut(s) 61, 569, 1642
BtgZI GCGATG 2 cut(s) 842, 1283
BtsCI GGATG 2 cut(s) 936, 1041
BtsIMutI CAGTG 4 cut(s) 648, 1399, 1566, 1800
Cac8I GCNNGC 2 cut(s) 207, 1397
ClaI ATCGAT 2 cut(s) 292, 438
CsiI ACCWGGT 1 cut(s) 1758
Csp6I GTAC 3 cut(s) 25, 82, 1086
CviAII CATG 4 cut(s) 62, 570, 978, 1781
CviQI GTAC 3 cut(s) 25, 82, 1086
DraI TTTAAA 2 cut(s) 447, 586
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 189
Eco130I CCWWGG 4 cut(s) 61, 569, 949, 1341
Eco147I AGGCCT 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 985
Eco31I GGTCTC 1 cut(s) 32
Eco57I CTGAAG 4 cut(s) 377, 441, 1525, 1772
Eco88I CYCGRG 1 cut(s) 1627
EcoRII CCWGG 2 cut(s) 1255, 1758
EcoT14I CCWWGG 4 cut(s) 61, 569, 949, 1341
EcoT38I GRGCYC 1 cut(s) 985
ErhI CCWWGG 4 cut(s) 61, 569, 949, 1341
FaeI CATG 4 cut(s) 65, 573, 981, 1784
FalI AAGNNNNNCTT 2 cut(s) 1227, 1259
FaqI GGGAC 1 cut(s) 1418
FatI CATG 4 cut(s) 61, 569, 977, 1780
FblI GTMKAC 2 cut(s) 57, 220
FokI GGATG 2 cut(s) 943, 1028
FriOI GRGCYC 1 cut(s) 985
FspBI CTAG 2 cut(s) 1434, 1688
GsuI CTGGAG 2 cut(s) 567, 1338
HaeIII GGCC 1 cut(s) 944
HapII CCGG 4 cut(s) 97, 635, 917, 1733
Hin1II CATG 4 cut(s) 65, 573, 981, 1784
HincII GTYRAC 3 cut(s) 58, 221, 782
HindII GTYRAC 3 cut(s) 58, 221, 782
HindIII AAGCTT 4 cut(s) 456, 911, 1454, 1537
HpaII CCGG 4 cut(s) 97, 635, 917, 1733
HphI GGTGA 5 cut(s) 86, 320, 586, 965, 1491
Hpy166II GTNNAC 6 cut(s) 58, 82, 221, 782, 1253, 1765
Hpy188I TCNGA 5 cut(s) 9, 262, 478, 879, 1308
Hpy188III TCNNGA 6 cut(s) 37, 452, 546, 635, 905, 1427
Hpy8I GTNNAC 6 cut(s) 58, 82, 221, 782, 1253, 1765
Hpy99I CGWCG 5 cut(s) 59, 127, 222, 225, 228
HpyAV CCTTC 6 cut(s) 352, 385, 766, 1108, 1149, 1316
HpyCH4III ACNGT 6 cut(s) 50, 145, 163, 308, 925, 1019
HpyCH4IV ACGT 3 cut(s) 54, 91, 183
HpySE526I ACGT 3 cut(s) 54, 91, 183
Hsp92II CATG 4 cut(s) 65, 573, 981, 1784
Kpn2I TCCGGA 1 cut(s) 634
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 5 cut(s) 695, 1103, 1577, 1700, 1774
LweI GCATC 5 cut(s) 437, 482, 841, 1184, 1498
MabI ACCWGGT 1 cut(s) 1758
MaeI CTAG 2 cut(s) 1434, 1688
MaeII ACGT 3 cut(s) 54, 91, 183
MaeIII GTNAC 9 cut(s) 50, 92, 302, 919, 993, 1019, 1279, 1372, 1738
MboII GAAGA 9 cut(s) 82, 212, 253, 409, 589, 781, 1475, 1716, 1765
MfeI CAATTG 2 cut(s) 681, 1356
MflI RGATCY 5 cut(s) 4, 228, 281, 1036, 1149
MhlI GDGCHC 3 cut(s) 541, 985, 1255
MluCI AATT 5 cut(s) 563, 681, 1062, 1310, 1356
MlyI GAGTC 5 cut(s) 499, 558, 599, 1629, 1760
MmeI TCCRAC 3 cut(s) 47, 177, 1752
MroI TCCGGA 1 cut(s) 634
MroXI GAANNNNTTC 2 cut(s) 459, 1538
MseI TTAA 4 cut(s) 372, 446, 585, 1449
MspA1I CMGCKG 2 cut(s) 1706, 1715
MspI CCGG 4 cut(s) 97, 635, 917, 1733
MspR9I CCNGG 3 cut(s) 1257, 1733, 1760
MunI CAATTG 2 cut(s) 681, 1356
Mva1269I GAATGC 1 cut(s) 1477
MvaI CCWGG 2 cut(s) 1257, 1760
NciI CCSGG 1 cut(s) 1733
NcoI CCATGG 2 cut(s) 61, 569
NlaIII CATG 4 cut(s) 65, 573, 981, 1784
NlaIV GGNNCC 6 cut(s) 6, 160, 639, 697, 1579, 1702
NmuCI GTSAC 3 cut(s) 92, 1279, 1372
PceI AGGCCT 1 cut(s) 944
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 54
PctI GAATGC 1 cut(s) 1477
PdmI GAANNNNTTC 2 cut(s) 459, 1538
PfeI GAWTC 8 cut(s) 14, 257, 479, 502, 886, 1072, 1129, 1423
Ple19I CGATCG 1 cut(s) 12
PleI GAGTC 5 cut(s) 498, 557, 598, 1629, 1760
PpsI GAGTC 5 cut(s) 498, 557, 598, 1629, 1760
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 2 cut(s) 1255, 1758
PspGI CCWGG 2 cut(s) 1255, 1758
PspN4I GGNNCC 6 cut(s) 6, 160, 639, 697, 1579, 1702
PsrI GAACNNNNNNTAC 2 cut(s) 1657, 1689
PstI CTGCAG 3 cut(s) 1050, 1699, 1714
PsuI RGATCY 5 cut(s) 4, 228, 281, 1036, 1149
PvuI CGATCG 1 cut(s) 12
PvuII CAGCTG 1 cut(s) 1715
RsaI GTAC 3 cut(s) 26, 83, 1087
RsaNI GTAC 3 cut(s) 25, 82, 1086
SalI GTCGAC 2 cut(s) 56, 219
SapI GCTCTTC 1 cut(s) 195
SaqAI TTAA 4 cut(s) 372, 446, 585, 1449
SchI GAGTC 5 cut(s) 499, 558, 599, 1629, 1760
ScrFI CCNGG 3 cut(s) 1257, 1733, 1760
SduI GDGCHC 3 cut(s) 541, 985, 1255
SexAI ACCWGGT 1 cut(s) 1758
SfaNI GCATC 5 cut(s) 437, 482, 841, 1184, 1498
SfcI CTRYAG 6 cut(s) 963, 1046, 1197, 1350, 1695, 1710
SgrDI CGTCGACG 1 cut(s) 219
Sse9I AATT 5 cut(s) 563, 681, 1062, 1310, 1356
SseBI AGGCCT 1 cut(s) 944
SsiI CCGC 5 cut(s) 113, 174, 236, 1159, 1704
SspI AATATT 1 cut(s) 813
SspMI CTAG 2 cut(s) 1434, 1688
StuI AGGCCT 1 cut(s) 944
StyD4I CCNGG 3 cut(s) 1255, 1731, 1758
StyI CCWWGG 4 cut(s) 61, 569, 949, 1341
TaaI ACNGT 6 cut(s) 50, 145, 163, 308, 925, 1019
TaiI ACGT 3 cut(s) 57, 94, 186
TaqI TCGA 8 cut(s) 12, 21, 36, 57, 220, 292, 438, 453
TaqII GACCGA 1 cut(s) 1471
TasI AATT 5 cut(s) 563, 681, 1062, 1310, 1356
TatI WGTACW 1 cut(s) 24
TauI GCSGC 3 cut(s) 239, 1162, 1706
TfiI GAWTC 8 cut(s) 14, 257, 479, 502, 886, 1072, 1129, 1423
Tru1I TTAA 4 cut(s) 372, 446, 585, 1449
Tru9I TTAA 4 cut(s) 372, 446, 585, 1449
TscAI CASTG 4 cut(s) 648, 1406, 1573, 1807
TseFI GTSAC 3 cut(s) 92, 1279, 1372
Tsp45I GTSAC 3 cut(s) 92, 1279, 1372
TspDTI ATGAA 5 cut(s) 966, 1131, 1286, 1547, 1797
TspGWI ACGGA 3 cut(s) 179, 241, 609
TspRI CASTG 4 cut(s) 648, 1406, 1573, 1807
VneI GTGCAC 1 cut(s) 1251
XapI RAATTY 1 cut(s) 1062
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmiI GTMKAC 2 cut(s) 57, 220
XmnI GAANNNNTTC 2 cut(s) 459, 1538
XspI CTAG 2 cut(s) 1434, 1688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.