MD16G1023000.v1.1

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002090
Physical Location & Seq
Reverse (-)
1675846 .. 1680170
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1023000.v1.1.491

Sequence Viewer

Length: 1821 bp
ATGGAAGAAAATCACGCTGCAGCTTTCGAATCAATCGCCAACGGGGACCACCTCGACGCCGCATCCACCGCTGCAAACGACTACAGCAGTACCCATCACGCCCACGCCAACAACGACCACGGCTGGCAGAAGGTCACCGCGAAGCGCCCGAGGAAGAAGAAGGAATCGAACGCCGAAGTAATCAACAATCAGAACAAGCTCGTCCCCGGCATCGCGAGCGCCAACGGAAACGGCGTTTTCCGGTCGCTGGAGAAACAGTCGGAGGACCGGCGCCGGCGGATTCTCGAAGCTCAGAGGGCCAATGTCGACCTCGACGATGTTGCTCCGGCGAGATCGAAGCTCAGGTCCGACGACGAGGATGGGGATAATAGCGATGATGAAGCTGCGGCGCAGAACGTGAAGGCCGAGGAGGCGAAGAAATCCAAGCCCAAGAAGCCGAAGAAGCCTAAGGTCACGCTGGCCGAGGCGGCGGCTAAGATCGACGAGGCGAACGATCTCGCTGCATTCCTCATTGACATTTCGGCGTCGTACGAGTCGAAGGAGGACATACAGCTGATGCGTTTCGCGGACTATTTCGGCCGTGCATTCTCTGCGGTGTCTGCCTCTCAGTTTCCATGGGTGAAGATGTTCAAGGAGTCATCGGTGGCCAAGCTTGCCGATATCCCCCTTTCTCATATTTCTGAAGATGTTTACAAGACATCAGTTGACTGGATCAACCAGCGTTCCGTTGAGGCCCTTGGGTCTTTTATATTATGGTCCTTAGACAGCATTCTTGCAGACCTAGTGAGCCAAGTTTCCGGCACAAAGGGCAAGAAGGCTGTGCAGAATGTGTCTTCAAAATCTCAGGTTGCGATATTTGTGGTTGTAGCAATGGTATTGCGAAGAAAACCCGATGTCTTGATTAGTATATTGCCAACACTGAGAGAAAATTCAAAGTATCAAGGACAAGATAAGCTTCCAGTAATTATATGGATGATAGTTCAGGCTAGTCAAGGGGACCTCGCTGTTGGGTTGCATGCATGGGCCCGCAATGTGTTGCCTCTAGTCTGTGGAAAAAGTAGTAATCCACAGTCCCGGGATCTTGTTTTGCAGCTAGTGGAGAGAATTCTGTCTATGCCCAAGGCTCGTACTATTTTAGTAAATAGTGCTGTTAGGAAGGGGGAGCGTTTGGTGCCACCTTCTGCATTAGAGATACTTATGGGAGTTACCTTTCCCGGGCATTCAGCTAGAGTAAAGGCCACCGAAAGGTTCGAGGCTATATATCCAACCCTAAAAGCGGTCGCTCTAGCTGGCGCCCCTGGAAGCAAAGCAATGAAACAAGTTTCACAGCAGATAATGAGTTTTGCTGTCAAAGCTGCAGGAGAAAGTATACCTGAGCTATCCAATGAAGCAACTGGAATTTTCATTTGGTGTTTGACCCAACATGCTGATTGCTTCAAGCAGTGGGACAAGGTTTACGAGGAAAACTTAGAAGTGAGTGTCGCTATTCTGAAGAAACTTTCTAACCAATGGAAGGAACATTCTGCAAACCTCTCTCCTCTTGATCCTATGAGGGAATCTCTCAAAAGTTTTATGCGCAAGAATAAGAAGGCATTGGCTGGTGAGGCAGAGGATGTACACCGCGAAAAGCTTAGCAAGGAAGCAGACAAGTATTGTAAGACATTATTGGGAAAGCTATCACGGGGAATCGGGTGCAAGAAGACTCTGGCCCTTGCTGTTGTAGCCTTGGCTGTTGGTGCTGCTGTCATGTCCCCGAACATGGAATCTTGGGAGTTGGAGTTGAAGAAACTACCCGCGACTATCAGCTCTTTCTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

607

Amino Acids

66.55

Weight (kDa)

9.04

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 123 - 580 1.7e-20 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1577
AccB1I GGYRCC 3 cut(s) 270, 1171, 1292
AccI GTMKAC 2 cut(s) 306, 1369
AccII CGCG 5 cut(s) 140, 215, 566, 1623, 1796
AclWI GGATC 3 cut(s) 719, 1086, 1538
AcoI YGGCCR 3 cut(s) 459, 577, 645
AcsI RAATTY 3 cut(s) 928, 1104, 1398
AcuI CTGAAG 2 cut(s) 702, 1511
AcyI GRCGYC 4 cut(s) 57, 271, 524, 1293
AfaI GTAC 4 cut(s) 91, 530, 1129, 1617
AfiI CCNNNNNNNGG 6 cut(s) 247, 1215, 1245, 1276, 1513, 1759
AgsI TTSAA 6 cut(s) 631, 837, 933, 1438, 1783, 1817
AjnI CCWGG 1 cut(s) 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1390, 1422
AlwI GGATC 3 cut(s) 719, 1086, 1538
Ama87I CYCGRG 3 cut(s) 148, 1074, 1214
AoxI GGCC 9 cut(s) 297, 402, 459, 577, 645, 732, 1023, 1236, 1707
ApaI GGGCCC 1 cut(s) 1027
ApeKI GCWGC 8 cut(s) 17, 20, 71, 383, 500, 1090, 1355, 1739
ApoI RAATTY 3 cut(s) 928, 1104, 1398
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
Asp700I GAANNNNTTC 1 cut(s) 626
AspLEI GCGC 6 cut(s) 147, 221, 273, 391, 1295, 1578
AsuC2I CCSGG 5 cut(s) 207, 1075, 1076, 1215, 1216
AsuHPI GGTGA 3 cut(s) 127, 631, 1613
AsuII TTCGAA 1 cut(s) 27
AvaI CYCGRG 3 cut(s) 148, 1074, 1214
AvaII GGWCC 5 cut(s) 46, 265, 345, 756, 997
AxyI CCTNAGG 1 cut(s) 447
BaeGI GKGCMC 1 cut(s) 1027
BaeI ACNNNNGTAYC 2 cut(s) 73, 106
BalI TGGCCA 1 cut(s) 647
BanI GGYRCC 3 cut(s) 270, 1171, 1292
BanII GRGCYC 1 cut(s) 1027
BbsI GAAGAC 2 cut(s) 825, 1706
BbvI GCAGC 8 cut(s) 4, 32, 58, 370, 487, 1102, 1342, 1726
BccI CCATC 2 cut(s) 102, 353
BceAI ACGGC 3 cut(s) 136, 247, 564
BcgI CGANNNNNNTGC 4 cut(s) 302, 336, 482, 516
BciT130I CCWGG 1 cut(s) 1299
BcnI CCSGG 5 cut(s) 207, 1075, 1076, 1215, 1216
BfaI CTAG 6 cut(s) 782, 987, 1043, 1094, 1227, 1286
BfmI CTRYAG 3 cut(s) 18, 82, 1356
BfoI RGCGCY 4 cut(s) 148, 222, 274, 1296
BglI GCCNNNNNGGC 2 cut(s) 410, 467
BlpI GCTNAGC 1 cut(s) 1631
Bme1390I CCNGG 6 cut(s) 207, 1075, 1076, 1215, 1216, 1299
Bme18I GGWCC 5 cut(s) 46, 265, 345, 756, 997
BmeT110I CYCGRG 3 cut(s) 148, 1074, 1214
BmiI GGNNCC 6 cut(s) 47, 272, 998, 1025, 1173, 1294
BmrFI CCNGG 6 cut(s) 207, 1075, 1076, 1215, 1216, 1299
BmsI GCATC 3 cut(s) 71, 219, 546
BpiI GAAGAC 2 cut(s) 825, 1706
BplI GAGNNNNNCTC 2 cut(s) 1543, 1575
BpmI CTGGAG 1 cut(s) 269
Bpu10I CCTNAGC 2 cut(s) 341, 1374
Bpu1102I GCTNAGC 1 cut(s) 1631
Bpu14I TTCGAA 1 cut(s) 27
BpuMI CCSGG 5 cut(s) 207, 1075, 1076, 1215, 1216
BsaHI GRCGYC 4 cut(s) 57, 271, 524, 1293
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 1763, 1793
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 1215, 1245, 1276, 1513, 1759
Bse118I RCCGGY 2 cut(s) 267, 273
Bse1I ACTGG 3 cut(s) 713, 959, 1399
Bse21I CCTNAGG 1 cut(s) 447
Bse3DI GCAATG 3 cut(s) 876, 1036, 1317
BseBI CCWGG 1 cut(s) 1299
BseGI GGATG 4 cut(s) 62, 364, 978, 1618
BseLI CCNNNNNNNGG 6 cut(s) 247, 1215, 1245, 1276, 1513, 1759
BseMI GCAATG 3 cut(s) 876, 1036, 1317
BseMII CTCAG 6 cut(s) 305, 355, 620, 857, 911, 1365
BseNI ACTGG 3 cut(s) 713, 959, 1399
BseRI GAGGAG 2 cut(s) 422, 1527
BseSI GKGCMC 1 cut(s) 1027
BseX3I CGGCCG 1 cut(s) 577
BseXI GCAGC 8 cut(s) 4, 32, 58, 370, 487, 1102, 1342, 1726
BsgI GTGCAG 1 cut(s) 842
Bsh1236I CGCG 5 cut(s) 140, 215, 566, 1623, 1796
Bsh1285I CGRYCG 3 cut(s) 245, 580, 1281
BshFI GGCC 9 cut(s) 299, 404, 461, 579, 647, 734, 1025, 1238, 1709
BshNI GGYRCC 3 cut(s) 270, 1171, 1292
BsiEI CGRYCG 3 cut(s) 245, 580, 1281
BsiHKCI CYCGRG 3 cut(s) 148, 1074, 1214
BsiSI CCGG 8 cut(s) 207, 241, 268, 274, 326, 798, 1075, 1215
BsiWI CGTACG 1 cut(s) 528
BslFI GGGAC 6 cut(s) 59, 188, 1010, 1057, 1460, 1735
BslI CCNNNNNNNGG 6 cut(s) 247, 1215, 1245, 1276, 1513, 1759
BsmFI GGGAC 6 cut(s) 59, 188, 1010, 1057, 1460, 1735
BsmI GAATGC 4 cut(s) 503, 584, 768, 1219
BsnI GGCC 9 cut(s) 299, 404, 461, 579, 647, 734, 1025, 1238, 1709
BsoBI CYCGRG 3 cut(s) 148, 1074, 1214
Bsp119I TTCGAA 1 cut(s) 27
Bsp120I GGGCCC 1 cut(s) 1023
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 1615
Bsp143I GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
Bsp1720I GCTNAGC 1 cut(s) 1631
Bsp19I CCATGG 1 cut(s) 614
Bsp68I TCGCGA 1 cut(s) 215
BspANI GGCC 9 cut(s) 299, 404, 461, 579, 647, 734, 1025, 1238, 1709
BspCNI CTCAG 6 cut(s) 304, 354, 619, 856, 912, 1366
BspFNI CGCG 5 cut(s) 140, 215, 566, 1623, 1796
BspLI GGNNCC 6 cut(s) 47, 272, 998, 1025, 1173, 1294
BspMAI CTGCAG 2 cut(s) 22, 1360
BspPI GGATC 3 cut(s) 719, 1086, 1538
BspT104I TTCGAA 1 cut(s) 27
BspT107I GGYRCC 3 cut(s) 270, 1171, 1292
BsrDI GCAATG 3 cut(s) 876, 1036, 1317
BsrFI RCCGGY 2 cut(s) 267, 273
BsrGI TGTACA 1 cut(s) 1615
BsrI ACTGG 3 cut(s) 713, 959, 1399
BssAI RCCGGY 2 cut(s) 267, 273
BssMI GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
BssNAI GTATAC 1 cut(s) 1370
BssNI GRCGYC 4 cut(s) 57, 271, 524, 1293
BssT1I CCWWGG 4 cut(s) 614, 736, 1119, 1725
Bst1107I GTATAC 1 cut(s) 1370
Bst2UI CCWGG 1 cut(s) 1299
Bst4CI ACNGT 2 cut(s) 258, 1071
BstACI GRCGYC 4 cut(s) 57, 271, 524, 1293
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 1615
BstBI TTCGAA 1 cut(s) 27
BstC8I GCNNGC 8 cut(s) 125, 217, 275, 459, 654, 1017, 1027, 1291
BstDSI CCRYGG 2 cut(s) 118, 614
BstEII GGTNACC 1 cut(s) 133
BstF5I GGATG 4 cut(s) 62, 364, 978, 1618
BstFNI CGCG 5 cut(s) 140, 215, 566, 1623, 1796
BstH2I RGCGCY 4 cut(s) 148, 222, 274, 1296
BstHHI GCGC 6 cut(s) 147, 221, 273, 391, 1295, 1578
BstKTI GATC 6 cut(s) 335, 480, 496, 714, 1081, 1546
BstMBI GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
BstMCI CGRYCG 3 cut(s) 245, 580, 1281
BstNI CCWGG 1 cut(s) 1299
BstNSI RCATGY 2 cut(s) 1019, 1427
BstPI GGTNACC 1 cut(s) 133
BstSCI CCNGG 6 cut(s) 205, 1073, 1074, 1213, 1214, 1297
BstSFI CTRYAG 3 cut(s) 18, 82, 1356
BstSLI GKGCMC 1 cut(s) 1027
BstUI CGCG 5 cut(s) 140, 215, 566, 1623, 1796
BstV1I GCAGC 8 cut(s) 4, 32, 58, 370, 487, 1102, 1342, 1726
BstV2I GAAGAC 2 cut(s) 825, 1706
BstX2I RGATCY 1 cut(s) 1078
BstYI RGATCY 1 cut(s) 1078
BstZ17I GTATAC 1 cut(s) 1370
BstZI CGGCCG 1 cut(s) 577
Bsu36I CCTNAGG 1 cut(s) 447
BsuRI GGCC 9 cut(s) 299, 404, 461, 579, 647, 734, 1025, 1238, 1709
BtgI CCRYGG 2 cut(s) 118, 614
BtgZI GCGATG 2 cut(s) 196, 387
BtsCI GGATG 4 cut(s) 62, 364, 978, 1618
BtsI GCAGTG 1 cut(s) 1448
BtsIMutI CAGTG 2 cut(s) 917, 1448
BtuMI TCGCGA 1 cut(s) 215
Cac8I GCNNGC 8 cut(s) 125, 217, 275, 459, 654, 1017, 1027, 1291
CfoI GCGC 6 cut(s) 147, 221, 273, 391, 1295, 1578
Cfr10I RCCGGY 2 cut(s) 267, 273
Cfr9I CCCGGG 2 cut(s) 1074, 1214
CseI GACGC 2 cut(s) 65, 513
Csp6I GTAC 4 cut(s) 90, 529, 1128, 1616
CviAII CATG 6 cut(s) 615, 1016, 1020, 1424, 1747, 1759
CviQI GTAC 4 cut(s) 90, 529, 1128, 1616
DinI GGCGCC 2 cut(s) 272, 1294
DpnI GATC 6 cut(s) 334, 479, 495, 713, 1080, 1545
DpnII GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
EaeI YGGCCR 3 cut(s) 459, 577, 645
EagI CGGCCG 1 cut(s) 577
EciI GGCGGA 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 577
Eco130I CCWWGG 4 cut(s) 614, 736, 1119, 1725
Eco24I GRGCYC 1 cut(s) 1027
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 5 cut(s) 46, 265, 345, 756, 997
Eco52I CGGCCG 1 cut(s) 577
Eco57I CTGAAG 2 cut(s) 702, 1511
Eco81I CCTNAGG 1 cut(s) 447
Eco88I CYCGRG 3 cut(s) 148, 1074, 1214
Eco91I GGTNACC 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 733, 997
EcoO65I GGTNACC 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 1104
EcoRII CCWGG 1 cut(s) 1297
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 4 cut(s) 614, 736, 1119, 1725
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 1 cut(s) 1027
EgeI GGCGCC 2 cut(s) 272, 1294
EheI GGCGCC 2 cut(s) 272, 1294
ErhI CCWWGG 4 cut(s) 614, 736, 1119, 1725
FaeI CATG 6 cut(s) 618, 1019, 1023, 1427, 1750, 1762
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FaqI GGGAC 6 cut(s) 59, 188, 1010, 1057, 1460, 1735
FatI CATG 6 cut(s) 614, 1015, 1019, 1423, 1746, 1758
FauI CCCGC 2 cut(s) 1034, 1801
FblI GTMKAC 2 cut(s) 306, 1369
FokI GGATG 4 cut(s) 49, 371, 985, 1625
FriOI GRGCYC 1 cut(s) 1027
FspBI CTAG 6 cut(s) 782, 987, 1043, 1094, 1227, 1286
FspI TGCGCA 1 cut(s) 1577
GlaI GCGC 6 cut(s) 146, 220, 272, 390, 1294, 1577
GsuI CTGGAG 1 cut(s) 269
HaeII RGCGCY 4 cut(s) 148, 222, 274, 1296
HaeIII GGCC 9 cut(s) 299, 404, 461, 579, 647, 734, 1025, 1238, 1709
HapII CCGG 8 cut(s) 207, 241, 268, 274, 326, 798, 1075, 1215
HgaI GACGC 2 cut(s) 65, 513
HhaI GCGC 6 cut(s) 147, 221, 273, 391, 1295, 1578
Hin1I GRCGYC 4 cut(s) 57, 271, 524, 1293
Hin1II CATG 6 cut(s) 618, 1019, 1023, 1427, 1750, 1762
Hin6I GCGC 6 cut(s) 145, 219, 271, 389, 1293, 1576
HinP1I GCGC 6 cut(s) 145, 219, 271, 389, 1293, 1576
HincII GTYRAC 2 cut(s) 307, 706
HindII GTYRAC 2 cut(s) 307, 706
HindIII AAGCTT 3 cut(s) 650, 953, 1628
HinfI GANTC 9 cut(s) 29, 164, 280, 533, 635, 1556, 1686, 1702, 1763
HpaII CCGG 8 cut(s) 207, 241, 268, 274, 326, 798, 1075, 1215
HphI GGTGA 3 cut(s) 127, 631, 1613
Hpy166II GTNNAC 6 cut(s) 307, 691, 706, 1370, 1456, 1618
Hpy188I TCNGA 6 cut(s) 192, 262, 294, 349, 682, 1491
Hpy188III TCNNGA 4 cut(s) 214, 284, 898, 1541
Hpy8I GTNNAC 6 cut(s) 307, 691, 706, 1370, 1456, 1618
Hpy99I CGWCG 6 cut(s) 59, 317, 353, 356, 485, 529
HpyAV CCTTC 9 cut(s) 124, 154, 394, 532, 808, 1150, 1188, 1507, 1582
HpyCH4III ACNGT 2 cut(s) 258, 1071
HpyCH4IV ACGT 1 cut(s) 396
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 4 cut(s) 57, 271, 524, 1293
Hsp92II CATG 6 cut(s) 618, 1019, 1023, 1427, 1750, 1762
HspAI GCGC 6 cut(s) 145, 219, 271, 389, 1293, 1576
KasI GGCGCC 2 cut(s) 270, 1292
KroI GCCGGC 1 cut(s) 273
KroNI GCCGGC 1 cut(s) 275
Kzo9I GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
LmnI GCTCC 2 cut(s) 328, 1162
Lsp1109I GCAGC 8 cut(s) 4, 32, 58, 370, 487, 1102, 1342, 1726
LweI GCATC 3 cut(s) 71, 219, 546
MaeI CTAG 6 cut(s) 782, 987, 1043, 1094, 1227, 1286
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 3 cut(s) 133, 451, 1204
MalI GATC 6 cut(s) 334, 479, 495, 713, 1080, 1545
MboI GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
MflI RGATCY 1 cut(s) 1078
MhlI GDGCHC 1 cut(s) 1027
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 4 cut(s) 928, 963, 1104, 1398
MluNI TGGCCA 1 cut(s) 647
Mly113I GGCGCC 2 cut(s) 271, 1293
MlyI GAGTC 3 cut(s) 542, 644, 1696
MmeI TCCRAC 4 cut(s) 240, 372, 1289, 1755
Mox20I TGGCCA 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 1021
MreI CGCCGGCG 1 cut(s) 273
MroNI GCCGGC 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 626
MscI TGGCCA 1 cut(s) 647
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 2 cut(s) 71, 553
MspI CCGG 8 cut(s) 207, 241, 268, 274, 326, 798, 1075, 1215
MspR9I CCNGG 6 cut(s) 207, 1075, 1076, 1215, 1216, 1299
Mva1269I GAATGC 4 cut(s) 503, 584, 768, 1219
MvaI CCWGG 1 cut(s) 1299
MvnI CGCG 5 cut(s) 140, 215, 566, 1623, 1796
NaeI GCCGGC 1 cut(s) 275
NarI GGCGCC 2 cut(s) 271, 1293
NciI CCSGG 5 cut(s) 207, 1075, 1076, 1215, 1216
NcoI CCATGG 1 cut(s) 614
NdeII GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
NgoMIV GCCGGC 1 cut(s) 273
NlaIII CATG 6 cut(s) 618, 1019, 1023, 1427, 1750, 1762
NlaIV GGNNCC 6 cut(s) 47, 272, 998, 1025, 1173, 1294
NmeAIII GCCGAG 2 cut(s) 430, 487
NmuCI GTSAC 2 cut(s) 133, 451
NruI TCGCGA 1 cut(s) 215
NsbI TGCGCA 1 cut(s) 1577
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 2 cut(s) 1019, 1427
NspV TTCGAA 1 cut(s) 27
PaeI GCATGC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 6 cut(s) 111, 231, 312, 402, 489, 533
PctI GAATGC 4 cut(s) 503, 584, 768, 1219
PdiI GCCGGC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 626
PfeI GAWTC 6 cut(s) 29, 164, 280, 1556, 1686, 1763
Pfl23II CGTACG 1 cut(s) 528
PleI GAGTC 3 cut(s) 541, 643, 1696
PluTI GGCGCC 2 cut(s) 274, 1296
PpsI GAGTC 3 cut(s) 541, 643, 1696
PpuMI RGGWCCY 1 cut(s) 997
Psp5II RGGWCCY 1 cut(s) 997
Psp6I CCWGG 1 cut(s) 1297
PspEI GGTNACC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 1297
PspLI CGTACG 1 cut(s) 528
PspN4I GGNNCC 6 cut(s) 47, 272, 998, 1025, 1173, 1294
PspOMI GGGCCC 1 cut(s) 1023
PspPPI RGGWCCY 1 cut(s) 997
PstI CTGCAG 2 cut(s) 22, 1360
PsuI RGATCY 1 cut(s) 1078
PvuII CAGCTG 1 cut(s) 553
RruI TCGCGA 1 cut(s) 215
RsaI GTAC 4 cut(s) 91, 530, 1129, 1617
RsaNI GTAC 4 cut(s) 90, 529, 1128, 1616
SalI GTCGAC 1 cut(s) 305
Sau3AI GATC 6 cut(s) 332, 477, 493, 711, 1078, 1543
SchI GAGTC 3 cut(s) 542, 644, 1696
ScrFI CCNGG 6 cut(s) 207, 1075, 1076, 1215, 1216, 1299
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 3 cut(s) 71, 219, 546
SfcI CTRYAG 3 cut(s) 18, 82, 1356
SfoI GGCGCC 2 cut(s) 272, 1294
SfuI TTCGAA 1 cut(s) 27
SgrAI CRCCGGYG 1 cut(s) 273
SinI GGWCC 5 cut(s) 46, 265, 345, 756, 997
SmaI CCCGGG 2 cut(s) 1076, 1216
SphI GCATGC 1 cut(s) 1019
Sse9I AATT 4 cut(s) 928, 963, 1104, 1398
SspDI GGCGCC 2 cut(s) 270, 1292
SspMI CTAG 6 cut(s) 782, 987, 1043, 1094, 1227, 1286
StyD4I CCNGG 6 cut(s) 205, 1073, 1074, 1213, 1214, 1297
StyI CCWWGG 4 cut(s) 614, 736, 1119, 1725
TaaI ACNGT 2 cut(s) 258, 1071
TaiI ACGT 1 cut(s) 399
TasI AATT 4 cut(s) 928, 963, 1104, 1398
TatI WGTACW 1 cut(s) 1615
TauI GCSGC 4 cut(s) 62, 389, 470, 473
TfiI GAWTC 6 cut(s) 29, 164, 280, 1556, 1686, 1763
TscAI CASTG 2 cut(s) 924, 1448
TseFI GTSAC 2 cut(s) 133, 451
TseI GCWGC 8 cut(s) 17, 20, 71, 383, 500, 1090, 1355, 1739
Tsp45I GTSAC 2 cut(s) 133, 451
TspDTI ATGAA 4 cut(s) 393, 1328, 1393, 1401
TspGWI ACGGA 2 cut(s) 240, 715
TspMI CCCGGG 2 cut(s) 1074, 1214
TspRI CASTG 2 cut(s) 924, 1448
VpaK11BI GGWCC 5 cut(s) 46, 265, 345, 756, 997
XapI RAATTY 3 cut(s) 928, 1104, 1398
XceI RCATGY 2 cut(s) 1019, 1427
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XmaI CCCGGG 2 cut(s) 1074, 1214
XmiI GTMKAC 2 cut(s) 306, 1369
XmnI GAANNNNTTC 1 cut(s) 626
XspI CTAG 6 cut(s) 782, 987, 1043, 1094, 1227, 1286
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.