AT1G30590

RNA polymerase I-specific transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004110
Physical Location & Seq
Reverse (-)
10835735 .. 10840872
5138 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30590.2

Sequence Viewer

Length: 1815 bp
ATGGGAGCAGAAGAATTTCCGAGTGTTCCGTTCAACTCAAATGCAATGGACAACGCTGAATATACCGACACAGATTTGGTGTTTGCCGTTAGAAAGGCCCTTGCATCTGTCCAAAATGGAGATACCGACGATTATAGTCAGCTTAAAACAGTGATGTGCCTTACAGAAGACGCTGATTTCGATGCAGTGGCACAGCTTGAGACAGTCTTGAAAAGTCTATCAGTTTCCGTTGCTTGGATAGATTTAGTTCATCATAAAGATCTTCTTGAAGCTATGAGCTTGTGGTATCATAGTCACAGACCTAGTGTAATGGATGCATTGGTGGACCTAATCATATCACTGGCCGCTACTAGTGGAAAATATCTGGATCCTTGTTTGAATATGCTCGTAAGAAATTTCAGTCAACCTACTTTTAAGCATAAAGTTTCACAAACCCAATTAGTCAAAAAGATGCAGGAAGTTCATCCACGGGTGCATGCAGCCCTTCACAAGATTTCTTATTTAATTCCTCTTGCTCCCTGGAATTTAGTGTCTATACTTGCGCAGAACATGCGTAAAATTGACAAAAAGGACCCTTCTATAGTGACATATGTGGATAACCTGTTGAGGTTGGAGAATAGCTCAATCGGAGAAGTTGTTGGCAGCGTGATTCTTATGATGGTAATGGAGAGGATGCTAGATTTAGATTTGGTAAGTGGATGTGATGACTCTAATGGAGGCATGTTTGATATGGAACTTGAAGATGCAGTTGAAAGCACTATGAATGAAGGAGACGAGTTTCCAGTGGGGGCTCTAAAACAAAATACTTCAGGTGGAAATGTAGTCTCTGAACTGTTGGACAAATTGATGGTCCTATTTTTTCATCATCTAGAATCCTGTCAAAACTCTGATCGTTTGGATGAGGTATTTGAAATCCTCTTTAAGTCGTTCGAGAACTATATTCTGAACACATACAAAACAAAATTTTCACAGTTTCTGATGTTCTATGCATGCTCACTAGATCCTGAAAATTGTGGTGTGAGATTTGCCAGTAAGCTGTTGGACATATATCTCTCCAGCAACACATGTCGACTTACTAGGATGAGTGCAGTGGCTTATCTAGCTAGCTATTTGTCTCGTGGAAAGTTTTTGCCTGCTTCTTTTGTGGCTAGCATGTTGAAAAGATTGGTGGACGAGTGTGCGGAATATTGCGGAACATGCAATGATGATGTGAAGCCAGAAGCACATCAAGTGTTCTATTCTGGATGTCAGGCAATCTTGTATGTGCTATGCTTCCGAATGAGATCCATAGTGGAGATTCCTCGCTTTCAATCGCAGTTTAGATCATTGGAGTCAATTTTATCTCACAAACTAAACCCACTACTGGTGTGTCTTCCATCTGTAGTTTCCGAGTTCCTTAAACAAGCCAAAGCTGGTGGTCTGTTCATTGTCTCAGAATCCTTCATTTTCGATGACCTACACGAGTCTGAGCTCTCTCGTGCTTTTGGTGGCTTTGAAAGGCTTGACACATTCTTCCCGTTTGACCCGTGCTTGTTGAAAATGTCTAGCAGCTACATCTCCCCGAACTTCAACTTCTGGTCAATGGTGAAAACGACTTATGGAGAAGATGGTGACGAAGAGCTTTGTGATGAGGTCATAGTGAATGGAGATGCAGACAGTGCGGAGGAACCTGACGATGACGTTGAACTTGATAGTGAGATGAACACGATGTCTACAACTCCGAAACACTCTTTCATGCGAGAAACAGAGAGGCTCTTGAAGATGCCTTCAAGAATCAGACCATCCACTAGTCCTCCTGAATCCTTCTTAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRN3

Protein Analysis

604

Amino Acids

68.06

Weight (kDa)

4.74

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRN3 PF05327 21 - 533 7.8e-119 RNA polymerase I specific transcription initiation factor RRN3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 543
AccI GTMKAC 2 cut(s) 1069, 1712
AciI CCGC 4 cut(s) 345, 1181, 1191, 1661
AclWI GGATC 4 cut(s) 362, 375, 995, 1278
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 4 cut(s) 14, 394, 523, 962
AcuI CTGAAG 1 cut(s) 792
AfiI CCNNNNNNNGG 2 cut(s) 234, 1363
AflIII ACRYGT 1 cut(s) 1064
AhlI ACTAGT 2 cut(s) 350, 1787
AjnI CCWGG 1 cut(s) 518
Alw21I GWGCWC 1 cut(s) 1473
Alw26I GTCTC 5 cut(s) 194, 765, 829, 1119, 1435
AlwI GGATC 4 cut(s) 362, 375, 995, 1278
AlwNI CAGNNNCTG 1 cut(s) 976
AoxI GGCC 2 cut(s) 96, 342
ApeKI GCWGC 3 cut(s) 479, 642, 1548
ApoI RAATTY 4 cut(s) 14, 394, 523, 962
ArsI GACNNNNNNTTYG 4 cut(s) 161, 193, 834, 866
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 4 cut(s) 97, 325, 571, 850
AsuHPI GGTGA 2 cut(s) 1597, 1622
AsuNHI GCTAGC 2 cut(s) 1103, 1148
AvaII GGWCC 3 cut(s) 325, 571, 850
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 2 cut(s) 793, 1473
BauI CACGAG 3 cut(s) 1116, 1460, 1476
BbsI GAAGAC 2 cut(s) 174, 1364
Bbv12I GWGCWC 1 cut(s) 1473
BbvI GCAGC 3 cut(s) 491, 654, 1560
BccI CCATC 5 cut(s) 652, 841, 1384, 1601, 1789
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 1 cut(s) 520
BcoDI GTCTC 5 cut(s) 194, 765, 829, 1119, 1435
BcuI ACTAGT 2 cut(s) 350, 1787
BfmI CTRYAG 2 cut(s) 579, 1380
BglII AGATCT 1 cut(s) 259
BisI GCNGC 4 cut(s) 345, 480, 643, 1549
BlsI GCNGC 4 cut(s) 346, 481, 644, 1550
Bme1390I CCNGG 1 cut(s) 520
Bme18I GGWCC 3 cut(s) 325, 571, 850
BmgT120I GGNCC 4 cut(s) 97, 325, 571, 850
BmiI GGNNCC 3 cut(s) 369, 573, 1668
BmrFI CCNGG 1 cut(s) 520
BmsI GCATC 8 cut(s) 113, 172, 304, 441, 663, 733, 1639, 1752
BmtI GCTAGC 2 cut(s) 1107, 1152
BpiI GAAGAC 2 cut(s) 174, 1364
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 1 cut(s) 1039
BpuEI CTTGAG 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 467, 518
BsaXI ACNNNNNCTCC 4 cut(s) 621, 651, 1777, 1807
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 1363
Bse1I ACTGG 4 cut(s) 345, 782, 1029, 1368
Bse3DI GCAATG 2 cut(s) 51, 1207
BseBI CCWGG 1 cut(s) 520
BseDI CCNNGG 2 cut(s) 467, 518
BseGI GGATG 8 cut(s) 319, 463, 678, 704, 904, 1086, 1250, 1781
BseLI CCNNNNNNNGG 2 cut(s) 234, 1363
BseMI GCAATG 2 cut(s) 51, 1207
BseMII CTCAG 2 cut(s) 1446, 1458
BseNI ACTGG 4 cut(s) 345, 782, 1029, 1368
BseXI GCAGC 3 cut(s) 491, 654, 1560
BsgI GTGCAG 1 cut(s) 1107
BshFI GGCC 2 cut(s) 98, 344
BsiHKAI GWGCWC 1 cut(s) 1473
BslI CCNNNNNNNGG 2 cut(s) 234, 1363
BsmAI GTCTC 5 cut(s) 194, 765, 829, 1119, 1435
BsmBI CGTCTC 1 cut(s) 765
BsnI GGCC 2 cut(s) 98, 344
Bsp1286I GDGCHC 2 cut(s) 793, 1473
Bsp143I GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
BspACI CCGC 4 cut(s) 345, 1181, 1191, 1661
BspANI GGCC 2 cut(s) 98, 344
BspCNI CTCAG 2 cut(s) 1445, 1459
BspLI GGNNCC 3 cut(s) 369, 573, 1668
BspOI GCTAGC 2 cut(s) 1107, 1152
BspPI GGATC 4 cut(s) 362, 375, 995, 1278
BspQI GCTCTTC 1 cut(s) 1611
BsrDI GCAATG 2 cut(s) 51, 1207
BsrI ACTGG 4 cut(s) 345, 782, 1029, 1368
BssECI CCNNGG 2 cut(s) 467, 518
BssMI GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
BssSI CACGAG 3 cut(s) 1116, 1460, 1476
Bst2BI CACGAG 3 cut(s) 1116, 1460, 1476
Bst2UI CCWGG 1 cut(s) 520
Bst4CI ACNGT 5 cut(s) 151, 205, 834, 972, 1658
Bst6I CTCTTC 1 cut(s) 1611
BstAPI GCANNNNNTGC 2 cut(s) 550, 1658
BstC8I GCNNGC 5 cut(s) 477, 991, 1105, 1134, 1150
BstDEI CTNAG 2 cut(s) 1432, 1467
BstDSI CCRYGG 1 cut(s) 467
BstF5I GGATG 8 cut(s) 319, 463, 678, 704, 904, 1086, 1250, 1781
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 6 cut(s) 262, 370, 892, 1003, 1286, 1325
BstMAI GTCTC 5 cut(s) 194, 765, 829, 1119, 1435
BstMBI GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
BstMWI GCNNNNNNNGC 4 cut(s) 550, 1100, 1197, 1658
BstNI CCWGG 1 cut(s) 520
BstNSI RCATGY 7 cut(s) 479, 553, 724, 993, 1068, 1156, 1200
BstSCI CCNGG 1 cut(s) 518
BstSFI CTRYAG 2 cut(s) 579, 1380
BstV1I GCAGC 3 cut(s) 491, 654, 1560
BstV2I GAAGAC 2 cut(s) 174, 1364
BstX2I RGATCY 4 cut(s) 259, 367, 1000, 1283
BstYI RGATCY 4 cut(s) 259, 367, 1000, 1283
BsuRI GGCC 2 cut(s) 98, 344
BtgI CCRYGG 1 cut(s) 467
BtsCI GGATG 8 cut(s) 319, 463, 678, 704, 904, 1086, 1250, 1781
BtsI GCAGTG 2 cut(s) 192, 1095
BtsIMutI CAGTG 6 cut(s) 156, 192, 338, 789, 1095, 1663
Cac8I GCNNGC 5 cut(s) 477, 991, 1105, 1134, 1150
CaiI CAGNNNCTG 1 cut(s) 976
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 4 cut(s) 97, 325, 571, 850
CseI GACGC 1 cut(s) 179
CspCI CAANNNNNGTGG 2 cut(s) 1396, 1431
CviAII CATG 8 cut(s) 476, 550, 721, 990, 1065, 1153, 1197, 1735
DdeI CTNAG 2 cut(s) 1432, 1467
DpnI GATC 6 cut(s) 261, 369, 891, 1002, 1285, 1324
DpnII GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 1611
EarI CTCTTC 1 cut(s) 1611
Ecl136II GAGCTC 1 cut(s) 1471
Eco24I GRGCYC 2 cut(s) 793, 1473
Eco47I GGWCC 3 cut(s) 325, 571, 850
Eco53kI GAGCTC 1 cut(s) 1471
Eco57I CTGAAG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 1471
EcoO109I RGGNCCY 2 cut(s) 97, 571
EcoRII CCWGG 1 cut(s) 518
EcoT22I ATGCAT 2 cut(s) 319, 991
EcoT38I GRGCYC 2 cut(s) 793, 1473
Esp3I CGTCTC 1 cut(s) 765
FaeI CATG 8 cut(s) 479, 553, 724, 993, 1068, 1156, 1200, 1738
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 8 cut(s) 475, 549, 720, 989, 1064, 1152, 1196, 1734
FauNDI CATATG 1 cut(s) 589
FblI GTMKAC 2 cut(s) 1069, 1712
Fnu4HI GCNGC 4 cut(s) 345, 480, 643, 1549
FokI GGATG 8 cut(s) 326, 450, 685, 711, 911, 1093, 1257, 1768
FriOI GRGCYC 2 cut(s) 793, 1473
Fsp4HI GCNGC 4 cut(s) 345, 480, 643, 1549
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 1 cut(s) 543
GluI GCNGC 4 cut(s) 345, 480, 643, 1549
GsuI CTGGAG 1 cut(s) 1039
HaeIII GGCC 2 cut(s) 98, 344
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 544
Hin1II CATG 8 cut(s) 479, 553, 724, 993, 1068, 1156, 1200, 1738
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 2 cut(s) 404, 1070
HindII GTYRAC 2 cut(s) 404, 1070
HinfI GANTC 9 cut(s) 649, 707, 872, 1297, 1331, 1436, 1463, 1773, 1799
HphI GGTGA 2 cut(s) 1597, 1622
Hpy166II GTNNAC 5 cut(s) 325, 404, 1070, 1171, 1713
Hpy8I GTNNAC 5 cut(s) 325, 404, 1070, 1171, 1713
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 6 cut(s) 494, 585, 761, 1450, 1776, 1813
HpyCH4III ACNGT 5 cut(s) 151, 205, 834, 972, 1658
HpyCH4IV ACGT 1 cut(s) 1680
HpyF10VI GCNNNNNNNGC 4 cut(s) 550, 1100, 1197, 1658
HpyF3I CTNAG 2 cut(s) 1432, 1467
HpySE526I ACGT 1 cut(s) 1680
Hsp92II CATG 8 cut(s) 479, 553, 724, 993, 1068, 1156, 1200, 1738
HspAI GCGC 1 cut(s) 542
Kzo9I GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
LguI GCTCTTC 1 cut(s) 1611
LmnI GCTCC 2 cut(s) 5, 520
Lsp1109I GCAGC 3 cut(s) 491, 654, 1560
LweI GCATC 8 cut(s) 113, 172, 304, 441, 663, 733, 1639, 1752
MaeII ACGT 1 cut(s) 1680
MaeIII GTNAC 3 cut(s) 293, 583, 1610
MalI GATC 6 cut(s) 261, 369, 891, 1002, 1285, 1324
MboI GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
MboII GAAGA 9 cut(s) 23, 179, 254, 752, 1364, 1504, 1616, 1628, 1771
MflI RGATCY 4 cut(s) 259, 367, 1000, 1283
MhlI GDGCHC 2 cut(s) 793, 1473
MlyI GAGTC 3 cut(s) 701, 1340, 1472
MmeI TCCRAC 3 cut(s) 591, 816, 1020
Mph1103I ATGCAT 2 cut(s) 319, 991
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 6 cut(s) 144, 414, 503, 921, 1398, 1808
MspR9I CCNGG 1 cut(s) 520
MvaI CCWGG 1 cut(s) 520
MwoI GCNNNNNNNGC 4 cut(s) 550, 1100, 1197, 1658
NdeI CATATG 1 cut(s) 589
NdeII GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
NheI GCTAGC 2 cut(s) 1103, 1148
NlaIII CATG 8 cut(s) 479, 553, 724, 993, 1068, 1156, 1200, 1738
NlaIV GGNNCC 3 cut(s) 369, 573, 1668
NmuCI GTSAC 3 cut(s) 293, 583, 1610
NsbI TGCGCA 1 cut(s) 543
NsiI ATGCAT 2 cut(s) 319, 991
NspI RCATGY 7 cut(s) 479, 553, 724, 993, 1068, 1156, 1200
PaeI GCATGC 2 cut(s) 479, 993
PciI ACATGT 1 cut(s) 1064
PciSI GCTCTTC 1 cut(s) 1611
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 6 cut(s) 649, 872, 1297, 1436, 1773, 1799
PkrI GCNGC 4 cut(s) 346, 481, 644, 1550
PleI GAGTC 3 cut(s) 701, 1339, 1471
PpsI GAGTC 3 cut(s) 701, 1339, 1471
PpuMI RGGWCCY 1 cut(s) 571
PscI ACATGT 1 cut(s) 1064
Psp124BI GAGCTC 1 cut(s) 1473
Psp5II RGGWCCY 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 518
PspGI CCWGG 1 cut(s) 518
PspN4I GGNNCC 3 cut(s) 369, 573, 1668
PspPI GGNCC 4 cut(s) 97, 325, 571, 850
PspPPI RGGWCCY 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 976
PsuI RGATCY 4 cut(s) 259, 367, 1000, 1283
SacI GAGCTC 1 cut(s) 1473
SalI GTCGAC 1 cut(s) 1068
SapI GCTCTTC 1 cut(s) 1611
SaqAI TTAA 6 cut(s) 144, 414, 503, 921, 1398, 1808
SatI GCNGC 4 cut(s) 345, 480, 643, 1549
Sau3AI GATC 6 cut(s) 259, 367, 889, 1000, 1283, 1322
Sau96I GGNCC 4 cut(s) 97, 325, 571, 850
SchI GAGTC 3 cut(s) 701, 1340, 1472
ScrFI CCNGG 1 cut(s) 520
SduI GDGCHC 2 cut(s) 793, 1473
SfaNI GCATC 8 cut(s) 113, 172, 304, 441, 663, 733, 1639, 1752
SfcI CTRYAG 2 cut(s) 579, 1380
SinI GGWCC 3 cut(s) 325, 571, 850
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
SpeI ACTAGT 2 cut(s) 350, 1787
SphI GCATGC 2 cut(s) 479, 993
SsiI CCGC 4 cut(s) 345, 1181, 1191, 1661
SspI AATATT 1 cut(s) 1187
SstI GAGCTC 1 cut(s) 1473
StyD4I CCNGG 1 cut(s) 518
TaaI ACNGT 5 cut(s) 151, 205, 834, 972, 1658
TaiI ACGT 1 cut(s) 1683
TaqI TCGA 4 cut(s) 180, 930, 1069, 1449
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 6 cut(s) 649, 872, 1297, 1436, 1773, 1799
Tru1I TTAA 6 cut(s) 144, 414, 503, 921, 1398, 1808
Tru9I TTAA 6 cut(s) 144, 414, 503, 921, 1398, 1808
TscAI CASTG 6 cut(s) 156, 192, 345, 789, 1095, 1663
TseFI GTSAC 3 cut(s) 293, 583, 1610
TseI GCWGC 3 cut(s) 479, 642, 1548
Tsp45I GTSAC 3 cut(s) 293, 583, 1610
TspDTI ATGAA 9 cut(s) 239, 452, 776, 780, 851, 1414, 1432, 1715, 1723
TspGWI ACGGA 2 cut(s) 18, 217
TspRI CASTG 6 cut(s) 156, 192, 345, 789, 1095, 1663
VpaK11BI GGWCC 3 cut(s) 325, 571, 850
XapI RAATTY 4 cut(s) 14, 394, 523, 962
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 7 cut(s) 479, 553, 724, 993, 1068, 1156, 1200
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XmiI GTMKAC 2 cut(s) 1069, 1712
XmnI GAANNNNTTC 1 cut(s) 15
Zsp2I ATGCAT 2 cut(s) 319, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.