MD09G1264000.v1.1

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002090
Physical Location & Seq
Reverse (-)
33712952 .. 33717655
4704 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1264000.v1.1.491

Sequence Viewer

Length: 1800 bp
ATGGACGAAAGTTCATCGGCACTGATCGAAGCAATTCTCAGAGAGCAAGAGGAGGAGGAGGCACAGAGATATGGCAGAAAGCTCACCCAAAACAGCGACGCTTATGCGTGGCAGACGGTTTCGTATTCGAAGCGAAACCGGAAATCCTCCTCCAAGGCGCTTCCGGCGGACTCCAACGGCGATCTCCACGACGGTGTCGTATCCTCCGCCTCCGACGTGTTCCGGCCGATCGAGCTGCATTCCGAGGAGCGCCGTCGGAGAGTTCTCGAGGCCCAGGCCGCAGCCGTTTCCGGGGACGTCGCACCCGGCGCATCGAAGCGGACTTCCGATGACGACGATGAGGGTGATAGCGATGGTGAGGTGGCCGGGGCGGCCGTCGAAAACGGCGAGGTGAAGAAGGTGAAGCAGAAGAAGCCGAAGAAGCCGAAGGTGACGGTGGCGGAGGCAGCGTCGAAGATGGACGCCGGTGATCTCGGCGCCTTTCTGGCTGATATAACTGCTTCGTATAATGAGACGCAGCAGGACATACAGCTTATGCGTTTGGCGGATTATTTCGGTCGAGCATTTGCGGCGGTCAGTCCGGCTCAATTTCCGTGGTTGAAGACGTTCAAGGAATCCTCTGTGGCAAAGCTGGTTGATATCCCCCTTTCGCATGTATCTGATGATGTTTACAAGATATCAGTTGAATGGATCGGCCAGCGCTCTATTGAAGCGCTTGGATCGTTTGTGCTATGGTCGTTGGACAGCATTCTTGCTGACCTTGCAACTCATCAAGGAGTAGCCAAGGGATCCAAAAAGGCGGTCCAGCAAGCATCTTCAAAGTCTCAGGTTGCCATATTTGTGGTTTTAGCAATGGTATTGCGACGAAGACCTGAAGTACTGATTAGTTTACTTCCTGTAATGAGAGGAAGTCCGAAATATGAAGGCCAAGATAAGCTTCCAATCACAGTTTGGTTGGTTGCACAGGCTTCTCTAGGAGATCTGGTTGTTGGGTTGTACTTGTGGGTACAATTTCTCTTGCCTATATTGAGTAGCAAGTCAAGTAGTAATCCACTGTGTAGAGACTTAATTTTGCAGTCTGTGGAAAGAATTTTGTCTTACCCGAAAGCCCGTCCTATTCTATTAAATGGTGCTGTCAGAAAGGGGGAGCACATAGTGCCACCATCAGCCCTTGATCTCTTGATGAGAGTCTCTTTTCGGACACCTTCTGCCCGAGTTAAGGCAACTGAGAGGTTTGAAGCTGTTTATCCAACTTTGAAAGAGATCGCCCTTGCTGGTTCCCCTGGAAGCAAAACAATGAGGCAAGTCACACAGCAGATATTGAAGAATTCAGTTAAAGCTGTTAATGAGGGCATACCTGATCTGTCTAAGGAAGCAAGTGGCTTATTTATTTGGTGTTTGACGCAAAACCCTGAATGTTACAGGCAATGGGACATACTTTATCTGGACAATCTCGATGCAAGTGTTGTTCTCCTGAAAAAGATATCTGACGAGTGGAAGGAGCACTCTGTTAAACATGCTTCTCTTGACCCCTTGAGGGAAACTTTAAAGAGTTTCAGGGAGAAGAATGATAAAGCATTGGCAGCGGGAGATGATGTTGCCCGCCATTCACTGTTGAAGGATGCAGACAAATACTGCAAACAGATTTTGGGACGATTGTCGCAAGGCCATGGATGCATGAAGAGTATGGTACTTGTTTCTGTTGCCCTCGCTGTGGGTGCTGCGGTCATGTCCCAAAACATACAACCTGGGGACATTAAGAAATTTGTAGCTATGTTTAACTTACCCCCAACTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

600

Amino Acids

65.71

Weight (kDa)

8.65

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 131 - 574 9.4e-22 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 476
AclWI GGATC 4 cut(s) 698, 727, 783, 796
AcoI YGGCCR 4 cut(s) 224, 363, 372, 694
AcsI RAATTY 3 cut(s) 1089, 1327, 1763
AcuI CTGAAG 1 cut(s) 894
AcyI GRCGYC 3 cut(s) 297, 462, 477
AdeI CACNNNGTG 1 cut(s) 1156
AfaI GTAC 4 cut(s) 879, 998, 1008, 1692
AfeI AGCGCT 2 cut(s) 701, 714
AfiI CCNNNNNNNGG 4 cut(s) 291, 1219, 1537, 1714
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 9 cut(s) 601, 610, 686, 710, 819, 1238, 1258, 1324, 1618
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 3 cut(s) 273, 1282, 1747
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AleI CACNNNNGTG 1 cut(s) 192
AluBI AGCT 8 cut(s) 82, 235, 532, 631, 937, 1241, 1340, 1772
AluI AGCT 8 cut(s) 82, 235, 532, 631, 937, 1241, 1340, 1772
Alw21I GWGCWC 2 cut(s) 1152, 1506
Alw26I GTCTC 4 cut(s) 506, 828, 1056, 1195
AlwI GGATC 4 cut(s) 698, 727, 783, 796
Ama87I CYCGRG 2 cut(s) 266, 1212
Aor51HI AGCGCT 2 cut(s) 701, 714
AoxI GGCC 8 cut(s) 224, 270, 276, 363, 372, 694, 925, 1666
ApeKI GCWGC 6 cut(s) 235, 281, 446, 517, 1583, 1721
ApoI RAATTY 3 cut(s) 1089, 1327, 1763
ArsI GACNNNNNNTTYG 2 cut(s) 786, 818
Asp700I GAANNNNTTC 2 cut(s) 33, 605
AspLEI GCGC 6 cut(s) 160, 252, 311, 479, 702, 715
AspS9I GGNCC 2 cut(s) 271, 802
AsuC2I CCSGG 3 cut(s) 292, 306, 367
AsuHPI GGTGA 7 cut(s) 76, 356, 368, 403, 412, 442, 479
AsuII TTCGAA 1 cut(s) 128
AvaI CYCGRG 2 cut(s) 266, 1212
AvaII GGWCC 1 cut(s) 802
BamHI GGATCC 1 cut(s) 788
BanI GGYRCC 1 cut(s) 476
BbsI GAAGAC 2 cut(s) 608, 874
Bbv12I GWGCWC 2 cut(s) 1152, 1506
BbvI GCAGC 6 cut(s) 222, 293, 458, 529, 1595, 1708
BccI CCATC 3 cut(s) 347, 451, 1171
BceAI ACGGC 5 cut(s) 193, 237, 269, 359, 400
BcgI CGANNNNNNTGC 4 cut(s) 86, 120, 217, 251
BciT130I CCWGG 3 cut(s) 275, 1284, 1749
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 3 cut(s) 292, 306, 367
BcoDI GTCTC 4 cut(s) 506, 828, 1056, 1195
BfaI CTAG 1 cut(s) 974
BfoI RGCGCY 5 cut(s) 161, 253, 480, 703, 716
BfuI GTATCC 1 cut(s) 211
BglI GCCNNNNNGGC 2 cut(s) 371, 485
BglII AGATCT 1 cut(s) 979
BisI GCNGC 9 cut(s) 236, 279, 282, 372, 447, 518, 570, 1584, 1722
BlsI GCNGC 9 cut(s) 237, 280, 283, 373, 448, 519, 571, 1585, 1723
BmcAI AGTACT 1 cut(s) 879
Bme1390I CCNGG 6 cut(s) 275, 292, 306, 367, 1284, 1749
Bme18I GGWCC 1 cut(s) 802
BmeT110I CYCGRG 2 cut(s) 266, 1212
BmgBI CACGTC 1 cut(s) 217
BmgT120I GGNCC 2 cut(s) 271, 802
BmiI GGNNCC 3 cut(s) 478, 790, 1279
BmrFI CCNGG 6 cut(s) 275, 292, 306, 367, 1284, 1749
BmsI GCATC 5 cut(s) 320, 821, 1447, 1612, 1664
BoxI GACNNNNGTC 1 cut(s) 1657
BpiI GAAGAC 2 cut(s) 608, 874
Bpu14I TTCGAA 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 1555
BpuMI CCSGG 3 cut(s) 292, 306, 367
BsaHI GRCGYC 3 cut(s) 297, 462, 477
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 6 cut(s) 434, 464, 969, 999, 1581, 1611
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 1219, 1537, 1714
Bse118I RCCGGY 1 cut(s) 464
Bse3DI GCAATG 2 cut(s) 858, 1433
BseBI CCWGG 3 cut(s) 275, 1284, 1749
BseGI GGATG 2 cut(s) 1627, 1679
BseLI CCNNNNNNNGG 4 cut(s) 291, 1219, 1537, 1714
BseMI GCAATG 2 cut(s) 858, 1433
BseMII CTCAG 3 cut(s) 52, 839, 1218
BseRI GAGGAG 5 cut(s) 65, 68, 71, 139, 260
BseX3I CGGCCG 2 cut(s) 224, 372
BseXI GCAGC 6 cut(s) 222, 293, 458, 529, 1595, 1708
Bsh1285I CGRYCG 4 cut(s) 227, 231, 375, 559
BshFI GGCC 8 cut(s) 226, 272, 278, 365, 374, 696, 927, 1668
BshNI GGYRCC 1 cut(s) 476
BsiEI CGRYCG 4 cut(s) 227, 231, 375, 559
BsiHKAI GWGCWC 2 cut(s) 1152, 1506
BsiHKCI CYCGRG 2 cut(s) 266, 1212
BsiSI CCGG 8 cut(s) 139, 164, 223, 291, 306, 366, 465, 581
BslFI GGGAC 5 cut(s) 308, 1445, 1665, 1717, 1766
BslI CCNNNNNNNGG 4 cut(s) 291, 1219, 1537, 1714
BsmAI GTCTC 4 cut(s) 506, 828, 1056, 1195
BsmBI CGTCTC 1 cut(s) 506
BsmFI GGGAC 5 cut(s) 308, 1445, 1665, 1717, 1766
BsmI GAATGC 2 cut(s) 238, 747
BsnI GGCC 8 cut(s) 226, 272, 278, 365, 374, 696, 927, 1668
BsoBI CYCGRG 2 cut(s) 266, 1212
Bsp119I TTCGAA 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 1152, 1506
Bsp19I CCATGG 1 cut(s) 1669
BspANI GGCC 8 cut(s) 226, 272, 278, 365, 374, 696, 927, 1668
BspCNI CTCAG 3 cut(s) 51, 838, 1219
BspLI GGNNCC 3 cut(s) 478, 790, 1279
BspPI GGATC 4 cut(s) 698, 727, 783, 796
BspT104I TTCGAA 1 cut(s) 128
BspT107I GGYRCC 1 cut(s) 476
BsrDI GCAATG 2 cut(s) 858, 1433
BsrFI RCCGGY 1 cut(s) 464
BssAI RCCGGY 1 cut(s) 464
BssNI GRCGYC 3 cut(s) 297, 462, 477
BssT1I CCWWGG 3 cut(s) 153, 783, 1669
Bst2UI CCWGG 3 cut(s) 275, 1284, 1749
Bst4CI ACNGT 6 cut(s) 118, 194, 436, 949, 1056, 1614
Bst6I CTCTTC 1 cut(s) 1676
BstACI GRCGYC 3 cut(s) 297, 462, 477
BstAPI GCANNNNNTGC 1 cut(s) 1156
BstBI TTCGAA 1 cut(s) 128
BstC8I GCNNGC 3 cut(s) 698, 810, 1603
BstDEI CTNAG 4 cut(s) 38, 825, 1227, 1368
BstDSI CCRYGG 2 cut(s) 593, 1669
BstF5I GGATG 2 cut(s) 1627, 1679
BstH2I RGCGCY 5 cut(s) 161, 253, 480, 703, 716
BstHHI GCGC 6 cut(s) 160, 252, 311, 479, 702, 715
BstMAI GTCTC 4 cut(s) 506, 828, 1056, 1195
BstMCI CGRYCG 4 cut(s) 227, 231, 375, 559
BstNI CCWGG 3 cut(s) 275, 1284, 1749
BstNSI RCATGY 2 cut(s) 656, 1520
BstPAI GACNNNNGTC 1 cut(s) 1657
BstSCI CCNGG 6 cut(s) 273, 290, 304, 365, 1282, 1747
BstV1I GCAGC 6 cut(s) 222, 293, 458, 529, 1595, 1708
BstV2I GAAGAC 2 cut(s) 608, 874
BstX2I RGATCY 2 cut(s) 788, 979
BstXI CCANNNNNNTGG 1 cut(s) 841
BstYI RGATCY 2 cut(s) 788, 979
BstZI CGGCCG 2 cut(s) 224, 372
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 8 cut(s) 226, 272, 278, 365, 374, 696, 927, 1668
BtgI CCRYGG 2 cut(s) 593, 1669
BtgZI GCGATG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 217
BtsCI GGATG 2 cut(s) 1627, 1679
BtsIMutI CAGTG 3 cut(s) 20, 1052, 1610
Cac8I GCNNGC 3 cut(s) 698, 810, 1603
CfoI GCGC 6 cut(s) 160, 252, 311, 479, 702, 715
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 2 cut(s) 271, 802
CseI GACGC 5 cut(s) 107, 438, 470, 523, 1411
Csp6I GTAC 4 cut(s) 878, 997, 1007, 1691
CspCI CAANNNNNGTGG 2 cut(s) 575, 610
CviAII CATG 5 cut(s) 653, 1517, 1670, 1678, 1729
CviQI GTAC 4 cut(s) 878, 997, 1007, 1691
DdeI CTNAG 4 cut(s) 38, 825, 1227, 1368
DinI GGCGCC 1 cut(s) 478
DraI TTTAAA 1 cut(s) 1548
DraIII CACNNNGTG 1 cut(s) 1156
EaeI YGGCCR 4 cut(s) 224, 363, 372, 694
EagI CGGCCG 2 cut(s) 224, 372
Eam1104I CTCTTC 1 cut(s) 1676
EarI CTCTTC 1 cut(s) 1676
EciI GGCGGA 4 cut(s) 182, 196, 455, 560
EclXI CGGCCG 2 cut(s) 224, 372
Eco130I CCWWGG 3 cut(s) 153, 783, 1669
Eco32I GATATC 3 cut(s) 640, 678, 1485
Eco47I GGWCC 1 cut(s) 802
Eco47III AGCGCT 2 cut(s) 701, 714
Eco52I CGGCCG 2 cut(s) 224, 372
Eco57I CTGAAG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 266, 1212
EcoRI GAATTC 1 cut(s) 1327
EcoRII CCWGG 3 cut(s) 273, 1282, 1747
EcoRV GATATC 3 cut(s) 640, 678, 1485
EcoT14I CCWWGG 3 cut(s) 153, 783, 1669
EcoT22I ATGCAT 1 cut(s) 1679
EgeI GGCGCC 1 cut(s) 478
EheI GGCGCC 1 cut(s) 478
ErhI CCWWGG 3 cut(s) 153, 783, 1669
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 5 cut(s) 656, 1520, 1673, 1681, 1732
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 5 cut(s) 308, 1445, 1665, 1717, 1766
FatI CATG 5 cut(s) 652, 1516, 1669, 1677, 1728
FauI CCCGC 2 cut(s) 1579, 1610
Fnu4HI GCNGC 9 cut(s) 236, 279, 282, 372, 447, 518, 570, 1584, 1722
FokI GGATG 2 cut(s) 1634, 1686
Fsp4HI GCNGC 9 cut(s) 236, 279, 282, 372, 447, 518, 570, 1584, 1722
FspBI CTAG 1 cut(s) 974
GlaI GCGC 6 cut(s) 159, 251, 310, 478, 701, 714
GluI GCNGC 9 cut(s) 236, 279, 282, 372, 447, 518, 570, 1584, 1722
HaeII RGCGCY 5 cut(s) 161, 253, 480, 703, 716
HaeIII GGCC 8 cut(s) 226, 272, 278, 365, 374, 696, 927, 1668
HapII CCGG 8 cut(s) 139, 164, 223, 291, 306, 366, 465, 581
HgaI GACGC 5 cut(s) 107, 438, 470, 523, 1411
HhaI GCGC 6 cut(s) 160, 252, 311, 479, 702, 715
Hin1I GRCGYC 3 cut(s) 297, 462, 477
Hin1II CATG 5 cut(s) 656, 1520, 1673, 1681, 1732
Hin6I GCGC 6 cut(s) 158, 250, 309, 477, 700, 713
HinP1I GCGC 6 cut(s) 158, 250, 309, 477, 700, 713
HindIII AAGCTT 1 cut(s) 935
HinfI GANTC 3 cut(s) 170, 614, 1188
HpaII CCGG 8 cut(s) 139, 164, 223, 291, 306, 366, 465, 581
HphI GGTGA 7 cut(s) 76, 356, 368, 403, 412, 442, 479
Hpy166II GTNNAC 2 cut(s) 670, 890
Hpy188III TCNNGA 6 cut(s) 266, 1180, 1445, 1454, 1474, 1526
Hpy8I GTNNAC 2 cut(s) 670, 890
Hpy99I CGWCG 9 cut(s) 101, 194, 218, 258, 302, 338, 380, 454, 867
HpyAV CCTTC 6 cut(s) 391, 421, 917, 1215, 1492, 1612
HpyCH4III ACNGT 6 cut(s) 118, 194, 436, 949, 1056, 1614
HpyCH4IV ACGT 3 cut(s) 216, 297, 605
HpyCH4V TGCA 8 cut(s) 238, 764, 962, 1075, 1460, 1625, 1638, 1677
HpyF3I CTNAG 4 cut(s) 38, 825, 1227, 1368
HpySE526I ACGT 3 cut(s) 216, 297, 605
Hsp92I GRCGYC 3 cut(s) 297, 462, 477
Hsp92II CATG 5 cut(s) 656, 1520, 1673, 1681, 1732
HspAI GCGC 6 cut(s) 158, 250, 309, 477, 700, 713
KasI GGCGCC 1 cut(s) 476
LmnI GCTCC 3 cut(s) 247, 1147, 1501
Lsp1109I GCAGC 6 cut(s) 222, 293, 458, 529, 1595, 1708
LweI GCATC 5 cut(s) 320, 821, 1447, 1612, 1664
MaeI CTAG 1 cut(s) 974
MaeII ACGT 3 cut(s) 216, 297, 605
MaeIII GTNAC 3 cut(s) 430, 1306, 1418
MflI RGATCY 2 cut(s) 788, 979
MhlI GDGCHC 2 cut(s) 1152, 1506
MluCI AATT 7 cut(s) 33, 587, 1010, 1068, 1089, 1327, 1763
Mly113I GGCGCC 1 cut(s) 477
MlyI GAGTC 2 cut(s) 164, 1197
MmeI TCCRAC 5 cut(s) 198, 236, 237, 720, 1274
Mph1103I ATGCAT 1 cut(s) 1679
MroXI GAANNNNTTC 2 cut(s) 33, 605
MseI TTAA 9 cut(s) 1067, 1124, 1218, 1335, 1344, 1512, 1547, 1758, 1779
MslI CAYNNNNRTG 2 cut(s) 192, 839
MspA1I CMGCKG 1 cut(s) 1586
MspI CCGG 8 cut(s) 139, 164, 223, 291, 306, 366, 465, 581
MspR9I CCNGG 6 cut(s) 275, 292, 306, 367, 1284, 1749
Mva1269I GAATGC 2 cut(s) 238, 747
MvaI CCWGG 3 cut(s) 275, 1284, 1749
NarI GGCGCC 1 cut(s) 477
NciI CCSGG 3 cut(s) 292, 306, 367
NcoI CCATGG 1 cut(s) 1669
NlaIII CATG 5 cut(s) 656, 1520, 1673, 1681, 1732
NlaIV GGNNCC 3 cut(s) 478, 790, 1279
NmeAIII GCCGAG 1 cut(s) 453
NmuCI GTSAC 2 cut(s) 430, 1306
NsiI ATGCAT 1 cut(s) 1679
NspI RCATGY 2 cut(s) 656, 1520
NspV TTCGAA 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 192
PaeR7I CTCGAG 1 cut(s) 266
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 384
PctI GAATGC 2 cut(s) 238, 747
PdmI GAANNNNTTC 2 cut(s) 33, 605
PfeI GAWTC 1 cut(s) 614
PflFI GACNNNGTC 1 cut(s) 194
PkrI GCNGC 9 cut(s) 237, 280, 283, 373, 448, 519, 571, 1585, 1723
Ple19I CGATCG 1 cut(s) 231
PleI GAGTC 2 cut(s) 164, 1196
PluTI GGCGCC 1 cut(s) 480
PpsI GAGTC 2 cut(s) 164, 1196
PshAI GACNNNNGTC 1 cut(s) 1657
Psp6I CCWGG 3 cut(s) 273, 1282, 1747
PspGI CCWGG 3 cut(s) 273, 1282, 1747
PspN4I GGNNCC 3 cut(s) 478, 790, 1279
PspPI GGNCC 2 cut(s) 271, 802
PsuI RGATCY 2 cut(s) 788, 979
PsyI GACNNNGTC 1 cut(s) 194
PvuI CGATCG 1 cut(s) 231
RsaI GTAC 4 cut(s) 879, 998, 1008, 1692
RsaNI GTAC 4 cut(s) 878, 997, 1007, 1691
RseI CAYNNNNRTG 2 cut(s) 192, 839
SaqAI TTAA 9 cut(s) 1067, 1124, 1218, 1335, 1344, 1512, 1547, 1758, 1779
SatI GCNGC 9 cut(s) 236, 279, 282, 372, 447, 518, 570, 1584, 1722
Sau96I GGNCC 2 cut(s) 271, 802
ScaI AGTACT 1 cut(s) 879
SchI GAGTC 2 cut(s) 164, 1197
ScrFI CCNGG 6 cut(s) 275, 292, 306, 367, 1284, 1749
SduI GDGCHC 2 cut(s) 1152, 1506
SfaNI GCATC 5 cut(s) 320, 821, 1447, 1612, 1664
SfiI GGCCNNNNNGGCC 1 cut(s) 371
SfoI GGCGCC 1 cut(s) 478
Sfr274I CTCGAG 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 128
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 1 cut(s) 802
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 2 cut(s) 192, 839
SmlI CTYRAG 2 cut(s) 266, 1534
SmoI CTYRAG 2 cut(s) 266, 1534
Sse9I AATT 7 cut(s) 33, 587, 1010, 1068, 1089, 1327, 1763
SspDI GGCGCC 1 cut(s) 476
SspMI CTAG 1 cut(s) 974
StyD4I CCNGG 6 cut(s) 273, 290, 304, 365, 1282, 1747
StyI CCWWGG 3 cut(s) 153, 783, 1669
TaaI ACNGT 6 cut(s) 118, 194, 436, 949, 1056, 1614
TaiI ACGT 3 cut(s) 219, 300, 608
TaqI TCGA 9 cut(s) 27, 128, 231, 267, 314, 378, 452, 559, 1455
TaqII GACCGA 1 cut(s) 545
TasI AATT 7 cut(s) 33, 587, 1010, 1068, 1089, 1327, 1763
TatI WGTACW 2 cut(s) 877, 996
TauI GCSGC 3 cut(s) 281, 374, 572
TfiI GAWTC 1 cut(s) 614
Tru1I TTAA 9 cut(s) 1067, 1124, 1218, 1335, 1344, 1512, 1547, 1758, 1779
Tru9I TTAA 9 cut(s) 1067, 1124, 1218, 1335, 1344, 1512, 1547, 1758, 1779
TscAI CASTG 3 cut(s) 27, 1059, 1617
TseFI GTSAC 2 cut(s) 430, 1306
TseI GCWGC 6 cut(s) 235, 281, 446, 517, 1583, 1721
Tsp45I GTSAC 2 cut(s) 430, 1306
TspDTI ATGAA 2 cut(s) 936, 1694
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 3 cut(s) 27, 1059, 1617
Tth111I GACNNNGTC 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 802
XapI RAATTY 3 cut(s) 1089, 1327, 1763
XceI RCATGY 2 cut(s) 656, 1520
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XhoI CTCGAG 1 cut(s) 266
XmnI GAANNNNTTC 2 cut(s) 33, 605
XspI CTAG 1 cut(s) 974
ZraI GACGTC 1 cut(s) 298
ZrmI AGTACT 1 cut(s) 879
Zsp2I ATGCAT 1 cut(s) 1679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.