MD05G1311600.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004034
Physical Location & Seq
Reverse (-)
44161243 .. 44161779
537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1311600.v1.1.491

Sequence Viewer

Length: 537 bp
ATGGCGACTGCAGCTGCTCCCTCTTCCTCCCGCGAGGGCCACTACAGGGGCGTGAGGAAGCGTCCATGGGGCCGGTACGCCGCAGAAATCCGGGACCCCTGGAAGAAGACGAGGGTCTGGCTCGGCACATTCGACACGCCGGAGGAAGCCGCCCTCGCCTACGACGGCGCCGCCAGGACGCTCCGCGGCGCCAAGGCCAAGACCAACTTCCCTGCGCCGATCCCCAGCGGCATCTCCCTCGATCTCAACGCTCCCTCCGCCGCCCACTGGGCCACTCACTCCGGCCGCCTCGTCGAATTCCTCCACACCGGCGTCCTCAACGACATGGGCGGCGGCACTTCCACGAATGATACGCTGCCGTTGATGGCGAGGAGGGAACCTCAGCAAGCTCCCGTTGGTGTGGCGGGAAATGTCGGCGGCGGTGGTAATGGCACGGTCGCGGATGGTTCGGGTTTCGGCAGTTCGGCGTCTTTTCTGGGACTGGTCCGGCGTGGCTTGCCGATTGATCTCAATGAGCCTCCTCCCTTGTGGCTGTGA

Protein Analysis

179

Amino Acids

18.7

Weight (kDa)

9.76

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 14 - 63 1.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 167, 188
AccII CGCG 3 cut(s) 33, 186, 440
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 296
AcyI GRCGYC 4 cut(s) 168, 189, 312, 467
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 2 cut(s) 46, 267
AjnI CCWGG 2 cut(s) 98, 173
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 2 cut(s) 14, 389
AluI AGCT 2 cut(s) 14, 389
AlwI GGATC 1 cut(s) 214
AoxI GGCC 5 cut(s) 37, 70, 195, 270, 283
ApeKI GCWGC 3 cut(s) 11, 14, 355
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 3 cut(s) 170, 191, 217
AspS9I GGNCC 5 cut(s) 37, 70, 94, 270, 484
AsuC2I CCSGG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 94, 484
BanI GGYRCC 2 cut(s) 167, 188
BbsI GAAGAC 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 2 cut(s) 23, 342
BccI CCATC 2 cut(s) 358, 437
BceAI ACGGC 2 cut(s) 181, 343
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BciT130I CCWGG 2 cut(s) 100, 175
BcnI CCSGG 1 cut(s) 92
BfmI CTRYAG 2 cut(s) 9, 43
BfoI RGCGCY 2 cut(s) 171, 192
BglI GCCNNNNNGGC 1 cut(s) 269
Bme1390I CCNGG 3 cut(s) 92, 100, 175
Bme18I GGWCC 2 cut(s) 94, 484
BmgT120I GGNCC 5 cut(s) 37, 70, 94, 270, 484
BmiI GGNNCC 6 cut(s) 71, 95, 96, 169, 190, 378
BmrFI CCNGG 3 cut(s) 92, 100, 175
BmrI ACTGGG 1 cut(s) 277
BmsI GCATC 1 cut(s) 240
BmuI ACTGGG 1 cut(s) 277
BoxI GACNNNNGTC 1 cut(s) 113
BpiI GAAGAC 1 cut(s) 113
BplI GAGNNNNNCTC 2 cut(s) 364, 396
Bpu10I CCTNAGC 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 4 cut(s) 168, 189, 312, 467
BsaJI CCNNGG 4 cut(s) 65, 98, 184, 192
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 267
Bse118I RCCGGY 2 cut(s) 72, 308
Bse1I ACTGG 2 cut(s) 272, 486
BseBI CCWGG 2 cut(s) 100, 175
BseDI CCNNGG 4 cut(s) 65, 98, 184, 192
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 2 cut(s) 46, 267
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 2 cut(s) 272, 486
BseRI GAGGAG 2 cut(s) 385, 510
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 23, 342
BseYI CCCAGC 1 cut(s) 224
Bsh1236I CGCG 3 cut(s) 33, 186, 440
Bsh1285I CGRYCG 2 cut(s) 286, 438
BshFI GGCC 5 cut(s) 39, 72, 197, 272, 285
BshNI GGYRCC 2 cut(s) 167, 188
BsiEI CGRYCG 2 cut(s) 286, 438
BsiSI CCGG 6 cut(s) 73, 91, 140, 282, 309, 487
BslFI GGGAC 2 cut(s) 107, 492
BslI CCNNNNNNNGG 2 cut(s) 46, 267
BsmFI GGGAC 2 cut(s) 107, 492
BsnI GGCC 5 cut(s) 39, 72, 197, 272, 285
Bsp143I GATC 3 cut(s) 219, 241, 505
Bsp19I CCATGG 1 cut(s) 65
BspANI GGCC 5 cut(s) 39, 72, 197, 272, 285
BspCNI CTCAG 1 cut(s) 394
BspFNI CGCG 3 cut(s) 33, 186, 440
BspLI GGNNCC 6 cut(s) 71, 95, 96, 169, 190, 378
BspMAI CTGCAG 1 cut(s) 13
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 2 cut(s) 167, 188
BsrFI RCCGGY 2 cut(s) 72, 308
BsrI ACTGG 2 cut(s) 272, 486
BssAI RCCGGY 2 cut(s) 72, 308
BssECI CCNNGG 4 cut(s) 65, 98, 184, 192
BssMI GATC 3 cut(s) 219, 241, 505
BssNI GRCGYC 4 cut(s) 168, 189, 312, 467
BssT1I CCWWGG 2 cut(s) 65, 192
Bst2UI CCWGG 2 cut(s) 100, 175
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 28
BstACI GRCGYC 4 cut(s) 168, 189, 312, 467
BstC8I GCNNGC 2 cut(s) 387, 497
BstDEI CTNAG 1 cut(s) 381
BstDSI CCRYGG 2 cut(s) 65, 184
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 3 cut(s) 33, 186, 440
BstH2I RGCGCY 2 cut(s) 171, 192
BstHHI GCGC 3 cut(s) 170, 191, 217
BstKTI GATC 3 cut(s) 222, 244, 508
BstMBI GATC 3 cut(s) 219, 241, 505
BstMCI CGRYCG 2 cut(s) 286, 438
BstMWI GCNNNNNNNGC 5 cut(s) 11, 155, 257, 269, 496
BstNI CCWGG 2 cut(s) 100, 175
BstPAI GACNNNNGTC 1 cut(s) 113
BstSCI CCNGG 3 cut(s) 90, 98, 173
BstSFI CTRYAG 2 cut(s) 9, 43
BstUI CGCG 3 cut(s) 33, 186, 440
BstV1I GCAGC 2 cut(s) 23, 342
BstV2I GAAGAC 1 cut(s) 113
BstZI CGGCCG 1 cut(s) 283
BsuRI GGCC 5 cut(s) 39, 72, 197, 272, 285
BtgI CCRYGG 2 cut(s) 65, 184
BtsCI GGATG 1 cut(s) 448
BtsIMutI CAGTG 1 cut(s) 265
Cac8I GCNNGC 2 cut(s) 387, 497
CfoI GCGC 3 cut(s) 170, 191, 217
Cfr10I RCCGGY 2 cut(s) 72, 308
Cfr13I GGNCC 5 cut(s) 37, 70, 94, 270, 484
Cfr42I CCGCGG 1 cut(s) 187
CseI GACGC 4 cut(s) 50, 187, 301, 456
Csp6I GTAC 1 cut(s) 76
CviAII CATG 2 cut(s) 66, 325
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 1 cut(s) 381
DinI GGCGCC 2 cut(s) 169, 190
DpnI GATC 3 cut(s) 221, 243, 507
DpnII GATC 3 cut(s) 219, 241, 505
EaeI YGGCCR 1 cut(s) 283
EagI CGGCCG 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
EciI GGCGGA 1 cut(s) 247
EclXI CGGCCG 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 65, 192
Eco47I GGWCC 2 cut(s) 94, 484
Eco52I CGGCCG 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 2 cut(s) 98, 173
EcoT14I CCWWGG 2 cut(s) 65, 192
EgeI GGCGCC 2 cut(s) 169, 190
EheI GGCGCC 2 cut(s) 169, 190
ErhI CCWWGG 2 cut(s) 65, 192
FaeI CATG 2 cut(s) 69, 328
FaiI YATR 2 cut(s) 67, 326
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FaqI GGGAC 2 cut(s) 107, 492
FatI CATG 2 cut(s) 65, 324
FauI CCCGC 2 cut(s) 38, 397
FokI GGATG 1 cut(s) 455
GlaI GCGC 3 cut(s) 169, 190, 216
GsaI CCCAGC 1 cut(s) 228
HaeII RGCGCY 2 cut(s) 171, 192
HaeIII GGCC 5 cut(s) 39, 72, 197, 272, 285
HapII CCGG 6 cut(s) 73, 91, 140, 282, 309, 487
HgaI GACGC 4 cut(s) 50, 187, 301, 456
HhaI GCGC 3 cut(s) 170, 191, 217
Hin1I GRCGYC 4 cut(s) 168, 189, 312, 467
Hin1II CATG 2 cut(s) 69, 328
Hin6I GCGC 3 cut(s) 168, 189, 215
HinP1I GCGC 3 cut(s) 168, 189, 215
HpaII CCGG 6 cut(s) 73, 91, 140, 282, 309, 487
Hpy99I CGWCG 2 cut(s) 167, 296
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 1 cut(s) 11
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 155, 257, 269, 496
HpyF3I CTNAG 1 cut(s) 381
Hsp92I GRCGYC 4 cut(s) 168, 189, 312, 467
Hsp92II CATG 2 cut(s) 69, 328
HspAI GCGC 3 cut(s) 168, 189, 215
KasI GGCGCC 2 cut(s) 167, 188
KflI GGGWCCC 1 cut(s) 94
KspI CCGCGG 1 cut(s) 187
Kzo9I GATC 3 cut(s) 219, 241, 505
LmnI GCTCC 4 cut(s) 22, 186, 256, 394
Lsp1109I GCAGC 2 cut(s) 23, 342
LweI GCATC 1 cut(s) 240
MalI GATC 3 cut(s) 221, 243, 507
MboI GATC 3 cut(s) 219, 241, 505
MboII GAAGA 3 cut(s) 15, 115, 118
MluCI AATT 1 cut(s) 296
Mly113I GGCGCC 2 cut(s) 168, 189
MspA1I CMGCKG 3 cut(s) 14, 186, 228
MspI CCGG 6 cut(s) 73, 91, 140, 282, 309, 487
MspR9I CCNGG 3 cut(s) 92, 100, 175
MvaI CCWGG 2 cut(s) 100, 175
MvnI CGCG 3 cut(s) 33, 186, 440
MwoI GCNNNNNNNGC 5 cut(s) 11, 155, 257, 269, 496
NarI GGCGCC 2 cut(s) 168, 189
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 65
NdeII GATC 3 cut(s) 219, 241, 505
NlaIII CATG 2 cut(s) 69, 328
NlaIV GGNNCC 6 cut(s) 71, 95, 96, 169, 190, 378
NmeAIII GCCGAG 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 90
PluTI GGCGCC 2 cut(s) 171, 192
PpuMI RGGWCCY 1 cut(s) 94
PshAI GACNNNNGTC 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 2 cut(s) 98, 173
PspFI CCCAGC 1 cut(s) 224
PspGI CCWGG 2 cut(s) 98, 173
PspN4I GGNNCC 6 cut(s) 71, 95, 96, 169, 190, 378
PspPI GGNCC 5 cut(s) 37, 70, 94, 270, 484
PspPPI RGGWCCY 1 cut(s) 94
PstI CTGCAG 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SacII CCGCGG 1 cut(s) 187
Sau3AI GATC 3 cut(s) 219, 241, 505
Sau96I GGNCC 5 cut(s) 37, 70, 94, 270, 484
ScrFI CCNGG 3 cut(s) 92, 100, 175
SetI ASST 3 cut(s) 16, 382, 391
SfaNI GCATC 1 cut(s) 240
SfcI CTRYAG 2 cut(s) 9, 43
SfoI GGCGCC 2 cut(s) 169, 190
Sfr303I CCGCGG 1 cut(s) 187
SgrAI CRCCGGYG 1 cut(s) 308
SgrBI CCGCGG 1 cut(s) 187
SinI GGWCC 2 cut(s) 94, 484
Sse9I AATT 1 cut(s) 296
SspDI GGCGCC 2 cut(s) 167, 188
StyD4I CCNGG 3 cut(s) 90, 98, 173
StyI CCWWGG 2 cut(s) 65, 192
TaaI ACNGT 1 cut(s) 436
TaqI TCGA 3 cut(s) 132, 240, 294
TasI AATT 1 cut(s) 296
TscAI CASTG 1 cut(s) 272
TseI GCWGC 3 cut(s) 11, 14, 355
TspRI CASTG 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 94, 484
XapI RAATTY 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.