AT1G28370

DNA-binding domain in plant proteins such as APETALA2 and EREBPs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004034
Physical Location & Seq
Reverse (-)
9955774 .. 9956980
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G28370.1

Sequence Viewer

Length: 501 bp
ATGGCACCGACAGTTAAAACGGCGGCCGTCAAAACCAACGAAGGTAACGGAGTCCGTTACAGAGGAGTGAGGAAGAGACCATGGGGACGTTACGCAGCCGAGATCAGAGATCCTTTCAAGAAGTCACGTGTCTGGCTCGGTACTTTCGACACTCCTGAAGAAGCCGCTCGTGCCTACGACAAACGTGCTATTGAGTTTCGTGGAGCTAAAGCCAAAACCAACTTCCCTTGTTACAACATCAACGCCCACTGCTTGAGTTTGACACAGAGCCTGAGCCAGAGCAGCACCGTGGAATCATCGTTTCCTAATCTCAACCTCGGATCTGACTCTGTTAGTTCGAGATTCCCTTTTCCTAAGATTCAGGTTAAGGCTGGGATGATGGTGTTCGATGAAAGGAGTGAATCGGATTCTTCGTCGGTGGTGATGGATGTCGTTAGATATGAAGGACGACGTGTGGTTTTGGACTTGGATCTTAATTTCCCTCCTCCACCTGAGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

166

Amino Acids

18.61

Weight (kDa)

9.48

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 19 - 68 6.7e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 2 cut(s) 23, 165
AclWI GGATC 3 cut(s) 104, 328, 477
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 177
AcvI CACGTG 1 cut(s) 128
AfaI GTAC 1 cut(s) 142
AflIII ACRYGT 2 cut(s) 127, 451
AgsI TTSAA 1 cut(s) 118
AjiI CACGTC 1 cut(s) 452
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 3 cut(s) 104, 328, 477
AlwNI CAGNNNCTG 1 cut(s) 271
AoxI GGCC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 95, 282
AsuHPI GGTGA 1 cut(s) 433
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 168
BbrPI CACGTG 1 cut(s) 128
BbvI GCAGC 2 cut(s) 107, 294
BccI CCATC 2 cut(s) 373, 418
BceAI ACGGC 2 cut(s) 11, 36
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BcoDI GTCTC 1 cut(s) 70
BisI GCNGC 4 cut(s) 24, 96, 165, 283
BlsI GCNGC 4 cut(s) 25, 97, 166, 284
BmgBI CACGTC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 6
Bpu10I CCTNAGC 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 274
BsaAI YACGTR 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 70
BsaJI CCNNGG 3 cut(s) 80, 288, 316
BseDI CCNNGG 3 cut(s) 80, 288, 316
BseGI GGATG 2 cut(s) 381, 433
BseMII CTCAG 2 cut(s) 263, 483
BseRI GAGGAG 2 cut(s) 78, 474
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 107, 294
BseYI CCCAGC 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 99
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 1 cut(s) 26
Bso31I GGTCTC 1 cut(s) 70
Bsp143I GATC 4 cut(s) 102, 109, 320, 469
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 2 cut(s) 23, 165
BspANI GGCC 1 cut(s) 26
BspCNI CTCAG 2 cut(s) 264, 484
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 104, 328, 477
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 80, 288, 316
BssMI GATC 4 cut(s) 102, 109, 320, 469
BssSI CACGAG 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 80
Bst2BI CACGAG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 13, 289
Bst6I CTCTTC 1 cut(s) 68
BstBAI YACGTR 1 cut(s) 128
BstDEI CTNAG 3 cut(s) 272, 354, 492
BstDSI CCRYGG 2 cut(s) 80, 288
BstF5I GGATG 2 cut(s) 381, 433
BstKTI GATC 4 cut(s) 105, 112, 323, 472
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 4 cut(s) 102, 109, 320, 469
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 170, 282
BstV1I GCAGC 2 cut(s) 107, 294
BstX2I RGATCY 3 cut(s) 109, 320, 469
BstYI RGATCY 3 cut(s) 109, 320, 469
BstZI CGGCCG 1 cut(s) 24
BsuRI GGCC 1 cut(s) 26
BtgI CCRYGG 2 cut(s) 80, 288
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 2 cut(s) 381, 433
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 247
CaiI CAGNNNCTG 1 cut(s) 271
Csp6I GTAC 1 cut(s) 141
CviAII CATG 1 cut(s) 81
CviJI RGCY 9 cut(s) 26, 98, 136, 164, 206, 212, 270, 276, 371
CviKI_1 RGCY 9 cut(s) 26, 98, 136, 164, 206, 212, 270, 276, 371
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 3 cut(s) 272, 354, 492
DpnI GATC 4 cut(s) 104, 111, 322, 471
DpnII GATC 4 cut(s) 102, 109, 320, 469
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 80
Eco31I GGTCTC 1 cut(s) 70
Eco52I CGGCCG 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 177
Eco72I CACGTG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 1 cut(s) 84
FaiI YATR 2 cut(s) 82, 441
FaqI GGGAC 1 cut(s) 99
FatI CATG 1 cut(s) 80
Fnu4HI GCNGC 4 cut(s) 24, 96, 165, 283
FokI GGATG 2 cut(s) 388, 440
Fsp4HI GCNGC 4 cut(s) 24, 96, 165, 283
GluI GCNGC 4 cut(s) 24, 96, 165, 283
GsaI CCCAGC 1 cut(s) 375
HaeIII GGCC 1 cut(s) 26
Hin1II CATG 1 cut(s) 84
HinfI GANTC 7 cut(s) 51, 293, 326, 342, 358, 401, 407
HphI GGTGA 1 cut(s) 433
Hpy188I TCNGA 4 cut(s) 107, 320, 325, 406
Hpy188III TCNNGA 3 cut(s) 118, 155, 339
Hpy99I CGWCG 2 cut(s) 418, 453
HpyAV CCTTC 2 cut(s) 35, 437
HpyCH4III ACNGT 2 cut(s) 13, 289
HpyCH4IV ACGT 4 cut(s) 88, 127, 184, 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 282
HpyF3I CTNAG 3 cut(s) 272, 354, 492
HpySE526I ACGT 4 cut(s) 88, 127, 184, 451
Hsp92II CATG 1 cut(s) 84
Kzo9I GATC 4 cut(s) 102, 109, 320, 469
LmnI GCTCC 1 cut(s) 203
LpnPI CCDG 6 cut(s) 118, 168, 284, 290, 347, 357
Lsp1109I GCAGC 2 cut(s) 107, 294
MaeII ACGT 4 cut(s) 88, 127, 184, 451
MaeIII GTNAC 5 cut(s) 44, 56, 89, 123, 230
MalI GATC 4 cut(s) 104, 111, 322, 471
MbiI CCGCTC 1 cut(s) 167
MboI GATC 4 cut(s) 102, 109, 320, 469
MboII GAAGA 3 cut(s) 85, 170, 402
MflI RGATCY 3 cut(s) 109, 320, 469
MluCI AATT 1 cut(s) 475
MlyI GAGTC 2 cut(s) 60, 320
MnlI CCTC 5 cut(s) 56, 63, 326, 492, 495
MseI TTAA 3 cut(s) 15, 366, 474
MwoI GCNNNNNNNGC 2 cut(s) 170, 282
NcoI CCATGG 1 cut(s) 80
NdeII GATC 4 cut(s) 102, 109, 320, 469
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 410
PfeI GAWTC 5 cut(s) 293, 342, 358, 401, 407
PkrI GCNGC 4 cut(s) 25, 97, 166, 284
PleI GAGTC 2 cut(s) 59, 320
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 2 cut(s) 59, 320
Ppu21I YACGTR 1 cut(s) 128
PspCI CACGTG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 3 cut(s) 109, 320, 469
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
SaqAI TTAA 3 cut(s) 15, 366, 474
SatI GCNGC 4 cut(s) 24, 96, 165, 283
Sau3AI GATC 4 cut(s) 102, 109, 320, 469
SchI GAGTC 2 cut(s) 60, 320
SetI ASST 9 cut(s) 46, 91, 130, 187, 208, 318, 366, 454, 493
SmlI CTYRAG 1 cut(s) 253
SmoI CTYRAG 1 cut(s) 253
Sse9I AATT 1 cut(s) 475
SsiI CCGC 2 cut(s) 23, 165
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 13, 289
TaiI ACGT 4 cut(s) 91, 130, 187, 454
TaqI TCGA 3 cut(s) 147, 338, 387
TasI AATT 1 cut(s) 475
TauI GCSGC 2 cut(s) 26, 167
TfiI GAWTC 5 cut(s) 293, 342, 358, 401, 407
Tru1I TTAA 3 cut(s) 15, 366, 474
Tru9I TTAA 3 cut(s) 15, 366, 474
TscAI CASTG 1 cut(s) 254
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 2 cut(s) 95, 282
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 2 cut(s) 405, 456
TspGWI ACGGA 2 cut(s) 44, 63
TspRI CASTG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.