Rroxscaffold_3G00248360

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008811
Physical Location & Seq
Reverse (-)
41657123 .. 41662952
5830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248360.1

Sequence Viewer

Length: 2070 bp
ATGAACGACGGAGGTAATAACCAAGCGGACGGATCTCCGGTGAGAAAGACCAGAAGAGGACGCGGAGGAGGAGGACGGGAACAACACCACCCTTCTCTCCAAATCGACGATGCATTAAAACTCAACGATCTTCAGTCACCTTTGACTTGTCAGCCTCAGACTCATGAACCCACCACCTCCACACCCAGAAAGCACAGAAATCCGAGGTCGGCATCCAGGAATCGAGGTGCCCATGTATTCAAACCTCGGTTTGTCAAGAAATCTGAGTTGGGTTCTTCTTCGAATGGAGTAGTGGCTTCTTCTTCTTCTTCGAATGAACAAGTGGGTTCTTTGAATGATGAGGTGGGGTCTCTGAGTATTATTGGTGAGGGAGAAAGTAGGCAGAGCGCGAAACAAGAAGAAGAGAGAGTTGGGGTTAGTGTAGAGAATGCTGCTCAGGTGTCTGAGCGTGGGAAAGAGGACGATGATGCTGTGACAAGATTGGAAGAGCTGCAGCGCGGTGTCGAAGAGCCGGAGTTGTCAGAAGAGCAGCTGAGGATCAATGATCAGGCTCAGGAGGATGAGTTGCTTGCTATGGAGTCCATTTTCGGAGACAATGTTTTCATCCTTGAAAGACAACGAGGCCTTCGATGTTTTCAGATTCATATACACATTGAAGCTCCTGGTGAAATTGCTGTTTCTACTAAGTTGAATTCATCTAGTGACCTTAAGTCCACAAGTAGTAGTTCTGATGACTTCTCATACTCCTTCACAGTCCACCATCTTCCACCAATTATGTTGACATGTGCGTTACCCAAATCATACCCAAGCCACCAACCACCTTATTTCACACTATCTGTTCAATGGTTGGATTCTATTAAAATTTCCAATTTGTGTTCCATGCTGGACTCATTATGGATGGACCAACAAGGACAGGAGGTTATATACCAATGGGTGGAATGGCTACAAAGTTCTTCGCTTTCTCATCTTGGGTTTAATAAGGAAATAATGCTTGGTCCTTATGGTATTAGAGATGCTGGAGATAGACGTGCACTCACAGGAAGTGTATCCCTGGATGCAGATATTCCTTTTCTTCGAAGTTACAATAATGATAGACTCCATGAGAACTTCCGCAATAGTTTGCAGGAATGCTGCATCTGTTTTACTGAGTATGCAGGCGCTGAGTTTGTCAGGCTGCCTTGCAAGCATTTCTTCTGTTGGAAATGCATGAAGACTTATTCTGACATCCATGTCAAGGAAGGTACCATCACCAAATTGAACTGTCCTGATGCAAAATGTCAGGGTATGATCCCGCCAGGTTTGCTAAAACGATTGCTTGGTGATGAAGAGTTTGAGCGTTGGGAGTCCTTGATGTTAGAAAAAACACTTGAGTCAATGTCTGATATTGTGTCTTGTCCAAGATGTAAAACACCCTGCATAGAAGATGAAGATGATCATGCCCAATGCTCAAAGTGCTTCTTTAGCTTCTGTACACTTTGCTGGGAGCGGCGCCATGTGGGTGTTCAATGTGTGACACCAGAAATGAAGCTCCGTATCCTCCAGGAACGTCAAAACTTCAGTCAGTTAGGTGAGGAGCAAAAGCGGAGAGAAAAAGAAATGATCAATGAGATCCTCAGTGTGACAGAAATACTTAGCAATGCAAAGCAATGCCCGGCTTGCAACATGGCAATATCCCGAGTTGAAGGTTGCAACAAGATGGTGTGTGAAAATTGTGGGCAGTACTTCTGCTACAACTGTGGAAGCGGAATAACTGGATATGAGCATTTCAGGGATGGGCAATGTGAACTGTTCCCCCAAGAAACAATTCAGAACTGGCAGGCACAGATGAATCCTCGCCAGAATTTGGGTCAGATACATGCTCAACAGGTCGCTGAACTGGTTGCCCAGCAGGTAGCTGTACACGGACAATTATGTCCCAGTTGTGGTCAGTTTAATGCAAAGTTTAACAATAACAATCATATATCTTGCTGGGCATGCCAACACCACTTTTGCTACTTATGCAAAGAGACCGTGAGGCGAAGCTCTCAGCATTATGGACCGAAGGGTTGCAAACAACACACACCAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0023051 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033143 GO:0035257 GO:0035258 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044444 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051427 GO:0051716 GO:0060255 GO:0060765 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1990234 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RWD zf-C3HC4

Protein Analysis

689

Amino Acids

77.77

Weight (kDa)

5.87

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 183 - 317 3.8e-23 RWD domain
zf-RING_2 PF13639 375 - 411 7.7e-06 Ring finger domain
IBR PF01485 445 - 503 1.1e-08 IBR domain, a half RING-finger domain
IBR PF01485 544 - 581 1.4e-06 IBR domain, a half RING-finger domain
IBR_1 PF22191 546 - 580 1.2e-06 IBR domain
Rcat_RNF216 PF26200 547 - 591 1.3e-08 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1229, 1911
Acc36I ACCTGC 1 cut(s) 1879
Acc65I GGTACC 1 cut(s) 1241
AccB1I GGYRCC 3 cut(s) 227, 1241, 1488
AccB7I CCANNNNNTGG 2 cut(s) 934, 1843
AccBSI CCGCTC 1 cut(s) 1486
AccII CGCG 3 cut(s) 63, 389, 498
AciI CCGC 8 cut(s) 26, 63, 498, 1111, 1292, 1486, 1582, 1743
AclWI GGATC 4 cut(s) 40, 545, 1282, 1603
AcsI RAATTY 3 cut(s) 691, 861, 1840
AcuI CTGAAG 2 cut(s) 116, 1540
AcyI GRCGYC 1 cut(s) 1489
AfaI GTAC 4 cut(s) 1243, 1471, 1721, 1899
AfiI CCNNNNNNNGG 4 cut(s) 934, 1234, 1843, 1922
AflII CTTAAG 1 cut(s) 707
AflIII ACRYGT 1 cut(s) 782
AgsI TTSAA 9 cut(s) 241, 334, 611, 656, 691, 842, 1258, 1505, 1682
AhdI GACNNNNNGTC 1 cut(s) 709
AjiI CACGTC 1 cut(s) 1028
AjnI CCWGG 6 cut(s) 215, 661, 1050, 1294, 1539, 2062
AjuI GAANNNNNNNTTGG 8 cut(s) 251, 283, 393, 425, 975, 1007, 1788, 1820
AluBI AGCT 7 cut(s) 490, 532, 659, 1464, 1528, 1895, 2022
AluI AGCT 7 cut(s) 490, 532, 659, 1464, 1528, 1895, 2022
Alw21I GWGCWC 1 cut(s) 1033
Alw26I GTCTC 3 cut(s) 354, 585, 2000
Alw44I GTGCAC 1 cut(s) 1029
AlwI GGATC 4 cut(s) 40, 545, 1282, 1603
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 1674
AoxI GGCC 1 cut(s) 622
ApaLI GTGCAC 1 cut(s) 1029
ApeKI GCWGC 6 cut(s) 431, 490, 493, 529, 1131, 1174
ApoI RAATTY 3 cut(s) 691, 861, 1840
ArsI GACNNNNNNTTYG 2 cut(s) 1543, 1575
Asp700I GAANNNNTTC 1 cut(s) 1803
Asp718I GGTACC 1 cut(s) 1241
AspLEI GCGC 4 cut(s) 389, 498, 1160, 1491
AspS9I GGNCC 3 cut(s) 901, 995, 2036
AsuC2I CCSGG 1 cut(s) 1652
AsuHPI GGTGA 7 cut(s) 52, 129, 377, 677, 1240, 1331, 1580
AsuII TTCGAA 3 cut(s) 281, 311, 1075
AvaI CYCGRG 1 cut(s) 1674
AvaII GGWCC 3 cut(s) 901, 995, 2036
BaeGI GKGCMC 2 cut(s) 232, 1033
BanI GGYRCC 3 cut(s) 227, 1241, 1488
BbsI GAAGAC 1 cut(s) 1217
Bbv12I GWGCWC 1 cut(s) 1033
BbvCI CCTCAGC 1 cut(s) 533
BbvI GCAGC 6 cut(s) 418, 477, 505, 541, 1118, 1161
BccI CCATC 5 cut(s) 768, 892, 1253, 1690, 1766
BciT130I CCWGG 6 cut(s) 217, 663, 1052, 1296, 1541, 2064
BciVI GTATCC 2 cut(s) 1057, 1544
BclI TGATCA 3 cut(s) 544, 1432, 1599
BcnI CCSGG 1 cut(s) 1652
BcoDI GTCTC 3 cut(s) 354, 585, 2000
BfaI CTAG 1 cut(s) 699
BfmI CTRYAG 1 cut(s) 491
BfoI RGCGCY 2 cut(s) 1161, 1492
BfrI CTTAAG 1 cut(s) 707
BfuAI ACCTGC 1 cut(s) 1879
BfuI GTATCC 2 cut(s) 1057, 1544
BisI GCNGC 7 cut(s) 432, 491, 494, 530, 1132, 1175, 1487
BlsI GCNGC 7 cut(s) 433, 492, 495, 531, 1133, 1176, 1488
BmcAI AGTACT 1 cut(s) 1721
Bme1390I CCNGG 7 cut(s) 217, 663, 1052, 1296, 1541, 1652, 2064
Bme18I GGWCC 3 cut(s) 901, 995, 2036
BmeRI GACNNNNNGTC 1 cut(s) 709
BmeT110I CYCGRG 1 cut(s) 1674
BmgBI CACGTC 1 cut(s) 1028
BmgT120I GGNCC 3 cut(s) 901, 995, 2036
BmiI GGNNCC 3 cut(s) 229, 1243, 1490
BmrFI CCNGG 7 cut(s) 217, 663, 1052, 1296, 1541, 1652, 2064
BmrI ACTGGG 1 cut(s) 1911
BmsI GCATC 7 cut(s) 100, 221, 457, 1003, 1045, 1143, 1258
BmuI ACTGGG 1 cut(s) 1911
BpiI GAAGAC 1 cut(s) 1217
BpmI CTGGAG 2 cut(s) 1038, 1523
Bpu10I CCTNAGC 3 cut(s) 435, 533, 552
Bpu14I TTCGAA 3 cut(s) 281, 311, 1075
BpuEI CTTGAG 1 cut(s) 1388
BpuMI CCSGG 1 cut(s) 1652
BsaHI GRCGYC 1 cut(s) 1489
BsaI GGTCTC 2 cut(s) 354, 2000
BsaJI CCNNGG 3 cut(s) 203, 245, 1050
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 4 cut(s) 548, 578, 582, 612
Bsc4I CCNNNNNNNGG 4 cut(s) 934, 1234, 1843, 1922
Bse1I ACTGG 4 cut(s) 1756, 1817, 1881, 1917
Bse3DI GCAATG 3 cut(s) 1642, 1652, 1784
BseBI CCWGG 6 cut(s) 217, 663, 1052, 1296, 1541, 2064
BseDI CCNNGG 3 cut(s) 203, 245, 1050
BseGI GGATG 7 cut(s) 212, 565, 603, 903, 1060, 1224, 1777
BseLI CCNNNNNNNGG 4 cut(s) 934, 1234, 1843, 1922
BseMI GCAATG 3 cut(s) 1642, 1652, 1784
BseNI ACTGG 4 cut(s) 1756, 1817, 1881, 1917
BseRI GAGGAG 3 cut(s) 81, 84, 1586
BseSI GKGCMC 2 cut(s) 232, 1033
BseXI GCAGC 6 cut(s) 418, 477, 505, 541, 1118, 1161
BseYI CCCAGC 3 cut(s) 1479, 1884, 1968
Bsh1236I CGCG 3 cut(s) 63, 389, 498
BshFI GGCC 1 cut(s) 624
BshNI GGYRCC 3 cut(s) 227, 1241, 1488
BsiHKAI GWGCWC 1 cut(s) 1033
BsiHKCI CYCGRG 1 cut(s) 1674
BsiSI CCGG 3 cut(s) 38, 512, 1652
BslFI GGGAC 1 cut(s) 1899
BslI CCNNNNNNNGG 4 cut(s) 934, 1234, 1843, 1922
BsmAI GTCTC 3 cut(s) 354, 585, 2000
BsmFI GGGAC 1 cut(s) 1899
BsmI GAATGC 2 cut(s) 433, 1133
BsnI GGCC 1 cut(s) 624
Bso31I GGTCTC 2 cut(s) 354, 2000
BsoBI CYCGRG 1 cut(s) 1674
Bsp119I TTCGAA 3 cut(s) 281, 311, 1075
Bsp1286I GDGCHC 2 cut(s) 232, 1033
Bsp1407I TGTACA 2 cut(s) 1469, 1897
Bsp143I GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
BspACI CCGC 8 cut(s) 26, 63, 498, 1111, 1292, 1486, 1582, 1743
BspANI GGCC 1 cut(s) 624
BspFNI CGCG 3 cut(s) 63, 389, 498
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 3 cut(s) 229, 1243, 1490
BspMAI CTGCAG 1 cut(s) 495
BspMI ACCTGC 1 cut(s) 1879
BspPI GGATC 4 cut(s) 40, 545, 1282, 1603
BspQI GCTCTTC 3 cut(s) 480, 501, 519
BspT104I TTCGAA 3 cut(s) 281, 311, 1075
BspT107I GGYRCC 3 cut(s) 227, 1241, 1488
BspTI CTTAAG 1 cut(s) 707
BspTNI GGTCTC 2 cut(s) 354, 2000
BsrBI CCGCTC 1 cut(s) 1486
BsrDI GCAATG 3 cut(s) 1642, 1652, 1784
BsrGI TGTACA 2 cut(s) 1469, 1897
BsrI ACTGG 4 cut(s) 1756, 1817, 1881, 1917
BssECI CCNNGG 3 cut(s) 203, 245, 1050
BssMI GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
BssNI GRCGYC 1 cut(s) 1489
Bst2UI CCWGG 6 cut(s) 217, 663, 1052, 1296, 1541, 2064
Bst4CI ACNGT 5 cut(s) 754, 1262, 1736, 1788, 2011
Bst6I CTCTTC 6 cut(s) 49, 396, 480, 501, 519, 1320
BstACI GRCGYC 1 cut(s) 1489
BstAFI CTTAAG 1 cut(s) 707
BstAUI TGTACA 2 cut(s) 1469, 1897
BstBI TTCGAA 3 cut(s) 281, 311, 1075
BstC8I GCNNGC 6 cut(s) 570, 1156, 1184, 1657, 1818, 1975
BstF5I GGATG 7 cut(s) 212, 565, 603, 903, 1060, 1224, 1777
BstFNI CGCG 3 cut(s) 63, 389, 498
BstH2I RGCGCY 2 cut(s) 1161, 1492
BstHHI GCGC 4 cut(s) 389, 498, 1160, 1491
BstKTI GATC 8 cut(s) 35, 130, 540, 547, 1290, 1435, 1602, 1611
BstMAI GTCTC 3 cut(s) 354, 585, 2000
BstMBI GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
BstMWI GCNNNNNNNGC 7 cut(s) 1183, 1300, 1452, 1461, 1656, 1974, 1998
BstNI CCWGG 6 cut(s) 217, 663, 1052, 1296, 1541, 2064
BstNSI RCATGY 3 cut(s) 786, 1859, 1977
BstSCI CCNGG 7 cut(s) 215, 661, 1050, 1294, 1539, 1650, 2062
BstSFI CTRYAG 1 cut(s) 491
BstSLI GKGCMC 2 cut(s) 232, 1033
BstUI CGCG 3 cut(s) 63, 389, 498
BstV1I GCAGC 6 cut(s) 418, 477, 505, 541, 1118, 1161
BstV2I GAAGAC 1 cut(s) 1217
BstX2I RGATCY 2 cut(s) 32, 1608
BstYI RGATCY 2 cut(s) 32, 1608
BsuI GTATCC 2 cut(s) 1057, 1544
BsuRI GGCC 1 cut(s) 624
BtrI CACGTC 1 cut(s) 1028
BtsCI GGATG 7 cut(s) 212, 565, 603, 903, 1060, 1224, 1777
BtsIMutI CAGTG 1 cut(s) 1621
BveI ACCTGC 1 cut(s) 1879
Cac8I GCNNGC 6 cut(s) 570, 1156, 1184, 1657, 1818, 1975
CaiI CAGNNNCTG 1 cut(s) 1160
CciI TCATGA 1 cut(s) 163
CfoI GCGC 4 cut(s) 389, 498, 1160, 1491
Cfr13I GGNCC 3 cut(s) 901, 995, 2036
CseI GACGC 1 cut(s) 69
Csp6I GTAC 4 cut(s) 1242, 1470, 1720, 1898
CviQI GTAC 4 cut(s) 1242, 1470, 1720, 1898
DinI GGCGCC 1 cut(s) 1490
DpnI GATC 8 cut(s) 34, 129, 539, 546, 1289, 1434, 1601, 1610
DpnII GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
DrdI GACNNNNNNGTC 2 cut(s) 1229, 1911
DriI GACNNNNNGTC 1 cut(s) 709
DseDI GACNNNNNNGTC 2 cut(s) 1229, 1911
Eam1104I CTCTTC 6 cut(s) 49, 396, 480, 501, 519, 1320
Eam1105I GACNNNNNGTC 1 cut(s) 709
EarI CTCTTC 6 cut(s) 49, 396, 480, 501, 519, 1320
Eco147I AGGCCT 1 cut(s) 624
Eco31I GGTCTC 2 cut(s) 354, 2000
Eco47I GGWCC 3 cut(s) 901, 995, 2036
Eco57I CTGAAG 2 cut(s) 116, 1540
Eco88I CYCGRG 1 cut(s) 1674
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 6 cut(s) 215, 661, 1050, 1294, 1539, 2062
EcoT22I ATGCAT 2 cut(s) 115, 1208
EgeI GGCGCC 1 cut(s) 1490
EheI GGCGCC 1 cut(s) 1490
FalI AAGNNNNNCTT 4 cut(s) 1175, 1207, 1442, 1474
FaqI GGGAC 1 cut(s) 1899
FauI CCCGC 1 cut(s) 1299
FbaI TGATCA 3 cut(s) 544, 1432, 1599
Fnu4HI GCNGC 7 cut(s) 432, 491, 494, 530, 1132, 1175, 1487
FokI GGATG 7 cut(s) 199, 572, 590, 910, 1067, 1211, 1784
Fsp4HI GCNGC 7 cut(s) 432, 491, 494, 530, 1132, 1175, 1487
FspBI CTAG 1 cut(s) 699
GlaI GCGC 4 cut(s) 388, 497, 1159, 1490
GluI GCNGC 7 cut(s) 432, 491, 494, 530, 1132, 1175, 1487
GsaI CCCAGC 3 cut(s) 1483, 1888, 1972
GsuI CTGGAG 2 cut(s) 1038, 1523
HaeII RGCGCY 2 cut(s) 1161, 1492
HaeIII GGCC 1 cut(s) 624
HapII CCGG 3 cut(s) 38, 512, 1652
HgaI GACGC 1 cut(s) 69
HhaI GCGC 4 cut(s) 389, 498, 1160, 1491
Hin1I GRCGYC 1 cut(s) 1489
Hin6I GCGC 4 cut(s) 387, 496, 1158, 1489
HinP1I GCGC 4 cut(s) 387, 496, 1158, 1489
HincII GTYRAC 1 cut(s) 780
HindII GTYRAC 1 cut(s) 780
HpaII CCGG 3 cut(s) 38, 512, 1652
HphI GGTGA 7 cut(s) 52, 129, 377, 677, 1240, 1331, 1580
Hpy166II GTNNAC 7 cut(s) 714, 757, 780, 1031, 1472, 1784, 1900
Hpy188III TCNNGA 5 cut(s) 164, 256, 554, 1265, 1674
Hpy8I GTNNAC 7 cut(s) 714, 757, 780, 1031, 1472, 1784, 1900
Hpy99I CGWCG 2 cut(s) 11, 110
HpyAV CCTTC 6 cut(s) 102, 635, 757, 1232, 1676, 2035
HpyCH4III ACNGT 5 cut(s) 754, 1262, 1736, 1788, 2011
HpyCH4IV ACGT 2 cut(s) 1027, 1546
HpyF10VI GCNNNNNNNGC 7 cut(s) 1183, 1300, 1452, 1461, 1656, 1974, 1998
HpySE526I ACGT 2 cut(s) 1027, 1546
Hsp92I GRCGYC 1 cut(s) 1489
HspAI GCGC 4 cut(s) 387, 496, 1158, 1489
KasI GGCGCC 1 cut(s) 1488
KpnI GGTACC 1 cut(s) 1245
Ksp22I TGATCA 3 cut(s) 544, 1432, 1599
Kzo9I GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
LguI GCTCTTC 3 cut(s) 480, 501, 519
LmnI GCTCC 4 cut(s) 664, 1483, 1533, 1573
Lsp1109I GCAGC 6 cut(s) 418, 477, 505, 541, 1118, 1161
LweI GCATC 7 cut(s) 100, 221, 457, 1003, 1045, 1143, 1258
MaeI CTAG 1 cut(s) 699
MaeII ACGT 2 cut(s) 1027, 1546
MaeIII GTNAC 7 cut(s) 135, 472, 701, 789, 1079, 1510, 1618
MalI GATC 8 cut(s) 34, 129, 539, 546, 1289, 1434, 1601, 1610
MbiI CCGCTC 1 cut(s) 1486
MboI GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
MflI RGATCY 2 cut(s) 32, 1608
MhlI GDGCHC 2 cut(s) 232, 1033
Mly113I GGCGCC 1 cut(s) 1489
MlyI GAGTC 6 cut(s) 154, 587, 881, 1089, 1352, 1379
MmeI TCCRAC 2 cut(s) 828, 1178
Mph1103I ATGCAT 2 cut(s) 115, 1208
MroXI GAANNNNTTC 1 cut(s) 1803
MseI TTAA 6 cut(s) 116, 708, 858, 975, 1932, 1944
MslI CAYNNNNRTG 1 cut(s) 1497
MspA1I CMGCKG 1 cut(s) 532
MspCI CTTAAG 1 cut(s) 707
MspI CCGG 3 cut(s) 38, 512, 1652
MspR9I CCNGG 7 cut(s) 217, 663, 1052, 1296, 1541, 1652, 2064
Mva1269I GAATGC 2 cut(s) 433, 1133
MvaI CCWGG 6 cut(s) 217, 663, 1052, 1296, 1541, 2064
MvnI CGCG 3 cut(s) 63, 389, 498
MwoI GCNNNNNNNGC 7 cut(s) 1183, 1300, 1452, 1461, 1656, 1974, 1998
NarI GGCGCC 1 cut(s) 1489
NciI CCSGG 1 cut(s) 1652
NdeII GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
NlaIV GGNNCC 3 cut(s) 229, 1243, 1490
NmuCI GTSAC 5 cut(s) 135, 472, 701, 1510, 1618
NsiI ATGCAT 2 cut(s) 115, 1208
NspI RCATGY 3 cut(s) 786, 1859, 1977
NspV TTCGAA 3 cut(s) 281, 311, 1075
PaeI GCATGC 1 cut(s) 1977
PagI TCATGA 1 cut(s) 163
PceI AGGCCT 1 cut(s) 624
PciI ACATGT 1 cut(s) 782
PciSI GCTCTTC 3 cut(s) 480, 501, 519
PcsI WCGNNNNNNNCGW 1 cut(s) 625
PctI GAATGC 2 cut(s) 433, 1133
PdmI GAANNNNTTC 1 cut(s) 1803
PfeI GAWTC 4 cut(s) 220, 640, 851, 1828
PflMI CCANNNNNTGG 2 cut(s) 934, 1843
PfoI TCCNGGA 2 cut(s) 215, 1539
PkrI GCNGC 7 cut(s) 433, 492, 495, 531, 1133, 1176, 1488
PleI GAGTC 6 cut(s) 154, 586, 881, 1089, 1351, 1378
PluTI GGCGCC 1 cut(s) 1492
PpsI GAGTC 6 cut(s) 154, 586, 881, 1089, 1351, 1378
PscI ACATGT 1 cut(s) 782
Psp6I CCWGG 6 cut(s) 215, 661, 1050, 1294, 1539, 2062
PspFI CCCAGC 3 cut(s) 1479, 1884, 1968
PspGI CCWGG 6 cut(s) 215, 661, 1050, 1294, 1539, 2062
PspN4I GGNNCC 3 cut(s) 229, 1243, 1490
PspPI GGNCC 3 cut(s) 901, 995, 2036
PstI CTGCAG 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 2 cut(s) 32, 1608
PvuII CAGCTG 1 cut(s) 532
RsaI GTAC 4 cut(s) 1243, 1471, 1721, 1899
RsaNI GTAC 4 cut(s) 1242, 1470, 1720, 1898
RseI CAYNNNNRTG 1 cut(s) 1497
SapI GCTCTTC 3 cut(s) 480, 501, 519
SaqAI TTAA 6 cut(s) 116, 708, 858, 975, 1932, 1944
SatI GCNGC 7 cut(s) 432, 491, 494, 530, 1132, 1175, 1487
Sau3AI GATC 8 cut(s) 32, 127, 537, 544, 1287, 1432, 1599, 1608
Sau96I GGNCC 3 cut(s) 901, 995, 2036
ScaI AGTACT 1 cut(s) 1721
SchI GAGTC 6 cut(s) 154, 587, 881, 1089, 1352, 1379
ScrFI CCNGG 7 cut(s) 217, 663, 1052, 1296, 1541, 1652, 2064
SduI GDGCHC 2 cut(s) 232, 1033
SfaNI GCATC 7 cut(s) 100, 221, 457, 1003, 1045, 1143, 1258
SfcI CTRYAG 1 cut(s) 491
SfoI GGCGCC 1 cut(s) 1490
SfuI TTCGAA 3 cut(s) 281, 311, 1075
SinI GGWCC 3 cut(s) 901, 995, 2036
SmiMI CAYNNNNRTG 1 cut(s) 1497
SmlI CTYRAG 2 cut(s) 707, 1367
SmoI CTYRAG 2 cut(s) 707, 1367
SphI GCATGC 1 cut(s) 1977
SseBI AGGCCT 1 cut(s) 624
SsiI CCGC 8 cut(s) 26, 63, 498, 1111, 1292, 1486, 1582, 1743
SspDI GGCGCC 1 cut(s) 1488
SspMI CTAG 1 cut(s) 699
StuI AGGCCT 1 cut(s) 624
StyD4I CCNGG 7 cut(s) 215, 661, 1050, 1294, 1539, 1650, 2062
TaaI ACNGT 5 cut(s) 754, 1262, 1736, 1788, 2011
TaiI ACGT 2 cut(s) 1030, 1549
TaqI TCGA 7 cut(s) 105, 223, 281, 311, 504, 628, 1075
TaqII GACCGA 1 cut(s) 2053
TatI WGTACW 3 cut(s) 1469, 1719, 1897
TauI GCSGC 1 cut(s) 1489
TfiI GAWTC 4 cut(s) 220, 640, 851, 1828
Tru1I TTAA 6 cut(s) 116, 708, 858, 975, 1932, 1944
Tru9I TTAA 6 cut(s) 116, 708, 858, 975, 1932, 1944
TscAI CASTG 1 cut(s) 1621
TseFI GTSAC 5 cut(s) 135, 472, 701, 1510, 1618
TseI GCWGC 6 cut(s) 431, 490, 493, 529, 1131, 1174
Tsp45I GTSAC 5 cut(s) 135, 472, 701, 1510, 1618
TspGWI ACGGA 4 cut(s) 24, 45, 1520, 1917
TspRI CASTG 1 cut(s) 1621
Van91I CCANNNNNTGG 2 cut(s) 934, 1843
Vha464I CTTAAG 1 cut(s) 707
VneI GTGCAC 1 cut(s) 1029
VpaK11BI GGWCC 3 cut(s) 901, 995, 2036
XapI RAATTY 3 cut(s) 691, 861, 1840
XceI RCATGY 3 cut(s) 786, 1859, 1977
XmnI GAANNNNTTC 1 cut(s) 1803
XspI CTAG 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 1721
Zsp2I ATGCAT 2 cut(s) 115, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.