FvH4_1g28730

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002090
Physical Location & Seq
Forward (+)
21045928 .. 21051043
5116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28730.t1

Sequence Viewer

Length: 1737 bp
ATGGATGTGAATTCAGCTCTGATCGAATCGATTCTGAGAGAGCAAGACGAGGCCGAGGCGGACAAGTATGTTAAGAAGATGGCCAGCACCGCACCGGTCCAAAACGGCGACGCTTACGCCTGGCAGACTGTTTCTTATCACAAGCGAAACCGACGACCCTCCCGCCCAATTCCGCCGGATTCCTTCGCTGATCTCCCCACCGCCGACGTCTTCCGCTCCATTGAGCTGCATTCCGAGGACCGCCGGAGGAGAATCCGAGAGGCTCAAAATGGCGATGACGGTAGCGATGCTGAGGTCGCCGGCGCCGCGGAAAATGGCGAGCCGAAGAAGGTAAAATTGAAGAAGCCGAAGATGACTGTGGCCGAGGGCGCTTCCAAGATCGACTCGGCTCATCTCGGTGCTTTTCTTGCCGAGATAACGGAGGTGTATCAATCGCAGCAAGACATACAGCTTTTGCGTCTGGCTGATTATTTCGGCCGAGCATTTTCGTCCGTTTGTGCGCCTCAGTTTCCCTGGTTCAAGATATTCAAGGAGTCCACTGTCTCAAAGATGGTTGAGATCCCTCTTTCTCATATATCTGAAAATGTTTATAAGACATCAGTTGACTGGATCGGCCAGCGCTCCACTGAAGCCTTAGGGTCCTTCGTGTTATGGTCTCTAGATTGCATTCTTGCTGACCTTTCAAATCATCAGGGAGGAACAAAGGGGTCCAAAAAGGTGGTCCAGCAACCTTCAAGATGTCAGGTTGCCATATTTGCAGTTTTAGCAATGGTGGTGCGAAGAAAACCTGATGTATTGATTAGTCTATTTCCTGTGATGAGGGAAAGTTCGAAATATCAAGGCCAAGATAAGCTTCCGCTGACAGTATGGTTGATTACGCAGGCCTCTCAAGGAGATCTGGTTGCTGGGCTGTACACGTGGGTACATCTTCTCCTGCCTCTATTGTGTAGTAAATCAGGGTGTAATCCACAGTCCAGAGACTTGATTTTGCAGTCAGTGGAAAGAATTTTATCTTCTCCCAAAGCTCGCCCTATTCTATTAAACGGTGCTGTCAGGAAAGGGGAGCGCATAGTGCCACCATCAGCTCTTGATCTCTTGATGAGAGTTTCTTTTCCTTCACCTTCAGCACGAGATAAGGCAACTGAGAGATTTGAAGCTGTCTACCCAACCTTAAAAGAGATTTCCCTTGCTGGATCCCCAGGAAGCAAAACGATGAGGCAGGTTGCACAGAAGATACTGAATTATTCAGTTAAAGCTGTTAAAAAAGGCATTCCTGGTCTCTCTAAGGAAGCAAGCTCTATATTTATCTGGTGTTTGACACATTGTCCCAAATGTTACAAGCAATGGGACATGCTTTATCTGGACAATCTTGATGCAAGTGTTATTGTGCTGAAAAAGCTATCTGATGAGTGGAAGGAGCATTCCGTTAAGCATACTTCTCTTGACCTCTTGAGGGAAACAATCAAGAGTTTCAGGGAGAAGAATGAGAAAGCGTTGGCCAATGGAGATGTTTTGGTGCACCATTCGTTGCTGAAGGATGCAGATAAATATTGCAAACTGATTTTAGGACAGTCATCCCAAGGCCATGGATGCATGAAAAGCATGGTTCTGGTGTCTGTTGCCCTTGCTATGGGTGCTGCTATCATGTCCCAAAACATACAACCCCAAGACCTCAAGAAACTTGCAGCCATGTTTAACTTCCCACTGACTATAAATGGAGGAATTTCGCAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

579

Amino Acids

64.14

Weight (kDa)

9.18

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 110 - 553 3e-19 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 1210
AccB1I GGYRCC 1 cut(s) 302
AccBSI CCGCTC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 1161
AccII CGCG 1 cut(s) 308
AclWI GGATC 4 cut(s) 553, 617, 1188, 1201
AcoI YGGCCR 5 cut(s) 81, 360, 475, 613, 1497
AcsI RAATTY 3 cut(s) 10, 1005, 1722
AcuI CTGAAG 3 cut(s) 648, 1107, 1553
AcvI CACGTG 1 cut(s) 918
AcyI GRCGYC 2 cut(s) 207, 303
AfaI GTAC 2 cut(s) 914, 924
AfeI AGCGCT 1 cut(s) 620
AfiI CCNNNNNNNGG 2 cut(s) 1453, 1630
AflIII ACRYGT 1 cut(s) 915
AgeI ACCGGT 1 cut(s) 94
AgsI TTSAA 6 cut(s) 340, 520, 529, 684, 735, 1154
AhdI GACNNNNNGTC 1 cut(s) 1323
AjnI CCWGG 4 cut(s) 119, 512, 1198, 1273
AjuI GAANNNNNNNTTGG 2 cut(s) 837, 869
Alw21I GWGCWC 1 cut(s) 1521
Alw26I GTCTC 4 cut(s) 547, 660, 972, 1283
Alw44I GTGCAC 1 cut(s) 1517
AlwI GGATC 4 cut(s) 553, 617, 1188, 1201
Aor51HI AGCGCT 1 cut(s) 620
AoxI GGCC 9 cut(s) 51, 81, 360, 475, 613, 841, 882, 1497, 1582
ApaLI GTGCAC 1 cut(s) 1517
ApeKI GCWGC 4 cut(s) 226, 436, 1637, 1685
ApoI RAATTY 3 cut(s) 10, 1005, 1722
ArsI GACNNNNNNTTYG 2 cut(s) 705, 737
AsiGI ACCGGT 1 cut(s) 94
Asp700I GAANNNNTTC 1 cut(s) 30
AspLEI GCGC 5 cut(s) 305, 371, 502, 621, 1068
AspS9I GGNCC 5 cut(s) 97, 238, 639, 708, 721
AsuHPI GGTGA 1 cut(s) 1110
AsuII TTCGAA 1 cut(s) 830
AvaII GGWCC 5 cut(s) 97, 238, 639, 708, 721
AxyI CCTNAGG 1 cut(s) 634
BaeGI GKGCMC 1 cut(s) 1521
BalI TGGCCA 2 cut(s) 83, 1499
BamHI GGATCC 1 cut(s) 1193
BanI GGYRCC 1 cut(s) 302
BauI CACGAG 1 cut(s) 1128
BbrPI CACGTG 1 cut(s) 918
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 1521
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 4 cut(s) 213, 448, 1624, 1697
BccI CCATC 3 cut(s) 73, 544, 1087
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 4 cut(s) 121, 514, 1200, 1275
BcoDI GTCTC 4 cut(s) 547, 660, 972, 1283
BfaI CTAG 1 cut(s) 659
BfoI RGCGCY 3 cut(s) 306, 372, 622
BfuAI ACCTGC 1 cut(s) 1210
BglII AGATCT 1 cut(s) 895
BisI GCNGC 5 cut(s) 227, 306, 437, 1638, 1686
BlsI GCNGC 5 cut(s) 228, 307, 438, 1639, 1687
Bme1390I CCNGG 4 cut(s) 121, 514, 1200, 1275
Bme18I GGWCC 5 cut(s) 97, 238, 639, 708, 721
BmeRI GACNNNNNGTC 1 cut(s) 1323
BmgT120I GGNCC 5 cut(s) 97, 238, 639, 708, 721
BmiI GGNNCC 4 cut(s) 304, 640, 709, 1195
BmrFI CCNGG 4 cut(s) 121, 514, 1200, 1275
BmsI GCATC 4 cut(s) 277, 1363, 1528, 1580
BpiI GAAGAC 1 cut(s) 202
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 830
BpuEI CTTGAG 3 cut(s) 873, 1471, 1658
Bsa29I ATCGAT 1 cut(s) 29
BsaAI YACGTR 1 cut(s) 918
BsaHI GRCGYC 2 cut(s) 207, 303
BsaI GGTCTC 2 cut(s) 660, 1283
BsaJI CCNNGG 8 cut(s) 54, 234, 306, 363, 512, 1198, 1579, 1585
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 885, 915, 915, 945
Bsc4I CCNNNNNNNGG 2 cut(s) 1453, 1630
Bse118I RCCGGY 2 cut(s) 94, 299
Bse1I ACTGG 1 cut(s) 611
Bse21I CCTNAGG 1 cut(s) 634
Bse3DI GCAATG 2 cut(s) 774, 1349
BseBI CCWGG 4 cut(s) 121, 514, 1200, 1275
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 8 cut(s) 54, 234, 306, 363, 512, 1198, 1579, 1585
BseGI GGATG 4 cut(s) 10, 1543, 1574, 1595
BseLI CCNNNNNNNGG 2 cut(s) 1453, 1630
BseMI GCAATG 2 cut(s) 774, 1349
BseMII CTCAG 4 cut(s) 26, 282, 518, 1134
BseNI ACTGG 1 cut(s) 611
BseRI GAGGAG 1 cut(s) 262
BseSI GKGCMC 1 cut(s) 1521
BseX3I CGGCCG 1 cut(s) 475
BseXI GCAGC 4 cut(s) 213, 448, 1624, 1697
BseYI CCCAGC 1 cut(s) 905
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 9 cut(s) 53, 83, 362, 477, 615, 843, 884, 1499, 1584
BshNI GGYRCC 1 cut(s) 302
BshTI ACCGGT 1 cut(s) 94
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 1521
BsiSI CCGG 4 cut(s) 95, 176, 244, 300
BslFI GGGAC 3 cut(s) 1311, 1361, 1633
BslI CCNNNNNNNGG 2 cut(s) 1453, 1630
BsmAI GTCTC 4 cut(s) 547, 660, 972, 1283
BsmFI GGGAC 3 cut(s) 1311, 1361, 1633
BsmI GAATGC 4 cut(s) 229, 666, 1269, 1420
BsnI GGCC 9 cut(s) 53, 83, 362, 477, 615, 843, 884, 1499, 1584
Bso31I GGTCTC 2 cut(s) 660, 1283
Bsp119I TTCGAA 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 1521
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
Bsp19I CCATGG 1 cut(s) 1585
BspANI GGCC 9 cut(s) 53, 83, 362, 477, 615, 843, 884, 1499, 1584
BspCNI CTCAG 4 cut(s) 27, 283, 517, 1135
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 4 cut(s) 304, 640, 709, 1195
BspMI ACCTGC 1 cut(s) 1210
BspPI GGATC 4 cut(s) 553, 617, 1188, 1201
BspT104I TTCGAA 1 cut(s) 830
BspT107I GGYRCC 1 cut(s) 302
BspTNI GGTCTC 2 cut(s) 660, 1283
BsrBI CCGCTC 1 cut(s) 216
BsrDI GCAATG 2 cut(s) 774, 1349
BsrFI RCCGGY 2 cut(s) 94, 299
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 1 cut(s) 611
BssAI RCCGGY 2 cut(s) 94, 299
BssECI CCNNGG 8 cut(s) 54, 234, 306, 363, 512, 1198, 1579, 1585
BssMI GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
BssNI GRCGYC 2 cut(s) 207, 303
BssSI CACGAG 1 cut(s) 1128
BssT1I CCWWGG 2 cut(s) 1579, 1585
Bst2BI CACGAG 1 cut(s) 1128
Bst2UI CCWGG 4 cut(s) 121, 514, 1200, 1275
Bst4CI ACNGT 8 cut(s) 130, 281, 358, 541, 865, 972, 1046, 1572
BstACI GRCGYC 2 cut(s) 207, 303
BstAUI TGTACA 1 cut(s) 912
BstBAI YACGTR 1 cut(s) 918
BstBI TTCGAA 1 cut(s) 830
BstC8I GCNNGC 7 cut(s) 85, 301, 320, 617, 882, 1027, 1294
BstDEI CTNAG 6 cut(s) 35, 291, 504, 634, 1143, 1284
BstDSI CCRYGG 2 cut(s) 306, 1585
BstF5I GGATG 4 cut(s) 10, 1543, 1574, 1595
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 3 cut(s) 306, 372, 622
BstHHI GCGC 5 cut(s) 305, 371, 502, 621, 1068
BstKTI GATC 8 cut(s) 24, 193, 381, 561, 612, 898, 1093, 1196
BstMAI GTCTC 4 cut(s) 547, 660, 972, 1283
BstMBI GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
BstMCI CGRYCG 1 cut(s) 478
BstNI CCWGG 4 cut(s) 121, 514, 1200, 1275
BstNSI RCATGY 1 cut(s) 1354
BstSCI CCNGG 4 cut(s) 119, 512, 1198, 1273
BstSLI GKGCMC 1 cut(s) 1521
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 4 cut(s) 213, 448, 1624, 1697
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 3 cut(s) 558, 895, 1193
BstXI CCANNNNNNTGG 2 cut(s) 718, 1586
BstYI RGATCY 3 cut(s) 558, 895, 1193
BstZI CGGCCG 1 cut(s) 475
Bsu15I ATCGAT 1 cut(s) 29
Bsu36I CCTNAGG 1 cut(s) 634
BsuRI GGCC 9 cut(s) 53, 83, 362, 477, 615, 843, 884, 1499, 1584
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 2 cut(s) 306, 1585
BtgZI GCGATG 2 cut(s) 288, 300
BtsCI GGATG 4 cut(s) 10, 1543, 1574, 1595
BtsIMutI CAGTG 4 cut(s) 537, 624, 1002, 1703
BveI ACCTGC 1 cut(s) 1210
Cac8I GCNNGC 7 cut(s) 85, 301, 320, 617, 882, 1027, 1294
CfoI GCGC 5 cut(s) 305, 371, 502, 621, 1068
Cfr10I RCCGGY 2 cut(s) 94, 299
Cfr13I GGNCC 5 cut(s) 97, 238, 639, 708, 721
Cfr42I CCGCGG 1 cut(s) 309
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 119, 446
Csp6I GTAC 2 cut(s) 913, 923
CspAI ACCGGT 1 cut(s) 94
CviAII CATG 6 cut(s) 1351, 1586, 1594, 1603, 1645, 1690
CviQI GTAC 2 cut(s) 913, 923
DdeI CTNAG 6 cut(s) 35, 291, 504, 634, 1143, 1284
DinI GGCGCC 1 cut(s) 304
DpnI GATC 8 cut(s) 23, 192, 380, 560, 611, 897, 1092, 1195
DpnII GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
DriI GACNNNNNGTC 1 cut(s) 1323
EaeI YGGCCR 5 cut(s) 81, 360, 475, 613, 1497
EagI CGGCCG 1 cut(s) 475
Eam1105I GACNNNNNGTC 1 cut(s) 1323
EciI GGCGGA 2 cut(s) 74, 162
EclXI CGGCCG 1 cut(s) 475
Eco130I CCWWGG 2 cut(s) 1579, 1585
Eco147I AGGCCT 1 cut(s) 884
Eco31I GGTCTC 2 cut(s) 660, 1283
Eco47I GGWCC 5 cut(s) 97, 238, 639, 708, 721
Eco47III AGCGCT 1 cut(s) 620
Eco52I CGGCCG 1 cut(s) 475
Eco57I CTGAAG 3 cut(s) 648, 1107, 1553
Eco72I CACGTG 1 cut(s) 918
Eco81I CCTNAGG 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 4 cut(s) 119, 512, 1198, 1273
EcoT14I CCWWGG 2 cut(s) 1579, 1585
EcoT22I ATGCAT 1 cut(s) 1595
EgeI GGCGCC 1 cut(s) 304
EheI GGCGCC 1 cut(s) 304
ErhI CCWWGG 2 cut(s) 1579, 1585
FaeI CATG 6 cut(s) 1354, 1589, 1597, 1606, 1648, 1693
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 3 cut(s) 1311, 1361, 1633
FatI CATG 6 cut(s) 1350, 1585, 1593, 1602, 1644, 1689
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 1161
Fnu4HI GCNGC 5 cut(s) 227, 306, 437, 1638, 1686
FokI GGATG 4 cut(s) 17, 1550, 1561, 1602
Fsp4HI GCNGC 5 cut(s) 227, 306, 437, 1638, 1686
FspBI CTAG 1 cut(s) 659
GlaI GCGC 5 cut(s) 304, 370, 501, 620, 1067
GluI GCNGC 5 cut(s) 227, 306, 437, 1638, 1686
GsaI CCCAGC 1 cut(s) 909
HaeII RGCGCY 3 cut(s) 306, 372, 622
HaeIII GGCC 9 cut(s) 53, 83, 362, 477, 615, 843, 884, 1499, 1584
HapII CCGG 4 cut(s) 95, 176, 244, 300
HgaI GACGC 2 cut(s) 119, 446
HhaI GCGC 5 cut(s) 305, 371, 502, 621, 1068
Hin1I GRCGYC 2 cut(s) 207, 303
Hin1II CATG 6 cut(s) 1354, 1589, 1597, 1606, 1648, 1693
Hin6I GCGC 5 cut(s) 303, 369, 500, 619, 1066
HinP1I GCGC 5 cut(s) 303, 369, 500, 619, 1066
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 6 cut(s) 26, 31, 179, 252, 383, 533
HpaII CCGG 4 cut(s) 95, 176, 244, 300
HphI GGTGA 1 cut(s) 1110
Hpy166II GTNNAC 5 cut(s) 537, 604, 915, 1162, 1519
Hpy188I TCNGA 6 cut(s) 21, 36, 235, 257, 580, 1405
Hpy8I GTNNAC 5 cut(s) 537, 604, 915, 1162, 1519
Hpy99I CGWCG 3 cut(s) 113, 156, 209
HpyAV CCTTC 8 cut(s) 193, 322, 652, 741, 1125, 1131, 1408, 1528
HpyCH4III ACNGT 8 cut(s) 130, 281, 358, 541, 865, 972, 1046, 1572
HpyCH4IV ACGT 2 cut(s) 207, 917
HpyF3I CTNAG 6 cut(s) 35, 291, 504, 634, 1143, 1284
HpySE526I ACGT 2 cut(s) 207, 917
Hsp92I GRCGYC 2 cut(s) 207, 303
Hsp92II CATG 6 cut(s) 1354, 1589, 1597, 1606, 1648, 1693
HspAI GCGC 5 cut(s) 303, 369, 500, 619, 1066
KasI GGCGCC 1 cut(s) 302
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
KspI CCGCGG 1 cut(s) 309
Kzo9I GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
LmnI GCTCC 4 cut(s) 221, 626, 1063, 1417
Lsp1109I GCAGC 4 cut(s) 213, 448, 1624, 1697
LweI GCATC 4 cut(s) 277, 1363, 1528, 1580
MaeI CTAG 1 cut(s) 659
MaeII ACGT 2 cut(s) 207, 917
MaeIII GTNAC 1 cut(s) 1334
MalI GATC 8 cut(s) 23, 192, 380, 560, 611, 897, 1092, 1195
MbiI CCGCTC 1 cut(s) 216
MboI GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
MflI RGATCY 3 cut(s) 558, 895, 1193
MhlI GDGCHC 1 cut(s) 1521
MlsI TGGCCA 2 cut(s) 83, 1499
MluCI AATT 6 cut(s) 10, 168, 335, 1005, 1240, 1722
MluNI TGGCCA 2 cut(s) 83, 1499
Mly113I GGCGCC 1 cut(s) 303
MlyI GAGTC 2 cut(s) 377, 542
Mox20I TGGCCA 2 cut(s) 83, 1499
Mph1103I ATGCAT 1 cut(s) 1595
MreI CGCCGGCG 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 299
MroXI GAANNNNTTC 1 cut(s) 30
MscI TGGCCA 2 cut(s) 83, 1499
MseI TTAA 7 cut(s) 72, 1040, 1172, 1251, 1260, 1428, 1695
MslI CAYNNNNRTG 1 cut(s) 396
Msp20I TGGCCA 2 cut(s) 83, 1499
MspA1I CMGCKG 2 cut(s) 308, 859
MspI CCGG 4 cut(s) 95, 176, 244, 300
MspR9I CCNGG 4 cut(s) 121, 514, 1200, 1275
Mva1269I GAATGC 4 cut(s) 229, 666, 1269, 1420
MvaI CCWGG 4 cut(s) 121, 514, 1200, 1275
MvnI CGCG 1 cut(s) 308
NaeI GCCGGC 1 cut(s) 301
NarI GGCGCC 1 cut(s) 303
NcoI CCATGG 1 cut(s) 1585
NdeII GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 6 cut(s) 1354, 1589, 1597, 1606, 1648, 1693
NlaIV GGNNCC 4 cut(s) 304, 640, 709, 1195
NmeAIII GCCGAG 5 cut(s) 79, 365, 388, 436, 503
NsiI ATGCAT 1 cut(s) 1595
NspI RCATGY 1 cut(s) 1354
NspV TTCGAA 1 cut(s) 830
PceI AGGCCT 1 cut(s) 884
PctI GAATGC 4 cut(s) 229, 666, 1269, 1420
PdiI GCCGGC 1 cut(s) 301
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 4 cut(s) 26, 31, 179, 252
PinAI ACCGGT 1 cut(s) 94
PkrI GCNGC 5 cut(s) 228, 307, 438, 1639, 1687
PleI GAGTC 2 cut(s) 377, 541
PluTI GGCGCC 1 cut(s) 306
PmaCI CACGTG 1 cut(s) 918
PmlI CACGTG 1 cut(s) 918
PpsI GAGTC 2 cut(s) 377, 541
Ppu21I YACGTR 1 cut(s) 918
PpuMI RGGWCCY 1 cut(s) 639
PsiI TTATAA 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 639
Psp6I CCWGG 4 cut(s) 119, 512, 1198, 1273
PspCI CACGTG 1 cut(s) 918
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 4 cut(s) 119, 512, 1198, 1273
PspN4I GGNNCC 4 cut(s) 304, 640, 709, 1195
PspPI GGNCC 5 cut(s) 97, 238, 639, 708, 721
PspPPI RGGWCCY 1 cut(s) 639
PsuI RGATCY 3 cut(s) 558, 895, 1193
RsaI GTAC 2 cut(s) 914, 924
RsaNI GTAC 2 cut(s) 913, 923
RseI CAYNNNNRTG 1 cut(s) 396
SacII CCGCGG 1 cut(s) 309
SaqAI TTAA 7 cut(s) 72, 1040, 1172, 1251, 1260, 1428, 1695
SatI GCNGC 5 cut(s) 227, 306, 437, 1638, 1686
Sau3AI GATC 8 cut(s) 21, 190, 378, 558, 609, 895, 1090, 1193
Sau96I GGNCC 5 cut(s) 97, 238, 639, 708, 721
SchI GAGTC 2 cut(s) 377, 542
ScrFI CCNGG 4 cut(s) 121, 514, 1200, 1275
SduI GDGCHC 1 cut(s) 1521
SfaNI GCATC 4 cut(s) 277, 1363, 1528, 1580
SfoI GGCGCC 1 cut(s) 304
Sfr303I CCGCGG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 830
SgrAI CRCCGGYG 1 cut(s) 299
SgrBI CCGCGG 1 cut(s) 309
SinI GGWCC 5 cut(s) 97, 238, 639, 708, 721
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 888, 1450, 1673
SmoI CTYRAG 3 cut(s) 888, 1450, 1673
Sse9I AATT 6 cut(s) 10, 168, 335, 1005, 1240, 1722
SseBI AGGCCT 1 cut(s) 884
SspDI GGCGCC 1 cut(s) 302
SspI AATATT 1 cut(s) 1550
SspMI CTAG 1 cut(s) 659
StuI AGGCCT 1 cut(s) 884
StyD4I CCNGG 4 cut(s) 119, 512, 1198, 1273
StyI CCWWGG 2 cut(s) 1579, 1585
TaaI ACNGT 8 cut(s) 130, 281, 358, 541, 865, 972, 1046, 1572
TaiI ACGT 2 cut(s) 210, 920
TaqI TCGA 4 cut(s) 24, 29, 381, 830
TasI AATT 6 cut(s) 10, 168, 335, 1005, 1240, 1722
TatI WGTACW 1 cut(s) 912
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 4 cut(s) 26, 31, 179, 252
Tru1I TTAA 7 cut(s) 72, 1040, 1172, 1251, 1260, 1428, 1695
Tru9I TTAA 7 cut(s) 72, 1040, 1172, 1251, 1260, 1428, 1695
TscAI CASTG 4 cut(s) 544, 631, 1002, 1710
TseI GCWGC 4 cut(s) 226, 436, 1637, 1685
TspDTI ATGAA 1 cut(s) 1610
TspGWI ACGGA 3 cut(s) 434, 481, 1414
TspRI CASTG 4 cut(s) 544, 631, 1002, 1710
VneI GTGCAC 1 cut(s) 1517
VpaK11BI GGWCC 5 cut(s) 97, 238, 639, 708, 721
XapI RAATTY 3 cut(s) 10, 1005, 1722
XbaI TCTAGA 1 cut(s) 658
XceI RCATGY 1 cut(s) 1354
XmiI GTMKAC 1 cut(s) 1161
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 1 cut(s) 659
ZraI GACGTC 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 1595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.