Rh3BG343900

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0002090
Physical Location & Seq
Forward (+)
37193915 .. 37199097
5183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG343900.1

Sequence Viewer

Length: 1779 bp
ATGGACGAGAATTCAGCACTGATCGAATCGATTCTGAGAGAGGAAGACGAGGCGGAGGCAGACAAATATGTAAAGAAGATGAGCAGCACTGCGCCGGTCCAAAACGGCGACGCTTACGCCTGGCAAACTGTTTCTTATCACAAGCGAAACCGACGACTTTCTCAGCCAATTCCGCCTGATTCTGTCGGCGATCCCTCCAACGGCGACGTCTTCCGCTCGATCGAGCTGCATTCCGAGGACCGCCGGAGGAGAATTCGACAGGCTCAAACGGATTCCCTTCCCGCTGTCCGATCCGAGCCGCATTCCGACGACGATAGCGATGCCGAGGTGGCCGCTGCCGCGGAAAATGGCGAGCCGAAGAAGGCCAAAGTGAAGAAGCCGAAGAAGCCAAAGCTGACTGTGGCCGAGGCGGCTTCCAAGATCGACGCGGCTCATCTCGGTGCTTTTCTCGCCGAGATAACGGAGGTGTATCAATCCCAACAAGACATACAGCTTTTGCGTCTGGCTGATTATTTCGGTCGAGCATTTGCGTCCGTCACTGCCGCTCAGTTTCCCTGGTTGAAGATATTCAAGGAATCCACTGTCTCAAAGATGGTTGACATCCCTCTTTCTCATATATCTGAAAATGTTTATAAGACATCAGCTGACTGGATCGGCCAGCGCTCCACTGAAGCCCTTGGGTCCTTCGTGTTGTGGTCACTAGATTGCTTTCTTGCTGACCTTGCAAGTCATCAGGGAGCAGCAAAGGGATCCAAAAAGGTGGTCCAGCAACCTTCAAAATGTCAGGTTGCTATATTTGCGGTTTTAGCAATGGTGTTGCGAAAAAAACCTGATGTATTGTTTAGTCTATTTCCTGTGATGAGGGAAAGTTTGAAATATCAAGGCCAAGATAAGCTTCCGCTCACAGTGTGGTTGATTACGCAGGCCTCTCAAGGAGATCTGGTTGTTGGGTTGTACACATGGGTACATCTTCTCCTGCCTATATTGAGTAGCAAGTCAGGTTGTAATCCACAGTCTAGAGACTTGATTTTGCAGTCAGTGGAAAGAATTTTATCATCTCCAAAAGCTCGGCCTATTCTATTAAATGGTGCTGTTAGGAAAGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTTTCTTTTCCTTCACCTTCAGTGCGAGTTAAGGCAACTGAGAGATTTGAAGCTGTCTATCCAATCTTGAAAGAGATTGCCCTTGCTGGATCCCCAGGAAGCAAAACAGTGAGGCAGGTTGCACAGAAGACACTGAATTATTCAGTTAAAGCTGTTAAAGAAGGCATCCCCGATCTCTCTGAGGAGGCAACCGCTATATTTATCTGGTGTTTGAAACAAAGTCCCGAATGTTACAAGCAATGGGACATGCTTTATCTGGACAATCTTGATGCAAGTGTTATTGTGCTGAAAAAGTTATCTGACGAGTGGAAGGATCACTCCGTTAAACATGCCTCTCTTGACCCCTTGAGGGAAACCTTGAAGAGTTTCAGGGAGAAGAATGAAAAAGCATTGGCAAAAGGAGATGATTTGGTGCGCCATTCGTTGTTGAAGGATGCAGATAAATATTGCAAGCTGATTTTAGGACGGTCATCTCAAGGCCGTGGATGCATGAAAAGCATGGTTCTGGTGTCTGTTGCCCTTGCTATGGTTGCTGCTATCATGTCCCAAAACATACAACCCGAAGACCTCAAGAAACTTGCAGCCATGTTTAACTTCCCCCTGACTCTAAATGGAGGAATTCAGCAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

592

Amino Acids

65.68

Weight (kDa)

8.92

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 89 - 569 4.4e-24 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 633
AatII GACGTC 1 cut(s) 210
Acc36I ACCTGC 1 cut(s) 1252
AccBSI CCGCTC 3 cut(s) 216, 545, 901
AccII CGCG 2 cut(s) 341, 428
AclWI GGATC 8 cut(s) 185, 285, 659, 744, 757, 1230, 1243, 1467
AcoI YGGCCR 3 cut(s) 330, 402, 655
AcsI RAATTY 4 cut(s) 10, 252, 1047, 1764
AcuI CTGAAG 2 cut(s) 690, 1149
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 2 cut(s) 909, 1114
AfaI GTAC 2 cut(s) 956, 966
AfeI AGCGCT 1 cut(s) 662
AfiI CCNNNNNNNGG 3 cut(s) 200, 1495, 1672
AgsI TTSAA 9 cut(s) 562, 571, 777, 874, 1196, 1216, 1360, 1507, 1576
AjnI CCWGG 3 cut(s) 119, 554, 1240
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 9 cut(s) 226, 394, 493, 644, 895, 1067, 1199, 1298, 1600
AluI AGCT 9 cut(s) 226, 394, 493, 644, 895, 1067, 1199, 1298, 1600
Alw21I GWGCWC 1 cut(s) 1110
Alw26I GTCTC 2 cut(s) 589, 1014
AlwI GGATC 8 cut(s) 185, 285, 659, 744, 757, 1230, 1243, 1467
Aor51HI AGCGCT 1 cut(s) 662
AoxI GGCC 8 cut(s) 330, 363, 402, 655, 883, 924, 1070, 1624
ApeKI GCWGC 7 cut(s) 84, 226, 335, 740, 1679, 1727, 1771
ApoI RAATTY 4 cut(s) 10, 252, 1047, 1764
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 3 cut(s) 30, 566, 1511
AspLEI GCGC 3 cut(s) 94, 663, 1563
AspS9I GGNCC 4 cut(s) 97, 238, 681, 763
AsuHPI GGTGA 1 cut(s) 1152
AvaII GGWCC 4 cut(s) 97, 238, 681, 763
BamHI GGATCC 2 cut(s) 749, 1235
BbsI GAAGAC 4 cut(s) 51, 202, 1280, 1716
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
BccI CCATC 2 cut(s) 586, 1129
BceAI ACGGC 3 cut(s) 121, 217, 1611
BcgI CGANNNNNNTGC 4 cut(s) 208, 242, 302, 336
BciT130I CCWGG 3 cut(s) 121, 556, 1242
BcoDI GTCTC 2 cut(s) 589, 1014
BfaI CTAG 2 cut(s) 701, 1017
BfoI RGCGCY 1 cut(s) 664
BfuAI ACCTGC 1 cut(s) 1252
BglI GCCNNNNNGGC 2 cut(s) 329, 410
BglII AGATCT 1 cut(s) 937
Bme1390I CCNGG 3 cut(s) 121, 556, 1242
Bme18I GGWCC 4 cut(s) 97, 238, 681, 763
BmgT120I GGNCC 4 cut(s) 97, 238, 681, 763
BmiI GGNNCC 3 cut(s) 682, 751, 1237
BmrFI CCNGG 3 cut(s) 121, 556, 1242
BmsI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
BpiI GAAGAC 4 cut(s) 51, 202, 1280, 1716
BpuEI CTTGAG 4 cut(s) 915, 1513, 1605, 1700
Bsa29I ATCGAT 1 cut(s) 29
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 8 cut(s) 234, 324, 339, 405, 554, 676, 1240, 1627
BsaXI ACNNNNNCTCC 4 cut(s) 927, 957, 957, 987
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 1495, 1672
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 653
Bse3DI GCAATG 2 cut(s) 816, 1391
BseBI CCWGG 3 cut(s) 121, 556, 1242
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 8 cut(s) 234, 324, 339, 405, 554, 676, 1240, 1627
BseGI GGATG 4 cut(s) 600, 1311, 1585, 1637
BseLI CCNNNNNNNGG 3 cut(s) 200, 1495, 1672
BseMI GCAATG 2 cut(s) 816, 1391
BseMII CTCAG 5 cut(s) 26, 176, 560, 1176, 1317
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 2 cut(s) 262, 1343
BseXI GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
Bsh1236I CGCG 2 cut(s) 341, 428
Bsh1285I CGRYCG 2 cut(s) 222, 520
BshFI GGCC 8 cut(s) 332, 365, 404, 657, 885, 926, 1072, 1626
BshVI ATCGAT 1 cut(s) 29
BsiEI CGRYCG 2 cut(s) 222, 520
BsiHKAI GWGCWC 1 cut(s) 1110
BsiSI CCGG 2 cut(s) 95, 244
BslFI GGGAC 3 cut(s) 1353, 1403, 1675
BslI CCNNNNNNNGG 3 cut(s) 200, 1495, 1672
BsmAI GTCTC 2 cut(s) 589, 1014
BsmFI GGGAC 3 cut(s) 1353, 1403, 1675
BsmI GAATGC 2 cut(s) 229, 301
BsnI GGCC 8 cut(s) 332, 365, 404, 657, 885, 926, 1072, 1626
Bsp1286I GDGCHC 1 cut(s) 1110
Bsp1407I TGTACA 1 cut(s) 954
BspANI GGCC 8 cut(s) 332, 365, 404, 657, 885, 926, 1072, 1626
BspCNI CTCAG 5 cut(s) 27, 175, 559, 1177, 1318
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 2 cut(s) 341, 428
BspLI GGNNCC 3 cut(s) 682, 751, 1237
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 8 cut(s) 185, 285, 659, 744, 757, 1230, 1243, 1467
BsrBI CCGCTC 3 cut(s) 216, 545, 901
BsrDI GCAATG 2 cut(s) 816, 1391
BsrFI RCCGGY 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 954
BsrI ACTGG 1 cut(s) 653
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 8 cut(s) 234, 324, 339, 405, 554, 676, 1240, 1627
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 676
Bst2UI CCWGG 3 cut(s) 121, 556, 1242
Bst4CI ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
Bst6I CTCTTC 1 cut(s) 1502
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 1 cut(s) 1114
BstAUI TGTACA 1 cut(s) 954
BstC8I GCNNGC 4 cut(s) 353, 659, 924, 1598
BstDEI CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
BstDSI CCRYGG 2 cut(s) 339, 1627
BstF5I GGATG 4 cut(s) 600, 1311, 1585, 1637
BstFNI CGCG 2 cut(s) 341, 428
BstH2I RGCGCY 1 cut(s) 664
BstHHI GCGC 3 cut(s) 94, 663, 1563
BstMAI GTCTC 2 cut(s) 589, 1014
BstMCI CGRYCG 2 cut(s) 222, 520
BstNI CCWGG 3 cut(s) 121, 556, 1242
BstNSI RCATGY 2 cut(s) 1396, 1478
BstSCI CCNGG 3 cut(s) 119, 554, 1240
BstUI CGCG 2 cut(s) 341, 428
BstV1I GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
BstV2I GAAGAC 4 cut(s) 51, 202, 1280, 1716
BstX2I RGATCY 3 cut(s) 749, 937, 1235
BstXI CCANNNNNNTGG 1 cut(s) 760
BstYI RGATCY 3 cut(s) 749, 937, 1235
Bsu15I ATCGAT 1 cut(s) 29
BsuRI GGCC 8 cut(s) 332, 365, 404, 657, 885, 926, 1072, 1626
BsuTUI ATCGAT 1 cut(s) 29
BtgI CCRYGG 2 cut(s) 339, 1627
BtgZI GCGATG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 600, 1311, 1585, 1637
BtsI GCAGTG 2 cut(s) 87, 537
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 4 cut(s) 353, 659, 924, 1598
CfoI GCGC 3 cut(s) 94, 663, 1563
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 4 cut(s) 97, 238, 681, 763
Cfr42I CCGCGG 1 cut(s) 342
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 4 cut(s) 119, 434, 488, 519
Csp6I GTAC 2 cut(s) 955, 965
CviAII CATG 7 cut(s) 960, 1393, 1475, 1636, 1645, 1687, 1732
CviQI GTAC 2 cut(s) 955, 965
DdeI CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
DraIII CACNNNGTG 2 cut(s) 909, 1114
EaeI YGGCCR 3 cut(s) 330, 402, 655
Eam1104I CTCTTC 1 cut(s) 1502
EarI CTCTTC 1 cut(s) 1502
EciI GGCGGA 2 cut(s) 68, 162
Eco130I CCWWGG 1 cut(s) 676
Eco147I AGGCCT 1 cut(s) 926
Eco47I GGWCC 4 cut(s) 97, 238, 681, 763
Eco47III AGCGCT 1 cut(s) 662
Eco57I CTGAAG 2 cut(s) 690, 1149
EcoO109I RGGNCCY 1 cut(s) 681
EcoRI GAATTC 3 cut(s) 10, 252, 1764
EcoRII CCWGG 3 cut(s) 119, 554, 1240
EcoT14I CCWWGG 1 cut(s) 676
EcoT22I ATGCAT 1 cut(s) 1637
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 7 cut(s) 963, 1396, 1478, 1639, 1648, 1690, 1735
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 3 cut(s) 1353, 1403, 1675
FatI CATG 7 cut(s) 959, 1392, 1474, 1635, 1644, 1686, 1731
FauI CCCGC 1 cut(s) 289
FokI GGATG 4 cut(s) 587, 1298, 1592, 1644
FspBI CTAG 2 cut(s) 701, 1017
GlaI GCGC 3 cut(s) 93, 662, 1562
HaeII RGCGCY 1 cut(s) 664
HaeIII GGCC 8 cut(s) 332, 365, 404, 657, 885, 926, 1072, 1626
HapII CCGG 2 cut(s) 95, 244
HgaI GACGC 4 cut(s) 119, 434, 488, 519
HhaI GCGC 3 cut(s) 94, 663, 1563
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 7 cut(s) 963, 1396, 1478, 1639, 1648, 1690, 1735
Hin6I GCGC 3 cut(s) 92, 661, 1561
HinP1I GCGC 3 cut(s) 92, 661, 1561
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 893
HinfI GANTC 6 cut(s) 26, 31, 179, 272, 575, 1750
HpaII CCGG 2 cut(s) 95, 244
HphI GGTGA 1 cut(s) 1152
Hpy166II GTNNAC 2 cut(s) 598, 957
Hpy188I TCNGA 8 cut(s) 36, 235, 290, 295, 307, 622, 1327, 1447
Hpy188III TCNNGA 8 cut(s) 1017, 1138, 1213, 1370, 1403, 1412, 1484, 1717
Hpy8I GTNNAC 2 cut(s) 598, 957
Hpy99I CGWCG 6 cut(s) 113, 156, 209, 311, 314, 428
HpyAV CCTTC 9 cut(s) 287, 355, 694, 783, 1167, 1173, 1301, 1450, 1570
HpyCH4III ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 9 cut(s) 229, 725, 1033, 1268, 1418, 1583, 1596, 1635, 1727
HpyF3I CTNAG 5 cut(s) 35, 162, 546, 1185, 1326
HpySE526I ACGT 1 cut(s) 207
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 7 cut(s) 963, 1396, 1478, 1639, 1648, 1690, 1735
HspAI GCGC 3 cut(s) 92, 661, 1561
KspI CCGCGG 1 cut(s) 342
LmnI GCTCC 3 cut(s) 668, 737, 1105
Lsp1109I GCAGC 6 cut(s) 96, 213, 322, 752, 1666, 1739
LweI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
MaeI CTAG 2 cut(s) 701, 1017
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 3 cut(s) 535, 696, 1376
MbiI CCGCTC 3 cut(s) 216, 545, 901
MflI RGATCY 3 cut(s) 749, 937, 1235
MhlI GDGCHC 1 cut(s) 1110
MluCI AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1764
MlyI GAGTC 1 cut(s) 1744
MmeI TCCRAC 2 cut(s) 222, 330
Mph1103I ATGCAT 1 cut(s) 1637
MroXI GAANNNNTTC 3 cut(s) 30, 566, 1511
MseI TTAA 6 cut(s) 1082, 1176, 1293, 1302, 1470, 1737
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 4 cut(s) 284, 335, 341, 644
MspI CCGG 2 cut(s) 95, 244
MspR9I CCNGG 3 cut(s) 121, 556, 1242
Mva1269I GAATGC 2 cut(s) 229, 301
MvaI CCWGG 3 cut(s) 121, 556, 1242
MvnI CGCG 2 cut(s) 341, 428
NlaIII CATG 7 cut(s) 963, 1396, 1478, 1639, 1648, 1690, 1735
NlaIV GGNNCC 3 cut(s) 682, 751, 1237
NmeAIII GCCGAG 4 cut(s) 349, 430, 478, 1048
NmuCI GTSAC 2 cut(s) 535, 696
NsiI ATGCAT 1 cut(s) 1637
NspI RCATGY 2 cut(s) 1396, 1478
PceI AGGCCT 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 2 cut(s) 229, 301
PdmI GAANNNNTTC 3 cut(s) 30, 566, 1511
PfeI GAWTC 5 cut(s) 26, 31, 179, 272, 575
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 1 cut(s) 1744
PpsI GAGTC 1 cut(s) 1744
PpuMI RGGWCCY 1 cut(s) 681
PsiI TTATAA 1 cut(s) 633
Psp5II RGGWCCY 1 cut(s) 681
Psp6I CCWGG 3 cut(s) 119, 554, 1240
PspGI CCWGG 3 cut(s) 119, 554, 1240
PspN4I GGNNCC 3 cut(s) 682, 751, 1237
PspPI GGNCC 4 cut(s) 97, 238, 681, 763
PspPPI RGGWCCY 1 cut(s) 681
PsuI RGATCY 3 cut(s) 749, 937, 1235
PvuI CGATCG 1 cut(s) 222
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 956, 966
RsaNI GTAC 2 cut(s) 955, 965
RseI CAYNNNNRTG 1 cut(s) 438
SacII CCGCGG 1 cut(s) 342
SaqAI TTAA 6 cut(s) 1082, 1176, 1293, 1302, 1470, 1737
Sau96I GGNCC 4 cut(s) 97, 238, 681, 763
SchI GAGTC 1 cut(s) 1744
ScrFI CCNGG 3 cut(s) 121, 556, 1242
SduI GDGCHC 1 cut(s) 1110
SfaNI GCATC 5 cut(s) 310, 1320, 1405, 1570, 1622
Sfr303I CCGCGG 1 cut(s) 342
SgrBI CCGCGG 1 cut(s) 342
SinI GGWCC 4 cut(s) 97, 238, 681, 763
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 4 cut(s) 930, 1492, 1620, 1715
SmoI CTYRAG 4 cut(s) 930, 1492, 1620, 1715
Sse9I AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1764
SseBI AGGCCT 1 cut(s) 926
SspI AATATT 1 cut(s) 1592
SspMI CTAG 2 cut(s) 701, 1017
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 3 cut(s) 119, 554, 1240
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 7 cut(s) 130, 400, 583, 907, 1014, 1255, 1614
TaiI ACGT 1 cut(s) 210
TaqI TCGA 7 cut(s) 24, 29, 218, 222, 256, 423, 520
TaqII GACCGA 1 cut(s) 506
TasI AATT 6 cut(s) 10, 168, 252, 1047, 1282, 1764
TatI WGTACW 1 cut(s) 954
TauI GCSGC 6 cut(s) 301, 335, 341, 413, 431, 545
TfiI GAWTC 5 cut(s) 26, 31, 179, 272, 575
Tru1I TTAA 6 cut(s) 1082, 1176, 1293, 1302, 1470, 1737
Tru9I TTAA 6 cut(s) 1082, 1176, 1293, 1302, 1470, 1737
TseFI GTSAC 2 cut(s) 535, 696
TseI GCWGC 7 cut(s) 84, 226, 335, 740, 1679, 1727, 1771
Tsp45I GTSAC 2 cut(s) 535, 696
TspDTI ATGAA 2 cut(s) 1542, 1652
TspGWI ACGGA 4 cut(s) 284, 476, 523, 1456
VpaK11BI GGWCC 4 cut(s) 97, 238, 681, 763
XapI RAATTY 4 cut(s) 10, 252, 1047, 1764
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 2 cut(s) 1396, 1478
XmnI GAANNNNTTC 3 cut(s) 30, 566, 1511
XspI CTAG 2 cut(s) 701, 1017
ZraI GACGTC 1 cut(s) 208
Zsp2I ATGCAT 1 cut(s) 1637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.