MD13G1022000.v1.1

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002090
Physical Location & Seq
Reverse (-)
1574994 .. 1578922
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1022000.v1.1.491

Sequence Viewer

Length: 1830 bp
ATGGAAGAAGATCAAGCTGCAGCTTTCGAATCAATCGGGAACGGCGACCATTACGACGCCGCATCCACCGCCGTCAATGATTACAGTACCCATCATACCACCACCAACAACGACCACGGTTGGCAGAAGGTCACCGCGAAGCGCCCGAGGAAGAAGAAGGAATCGAACGCGGAAACAATTAACAATCAGAACAAGCTGGTCCCCGGCGTCGCGAACGCCAACGGAAACGGCGTTTTCCGATCGCTGGAGAAACAGTCGGAGGACCGGCGCCGGCGGATTCTCGAAGCTCAGAGGGCCAATGTCGACCTTGACGATGTTGCTCCGGCGAGATCGAAGCTCAGGTCTGACGACGAGGATGGGGATAATAGCGATGACGAAGCTGCGGCGCAGAACGTGAAGACCGAGGAGGCGAAGAAATCCAAGCCTAAGAAGCCGAAGAAGCCTAAGGTCACGGTGGCTGAGGCGGCCGCTAAGATCGACGAGACAAACGATCTCGAGGCATTCCTCATTGACATTTCGGCTTCATACGAGTCGAAGGAGGACATACAGCTGATGCGTTTTGCGGACTATTTCGGCCGCGCATTCTCTGCGGTGTCTGCCTCTCAGTTTCCATGGGTGAAGATGTTCAAGGAGTCATCGGTGGCCAAGCTTGCCGATATCCCCCTTTCTCATATTTCTGAAACTGTTTATAAGACATCAGTTGACTGGATCAACCAGCGTTCCATTGAGGCCCTTGGGTCTTTTATATTATGGTCCTTAGAAAGCATTCTTGCAGACCTAGCGAGCCAAGTTTCTGGCACGAAGGGCAAGAAGGCTGTGCAGAATGTGTCTTCAAAATCTCAGGTTGCAATATTTGTGGTTGTAGCAATGGTATTGCGTAGAAGACCCGATGTCTTGATTAGTATATTGCCAGCACTGAGAGAAAATTCAAAGTATCAAGGACAAGATAAGCTTCCAGTAACTATATGGATGATAGTTCAAGCTAGTCAAGGGGACCTCGCTGTTGCGTTGCATGCATGGGCACGCAATGTGTTGCCTTTAGTCTGTGGAAAAAGTAGTAATCCACAGTCCCGAGACCTTGTTTTGCAGCTAGTGGAGAGAATTCTGTCTATGCCCAAAGCTCGTACTATTTTAGTAAATAGTGCTGTTAGGAAGGGGGAGCGTTTGGTGCCACCTTCTGCATTGGAGATACTTATGGGAGTTACCTTTCCTGCACATTCAGCTAGAGTAAAGGCCACCGAAAGGTTTGAGGCTATATATCCAACCCTAAAAGCGGTCGCTCTAGCTGGCGCCCCTGGAAGCAAAGCAATGAAACAAGTTTCACAGCAGATGTTGAGTTTTGCTGTCAAAGCTGCAGGAGAAAGTATACCAGAGCTATCCGATGAAGCAGCTGGAATTTTCATTTGGTGTTTGACCCAACATGCTGATTGCTTTAAGCAGTGGGACAAGGTTTATGAGGAAAACTTAGAAGCGAGTGTTGCTGTTCTGAAGAAACTTTCTAACCAATGGAAAGAACATTCTGCAAATCTGTCTCCTTTTGATCCTATGAGGGAAGCTCTTAAAAGTTTTAGTCGCAAGAATGAGAAGGCATTGGCTGGTGAGGCAGAGGATGCACGCCAAGAAAAGCTCAACAAGGAAGCAGACAAGTATTGTAAGACATTATTGGGGAAGATATCACGGGGAAGCGGGTGCAAGAAAACTGTGGCCCTTGCTGTTGTTGCCTTGGCTGTTGGTGCTGCTGTCATGTCCCCAAACGTAGAGTCTTGGGAATCGGAGTTGAAAAAACTAACAGCGACTGTCAGCTTGACTATCAGCTCTTTCTTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

610

Amino Acids

66.86

Weight (kDa)

8.74

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 109 - 579 1.1e-18 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 690
AccB1I GGYRCC 3 cut(s) 267, 1168, 1289
AccI GTMKAC 2 cut(s) 303, 1366
AccII CGCG 4 cut(s) 137, 170, 212, 579
AclWI GGATC 2 cut(s) 716, 1535
AcoI YGGCCR 3 cut(s) 465, 574, 642
AcsI RAATTY 3 cut(s) 925, 1101, 1395
AcuI CTGAAG 1 cut(s) 1508
AcyI GRCGYC 4 cut(s) 57, 207, 268, 1290
AfaI GTAC 2 cut(s) 88, 1126
AfiI CCNNNNNNNGG 3 cut(s) 244, 1242, 1273
AgsI TTSAA 5 cut(s) 628, 834, 930, 980, 1780
AhdI GACNNNNNGTC 1 cut(s) 890
AjnI CCWGG 1 cut(s) 1294
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
Alw26I GTCTC 3 cut(s) 476, 1068, 1536
AlwI GGATC 2 cut(s) 716, 1535
AlwNI CAGNNNCTG 1 cut(s) 1796
Ama87I CYCGRG 3 cut(s) 145, 494, 1071
AoxI GGCC 7 cut(s) 294, 465, 574, 642, 729, 1233, 1704
ApeKI GCWGC 7 cut(s) 17, 20, 380, 1087, 1352, 1388, 1736
ApoI RAATTY 3 cut(s) 925, 1101, 1395
ArsI GACNNNNNNTTYG 4 cut(s) 326, 358, 1745, 1777
Asp700I GAANNNNTTC 2 cut(s) 623, 765
AspLEI GCGC 5 cut(s) 144, 270, 388, 581, 1292
AspS9I GGNCC 7 cut(s) 199, 262, 294, 730, 753, 994, 1705
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 3 cut(s) 124, 628, 1610
AsuII TTCGAA 2 cut(s) 27, 1824
AvaI CYCGRG 3 cut(s) 145, 494, 1071
AvaII GGWCC 4 cut(s) 199, 262, 753, 994
AxyI CCTNAGG 1 cut(s) 444
BaeGI GKGCMC 1 cut(s) 1024
BalI TGGCCA 1 cut(s) 644
BanI GGYRCC 3 cut(s) 267, 1168, 1289
BbsI GAAGAC 3 cut(s) 404, 822, 889
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 7 cut(s) 4, 32, 367, 1099, 1339, 1400, 1723
BccI CCATC 2 cut(s) 99, 350
BceAI ACGGC 3 cut(s) 56, 58, 244
BciT130I CCWGG 1 cut(s) 1296
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 476, 1068, 1536
BfaI CTAG 5 cut(s) 779, 984, 1091, 1224, 1283
BfmI CTRYAG 2 cut(s) 18, 1353
BfoI RGCGCY 3 cut(s) 145, 271, 1293
Bme1390I CCNGG 2 cut(s) 204, 1296
Bme18I GGWCC 4 cut(s) 199, 262, 753, 994
BmeRI GACNNNNNGTC 1 cut(s) 890
BmeT110I CYCGRG 3 cut(s) 145, 494, 1071
BmgT120I GGNCC 7 cut(s) 199, 262, 294, 730, 753, 994, 1705
BmiI GGNNCC 5 cut(s) 201, 269, 995, 1170, 1291
BmrFI CCNGG 2 cut(s) 204, 1296
BmsI GCATC 3 cut(s) 71, 543, 1600
BpiI GAAGAC 3 cut(s) 404, 822, 889
BplI GAGNNNNNCTC 2 cut(s) 1540, 1572
BpmI CTGGAG 1 cut(s) 266
Bpu10I CCTNAGC 2 cut(s) 338, 459
Bpu14I TTCGAA 2 cut(s) 27, 1824
BpuMI CCSGG 1 cut(s) 204
BsaHI GRCGYC 4 cut(s) 57, 207, 268, 1290
BsaI GGTCTC 1 cut(s) 1068
BsaJI CCNNGG 8 cut(s) 115, 146, 202, 402, 611, 733, 1294, 1722
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 3 cut(s) 244, 1242, 1273
Bse118I RCCGGY 2 cut(s) 264, 270
Bse1I ACTGG 2 cut(s) 710, 956
Bse21I CCTNAGG 1 cut(s) 444
Bse3DI GCAATG 3 cut(s) 873, 1033, 1314
BseBI CCWGG 1 cut(s) 1296
BseDI CCNNGG 8 cut(s) 115, 146, 202, 402, 611, 733, 1294, 1722
BseGI GGATG 4 cut(s) 62, 361, 975, 1615
BseLI CCNNNNNNNGG 3 cut(s) 244, 1242, 1273
BseMI GCAATG 3 cut(s) 873, 1033, 1314
BseMII CTCAG 6 cut(s) 302, 352, 450, 617, 854, 908
BseNI ACTGG 2 cut(s) 710, 956
BseRI GAGGAG 1 cut(s) 419
BseSI GKGCMC 1 cut(s) 1024
BseX3I CGGCCG 2 cut(s) 465, 574
BseXI GCAGC 7 cut(s) 4, 32, 367, 1099, 1339, 1400, 1723
BsgI GTGCAG 2 cut(s) 839, 1197
Bsh1236I CGCG 4 cut(s) 137, 170, 212, 579
Bsh1285I CGRYCG 4 cut(s) 242, 468, 577, 1278
BshFI GGCC 7 cut(s) 296, 467, 576, 644, 731, 1235, 1706
BshNI GGYRCC 3 cut(s) 267, 1168, 1289
BsiEI CGRYCG 4 cut(s) 242, 468, 577, 1278
BsiHKCI CYCGRG 3 cut(s) 145, 494, 1071
BsiSI CCGG 4 cut(s) 204, 265, 271, 323
BslFI GGGAC 5 cut(s) 185, 1007, 1054, 1457, 1732
BslI CCNNNNNNNGG 3 cut(s) 244, 1242, 1273
BsmAI GTCTC 3 cut(s) 476, 1068, 1536
BsmFI GGGAC 5 cut(s) 185, 1007, 1054, 1457, 1732
BsmI GAATGC 3 cut(s) 500, 581, 765
BsnI GGCC 7 cut(s) 296, 467, 576, 644, 731, 1235, 1706
Bso31I GGTCTC 1 cut(s) 1068
BsoBI CYCGRG 3 cut(s) 145, 494, 1071
Bsp119I TTCGAA 2 cut(s) 27, 1824
Bsp1286I GDGCHC 1 cut(s) 1024
Bsp143I GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
Bsp19I CCATGG 1 cut(s) 611
Bsp68I TCGCGA 1 cut(s) 212
BspANI GGCC 7 cut(s) 296, 467, 576, 644, 731, 1235, 1706
BspCNI CTCAG 6 cut(s) 301, 351, 451, 616, 853, 909
BspFNI CGCG 4 cut(s) 137, 170, 212, 579
BspLI GGNNCC 5 cut(s) 201, 269, 995, 1170, 1291
BspMAI CTGCAG 2 cut(s) 22, 1357
BspPI GGATC 2 cut(s) 716, 1535
BspT104I TTCGAA 2 cut(s) 27, 1824
BspT107I GGYRCC 3 cut(s) 267, 1168, 1289
BspTNI GGTCTC 1 cut(s) 1068
BsrDI GCAATG 3 cut(s) 873, 1033, 1314
BsrFI RCCGGY 2 cut(s) 264, 270
BsrI ACTGG 2 cut(s) 710, 956
BssAI RCCGGY 2 cut(s) 264, 270
BssECI CCNNGG 8 cut(s) 115, 146, 202, 402, 611, 733, 1294, 1722
BssMI GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
BssNAI GTATAC 1 cut(s) 1367
BssNI GRCGYC 4 cut(s) 57, 207, 268, 1290
BssT1I CCWWGG 3 cut(s) 611, 733, 1722
Bst1107I GTATAC 1 cut(s) 1367
Bst2UI CCWGG 1 cut(s) 1296
Bst4CI ACNGT 8 cut(s) 86, 119, 255, 454, 685, 1068, 1702, 1798
BstACI GRCGYC 4 cut(s) 57, 207, 268, 1290
BstAPI GCANNNNNTGC 2 cut(s) 587, 1610
BstBI TTCGAA 2 cut(s) 27, 1824
BstC8I GCNNGC 8 cut(s) 272, 651, 784, 912, 1014, 1024, 1288, 1615
BstDSI CCRYGG 2 cut(s) 115, 611
BstEII GGTNACC 1 cut(s) 130
BstF5I GGATG 4 cut(s) 62, 361, 975, 1615
BstFNI CGCG 4 cut(s) 137, 170, 212, 579
BstH2I RGCGCY 3 cut(s) 145, 271, 1293
BstHHI GCGC 5 cut(s) 144, 270, 388, 581, 1292
BstKTI GATC 7 cut(s) 13, 242, 332, 477, 493, 711, 1543
BstMAI GTCTC 3 cut(s) 476, 1068, 1536
BstMBI GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
BstMCI CGRYCG 4 cut(s) 242, 468, 577, 1278
BstNI CCWGG 1 cut(s) 1296
BstNSI RCATGY 2 cut(s) 1016, 1424
BstPI GGTNACC 1 cut(s) 130
BstSCI CCNGG 2 cut(s) 202, 1294
BstSFI CTRYAG 2 cut(s) 18, 1353
BstSLI GKGCMC 1 cut(s) 1024
BstUI CGCG 4 cut(s) 137, 170, 212, 579
BstV1I GCAGC 7 cut(s) 4, 32, 367, 1099, 1339, 1400, 1723
BstV2I GAAGAC 3 cut(s) 404, 822, 889
BstXI CCANNNNNNTGG 1 cut(s) 794
BstZ17I GTATAC 1 cut(s) 1367
BstZI CGGCCG 2 cut(s) 465, 574
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 7 cut(s) 296, 467, 576, 644, 731, 1235, 1706
BtgI CCRYGG 2 cut(s) 115, 611
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 4 cut(s) 62, 361, 975, 1615
BtsI GCAGTG 1 cut(s) 1445
BtsIMutI CAGTG 2 cut(s) 914, 1445
BtuMI TCGCGA 1 cut(s) 212
Cac8I GCNNGC 8 cut(s) 272, 651, 784, 912, 1014, 1024, 1288, 1615
CaiI CAGNNNCTG 1 cut(s) 1796
CciNI GCGGCCGC 1 cut(s) 465
CfoI GCGC 5 cut(s) 144, 270, 388, 581, 1292
Cfr10I RCCGGY 2 cut(s) 264, 270
Cfr13I GGNCC 7 cut(s) 199, 262, 294, 730, 753, 994, 1705
CseI GACGC 2 cut(s) 65, 196
Csp6I GTAC 2 cut(s) 87, 1125
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 5 cut(s) 612, 1013, 1017, 1421, 1744
CviQI GTAC 2 cut(s) 87, 1125
DinI GGCGCC 2 cut(s) 269, 1291
DpnI GATC 7 cut(s) 12, 241, 331, 476, 492, 710, 1542
DpnII GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
DriI GACNNNNNGTC 1 cut(s) 890
EaeI YGGCCR 3 cut(s) 465, 574, 642
EagI CGGCCG 2 cut(s) 465, 574
Eam1105I GACNNNNNGTC 1 cut(s) 890
EciI GGCGGA 1 cut(s) 289
EclXI CGGCCG 2 cut(s) 465, 574
Eco130I CCWWGG 3 cut(s) 611, 733, 1722
Eco31I GGTCTC 1 cut(s) 1068
Eco32I GATATC 2 cut(s) 658, 1674
Eco47I GGWCC 4 cut(s) 199, 262, 753, 994
Eco52I CGGCCG 2 cut(s) 465, 574
Eco57I CTGAAG 1 cut(s) 1508
Eco81I CCTNAGG 1 cut(s) 444
Eco88I CYCGRG 3 cut(s) 145, 494, 1071
Eco91I GGTNACC 1 cut(s) 130
EcoO109I RGGNCCY 2 cut(s) 730, 994
EcoO65I GGTNACC 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 1101
EcoRII CCWGG 1 cut(s) 1294
EcoRV GATATC 2 cut(s) 658, 1674
EcoT14I CCWWGG 3 cut(s) 611, 733, 1722
EcoT22I ATGCAT 1 cut(s) 1018
EgeI GGCGCC 2 cut(s) 269, 1291
EheI GGCGCC 2 cut(s) 269, 1291
ErhI CCWWGG 3 cut(s) 611, 733, 1722
FaeI CATG 5 cut(s) 615, 1016, 1020, 1424, 1747
FalI AAGNNNNNCTT 2 cut(s) 936, 968
FaqI GGGAC 5 cut(s) 185, 1007, 1054, 1457, 1732
FatI CATG 5 cut(s) 611, 1012, 1016, 1420, 1743
FauI CCCGC 1 cut(s) 1679
FblI GTMKAC 2 cut(s) 303, 1366
FokI GGATG 4 cut(s) 49, 368, 982, 1622
FspBI CTAG 5 cut(s) 779, 984, 1091, 1224, 1283
GlaI GCGC 5 cut(s) 143, 269, 387, 580, 1291
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 3 cut(s) 145, 271, 1293
HaeIII GGCC 7 cut(s) 296, 467, 576, 644, 731, 1235, 1706
HapII CCGG 4 cut(s) 204, 265, 271, 323
HgaI GACGC 2 cut(s) 65, 196
HhaI GCGC 5 cut(s) 144, 270, 388, 581, 1292
Hin1I GRCGYC 4 cut(s) 57, 207, 268, 1290
Hin1II CATG 5 cut(s) 615, 1016, 1020, 1424, 1747
Hin6I GCGC 5 cut(s) 142, 268, 386, 579, 1290
HinP1I GCGC 5 cut(s) 142, 268, 386, 579, 1290
HincII GTYRAC 2 cut(s) 304, 703
HindII GTYRAC 2 cut(s) 304, 703
HindIII AAGCTT 2 cut(s) 647, 950
HinfI GANTC 7 cut(s) 29, 161, 277, 530, 632, 1760, 1769
HpaII CCGG 4 cut(s) 204, 265, 271, 323
HphI GGTGA 3 cut(s) 124, 628, 1610
Hpy166II GTNNAC 3 cut(s) 304, 703, 1367
Hpy188I TCNGA 9 cut(s) 189, 239, 259, 291, 346, 679, 1381, 1488, 1774
Hpy188III TCNNGA 6 cut(s) 37, 211, 281, 494, 895, 1071
Hpy8I GTNNAC 3 cut(s) 304, 703, 1367
Hpy99I CGWCG 4 cut(s) 59, 212, 353, 482
HpyAV CCTTC 8 cut(s) 121, 151, 529, 796, 805, 1147, 1185, 1579
HpyCH4III ACNGT 8 cut(s) 86, 119, 255, 454, 685, 1068, 1702, 1798
HpyCH4IV ACGT 2 cut(s) 393, 1755
HpySE526I ACGT 2 cut(s) 393, 1755
Hsp92I GRCGYC 4 cut(s) 57, 207, 268, 1290
Hsp92II CATG 5 cut(s) 615, 1016, 1020, 1424, 1747
HspAI GCGC 5 cut(s) 142, 268, 386, 579, 1290
KasI GGCGCC 2 cut(s) 267, 1289
KroI GCCGGC 1 cut(s) 270
KroNI GCCGGC 1 cut(s) 272
Kzo9I GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
LmnI GCTCC 2 cut(s) 325, 1159
Lsp1109I GCAGC 7 cut(s) 4, 32, 367, 1099, 1339, 1400, 1723
LweI GCATC 3 cut(s) 71, 543, 1600
MaeI CTAG 5 cut(s) 779, 984, 1091, 1224, 1283
MaeII ACGT 2 cut(s) 393, 1755
MaeIII GTNAC 4 cut(s) 130, 448, 958, 1201
MalI GATC 7 cut(s) 12, 241, 331, 476, 492, 710, 1542
MboI GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
MhlI GDGCHC 1 cut(s) 1024
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 4 cut(s) 177, 925, 1101, 1395
MluNI TGGCCA 1 cut(s) 644
Mly113I GGCGCC 2 cut(s) 268, 1290
MlyI GAGTC 3 cut(s) 539, 641, 1769
MmeI TCCRAC 2 cut(s) 237, 1286
Mox20I TGGCCA 1 cut(s) 644
Mph1103I ATGCAT 1 cut(s) 1018
MreI CGCCGGCG 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 270
MroXI GAANNNNTTC 2 cut(s) 623, 765
MscI TGGCCA 1 cut(s) 644
MseI TTAA 3 cut(s) 180, 1434, 1560
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 550, 1391
MspI CCGG 4 cut(s) 204, 265, 271, 323
MspR9I CCNGG 2 cut(s) 204, 1296
Mva1269I GAATGC 3 cut(s) 500, 581, 765
MvaI CCWGG 1 cut(s) 1296
MvnI CGCG 4 cut(s) 137, 170, 212, 579
NaeI GCCGGC 1 cut(s) 272
NarI GGCGCC 2 cut(s) 268, 1290
NciI CCSGG 1 cut(s) 204
NcoI CCATGG 1 cut(s) 611
NdeII GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
NgoMIV GCCGGC 1 cut(s) 270
NlaIII CATG 5 cut(s) 615, 1016, 1020, 1424, 1747
NlaIV GGNNCC 5 cut(s) 201, 269, 995, 1170, 1291
NmuCI GTSAC 2 cut(s) 130, 448
NotI GCGGCCGC 1 cut(s) 465
NruI TCGCGA 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 2 cut(s) 1016, 1424
NspV TTCGAA 2 cut(s) 27, 1824
PaeI GCATGC 1 cut(s) 1016
PaeR7I CTCGAG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 5 cut(s) 42, 228, 309, 399, 486
PctI GAATGC 3 cut(s) 500, 581, 765
PdiI GCCGGC 1 cut(s) 272
PdmI GAANNNNTTC 2 cut(s) 623, 765
PfeI GAWTC 4 cut(s) 29, 161, 277, 1769
Ple19I CGATCG 1 cut(s) 242
PleI GAGTC 3 cut(s) 538, 640, 1768
PluTI GGCGCC 2 cut(s) 271, 1293
PpsI GAGTC 3 cut(s) 538, 640, 1768
PpuMI RGGWCCY 1 cut(s) 994
PsiI TTATAA 1 cut(s) 690
Psp5II RGGWCCY 1 cut(s) 994
Psp6I CCWGG 1 cut(s) 1294
PspEI GGTNACC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 1294
PspN4I GGNNCC 5 cut(s) 201, 269, 995, 1170, 1291
PspPI GGNCC 7 cut(s) 199, 262, 294, 730, 753, 994, 1705
PspPPI RGGWCCY 1 cut(s) 994
PstI CTGCAG 2 cut(s) 22, 1357
PstNI CAGNNNCTG 1 cut(s) 1796
PvuI CGATCG 1 cut(s) 242
PvuII CAGCTG 2 cut(s) 550, 1391
RruI TCGCGA 1 cut(s) 212
RsaI GTAC 2 cut(s) 88, 1126
RsaNI GTAC 2 cut(s) 87, 1125
SalI GTCGAC 1 cut(s) 302
SaqAI TTAA 3 cut(s) 180, 1434, 1560
Sau3AI GATC 7 cut(s) 10, 239, 329, 474, 490, 708, 1540
Sau96I GGNCC 7 cut(s) 199, 262, 294, 730, 753, 994, 1705
SchI GAGTC 3 cut(s) 539, 641, 1769
ScrFI CCNGG 2 cut(s) 204, 1296
SduI GDGCHC 1 cut(s) 1024
SfaNI GCATC 3 cut(s) 71, 543, 1600
SfcI CTRYAG 2 cut(s) 18, 1353
SfoI GGCGCC 2 cut(s) 269, 1291
Sfr274I CTCGAG 1 cut(s) 494
SfuI TTCGAA 2 cut(s) 27, 1824
SgrAI CRCCGGYG 1 cut(s) 270
SinI GGWCC 4 cut(s) 199, 262, 753, 994
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 4 cut(s) 177, 925, 1101, 1395
SspDI GGCGCC 2 cut(s) 267, 1289
SspI AATATT 1 cut(s) 852
SspMI CTAG 5 cut(s) 779, 984, 1091, 1224, 1283
StyD4I CCNGG 2 cut(s) 202, 1294
StyI CCWWGG 3 cut(s) 611, 733, 1722
TaaI ACNGT 8 cut(s) 86, 119, 255, 454, 685, 1068, 1702, 1798
TaiI ACGT 2 cut(s) 396, 1758
TaqI TCGA 9 cut(s) 27, 164, 282, 303, 332, 477, 495, 533, 1824
TaqII GACCGA 1 cut(s) 416
TasI AATT 4 cut(s) 177, 925, 1101, 1395
TauI GCSGC 5 cut(s) 62, 386, 467, 470, 579
TfiI GAWTC 4 cut(s) 29, 161, 277, 1769
Tru1I TTAA 3 cut(s) 180, 1434, 1560
Tru9I TTAA 3 cut(s) 180, 1434, 1560
TscAI CASTG 2 cut(s) 921, 1445
TseFI GTSAC 2 cut(s) 130, 448
TseI GCWGC 7 cut(s) 17, 20, 380, 1087, 1352, 1388, 1736
Tsp45I GTSAC 2 cut(s) 130, 448
TspDTI ATGAA 4 cut(s) 513, 1325, 1390, 1398
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 2 cut(s) 921, 1445
VpaK11BI GGWCC 4 cut(s) 199, 262, 753, 994
XapI RAATTY 3 cut(s) 925, 1101, 1395
XceI RCATGY 2 cut(s) 1016, 1424
XcmI CCANNNNNNNNNTGG 1 cut(s) 963
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 303, 1366
XmnI GAANNNNTTC 2 cut(s) 623, 765
XspI CTAG 5 cut(s) 779, 984, 1091, 1224, 1283
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.