Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0004034

Physical Location & Seq

Genomic Coordinates Reverse (-)

21896844 .. 21901974

Gene Structure 5131 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0224700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1629 bp

ATGGGCACTCTCTCGCATGCGATCGCGCCCAGGCTCGCCGCCCTCCACCACCGCCGCCGTCACGGTAATGGAGTATTCTCCTCCTCCTCCGTCTCTTCTTCTTCTCGAACCTCGCGCTTTTCTTCTTCTTCTGCTTCGGGATTGACGACGAAGAAGAAGAGCAGCAGCGTTCGAGTCATGGCCGGGATCAAGCACGACCACCGCGACCTCGATTTCAGCGATCCTGACTGGAAATCAAAGTTCCAGAGCGATTTCGAGAAGCGCTTTGCCATCCCTCACATCACCGACGTCTTCCCCGACGCCGTTTCCATTCCCTCCACCTTCTGCCTCAAGATGAGAACTCCGGTCATCGAAGACTTCGCCGCCGGATATCCCTCCGACGAGGAGTGGCACGGTTACATAAATAACAACGATCGCGTACTTCTCAAGACTATATACTACTCGTCACCTACGTCTGCTGGTGCCGAATGCATTGATCCTAACTGTACTTGGGTGGAGCAATGGGTTCATCGTGCTGGGCCACGAGAAAAGATATATTTTAAACCCGAAGAAGTGAAAGCAGCAATTGTAACATGCGGAGGGCTCTGTCCTGGTCTTAATGATGTCATCAGACAGATTGTCATTACACTTGAAATATATGGTGTAAAAAAGATTGTTGGTATTCCTTACGGTTATCGTGGCTTTTCTGATCCAGAATTAGCCGAAATGCCACTCTCAAGGAAGGTGGTTCAAAATGTTCATCTTTCTGGTGGAAGCTTGTTAGGAGTTTCACGTGGAGGCCCCAGCGTTAGTGAAATAGTGGACAATTTAGAGGAAAGAGGGATCAATATGCTCTTTGTTTTGGGTGGAAACGGCACACATGCTGGAGCCGATGCAATACACACTGAGTGCCGCAAAAGACAGTTAAGGGTGGCTATAGTTGGTGTGCCGAAAACCATAGACAATGACATTTTGTTAATGGACAAAACATTTGGTTTCGATACTGCTGTTGAAGAAGCCCAGCGAGCAATAAATTCAGCATATATTGAGGCACATAGTGCTTACCATGGTATTGGCATTGTGAAATTGATGGGTCGTAGCAGTGGATTCATAGCAATGCACGCATCCCTGGCTAGTGGACAAATTGACATATGTTTGATACCTGAGGTACCTTTTCAAATACATGGCCCTCATGGTATTCTGCACCATCTGAAATACCTGATTGAAACAAAGGGATCAGCTGTGGTCTGTGTAGCAGAGGGAGCAGGGCAGAATTTACTACAGAAAACCAATGCTACTGATGCATCAGGAAATGTTGTATATGGGGATATTGGAGTACATATTCAACAAGAGACAAAGAAACATTTTAAGGAGCATGGTGTTCCTGTTGATGTGAAATATATAGACCCCACATACATGATTCGTGCATGTCGTGCAAATGCATCAGATGGAATTCTTTGCGCTGTGCTAGGGCAGAATGCCGTTCATGGTGCATTTGCTGGATATAGTGGCATCACAATTGGCATATGCAACACTCATTATGTATATCTTCCGATTCCTGAGGTCATTTCTCATCCCCGACTGGTGGATCCCAACAGTCGAATGTGGCACCGTTGCTTGACTTCAACAGGCCAGCCAGACTTCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003700 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AP2

Protein Analysis

542

Amino Acids

59.12

Weight (kDa)

7.66

Isoelectric Point (pI)

36.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	187 - 489	8.9e-62	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12980 AT1G28360 AT1G28370 AT1G28370 AT5G44210
fragaria_vesca	FvH4_3g08790 FvH4_7g26410
malus_domestica	MD05G1311600.v1.1 MD10G1290900.v1.1
prunus_persica	Prupe.4G051200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0008971
rosa_laevigata	RLG00000031621
rosa_multiflora	Rmu_sc0001875.1_g000010 Rmu_sc0004677.1_g000023 Rmu_ssc0000183.1_g000016
rosa_roxburghii	Rroxscaffold_1G00067050 Rroxscaffold_1G00067680
rosa_rugosa	Rorug02G0224700
rosa_samantha	Rh2AG229600 Rh2CG100300 Rh2CG233700 Rh2DG237300 Rh3BG166700 Rh3DG166100 Rh4BG243800 Rh5AG069400 Rh5BG066200 Rh5CG077400 Rh5DG065400 Rh7AG201600
rosa_wichuraiana	Rw1G005600 Rw5G006480 Rw7G023460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	291
Acc65I	GGTACC	1 cut(s)	1147
AccB1I	GGYRCC	3 cut(s)	461, 1147, 1587
AccII	CGCG	4 cut(s)	26, 115, 204, 417
AciI	CCGC	7 cut(s)	39, 52, 55, 202, 363, 576, 892
AclWI	GGATC	8 cut(s)	194, 215, 470, 683, 830, 1222, 1562, 1575
AcoI	YGGCCR	1 cut(s)	180
AcsI	RAATTY	3 cut(s)	1012, 1252, 1431
AcvI	CACGTG	1 cut(s)	773
AcyI	GRCGYC	2 cut(s)	288, 300
AdeI	CACNNNGTG	2 cut(s)	888, 1037
AfaI	GTAC	4 cut(s)	420, 487, 1149, 1317
AfeI	AGCGCT	1 cut(s)	263
AfiI	CCNNNNNNNGG	1 cut(s)	1564
AgsI	TTSAA	7 cut(s)	632, 731, 992, 1157, 1205, 1325, 1605
AhdI	GACNNNNNGTC	1 cut(s)	617
AjnI	CCWGG	3 cut(s)	29, 589, 1107
AluBI	AGCT	2 cut(s)	756, 1220
AluI	AGCT	2 cut(s)	756, 1220
Alw26I	GTCTC	2 cut(s)	97, 1325
AlwI	GGATC	8 cut(s)	194, 215, 470, 683, 830, 1222, 1562, 1575
Aor51HI	AGCGCT	1 cut(s)	263
AoxI	GGCC	5 cut(s)	180, 518, 778, 1165, 1609
ApeKI	GCWGC	3 cut(s)	162, 165, 560
ApoI	RAATTY	3 cut(s)	1012, 1252, 1431
ArsI	GACNNNNNNTTYG	2 cut(s)	953, 985
AsiSI	GCGATCGC	1 cut(s)	24
Asp718I	GGTACC	1 cut(s)	1147
AspLEI	GCGC	4 cut(s)	28, 117, 264, 1442
AspS9I	GGNCC	3 cut(s)	518, 779, 1166
AsuC2I	CCSGG	1 cut(s)	184
AsuHPI	GGTGA	2 cut(s)	274, 438
AxyI	CCTNAGG	2 cut(s)	1143, 1539
BaeGI	GKGCMC	1 cut(s)	8
BaeI	ACNNNNGTAYC	2 cut(s)	972, 1005
BamHI	GGATCC	1 cut(s)	1567
BanI	GGYRCC	3 cut(s)	461, 1147, 1587
BanII	GRGCYC	1 cut(s)	585
BauI	CACGAG	1 cut(s)	522
BbrPI	CACGTG	1 cut(s)	773
BbsI	GAAGAC	2 cut(s)	283, 360
BbvI	GCAGC	3 cut(s)	174, 177, 572
BccI	CCATC	4 cut(s)	278, 1063, 1194, 1421
BceAI	ACGGC	4 cut(s)	42, 287, 868, 1445
BciT130I	CCWGG	3 cut(s)	31, 591, 1109
BcnI	CCSGG	1 cut(s)	184
BcoDI	GTCTC	2 cut(s)	97, 1325
BfaI	CTAG	2 cut(s)	1113, 1448
BfmI	CTRYAG	2 cut(s)	915, 1259
BfoI	RGCGCY	1 cut(s)	265
BisI	GCNGC	7 cut(s)	39, 55, 163, 166, 363, 561, 892
BlsI	GCNGC	7 cut(s)	40, 56, 164, 167, 364, 562, 893
Bme1390I	CCNGG	4 cut(s)	31, 184, 591, 1109
BmeRI	GACNNNNNGTC	1 cut(s)	617
BmgT120I	GGNCC	3 cut(s)	518, 779, 1166
BmiI	GGNNCC	6 cut(s)	463, 781, 868, 1149, 1569, 1589
BmrFI	CCNGG	4 cut(s)	31, 184, 591, 1109
BmsI	GCATC	6 cut(s)	862, 1112, 1270, 1292, 1430, 1500
BpiI	GAAGAC	2 cut(s)	283, 360
BpmI	CTGGAG	1 cut(s)	885
BpuEI	CTTGAG	3 cut(s)	314, 410, 700
BpuMI	CCSGG	1 cut(s)	184
BsaAI	YACGTR	1 cut(s)	773
BsaHI	GRCGYC	2 cut(s)	288, 300
BsaJI	CCNNGG	3 cut(s)	29, 1045, 1107
BsaWI	WCCGGW	1 cut(s)	343
Bsc4I	CCNNNNNNNGG	1 cut(s)	1564
Bse1I	ACTGG	2 cut(s)	233, 1566
Bse21I	CCTNAGG	2 cut(s)	1143, 1539
Bse3DI	GCAATG	2 cut(s)	506, 1101
BseBI	CCWGG	3 cut(s)	31, 591, 1109
BseDI	CCNNGG	3 cut(s)	29, 1045, 1107
BseGI	GGATG	3 cut(s)	270, 1103, 1552
BseLI	CCNNNNNNNGG	1 cut(s)	1564
BseMI	GCAATG	2 cut(s)	506, 1101
BseMII	CTCAG	3 cut(s)	876, 1134, 1530
BseNI	ACTGG	2 cut(s)	233, 1566
BseRI	GAGGAG	4 cut(s)	70, 73, 76, 398
BseSI	GKGCMC	1 cut(s)	8
BseXI	GCAGC	3 cut(s)	174, 177, 572
BseYI	CCCAGC	3 cut(s)	515, 782, 999
BsgI	GTGCAG	1 cut(s)	1166
Bsh1236I	CGCG	4 cut(s)	26, 115, 204, 417
Bsh1285I	CGRYCG	2 cut(s)	24, 415
BshFI	GGCC	5 cut(s)	182, 520, 780, 1167, 1611
BshNI	GGYRCC	3 cut(s)	461, 1147, 1587
BsiEI	CGRYCG	2 cut(s)	24, 415
BsiSI	CCGG	3 cut(s)	183, 344, 366
BslI	CCNNNNNNNGG	1 cut(s)	1564
BsmAI	GTCTC	2 cut(s)	97, 1325
BsmBI	CGTCTC	1 cut(s)	97
BsmI	GAATGC	2 cut(s)	473, 1462
BsnI	GGCC	5 cut(s)	182, 520, 780, 1167, 1611
Bsp1286I	GDGCHC	2 cut(s)	8, 585
Bsp143I	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
Bsp19I	CCATGG	1 cut(s)	1045
BspACI	CCGC	7 cut(s)	39, 52, 55, 202, 363, 576, 892
BspANI	GGCC	5 cut(s)	182, 520, 780, 1167, 1611
BspCNI	CTCAG	3 cut(s)	877, 1135, 1531
BspFNI	CGCG	4 cut(s)	26, 115, 204, 417
BspLI	GGNNCC	6 cut(s)	463, 781, 868, 1149, 1569, 1589
BspPI	GGATC	8 cut(s)	194, 215, 470, 683, 830, 1222, 1562, 1575
BspQI	GCTCTTC	1 cut(s)	152
BspT107I	GGYRCC	3 cut(s)	461, 1147, 1587
BsrDI	GCAATG	2 cut(s)	506, 1101
BsrI	ACTGG	2 cut(s)	233, 1566
BssECI	CCNNGG	3 cut(s)	29, 1045, 1107
BssMI	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
BssNI	GRCGYC	2 cut(s)	288, 300
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	1 cut(s)	1045
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	3 cut(s)	31, 591, 1109
Bst4CI	ACNGT	7 cut(s)	65, 395, 485, 671, 903, 1577, 1592
Bst6I	CTCTTC	2 cut(s)	100, 152
BstACI	GRCGYC	2 cut(s)	288, 300
BstAPI	GCANNNNNTGC	2 cut(s)	1037, 1412
BstBAI	YACGTR	1 cut(s)	773
BstC8I	GCNNGC	5 cut(s)	18, 36, 1005, 1101, 1613
BstDEI	CTNAG	3 cut(s)	885, 1143, 1539
BstDSI	CCRYGG	1 cut(s)	1045
BstF5I	GGATG	3 cut(s)	270, 1103, 1552
BstFNI	CGCG	4 cut(s)	26, 115, 204, 417
BstH2I	RGCGCY	1 cut(s)	265
BstHHI	GCGC	4 cut(s)	28, 117, 264, 1442
BstKTI	GATC	9 cut(s)	24, 189, 223, 415, 478, 691, 825, 1217, 1570
BstMAI	GTCTC	2 cut(s)	97, 1325
BstMBI	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
BstMCI	CGRYCG	2 cut(s)	24, 415
BstMWI	GCNNNNNNNGC	7 cut(s)	1004, 1037, 1100, 1109, 1241, 1280, 1412
BstNI	CCWGG	3 cut(s)	31, 591, 1109
BstNSI	RCATGY	4 cut(s)	20, 576, 863, 1410
BstSCI	CCNGG	4 cut(s)	29, 182, 589, 1107
BstSFI	CTRYAG	2 cut(s)	915, 1259
BstSLI	GKGCMC	1 cut(s)	8
BstUI	CGCG	4 cut(s)	26, 115, 204, 417
BstV1I	GCAGC	3 cut(s)	174, 177, 572
BstV2I	GAAGAC	2 cut(s)	283, 360
BstX2I	RGATCY	1 cut(s)	1567
BstXI	CCANNNNNNTGG	1 cut(s)	1052
BstYI	RGATCY	1 cut(s)	1567
Bsu36I	CCTNAGG	2 cut(s)	1143, 1539
BsuRI	GGCC	5 cut(s)	182, 520, 780, 1167, 1611
BtgI	CCRYGG	1 cut(s)	1045
BtsCI	GGATG	3 cut(s)	270, 1103, 1552
BtsI	GCAGTG	1 cut(s)	1087
BtsIMutI	CAGTG	2 cut(s)	882, 1087
Cac8I	GCNNGC	5 cut(s)	18, 36, 1005, 1101, 1613
CfoI	GCGC	4 cut(s)	28, 117, 264, 1442
Cfr13I	GGNCC	3 cut(s)	518, 779, 1166
CseI	GACGC	1 cut(s)	308
Csp6I	GTAC	4 cut(s)	419, 486, 1148, 1316
CspCI	CAANNNNNGTGG	2 cut(s)	705, 740
CviQI	GTAC	4 cut(s)	419, 486, 1148, 1316
DdeI	CTNAG	3 cut(s)	885, 1143, 1539
DpnI	GATC	9 cut(s)	23, 188, 222, 414, 477, 690, 824, 1216, 1569
DpnII	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
DraI	TTTAAA	1 cut(s)	541
DraIII	CACNNNGTG	2 cut(s)	888, 1037
DriI	GACNNNNNGTC	1 cut(s)	617
EaeI	YGGCCR	1 cut(s)	180
Eam1104I	CTCTTC	2 cut(s)	100, 152
Eam1105I	GACNNNNNGTC	1 cut(s)	617
EarI	CTCTTC	2 cut(s)	100, 152
Eco130I	CCWWGG	1 cut(s)	1045
Eco24I	GRGCYC	1 cut(s)	585
Eco32I	GATATC	1 cut(s)	371
Eco47III	AGCGCT	1 cut(s)	263
Eco72I	CACGTG	1 cut(s)	773
Eco81I	CCTNAGG	2 cut(s)	1143, 1539
EcoO109I	RGGNCCY	1 cut(s)	779
EcoRI	GAATTC	1 cut(s)	1431
EcoRII	CCWGG	3 cut(s)	29, 589, 1107
EcoRV	GATATC	1 cut(s)	371
EcoT14I	CCWWGG	1 cut(s)	1045
EcoT22I	ATGCAT	3 cut(s)	473, 1285, 1423
EcoT38I	GRGCYC	1 cut(s)	585
ErhI	CCWWGG	1 cut(s)	1045
Esp3I	CGTCTC	1 cut(s)	97
FauNDI	CATATG	2 cut(s)	1130, 1505
Fnu4HI	GCNGC	7 cut(s)	39, 55, 163, 166, 363, 561, 892
FokI	GGATG	3 cut(s)	257, 1090, 1539
FriOI	GRGCYC	1 cut(s)	585
Fsp4HI	GCNGC	7 cut(s)	39, 55, 163, 166, 363, 561, 892
FspBI	CTAG	2 cut(s)	1113, 1448
GlaI	GCGC	4 cut(s)	27, 116, 263, 1441
GluI	GCNGC	7 cut(s)	39, 55, 163, 166, 363, 561, 892
GsaI	CCCAGC	3 cut(s)	519, 786, 1003
GsuI	CTGGAG	1 cut(s)	885
HaeII	RGCGCY	1 cut(s)	265
HaeIII	GGCC	5 cut(s)	182, 520, 780, 1167, 1611
HapII	CCGG	3 cut(s)	183, 344, 366
HgaI	GACGC	1 cut(s)	308
HhaI	GCGC	4 cut(s)	28, 117, 264, 1442
Hin1I	GRCGYC	2 cut(s)	288, 300
Hin6I	GCGC	4 cut(s)	26, 115, 262, 1440
HinP1I	GCGC	4 cut(s)	26, 115, 262, 1440
HindIII	AAGCTT	1 cut(s)	754
HinfI	GANTC	4 cut(s)	174, 1086, 1399, 1534
HpaII	CCGG	3 cut(s)	183, 344, 366
HphI	GGTGA	2 cut(s)	274, 438
Hpy166II	GTNNAC	2 cut(s)	802, 1118
Hpy188I	TCNGA	6 cut(s)	379, 611, 688, 1191, 1426, 1533
Hpy8I	GTNNAC	2 cut(s)	802, 1118
Hpy99I	CGWCG	4 cut(s)	151, 290, 302, 383
HpyAV	CCTTC	2 cut(s)	331, 715
HpyCH4III	ACNGT	7 cut(s)	65, 395, 485, 671, 903, 1577, 1592
HpyCH4IV	ACGT	3 cut(s)	288, 452, 772
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1004, 1037, 1100, 1109, 1241, 1280, 1412
HpyF3I	CTNAG	3 cut(s)	885, 1143, 1539
HpySE526I	ACGT	3 cut(s)	288, 452, 772
Hsp92I	GRCGYC	2 cut(s)	288, 300
HspAI	GCGC	4 cut(s)	26, 115, 262, 1440
KpnI	GGTACC	1 cut(s)	1151
Kzo9I	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
LguI	GCTCTTC	1 cut(s)	152
LmnI	GCTCC	4 cut(s)	496, 866, 1241, 1351
Lsp1109I	GCAGC	3 cut(s)	174, 177, 572
LweI	GCATC	6 cut(s)	862, 1112, 1270, 1292, 1430, 1500
MaeI	CTAG	2 cut(s)	1113, 1448
MaeII	ACGT	3 cut(s)	288, 452, 772
MaeIII	GTNAC	4 cut(s)	59, 395, 444, 568
MalI	GATC	9 cut(s)	23, 188, 222, 414, 477, 690, 824, 1216, 1569
MboI	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
MfeI	CAATTG	2 cut(s)	564, 1497
MflI	RGATCY	1 cut(s)	1567
MhlI	GDGCHC	2 cut(s)	8, 585
MluCI	AATT	9 cut(s)	564, 695, 805, 1012, 1064, 1122, 1252, 1431, 1497
MlyI	GAGTC	1 cut(s)	183
MmeI	TCCRAC	1 cut(s)	402
Mph1103I	ATGCAT	3 cut(s)	473, 1285, 1423
MseI	TTAA	5 cut(s)	540, 597, 905, 956, 1347
MslI	CAYNNNNRTG	3 cut(s)	66, 1094, 1394
MspA1I	CMGCKG	1 cut(s)	1220
MspI	CCGG	3 cut(s)	183, 344, 366
MspR9I	CCNGG	4 cut(s)	31, 184, 591, 1109
MunI	CAATTG	2 cut(s)	564, 1497
Mva1269I	GAATGC	2 cut(s)	473, 1462
MvaI	CCWGG	3 cut(s)	31, 591, 1109
MvnI	CGCG	4 cut(s)	26, 115, 204, 417
MwoI	GCNNNNNNNGC	7 cut(s)	1004, 1037, 1100, 1109, 1241, 1280, 1412
NciI	CCSGG	1 cut(s)	184
NcoI	CCATGG	1 cut(s)	1045
NdeI	CATATG	2 cut(s)	1130, 1505
NdeII	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
NlaIV	GGNNCC	6 cut(s)	463, 781, 868, 1149, 1569, 1589
NmuCI	GTSAC	2 cut(s)	59, 444
NsiI	ATGCAT	3 cut(s)	473, 1285, 1423
NspI	RCATGY	4 cut(s)	20, 576, 863, 1410
PaeI	GCATGC	1 cut(s)	20
PciSI	GCTCTTC	1 cut(s)	152
PcsI	WCGNNNNNNNCGW	6 cut(s)	143, 201, 216, 294, 449, 1408
PctI	GAATGC	2 cut(s)	473, 1462
PfeI	GAWTC	3 cut(s)	1086, 1399, 1534
PkrI	GCNGC	7 cut(s)	40, 56, 164, 167, 364, 562, 893
Ple19I	CGATCG	2 cut(s)	24, 415
PleI	GAGTC	1 cut(s)	182
PmaCI	CACGTG	1 cut(s)	773
PmlI	CACGTG	1 cut(s)	773
PpsI	GAGTC	1 cut(s)	182
Ppu21I	YACGTR	1 cut(s)	773
Psp6I	CCWGG	3 cut(s)	29, 589, 1107
PspCI	CACGTG	1 cut(s)	773
PspFI	CCCAGC	3 cut(s)	515, 782, 999
PspGI	CCWGG	3 cut(s)	29, 589, 1107
PspN4I	GGNNCC	6 cut(s)	463, 781, 868, 1149, 1569, 1589
PspPI	GGNCC	3 cut(s)	518, 779, 1166
PsuI	RGATCY	1 cut(s)	1567
PvuI	CGATCG	2 cut(s)	24, 415
PvuII	CAGCTG	1 cut(s)	1220
RgaI	GCGATCGC	1 cut(s)	24
RsaI	GTAC	4 cut(s)	420, 487, 1149, 1317
RsaNI	GTAC	4 cut(s)	419, 486, 1148, 1316
RseI	CAYNNNNRTG	3 cut(s)	66, 1094, 1394
SapI	GCTCTTC	1 cut(s)	152
SaqAI	TTAA	5 cut(s)	540, 597, 905, 956, 1347
SatI	GCNGC	7 cut(s)	39, 55, 163, 166, 363, 561, 892
Sau3AI	GATC	9 cut(s)	21, 186, 220, 412, 475, 688, 822, 1214, 1567
Sau96I	GGNCC	3 cut(s)	518, 779, 1166
SchI	GAGTC	1 cut(s)	183
ScrFI	CCNGG	4 cut(s)	31, 184, 591, 1109
SduI	GDGCHC	2 cut(s)	8, 585
SfaAI	GCGATCGC	1 cut(s)	24
SfaNI	GCATC	6 cut(s)	862, 1112, 1270, 1292, 1430, 1500
SfcI	CTRYAG	2 cut(s)	915, 1259
SgfI	GCGATCGC	1 cut(s)	24
SmiMI	CAYNNNNRTG	3 cut(s)	66, 1094, 1394
SmlI	CTYRAG	3 cut(s)	329, 425, 715
SmoI	CTYRAG	3 cut(s)	329, 425, 715
SphI	GCATGC	1 cut(s)	20
Sse9I	AATT	9 cut(s)	564, 695, 805, 1012, 1064, 1122, 1252, 1431, 1497
SsiI	CCGC	7 cut(s)	39, 52, 55, 202, 363, 576, 892
SspMI	CTAG	2 cut(s)	1113, 1448
StyD4I	CCNGG	4 cut(s)	29, 182, 589, 1107
StyI	CCWWGG	1 cut(s)	1045
TaaI	ACNGT	7 cut(s)	65, 395, 485, 671, 903, 1577, 1592
TaiI	ACGT	3 cut(s)	291, 455, 775
TaqI	TCGA	7 cut(s)	106, 172, 210, 255, 351, 978, 1579
TasI	AATT	9 cut(s)	564, 695, 805, 1012, 1064, 1122, 1252, 1431, 1497
TatI	WGTACW	2 cut(s)	485, 1315
TauI	GCSGC	4 cut(s)	41, 57, 365, 894
TfiI	GAWTC	3 cut(s)	1086, 1399, 1534
Tru1I	TTAA	5 cut(s)	540, 597, 905, 956, 1347
Tru9I	TTAA	5 cut(s)	540, 597, 905, 956, 1347
TscAI	CASTG	2 cut(s)	889, 1087
TseFI	GTSAC	2 cut(s)	59, 444
TseI	GCWGC	3 cut(s)	162, 165, 560
Tsp45I	GTSAC	2 cut(s)	59, 444
TspDTI	ATGAA	5 cut(s)	497, 728, 1078, 1454, 1612
TspGWI	ACGGA	1 cut(s)	79
TspRI	CASTG	2 cut(s)	889, 1087
XapI	RAATTY	3 cut(s)	1012, 1252, 1431
XceI	RCATGY	4 cut(s)	20, 576, 863, 1410
XspI	CTAG	2 cut(s)	1113, 1448
ZraI	GACGTC	1 cut(s)	289
Zsp2I	ATGCAT	3 cut(s)	473, 1285, 1423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.