Rroxscaffold_6G00391870

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002090
Physical Location & Seq
Reverse (-)
10714378 .. 10718792
4415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00391870.1

Sequence Viewer

Length: 1359 bp
ATGGACGAGAATTCAGCACTGATCGAATCGATTCTGAGAGAGGAAGACGAGGCGGAGGCTGACAAATATGTAAAGAAGATGAGCAGCACTGCGCCAGTCCAAAACGGCGACGCTTACGCCTGGCAAACTGTTTCTTATCACAAGCGAAACCGACGACTTTCTCAGCCAATTCCGCCTGATTCTGTCCGCGATCCCTCCAACGGCGACCCGCATTCCGACGACGATAGCGATGCCGAGGTCGCCGCTGCCGCGGAAAATGGCGAGCCGAAGAAGGCCAAAGTGAAGAAGCCGAAGAAGCCCAAGCTGACTGTGGCCGAGGCCGCTTCCAAGATCGACGCGGCTCATCTCGGTGCTTTTCTCGCCGAGATAACGATCCCTCTTTCTCATATATCTGAAAATGTTTATAAGACATCAGTTGACTGGATCGGCCAGCGCTCCACTGAAGCCCTTGGCTCCTTCGTGTTGTGGTCACTAGATTGCATTCTTGCTGACCTTGCAAGTCATCATGGAGCAGCAAAGGGATCCAAAAAGGTGGTCCGGCCACCTTCAAGATGTCAGGTTGCCATATTCGCTGTTTTAGCAATGGTGTTGCGAAAAAAACCTGATGCCGCTCAAGGAGATCTGGTTGTTGGGTTGTACACATGGGTACATCTTCTCCTGCCTATATTGAGTAGCAAGTCAGGTTGTAATCCACAGTCTAGAGACTTGATTTTGCAGTCAGTGGAAAGAATTTTATCGTCTCCAAAAGCTCGGCCTATTCTATTAAATGGTGCTGTTAGGAAAGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGCAACTGAGAGATTTGAAGCTGTCTATCCAACCTTGAAAGAGATTGCCCTTGCTGGATCCCCAGGAAGCAAAACAGTGAGGCAGGTTGCACAGAAGACACTGAATTATTCAGTTAAAGCTGTTAAAGAAGGCAACCCTGATCTCTCTAAGGAGGCAACCGCTATATTTATCTGGTGTTTGACACAAAGTCCCGAATGTTACAAGCAATGGGACATGCTTTATCTGGACAATCTTGATGCAAGTGTTATTGTGCTGAAAAAGTTATCTGACGAGTGGAAGGAGCACTCCAATGAAAAAACATTGGCAAAAGGAGATGATTTGGTGCGCCATTCGTTGTTGAAGGATGCAGATAAATATTGCAAGCTGATCTTAGGACGGTCATCTCAAGGCCGTGGATGCATGAAAAGCATGGTTCTGGTGTCTGTTGCCCTTGCTATGGGTGCTGCTATCATGTCCCAAAACATACAACCCGAAGACCTCAAGAAACTTGCAGCCACGTTTAACTTCCCCCCTGACTCTCTAAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

452

Amino Acids

49.4

Weight (kDa)

8.48

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 205 - 373 4.5e-08 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc36I ACCTGC 1 cut(s) 901
AccBSI CCGCTC 1 cut(s) 611
AccII CGCG 3 cut(s) 189, 251, 338
AclWI GGATC 7 cut(s) 185, 367, 431, 516, 529, 879, 892
AcoI YGGCCR 3 cut(s) 312, 427, 539
AcsI RAATTY 2 cut(s) 10, 729
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 796
AfaI GTAC 2 cut(s) 638, 648
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 200, 1264
AgsI TTSAA 4 cut(s) 549, 845, 865, 1168
AhdI GACNNNNNGTC 1 cut(s) 1014
AjnI CCWGG 2 cut(s) 119, 889
AluBI AGCT 5 cut(s) 304, 749, 848, 947, 1192
AluI AGCT 5 cut(s) 304, 749, 848, 947, 1192
Alw21I GWGCWC 2 cut(s) 792, 1113
Alw26I GTCTC 2 cut(s) 696, 744
AlwI GGATC 7 cut(s) 185, 367, 431, 516, 529, 879, 892
Aor51HI AGCGCT 1 cut(s) 434
AoxI GGCC 7 cut(s) 273, 312, 318, 427, 539, 752, 1216
ApeKI GCWGC 5 cut(s) 84, 245, 512, 1271, 1319
ApoI RAATTY 2 cut(s) 10, 729
ArsI GACNNNNNNTTYG 2 cut(s) 519, 551
Asp700I GAANNNNTTC 1 cut(s) 30
AspLEI GCGC 3 cut(s) 94, 435, 1155
AspS9I GGNCC 1 cut(s) 535
AvaII GGWCC 1 cut(s) 535
BamHI GGATCC 2 cut(s) 521, 884
BbsI GAAGAC 3 cut(s) 51, 929, 1308
Bbv12I GWGCWC 2 cut(s) 792, 1113
BbvI GCAGC 5 cut(s) 96, 232, 524, 1258, 1331
BccI CCATC 1 cut(s) 811
BceAI ACGGC 3 cut(s) 121, 217, 1203
BcgI CGANNNNNNTGC 2 cut(s) 212, 246
BciT130I CCWGG 2 cut(s) 121, 891
BcoDI GTCTC 2 cut(s) 696, 744
BfaI CTAG 2 cut(s) 473, 699
BfoI RGCGCY 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 901
BglII AGATCT 1 cut(s) 619
Bme1390I CCNGG 2 cut(s) 121, 891
Bme18I GGWCC 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 1014
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 3 cut(s) 454, 523, 886
BmrFI CCNGG 2 cut(s) 121, 891
BmsI GCATC 5 cut(s) 220, 595, 1054, 1162, 1214
BpiI GAAGAC 3 cut(s) 51, 929, 1308
BpuEI CTTGAG 3 cut(s) 597, 1197, 1292
Bsa29I ATCGAT 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 371
BsaJI CCNNGG 6 cut(s) 234, 249, 315, 448, 889, 1219
BsaXI ACNNNNNCTCC 4 cut(s) 609, 639, 639, 669
Bsc4I CCNNNNNNNGG 2 cut(s) 200, 1264
Bse1I ACTGG 2 cut(s) 95, 425
Bse3DI GCAATG 2 cut(s) 588, 1040
Bse8I GATNNNNATC 1 cut(s) 371
BseBI CCWGG 2 cut(s) 121, 891
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 6 cut(s) 234, 249, 315, 448, 889, 1219
BseGI GGATG 2 cut(s) 1177, 1229
BseJI GATNNNNATC 1 cut(s) 371
BseLI CCNNNNNNNGG 2 cut(s) 200, 1264
BseMI GCAATG 2 cut(s) 588, 1040
BseMII CTCAG 3 cut(s) 26, 176, 825
BseNI ACTGG 2 cut(s) 95, 425
BseXI GCAGC 5 cut(s) 96, 232, 524, 1258, 1331
Bsh1236I CGCG 3 cut(s) 189, 251, 338
BshFI GGCC 7 cut(s) 275, 314, 320, 429, 541, 754, 1218
BshVI ATCGAT 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 792, 1113
BsiSI CCGG 1 cut(s) 538
BslFI GGGAC 3 cut(s) 1002, 1052, 1267
BslI CCNNNNNNNGG 2 cut(s) 200, 1264
BsmAI GTCTC 2 cut(s) 696, 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 3 cut(s) 1002, 1052, 1267
BsmI GAATGC 2 cut(s) 211, 480
BsnI GGCC 7 cut(s) 275, 314, 320, 429, 541, 754, 1218
Bsp1286I GDGCHC 2 cut(s) 792, 1113
Bsp1407I TGTACA 1 cut(s) 636
BspANI GGCC 7 cut(s) 275, 314, 320, 429, 541, 754, 1218
BspCNI CTCAG 3 cut(s) 27, 175, 826
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 3 cut(s) 189, 251, 338
BspLI GGNNCC 3 cut(s) 454, 523, 886
BspMI ACCTGC 1 cut(s) 901
BspPI GGATC 7 cut(s) 185, 367, 431, 516, 529, 879, 892
BsrBI CCGCTC 1 cut(s) 611
BsrDI GCAATG 2 cut(s) 588, 1040
BsrGI TGTACA 1 cut(s) 636
BsrI ACTGG 2 cut(s) 95, 425
BssECI CCNNGG 6 cut(s) 234, 249, 315, 448, 889, 1219
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 2 cut(s) 121, 891
Bst4CI ACNGT 5 cut(s) 130, 310, 696, 904, 1206
BstAPI GCANNNNNTGC 1 cut(s) 796
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 3 cut(s) 263, 431, 1190
BstDEI CTNAG 5 cut(s) 35, 162, 834, 975, 1198
BstDSI CCRYGG 2 cut(s) 249, 1219
BstF5I GGATG 2 cut(s) 1177, 1229
BstFNI CGCG 3 cut(s) 189, 251, 338
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 3 cut(s) 94, 435, 1155
BstMAI GTCTC 2 cut(s) 696, 744
BstNI CCWGG 2 cut(s) 121, 891
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 2 cut(s) 119, 889
BstUI CGCG 3 cut(s) 189, 251, 338
BstV1I GCAGC 5 cut(s) 96, 232, 524, 1258, 1331
BstV2I GAAGAC 3 cut(s) 51, 929, 1308
BstX2I RGATCY 3 cut(s) 521, 619, 884
BstXI CCANNNNNNTGG 1 cut(s) 532
BstYI RGATCY 3 cut(s) 521, 619, 884
Bsu15I ATCGAT 1 cut(s) 29
BsuRI GGCC 7 cut(s) 275, 314, 320, 429, 541, 754, 1218
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 2 cut(s) 249, 1219
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 2 cut(s) 1177, 1229
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 6 cut(s) 17, 87, 438, 726, 909, 926
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 3 cut(s) 263, 431, 1190
CfoI GCGC 3 cut(s) 94, 435, 1155
Cfr13I GGNCC 1 cut(s) 535
Cfr42I CCGCGG 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 119, 344
Csp6I GTAC 2 cut(s) 637, 647
CviAII CATG 6 cut(s) 506, 642, 1042, 1228, 1237, 1279
CviQI GTAC 2 cut(s) 637, 647
DdeI CTNAG 5 cut(s) 35, 162, 834, 975, 1198
DraIII CACNNNGTG 1 cut(s) 796
DriI GACNNNNNGTC 1 cut(s) 1014
EaeI YGGCCR 3 cut(s) 312, 427, 539
Eam1105I GACNNNNNGTC 1 cut(s) 1014
EciI GGCGGA 2 cut(s) 68, 162
Eco130I CCWWGG 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 535
Eco47III AGCGCT 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 462
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 119, 889
EcoT14I CCWWGG 1 cut(s) 448
EcoT22I ATGCAT 1 cut(s) 1229
ErhI CCWWGG 1 cut(s) 448
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 6 cut(s) 509, 645, 1045, 1231, 1240, 1282
FalI AAGNNNNNCTT 2 cut(s) 1181, 1213
FaqI GGGAC 3 cut(s) 1002, 1052, 1267
FatI CATG 6 cut(s) 505, 641, 1041, 1227, 1236, 1278
FauI CCCGC 1 cut(s) 216
FokI GGATG 2 cut(s) 1184, 1236
FspBI CTAG 2 cut(s) 473, 699
GlaI GCGC 3 cut(s) 93, 434, 1154
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 7 cut(s) 275, 314, 320, 429, 541, 754, 1218
HapII CCGG 1 cut(s) 538
HgaI GACGC 2 cut(s) 119, 344
HhaI GCGC 3 cut(s) 94, 435, 1155
Hin1II CATG 6 cut(s) 509, 645, 1045, 1231, 1240, 1282
Hin6I GCGC 3 cut(s) 92, 433, 1153
HinP1I GCGC 3 cut(s) 92, 433, 1153
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 4 cut(s) 26, 31, 179, 1343
HpaII CCGG 1 cut(s) 538
Hpy166II GTNNAC 2 cut(s) 418, 639
Hpy188I TCNGA 4 cut(s) 36, 217, 394, 1096
Hpy188III TCNNGA 7 cut(s) 549, 699, 820, 1019, 1052, 1061, 1309
Hpy8I GTNNAC 2 cut(s) 418, 639
Hpy99I CGWCG 5 cut(s) 113, 156, 221, 224, 338
HpyAV CCTTC 6 cut(s) 265, 466, 555, 950, 1099, 1162
HpyCH4III ACNGT 5 cut(s) 130, 310, 696, 904, 1206
HpyCH4IV ACGT 1 cut(s) 1325
HpyCH4V TGCA 9 cut(s) 480, 497, 715, 917, 1067, 1175, 1188, 1227, 1319
HpyF3I CTNAG 5 cut(s) 35, 162, 834, 975, 1198
HpySE526I ACGT 1 cut(s) 1325
Hsp92II CATG 6 cut(s) 509, 645, 1045, 1231, 1240, 1282
HspAI GCGC 3 cut(s) 92, 433, 1153
KspI CCGCGG 1 cut(s) 252
LmnI GCTCC 5 cut(s) 440, 458, 509, 787, 1108
Lsp1109I GCAGC 5 cut(s) 96, 232, 524, 1258, 1331
LweI GCATC 5 cut(s) 220, 595, 1054, 1162, 1214
MaeI CTAG 2 cut(s) 473, 699
MaeII ACGT 1 cut(s) 1325
MaeIII GTNAC 2 cut(s) 468, 1025
MbiI CCGCTC 1 cut(s) 611
MboII GAAGA 8 cut(s) 56, 88, 280, 295, 304, 644, 934, 1313
MflI RGATCY 3 cut(s) 521, 619, 884
MhlI GDGCHC 2 cut(s) 792, 1113
MluCI AATT 4 cut(s) 10, 168, 729, 931
MlyI GAGTC 1 cut(s) 1337
MmeI TCCRAC 3 cut(s) 222, 240, 881
Mph1103I ATGCAT 1 cut(s) 1229
MroXI GAANNNNTTC 1 cut(s) 30
MseI TTAA 4 cut(s) 764, 942, 951, 1329
MslI CAYNNNNRTG 2 cut(s) 348, 1116
MspA1I CMGCKG 2 cut(s) 245, 251
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 2 cut(s) 121, 891
Mva1269I GAATGC 2 cut(s) 211, 480
MvaI CCWGG 2 cut(s) 121, 891
MvnI CGCG 3 cut(s) 189, 251, 338
NlaIII CATG 6 cut(s) 509, 645, 1045, 1231, 1240, 1282
NlaIV GGNNCC 3 cut(s) 454, 523, 886
NmeAIII GCCGAG 4 cut(s) 259, 340, 388, 730
NmuCI GTSAC 1 cut(s) 468
NsiI ATGCAT 1 cut(s) 1229
NspI RCATGY 1 cut(s) 1045
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 2 cut(s) 211, 480
PdmI GAANNNNTTC 1 cut(s) 30
PfeI GAWTC 3 cut(s) 26, 31, 179
PleI GAGTC 1 cut(s) 1337
PpsI GAGTC 1 cut(s) 1337
PsiI TTATAA 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 119, 889
PspGI CCWGG 2 cut(s) 119, 889
PspN4I GGNNCC 3 cut(s) 454, 523, 886
PspPI GGNCC 1 cut(s) 535
PsuI RGATCY 3 cut(s) 521, 619, 884
RsaI GTAC 2 cut(s) 638, 648
RsaNI GTAC 2 cut(s) 637, 647
RseI CAYNNNNRTG 2 cut(s) 348, 1116
SacII CCGCGG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 764, 942, 951, 1329
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 1 cut(s) 1337
ScrFI CCNGG 2 cut(s) 121, 891
SduI GDGCHC 2 cut(s) 792, 1113
SfaNI GCATC 5 cut(s) 220, 595, 1054, 1162, 1214
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 1 cut(s) 535
SmiMI CAYNNNNRTG 2 cut(s) 348, 1116
SmlI CTYRAG 3 cut(s) 612, 1212, 1307
SmoI CTYRAG 3 cut(s) 612, 1212, 1307
Sse9I AATT 4 cut(s) 10, 168, 729, 931
SspI AATATT 1 cut(s) 1184
SspMI CTAG 2 cut(s) 473, 699
StyD4I CCNGG 2 cut(s) 119, 889
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 5 cut(s) 130, 310, 696, 904, 1206
TaiI ACGT 1 cut(s) 1328
TaqI TCGA 3 cut(s) 24, 29, 333
TasI AATT 4 cut(s) 10, 168, 729, 931
TatI WGTACW 1 cut(s) 636
TauI GCSGC 5 cut(s) 245, 251, 323, 341, 611
TfiI GAWTC 3 cut(s) 26, 31, 179
Tru1I TTAA 4 cut(s) 764, 942, 951, 1329
Tru9I TTAA 4 cut(s) 764, 942, 951, 1329
TscAI CASTG 6 cut(s) 24, 94, 445, 726, 909, 933
TseFI GTSAC 1 cut(s) 468
TseI GCWGC 5 cut(s) 84, 245, 512, 1271, 1319
Tsp45I GTSAC 1 cut(s) 468
TspDTI ATGAA 2 cut(s) 1134, 1244
TspRI CASTG 6 cut(s) 24, 94, 445, 726, 909, 933
VpaK11BI GGWCC 1 cut(s) 535
XapI RAATTY 2 cut(s) 10, 729
XbaI TCTAGA 1 cut(s) 698
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 30
XspI CTAG 2 cut(s) 473, 699
Zsp2I ATGCAT 1 cut(s) 1229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.