AT1G32340

RWD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008811
Physical Location & Seq
Reverse (-)
11663008 .. 11666120
3113 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G32340.1

Sequence Viewer

Length: 2067 bp
ATGAGAGGAACACACGGAGGCAGTGGAAGAAATCACGACCGCCGCTACATCCGCCGTGACGCTGGGATTGTGAATCCAGTCAACGATCATCCAAAATCAGAGCATCAGAAAGAAGACACGACTTCGACGGATTTATCATCTGCAGCAGCTTTTCCGTCTACTTCTCAGCCAATCCAAAACCTCGATCATTCTACAAAACCTTCAAAGTCTCACCGGAATCGACGCAGCCGTGGATCAAATTCAAAACCTCGTCCCGTGGAGAAACCGGAGGTGAATTTTGCTGAATCCGATGTTGTTGATTGCTTGGCTGATGAATTGAGTAGGTTAAAGGTGAAACAGAATTCGCATTCGGTGAATATGGAGGAGAAGTTTCATCACAGTTCTAGTGATCATTCGAATTGTGAGGAATCGGAGTTAAAGAGAGCTGAAAGTGAAGAAATTGCTGATGGTGTTGATGAATATGAGACGAAGGAAGATATTATGTTGACAATTCTGAAAGATTTGCGATCTAGTGTCAGTGAGCCAGAGCTAACAGAGGAGCAACTAAAGATGAATGATCAATTACAGGAAGATGAGCTTTTGGCATTGGGGTACATATATGGAGGAAACATGTTCATCTTTGATCGGTATAACGATATGCGATACTTTCAGGTTCATGTAAACGTTGAAGCTACTAGTGAATATACTATCTCTACAAAGCTTAACTTACAAGCTGATTCAAGCAAAGAGTCAGAGGATTTCTTGTACTCTTTCAAGGCGCAACACCTTCCACCGATTGTGTTGAAATGTTTATTACCAAAAGCTTATCCGAGCCACTTACCGCCTTATTTCTTAATAAGTGTTCAGTGGATGAATCCTGATAAGATTTCAAGTCTCTGCTCTAAGCTGGACTCGTTATGGAGTGAACAGCCAGGGCAAGAAGTCTTGTACCAGTGGACAGATTGGCTACAGAACTCGTCGATTTCTCATCTTGGTTTTGATAATGAGATTGTTCTTGGTCCTTATGGTGTTACATGTTCCAGAGATAAGCGTGCTGTGTCTGGAAGTCGTTCTCCGGATTCAGATATTCCTTATATTAGGAGTTATGACGATGAGAAACGTCATGATAGCTTCCTTCAGAGCTTGCATGAGTGCTGCATATGCTTCTCTGAATCTGCAGGTATCGACTTTGTTAAGTTACCATGCCAGCATTTCTTTTGTTTGAAATGTATGAAGACCTATACAGACATACATGTCACTGAAGGCACAGTTAACAAGCTCAAGTGTCCGGACTCGAAATGTGGAGAAACTGTTCCGCCAGGGATATTGAAGAGATTGTTGGGCGATGAAGCGTATGAACGGTGGGAAACTCTTATGTTACAGAAAACACTGGAATCCATGACTGACGTTGCTTACTGTCCTAGATGTGAAACCCCTTGCATAGAGGACGAGGAACAGCTTGCTTTATGCTTCAAATGCTACTTCAGTTTCTGTACACTTTGTAAGGAAAAGAGACATGTGGGTGTGGCTTGCATGAGCCCGGAACTTAGGCTTCAAATCTTGCAGGAGCGTCAAGATTCTTCTCGTCTTGGAGAGGAACAAAGGCGGAAAGAGAAAGAAATGATCAATGAGATTATGAGTGTGAAGGTGATAATGAAAAGCGCTAAACAATGTCCGTCGTGCAAAATAGCCATCTCAAGAACCGGAGGTTGTAACAAAATGGTATGCAATAACTGTGGTCAATACTTTTGTTACCGTTGCAACGAAGCTATCACTGGTTATGAACATTTCAGTGAGGGAAAATGTGAACTGTTCCCACAAGAGGCGATCCAAGAATGGAATGAGATGATGAATGAGAGACAAGTCATTGGACAGATTCAAGCGCAACTGTTTGCACAAGGCGGTCAGTTTCCACAGCGCGGTCAGTTATGTCCTAATTGCCGCCAGTTCAATGGAAAGGCGGGAAACAACAATCACTTGTTTTGTTGGGCATGTCAAGCACATTTCTGTTATCTTTGTAAGAAAGTGGTGAAGAAATCTGCTCAGCATTATGGACCAAAGGGCTGCAAGCAGCACACAGATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0023051 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033143 GO:0035257 GO:0035258 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044444 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051427 GO:0051716 GO:0060255 GO:0060765 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1990234 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RWD zf-C3HC4

Protein Analysis

688

Amino Acids

78.6

Weight (kDa)

5.98

Isoelectric Point (pI)

53.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 187 - 319 4e-22 RWD domain
IBR PF01485 446 - 504 4.5e-08 IBR domain, a half RING-finger domain
IBR PF01485 545 - 583 2e-06 IBR domain, a half RING-finger domain
Rcat_RNF216 PF26200 547 - 592 9e-09 RNF216 Rcat domain
IBR_1 PF22191 548 - 582 2.4e-07 IBR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1232
Acc36I ACCTGC 1 cut(s) 1148
AccI GTMKAC 1 cut(s) 158
AccII CGCG 1 cut(s) 1899
AccIII TCCGGA 2 cut(s) 1054, 1267
AclI AACGTT 1 cut(s) 663
AclWI GGATC 2 cut(s) 241, 1801
AcsI RAATTY 3 cut(s) 238, 274, 340
AcuI CTGAAG 3 cut(s) 1100, 1260, 1447
AfaI GTAC 4 cut(s) 593, 746, 929, 1474
AfeI AGCGCT 1 cut(s) 1642
AfiI CCNNNNNNNGG 2 cut(s) 1801, 1898
AflIII ACRYGT 4 cut(s) 609, 1013, 1231, 1495
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 2 cut(s) 910, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1301, 1333
AloI GAACNNNNNNTCC 2 cut(s) 1275, 1307
Alw26I GTCTC 5 cut(s) 213, 458, 878, 1486, 1831
AlwI GGATC 2 cut(s) 241, 1801
AlwNI CAGNNNCTG 1 cut(s) 1470
Aor13HI TCCGGA 2 cut(s) 1054, 1267
Aor51HI AGCGCT 1 cut(s) 1642
ApeKI GCWGC 6 cut(s) 143, 146, 225, 1134, 2043, 2050
ApoI RAATTY 3 cut(s) 238, 274, 340
Asp700I GAANNNNTTC 2 cut(s) 1048, 1290
AspLEI GCGC 4 cut(s) 760, 1643, 1864, 1899
AspS9I GGNCC 2 cut(s) 998, 2033
AsuC2I CCSGG 1 cut(s) 1520
AsuHPI GGTGA 6 cut(s) 203, 283, 343, 364, 1639, 2020
AsuII TTCGAA 1 cut(s) 395
AvaII GGWCC 2 cut(s) 998, 2033
BanII GRGCYC 1 cut(s) 1520
BbsI GAAGAC 2 cut(s) 120, 1220
BbvI GCAGC 6 cut(s) 155, 158, 237, 1121, 2030, 2062
BccI CCATC 3 cut(s) 440, 1679, 2054
BceAI ACGGC 2 cut(s) 39, 213
BciT130I CCWGG 2 cut(s) 912, 1299
BclI TGATCA 3 cut(s) 388, 556, 1602
BcnI CCSGG 1 cut(s) 1520
BcoDI GTCTC 5 cut(s) 213, 458, 878, 1486, 1831
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 4 cut(s) 384, 510, 675, 1401
BfmI CTRYAG 3 cut(s) 141, 947, 1155
BfoI RGCGCY 1 cut(s) 1644
BfuAI ACCTGC 1 cut(s) 1148
BisI GCNGC 8 cut(s) 43, 144, 147, 226, 1135, 1921, 2044, 2051
BlpI GCTNAGC 1 cut(s) 2022
BlsI GCNGC 8 cut(s) 44, 145, 148, 227, 1136, 1922, 2045, 2052
Bme1390I CCNGG 3 cut(s) 912, 1299, 1520
Bme18I GGWCC 2 cut(s) 998, 2033
BmgT120I GGNCC 2 cut(s) 998, 2033
BmrFI CCNGG 3 cut(s) 912, 1299, 1520
BmsI GCATC 1 cut(s) 112
BpiI GAAGAC 2 cut(s) 120, 1220
Bpu1102I GCTNAGC 1 cut(s) 2022
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 1244, 1660
BpuMI CCSGG 1 cut(s) 1520
BsaJI CCNNGG 4 cut(s) 229, 255, 911, 1298
BsaWI WCCGGW 5 cut(s) 213, 265, 1054, 1267, 1682
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 1275, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 1801, 1898
Bse1I ACTGG 5 cut(s) 77, 931, 1374, 1759, 1924
BseAI TCCGGA 2 cut(s) 1054, 1267
BseBI CCWGG 2 cut(s) 912, 1299
BseDI CCNNGG 4 cut(s) 229, 255, 911, 1298
BseGI GGATG 3 cut(s) 48, 88, 855
BseLI CCNNNNNNNGG 2 cut(s) 1801, 1898
BseMII CTCAG 2 cut(s) 179, 2036
BseNI ACTGG 5 cut(s) 77, 931, 1374, 1759, 1924
BseRI GAGGAG 2 cut(s) 377, 551
BseXI GCAGC 6 cut(s) 155, 158, 237, 1121, 2030, 2062
BseYI CCCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 1899
Bsh1285I CGRYCG 1 cut(s) 40
BsiEI CGRYCG 1 cut(s) 40
BsiSI CCGG 6 cut(s) 214, 266, 1055, 1268, 1520, 1683
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 1801, 1898
BsmAI GTCTC 5 cut(s) 213, 458, 878, 1486, 1831
BsmBI CGTCTC 1 cut(s) 458
BsmFI GGGAC 1 cut(s) 237
BsmI GAATGC 1 cut(s) 346
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 1520
Bsp13I TCCGGA 2 cut(s) 1054, 1267
Bsp1407I TGTACA 1 cut(s) 1472
Bsp143I GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
Bsp1720I GCTNAGC 1 cut(s) 2022
BspCNI CTCAG 2 cut(s) 178, 2035
BspEI TCCGGA 2 cut(s) 1054, 1267
BspFNI CGCG 1 cut(s) 1899
BspHI TCATGA 1 cut(s) 1102
BspMAI CTGCAG 2 cut(s) 145, 1159
BspMI ACCTGC 1 cut(s) 1148
BspPI GGATC 2 cut(s) 241, 1801
BspT104I TTCGAA 1 cut(s) 395
BsrGI TGTACA 1 cut(s) 1472
BsrI ACTGG 5 cut(s) 77, 931, 1374, 1759, 1924
BssECI CCNNGG 4 cut(s) 229, 255, 911, 1298
BssMI GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
Bst2UI CCWGG 2 cut(s) 912, 1299
Bst4CI ACNGT 9 cut(s) 380, 1249, 1291, 1341, 1397, 1715, 1736, 1791, 1869
Bst6I CTCTTC 1 cut(s) 1304
BstAUI TGTACA 1 cut(s) 1472
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 6 cut(s) 1032, 1124, 1187, 1440, 1510, 2048
BstDEI CTNAG 4 cut(s) 165, 882, 1526, 2022
BstDSI CCRYGG 2 cut(s) 229, 255
BstF5I GGATG 3 cut(s) 48, 88, 855
BstFNI CGCG 1 cut(s) 1899
BstH2I RGCGCY 1 cut(s) 1644
BstHHI GCGC 4 cut(s) 760, 1643, 1864, 1899
BstKTI GATC 9 cut(s) 88, 187, 236, 391, 509, 559, 625, 1605, 1809
BstMAI GTCTC 5 cut(s) 213, 458, 878, 1486, 1831
BstMBI GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 4 cut(s) 51, 1140, 1455, 1976
BstNI CCWGG 2 cut(s) 912, 1299
BstNSI RCATGY 5 cut(s) 613, 1017, 1235, 1499, 1974
BstSCI CCNGG 3 cut(s) 910, 1297, 1518
BstSFI CTRYAG 3 cut(s) 141, 947, 1155
BstUI CGCG 1 cut(s) 1899
BstV1I GCAGC 6 cut(s) 155, 158, 237, 1121, 2030, 2062
BstV2I GAAGAC 2 cut(s) 120, 1220
BstXI CCANNNNNNTGG 1 cut(s) 1931
BtgI CCRYGG 2 cut(s) 229, 255
BtgZI GCGATG 1 cut(s) 1338
BtsCI GGATG 3 cut(s) 48, 88, 855
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 8 cut(s) 28, 523, 851, 938, 1236, 1367, 1752, 1777
BveI ACCTGC 1 cut(s) 1148
Cac8I GCNNGC 6 cut(s) 1032, 1124, 1187, 1440, 1510, 2048
CaiI CAGNNNCTG 1 cut(s) 1470
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 4 cut(s) 760, 1643, 1864, 1899
Cfr13I GGNCC 2 cut(s) 998, 2033
CseI GACGC 3 cut(s) 68, 231, 1538
Csp6I GTAC 4 cut(s) 592, 745, 928, 1473
CspCI CAANNNNNGTGG 2 cut(s) 1696, 1731
CviQI GTAC 4 cut(s) 592, 745, 928, 1473
DdeI CTNAG 4 cut(s) 165, 882, 1526, 2022
DpnI GATC 9 cut(s) 87, 186, 235, 390, 508, 558, 624, 1604, 1808
DpnII GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
DrdI GACNNNNNNGTC 1 cut(s) 1232
DseDI GACNNNNNNGTC 1 cut(s) 1232
Eam1104I CTCTTC 1 cut(s) 1304
EarI CTCTTC 1 cut(s) 1304
EciI GGCGGA 3 cut(s) 41, 1284, 1600
Eco24I GRGCYC 1 cut(s) 1520
Eco47I GGWCC 2 cut(s) 998, 2033
Eco47III AGCGCT 1 cut(s) 1642
Eco57I CTGAAG 3 cut(s) 1100, 1260, 1447
EcoRI GAATTC 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 910, 1297
EcoT38I GRGCYC 1 cut(s) 1520
Esp3I CGTCTC 1 cut(s) 458
FalI AAGNNNNNCTT 4 cut(s) 561, 593, 689, 721
FaqI GGGAC 1 cut(s) 237
FauI CCCGC 1 cut(s) 1933
FauNDI CATATG 1 cut(s) 1139
FbaI TGATCA 3 cut(s) 388, 556, 1602
FblI GTMKAC 1 cut(s) 158
Fnu4HI GCNGC 8 cut(s) 43, 144, 147, 226, 1135, 1921, 2044, 2051
FokI GGATG 3 cut(s) 35, 75, 862
FriOI GRGCYC 1 cut(s) 1520
Fsp4HI GCNGC 8 cut(s) 43, 144, 147, 226, 1135, 1921, 2044, 2051
FspBI CTAG 4 cut(s) 384, 510, 675, 1401
GlaI GCGC 4 cut(s) 759, 1642, 1863, 1898
GluI GCNGC 8 cut(s) 43, 144, 147, 226, 1135, 1921, 2044, 2051
GsaI CCCAGC 1 cut(s) 66
HaeII RGCGCY 1 cut(s) 1644
HapII CCGG 6 cut(s) 214, 266, 1055, 1268, 1520, 1683
HgaI GACGC 3 cut(s) 68, 231, 1538
HhaI GCGC 4 cut(s) 760, 1643, 1864, 1899
Hin6I GCGC 4 cut(s) 758, 1641, 1862, 1897
HinP1I GCGC 4 cut(s) 758, 1641, 1862, 1897
HincII GTYRAC 3 cut(s) 82, 486, 1252
HindII GTYRAC 3 cut(s) 82, 486, 1252
HindIII AAGCTT 2 cut(s) 698, 801
HpaI GTTAAC 1 cut(s) 1252
HpaII CCGG 6 cut(s) 214, 266, 1055, 1268, 1520, 1683
HphI GGTGA 6 cut(s) 203, 283, 343, 364, 1639, 2020
Hpy166II GTNNAC 9 cut(s) 82, 159, 486, 661, 905, 936, 1252, 1475, 1787
Hpy188III TCNNGA 9 cut(s) 35, 857, 1020, 1041, 1055, 1103, 1268, 1553, 1677
Hpy8I GTNNAC 9 cut(s) 82, 159, 486, 661, 905, 936, 1252, 1475, 1787
Hpy99I CGWCG 4 cut(s) 130, 225, 961, 1660
HpyAV CCTTC 6 cut(s) 210, 463, 776, 1124, 1235, 1618
HpyCH4III ACNGT 9 cut(s) 380, 1249, 1291, 1341, 1397, 1715, 1736, 1791, 1869
HpyCH4IV ACGT 3 cut(s) 663, 1099, 1386
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 1140, 1455, 1976
HpyF3I CTNAG 4 cut(s) 165, 882, 1526, 2022
HpySE526I ACGT 3 cut(s) 663, 1099, 1386
HspAI GCGC 4 cut(s) 758, 1641, 1862, 1897
Kpn2I TCCGGA 2 cut(s) 1054, 1267
Ksp22I TGATCA 3 cut(s) 388, 556, 1602
KspAI GTTAAC 1 cut(s) 1252
Kzo9I GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
LmnI GCTCC 2 cut(s) 538, 1546
Lsp1109I GCAGC 6 cut(s) 155, 158, 237, 1121, 2030, 2062
LweI GCATC 1 cut(s) 112
MaeI CTAG 4 cut(s) 384, 510, 675, 1401
MaeII ACGT 3 cut(s) 663, 1099, 1386
MaeIII GTNAC 7 cut(s) 56, 1009, 1176, 1234, 1356, 1691, 1730
MalI GATC 9 cut(s) 87, 186, 235, 390, 508, 558, 624, 1604, 1808
MboI GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
MboII GAAGA 9 cut(s) 39, 125, 446, 485, 581, 1225, 1321, 1551, 2023
MhlI GDGCHC 1 cut(s) 1520
MluCI AATT 9 cut(s) 238, 274, 314, 340, 397, 438, 489, 560, 1915
MlyI GAGTC 3 cut(s) 737, 884, 1265
MroI TCCGGA 2 cut(s) 1054, 1267
MroXI GAANNNNTTC 2 cut(s) 1048, 1290
MseI TTAA 6 cut(s) 326, 416, 702, 833, 1173, 1251
MslI CAYNNNNRTG 3 cut(s) 1500, 1770, 2058
MspI CCGG 6 cut(s) 214, 266, 1055, 1268, 1520, 1683
MspR9I CCNGG 3 cut(s) 912, 1299, 1520
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 2 cut(s) 912, 1299
MvnI CGCG 1 cut(s) 1899
MwoI GCNNNNNNNGC 4 cut(s) 51, 1140, 1455, 1976
NciI CCSGG 1 cut(s) 1520
NdeI CATATG 1 cut(s) 1139
NdeII GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
NmuCI GTSAC 2 cut(s) 56, 1234
NspI RCATGY 5 cut(s) 613, 1017, 1235, 1499, 1974
NspV TTCGAA 1 cut(s) 395
PagI TCATGA 1 cut(s) 1102
PciI ACATGT 4 cut(s) 609, 1013, 1231, 1495
PcsI WCGNNNNNNNCGW 1 cut(s) 226
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 1048, 1290
PkrI GCNGC 8 cut(s) 44, 145, 148, 227, 1136, 1922, 2045, 2052
PleI GAGTC 3 cut(s) 736, 884, 1265
PpsI GAGTC 3 cut(s) 736, 884, 1265
PscI ACATGT 4 cut(s) 609, 1013, 1231, 1495
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 910, 1297
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 2 cut(s) 910, 1297
PspPI GGNCC 2 cut(s) 998, 2033
PstI CTGCAG 2 cut(s) 145, 1159
PstNI CAGNNNCTG 1 cut(s) 1470
RsaI GTAC 4 cut(s) 593, 746, 929, 1474
RsaNI GTAC 4 cut(s) 592, 745, 928, 1473
RseI CAYNNNNRTG 3 cut(s) 1500, 1770, 2058
SaqAI TTAA 6 cut(s) 326, 416, 702, 833, 1173, 1251
SatI GCNGC 8 cut(s) 43, 144, 147, 226, 1135, 1921, 2044, 2051
Sau3AI GATC 9 cut(s) 85, 184, 233, 388, 506, 556, 622, 1602, 1806
Sau96I GGNCC 2 cut(s) 998, 2033
SchI GAGTC 3 cut(s) 737, 884, 1265
ScrFI CCNGG 3 cut(s) 912, 1299, 1520
SduI GDGCHC 1 cut(s) 1520
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 3 cut(s) 141, 947, 1155
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 2 cut(s) 998, 2033
SmiMI CAYNNNNRTG 3 cut(s) 1500, 1770, 2058
SmlI CTYRAG 2 cut(s) 1259, 1675
SmoI CTYRAG 2 cut(s) 1259, 1675
SpeI ACTAGT 1 cut(s) 674
Sse9I AATT 9 cut(s) 238, 274, 314, 340, 397, 438, 489, 560, 1915
SspMI CTAG 4 cut(s) 384, 510, 675, 1401
StyD4I CCNGG 3 cut(s) 910, 1297, 1518
TaaI ACNGT 9 cut(s) 380, 1249, 1291, 1341, 1397, 1715, 1736, 1791, 1869
TaiI ACGT 3 cut(s) 666, 1102, 1389
TaqI TCGA 7 cut(s) 125, 183, 220, 395, 959, 1164, 1274
TasI AATT 9 cut(s) 238, 274, 314, 340, 397, 438, 489, 560, 1915
TatI WGTACW 2 cut(s) 744, 1472
TauI GCSGC 2 cut(s) 45, 1923
Tru1I TTAA 6 cut(s) 326, 416, 702, 833, 1173, 1251
Tru9I TTAA 6 cut(s) 326, 416, 702, 833, 1173, 1251
TscAI CASTG 8 cut(s) 28, 523, 851, 938, 1243, 1374, 1759, 1777
TseFI GTSAC 2 cut(s) 56, 1234
TseI GCWGC 6 cut(s) 143, 146, 225, 1134, 2043, 2050
Tsp45I GTSAC 2 cut(s) 56, 1234
TspGWI ACGGA 4 cut(s) 30, 143, 144, 1644
TspRI CASTG 8 cut(s) 28, 523, 851, 938, 1243, 1374, 1759, 1777
VpaK11BI GGWCC 2 cut(s) 998, 2033
XapI RAATTY 3 cut(s) 238, 274, 340
XceI RCATGY 5 cut(s) 613, 1017, 1235, 1499, 1974
XmiI GTMKAC 1 cut(s) 158
XmnI GAANNNNTTC 2 cut(s) 1048, 1290
XspI CTAG 4 cut(s) 384, 510, 675, 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.