Rroxscaffold_2G00145300

RNA polymerase I-specific transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004110
Physical Location & Seq
Forward (+)
83218747 .. 83223972
5226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145300.1

Sequence Viewer

Length: 1845 bp
ATGGGCGTCGAGCTCCGGCAAGACCTTGATATGGACGACGGCGGCGTTTACTCCGACTACGACCTAGCTCTACACGTCAAGGATGTTCTCCTCTCAGTCTCCTCTGGCGATGTTGTGTCTGTTGAGAACTACAGAGAGCTCGTCGGGCGTCTGTACCCTAGAGAACGGCACTCTCCCGATGACGTCGCCAAGCTCGTGGTTTGCATTCAGCTTTTCGCGATGAGCTTGTGGAACTATGGGCCTGATGTGATGGATGCATTGATTGAGTTGATTATTTCCTTGGCTGCTTCAAGTGGGAAGTATCTTGATCTATGCTTGGCTATGCTTACACGCCATTTCGAGACGCCTTCAAATTTCTTGGAGAAGTTGAAGTTGCCGTCTGGCCTTACAAAGAAGGACCAAGTTTTAACTCGTGTGCATTCAGCTCTGAAAGATATAGCTGATGTGGTTCCGCTTTCCCCTGCAAGAATAGTGACTATAGTTTCTGATCGTCTAGAGCTATATCGGCAAATGAGGGGAACTAGTACACATGGTCTTGTGATATTCGTAGAGAACATGTTAAAGTTGGAGGGTGGTGCACTTGGTGAAGTTGTTCGAGTTTCCATGCTTATGAAGGTGGTGGAATTGCTACTACATTTGGATGTCGAAATGGGATCATGGGACAAAGTGTTACAAGATCATGGAATATTTCAGATGGAGCTAGAAGATGTGGACGACTTTACAGAAGATTTTCTGAATGATGGTACTGAGCATGCAAGAGAAGCAACTATAAACGACGTAACTTTGAAAAGTTTTGCTGAAAAGTTGGATACCTTGATGGTGTTAACTTTTGAGCACCTTGAATCCTGTGAAGCTGGTGGCCGGTTGAGTGAGGTCTTCAAAACTCTTCTCGAGACATTTAGGAACAAAGTTTTGACCACATGCAAGTCAAAATTTGTCCAGTTTGTGATGTTTTATGCCTGTGCTCTTGATCCTGTAAACTGTGGTGAGGAATTTGCCACAAACCTTGTCGAAATTTTTCTTTACGATGCCAACCTCACATGTCTCAGGATGAGAGCTGTTGCGTACCTTGCTAGCTACTTGTCTCGAGCGATGTTTATGTCCGCCTCCATAGTTGCTCAAACGCTAAAAAGGTTGGTGGGTTGGTGTTTTAATTATGTCAAAACAAAGAAAGGTGGAGTAAACCCAGAAAAAGATAAATTATTTTATGCTGCGTGTCAGGTCTTACATCTAATAGCTGAGGCCATCATGTATTTGCTGTGCTTCCGAATGAGATCTTTGATGGATGATCCTCAATCAAAGTTGTTGCTTGCTGGTCTACCTTTAGAGTCCATTTTGAGCACTGAAATTAGCCCATTGAAGGCGTGTGCGCCTTCCATAGTGAAGGAGTTCCTATGGCAAGCGAATGCAGCTGGCTTAATCACGGCTGAGGAATTTGATTTTGATGATGGTCTGGAATCCGAATATTCATGGGTATGGGGTGGAAAGGAAGAGCTTCACAAGTTGTTCCCTTTTGACCCTTGTTTGCTGAGAAATAGTGACAGATTCATACGGCCCAATTTCATATACTGGTCAATGGTTCAACGAACCTACGACGAGGAAAAAGAAGGTTCCAGCGATGAAGAAGGTGATGAAGCTTTAGTGGTTGACAATGAGGAGAACGTAATGGATTATGAGATTTTGCAATGCTTGGAGGAACAACAATTCGATGAAGTTGACAATGCATTAAACAAAATGTCCATCACGCCAAAGGATTCATTACACAATAGATTTGGTGGTGCAATAAATAAACCCATGCGAATGCCCTCACGAATTAGACCATCCACAAGTCCAGAGTCTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRN3

Protein Analysis

614

Amino Acids

69.48

Weight (kDa)

4.77

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRN3 PF05327 71 - 528 4.2e-93 RNA polymerase I specific transcription initiation factor RRN3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1843
AatII GACGTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 1318
AccII CGCG 1 cut(s) 218
AciI CCGC 3 cut(s) 42, 452, 1104
AclWI GGATC 3 cut(s) 661, 965, 1283
AcoI YGGCCR 1 cut(s) 859
AcsI RAATTY 5 cut(s) 352, 932, 992, 1014, 1433
AcyI GRCGYC 4 cut(s) 6, 148, 183, 344
AdeI CACNNNGTG 1 cut(s) 584
AfaI GTAC 4 cut(s) 155, 526, 745, 1067
AfiI CCNNNNNNNGG 2 cut(s) 31, 1360
AflIII ACRYGT 3 cut(s) 73, 555, 1040
AgsI TTSAA 8 cut(s) 291, 351, 370, 787, 842, 880, 1360, 1583
AhlI ACTAGT 1 cut(s) 521
AjiI CACGTC 1 cut(s) 76
Alw21I GWGCWC 6 cut(s) 15, 141, 580, 837, 967, 1343
Alw26I GTCTC 5 cut(s) 103, 335, 887, 1049, 1089
Alw44I GTGCAC 1 cut(s) 576
AlwI GGATC 3 cut(s) 661, 965, 1283
Ama87I CYCGRG 2 cut(s) 890, 1086
AoxI GGCC 5 cut(s) 239, 382, 859, 1242, 1553
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 3 cut(s) 284, 1211, 1409
ApoI RAATTY 5 cut(s) 352, 932, 992, 1014, 1433
Asp700I GAANNNNTTC 5 cut(s) 591, 729, 1017, 1388, 1494
AspLEI GCGC 1 cut(s) 1372
AspS9I GGNCC 3 cut(s) 239, 397, 1554
AsuHPI GGTGA 3 cut(s) 596, 998, 1640
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 890, 1086
AvaII GGWCC 1 cut(s) 397
BaeGI GKGCMC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 15, 141
BauI CACGAG 2 cut(s) 194, 411
BbsI GAAGAC 1 cut(s) 868
Bbv12I GWGCWC 6 cut(s) 15, 141, 580, 837, 967, 1343
BbvCI CCTCAGC 2 cut(s) 1239, 1428
BbvI GCAGC 3 cut(s) 271, 1198, 1421
BccI CCATC 9 cut(s) 244, 688, 734, 811, 1253, 1276, 1442, 1748, 1828
BceAI ACGGC 5 cut(s) 55, 182, 361, 1440, 1568
BciVI GTATCC 1 cut(s) 802
BcoDI GTCTC 5 cut(s) 103, 335, 887, 1049, 1089
BcuI ACTAGT 1 cut(s) 521
BfaI CTAG 6 cut(s) 65, 159, 494, 522, 701, 1074
BfmI CTRYAG 2 cut(s) 130, 477
BfuI GTATCC 1 cut(s) 802
BglII AGATCT 1 cut(s) 1274
BisI GCNGC 4 cut(s) 43, 285, 1212, 1410
BlsI GCNGC 4 cut(s) 44, 286, 1213, 1411
Bme18I GGWCC 1 cut(s) 397
BmeT110I CYCGRG 2 cut(s) 890, 1086
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 3 cut(s) 239, 397, 1554
BmiI GGNNCC 2 cut(s) 450, 1612
BmsI GCATC 2 cut(s) 244, 1018
BmtI GCTAGC 1 cut(s) 1077
BpiI GAAGAC 1 cut(s) 868
Bpu10I CCTNAGC 2 cut(s) 1239, 1428
BsaHI GRCGYC 4 cut(s) 6, 148, 183, 344
BsaJI CCNNGG 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 31, 1360
Bse118I RCCGGY 1 cut(s) 861
Bse1I ACTGG 2 cut(s) 940, 1574
Bse3DI GCAATG 1 cut(s) 1691
BseDI CCNNGG 1 cut(s) 279
BseGI GGATG 6 cut(s) 88, 259, 646, 1056, 1291, 1820
BseLI CCNNNNNNNGG 2 cut(s) 31, 1360
BseMI GCAATG 1 cut(s) 1691
BseMII CTCAG 6 cut(s) 108, 738, 1060, 1230, 1419, 1520
BseNI ACTGG 2 cut(s) 940, 1574
BseRI GAGGAG 3 cut(s) 80, 91, 1670
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 3 cut(s) 271, 1198, 1421
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 5 cut(s) 241, 384, 861, 1244, 1555
BsiHKAI GWGCWC 6 cut(s) 15, 141, 580, 837, 967, 1343
BsiHKCI CYCGRG 2 cut(s) 890, 1086
BsiSI CCGG 2 cut(s) 16, 862
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 2 cut(s) 31, 1360
BsmAI GTCTC 5 cut(s) 103, 335, 887, 1049, 1089
BsmBI CGTCTC 1 cut(s) 335
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 4 cut(s) 204, 418, 1411, 1806
BsnI GGCC 5 cut(s) 241, 384, 861, 1244, 1555
BsoBI CYCGRG 2 cut(s) 890, 1086
Bsp1286I GDGCHC 6 cut(s) 15, 141, 580, 837, 967, 1343
Bsp143I GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
Bsp68I TCGCGA 1 cut(s) 218
BspACI CCGC 3 cut(s) 42, 452, 1104
BspANI GGCC 5 cut(s) 241, 384, 861, 1244, 1555
BspCNI CTCAG 6 cut(s) 107, 739, 1059, 1231, 1420, 1521
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 2 cut(s) 450, 1612
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 3 cut(s) 661, 965, 1283
BspQI GCTCTTC 1 cut(s) 1485
BsrDI GCAATG 1 cut(s) 1691
BsrFI RCCGGY 1 cut(s) 861
BsrI ACTGG 2 cut(s) 940, 1574
BssAI RCCGGY 1 cut(s) 861
BssECI CCNNGG 1 cut(s) 279
BssMI GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
BssNI GRCGYC 4 cut(s) 6, 148, 183, 344
BssSI CACGAG 2 cut(s) 194, 411
BssT1I CCWWGG 1 cut(s) 279
Bst2BI CACGAG 2 cut(s) 194, 411
Bst4CI ACNGT 1 cut(s) 983
Bst6I CTCTTC 2 cut(s) 891, 1485
BstACI GRCGYC 4 cut(s) 6, 148, 183, 344
BstC8I GCNNGC 5 cut(s) 753, 1075, 1311, 1401, 1414
BstDEI CTNAG 6 cut(s) 94, 747, 1046, 1239, 1428, 1529
BstF5I GGATG 6 cut(s) 88, 259, 646, 1056, 1291, 1820
BstFNI CGCG 1 cut(s) 218
BstHHI GCGC 1 cut(s) 1372
BstKTI GATC 7 cut(s) 310, 490, 656, 679, 973, 1277, 1291
BstMAI GTCTC 5 cut(s) 103, 335, 887, 1049, 1089
BstMBI GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
BstMWI GCNNNNNNNGC 5 cut(s) 145, 505, 761, 1070, 1409
BstNSI RCATGY 4 cut(s) 559, 755, 924, 1044
BstSFI CTRYAG 2 cut(s) 130, 477
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 3 cut(s) 271, 1198, 1421
BstV2I GAAGAC 1 cut(s) 868
BstX2I RGATCY 1 cut(s) 1274
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 1274
BsuI GTATCC 1 cut(s) 802
BsuRI GGCC 5 cut(s) 241, 384, 861, 1244, 1555
BtgZI GCGATG 4 cut(s) 123, 233, 1106, 1632
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 6 cut(s) 88, 259, 646, 1056, 1291, 1820
BtsIMutI CAGTG 1 cut(s) 1341
BtuMI TCGCGA 1 cut(s) 218
Cac8I GCNNGC 5 cut(s) 753, 1075, 1311, 1401, 1414
CfoI GCGC 1 cut(s) 1372
Cfr10I RCCGGY 1 cut(s) 861
Cfr13I GGNCC 3 cut(s) 239, 397, 1554
CseI GACGC 2 cut(s) 137, 352
Csp6I GTAC 4 cut(s) 154, 525, 744, 1066
CspCI CAANNNNNGTGG 2 cut(s) 988, 1023
CviQI GTAC 4 cut(s) 154, 525, 744, 1066
DdeI CTNAG 6 cut(s) 94, 747, 1046, 1239, 1428, 1529
DpnI GATC 7 cut(s) 309, 489, 655, 678, 972, 1276, 1290
DpnII GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
DraIII CACNNNGTG 1 cut(s) 584
EaeI YGGCCR 1 cut(s) 859
Eam1104I CTCTTC 2 cut(s) 891, 1485
EarI CTCTTC 2 cut(s) 891, 1485
EciI GGCGGA 1 cut(s) 1093
Ecl136II GAGCTC 2 cut(s) 13, 139
Eco130I CCWWGG 1 cut(s) 279
Eco24I GRGCYC 2 cut(s) 15, 141
Eco47I GGWCC 1 cut(s) 397
Eco53kI GAGCTC 2 cut(s) 13, 139
Eco88I CYCGRG 2 cut(s) 890, 1086
EcoICRI GAGCTC 2 cut(s) 13, 139
EcoT14I CCWWGG 1 cut(s) 279
EcoT22I ATGCAT 2 cut(s) 259, 1726
EcoT38I GRGCYC 2 cut(s) 15, 141
ErhI CCWWGG 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 335
FaqI GGGAC 1 cut(s) 674
FblI GTMKAC 1 cut(s) 1318
Fnu4HI GCNGC 4 cut(s) 43, 285, 1212, 1410
FokI GGATG 6 cut(s) 95, 266, 653, 1063, 1298, 1807
FriOI GRGCYC 2 cut(s) 15, 141
Fsp4HI GCNGC 4 cut(s) 43, 285, 1212, 1410
FspBI CTAG 6 cut(s) 65, 159, 494, 522, 701, 1074
GlaI GCGC 1 cut(s) 1371
GluI GCNGC 4 cut(s) 43, 285, 1212, 1410
HaeIII GGCC 5 cut(s) 241, 384, 861, 1244, 1555
HapII CCGG 2 cut(s) 16, 862
HgaI GACGC 2 cut(s) 137, 352
HhaI GCGC 1 cut(s) 1372
Hin1I GRCGYC 4 cut(s) 6, 148, 183, 344
Hin6I GCGC 1 cut(s) 1370
HinP1I GCGC 1 cut(s) 1370
HincII GTYRAC 3 cut(s) 825, 1648, 1717
HindII GTYRAC 3 cut(s) 825, 1648, 1717
HindIII AAGCTT 1 cut(s) 1635
HinfI GANTC 6 cut(s) 842, 1328, 1457, 1545, 1754, 1835
HpaI GTTAAC 1 cut(s) 825
HpaII CCGG 2 cut(s) 16, 862
HphI GGTGA 3 cut(s) 596, 998, 1640
Hpy188I TCNGA 7 cut(s) 55, 429, 487, 693, 735, 1268, 1462
Hpy99I CGWCG 6 cut(s) 11, 41, 146, 188, 779, 1598
HpyAV CCTTC 8 cut(s) 357, 388, 607, 1354, 1378, 1383, 1601, 1619
HpyCH4III ACNGT 1 cut(s) 983
HpyCH4IV ACGT 4 cut(s) 75, 183, 777, 1662
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 505, 761, 1070, 1409
HpyF3I CTNAG 6 cut(s) 94, 747, 1046, 1239, 1428, 1529
HpySE526I ACGT 4 cut(s) 75, 183, 777, 1662
Hsp92I GRCGYC 4 cut(s) 6, 148, 183, 344
HspAI GCGC 1 cut(s) 1370
KspAI GTTAAC 1 cut(s) 825
Kzo9I GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
LguI GCTCTTC 1 cut(s) 1485
LmnI GCTCC 2 cut(s) 18, 697
Lsp1109I GCAGC 3 cut(s) 271, 1198, 1421
LweI GCATC 2 cut(s) 244, 1018
MaeI CTAG 6 cut(s) 65, 159, 494, 522, 701, 1074
MaeII ACGT 4 cut(s) 75, 183, 777, 1662
MaeIII GTNAC 4 cut(s) 472, 669, 778, 1538
MalI GATC 7 cut(s) 309, 489, 655, 678, 972, 1276, 1290
MboI GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
MboII GAAGA 6 cut(s) 716, 737, 868, 878, 1502, 1634
MflI RGATCY 1 cut(s) 1274
MhlI GDGCHC 6 cut(s) 15, 141, 580, 837, 967, 1343
MlyI GAGTC 2 cut(s) 1337, 1844
MmeI TCCRAC 3 cut(s) 78, 546, 786
Mph1103I ATGCAT 2 cut(s) 259, 1726
MroXI GAANNNNTTC 5 cut(s) 591, 729, 1017, 1388, 1494
MseI TTAA 6 cut(s) 407, 560, 824, 1152, 1418, 1727
MslI CAYNNNNRTG 4 cut(s) 608, 639, 1474, 1799
MspA1I CMGCKG 1 cut(s) 1412
MspI CCGG 2 cut(s) 16, 862
Mva1269I GAATGC 4 cut(s) 204, 418, 1411, 1806
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 5 cut(s) 145, 505, 761, 1070, 1409
NdeII GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
NheI GCTAGC 1 cut(s) 1073
NlaIV GGNNCC 2 cut(s) 450, 1612
NmuCI GTSAC 2 cut(s) 472, 1538
NruI TCGCGA 1 cut(s) 218
NsiI ATGCAT 2 cut(s) 259, 1726
NspI RCATGY 4 cut(s) 559, 755, 924, 1044
PaeI GCATGC 1 cut(s) 755
PaeR7I CTCGAG 2 cut(s) 890, 1086
PciI ACATGT 2 cut(s) 555, 1040
PciSI GCTCTTC 1 cut(s) 1485
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 192
PctI GAATGC 4 cut(s) 204, 418, 1411, 1806
PdmI GAANNNNTTC 5 cut(s) 591, 729, 1017, 1388, 1494
PfeI GAWTC 4 cut(s) 842, 1457, 1545, 1754
PkrI GCNGC 4 cut(s) 44, 286, 1213, 1411
PleI GAGTC 2 cut(s) 1336, 1843
PpsI GAGTC 2 cut(s) 1336, 1843
PscI ACATGT 2 cut(s) 555, 1040
PsiI TTATAA 1 cut(s) 1843
Psp124BI GAGCTC 2 cut(s) 15, 141
PspN4I GGNNCC 2 cut(s) 450, 1612
PspPI GGNCC 3 cut(s) 239, 397, 1554
PsuI RGATCY 1 cut(s) 1274
PvuII CAGCTG 1 cut(s) 1412
RruI TCGCGA 1 cut(s) 218
RsaI GTAC 4 cut(s) 155, 526, 745, 1067
RsaNI GTAC 4 cut(s) 154, 525, 744, 1066
RseI CAYNNNNRTG 4 cut(s) 608, 639, 1474, 1799
SacI GAGCTC 2 cut(s) 15, 141
SapI GCTCTTC 1 cut(s) 1485
SaqAI TTAA 6 cut(s) 407, 560, 824, 1152, 1418, 1727
SatI GCNGC 4 cut(s) 43, 285, 1212, 1410
Sau3AI GATC 7 cut(s) 307, 487, 653, 676, 970, 1274, 1288
Sau96I GGNCC 3 cut(s) 239, 397, 1554
SchI GAGTC 2 cut(s) 1337, 1844
SduI GDGCHC 6 cut(s) 15, 141, 580, 837, 967, 1343
SfaNI GCATC 2 cut(s) 244, 1018
SfcI CTRYAG 2 cut(s) 130, 477
Sfr274I CTCGAG 2 cut(s) 890, 1086
SinI GGWCC 1 cut(s) 397
SlaI CTCGAG 2 cut(s) 890, 1086
SmiMI CAYNNNNRTG 4 cut(s) 608, 639, 1474, 1799
SmlI CTYRAG 2 cut(s) 890, 1086
SmoI CTYRAG 2 cut(s) 890, 1086
SpeI ACTAGT 1 cut(s) 521
SphI GCATGC 1 cut(s) 755
SsiI CCGC 3 cut(s) 42, 452, 1104
SspI AATATT 2 cut(s) 687, 1466
SspMI CTAG 6 cut(s) 65, 159, 494, 522, 701, 1074
SstI GAGCTC 2 cut(s) 15, 141
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 983
TaiI ACGT 4 cut(s) 78, 186, 780, 1665
TaqI TCGA 8 cut(s) 9, 339, 595, 645, 891, 1011, 1087, 1707
TatI WGTACW 1 cut(s) 524
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 4 cut(s) 842, 1457, 1545, 1754
Tru1I TTAA 6 cut(s) 407, 560, 824, 1152, 1418, 1727
Tru9I TTAA 6 cut(s) 407, 560, 824, 1152, 1418, 1727
TscAI CASTG 1 cut(s) 1348
TseFI GTSAC 2 cut(s) 472, 1538
TseI GCWGC 3 cut(s) 284, 1211, 1409
Tsp45I GTSAC 2 cut(s) 472, 1538
TspDTI ATGAA 8 cut(s) 626, 1458, 1537, 1552, 1635, 1647, 1725, 1746
TspRI CASTG 1 cut(s) 1348
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 1 cut(s) 397
XapI RAATTY 5 cut(s) 352, 932, 992, 1014, 1433
XbaI TCTAGA 1 cut(s) 493
XceI RCATGY 4 cut(s) 559, 755, 924, 1044
XhoI CTCGAG 2 cut(s) 890, 1086
XmiI GTMKAC 1 cut(s) 1318
XmnI GAANNNNTTC 5 cut(s) 591, 729, 1017, 1388, 1494
XspI CTAG 6 cut(s) 65, 159, 494, 522, 701, 1074
ZraI GACGTC 1 cut(s) 184
Zsp2I ATGCAT 2 cut(s) 259, 1726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.