RchiOBHm_Chr7g0210351

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008811
Physical Location & Seq
Forward (+)
28016796 .. 28023153
6358 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18822

Sequence Viewer

Length: 2109 bp
ATGAACGACAGAGGTAATAACCAAGCGGACGGATCTCCGGTGAGAAAGACCAGAAGAGGACGCAGAGGAGGAGGAGGAGGAGGAGGACGGGAACAACACCACCCTTCTCTCCAAATCGACGATGCATTAAAACTCAACGATCTTCAGTCACCTTTGAGTTGTCAGCCTCAGACGCATGAACCCACCACCTCGACACCCAGAAACCACAGAAATCCGAGGTCGGCATCCGGGAATCGAGGTGCCCATGTATTCAAACCTCGGTTTGTCAAGAAATCTGAGTTGGGTTCTTCTTCGACTGGCGGAGTGGGTTCTTCTTCGAATGGAGTAGTAGTGGGTTCTTCTTCTTCGAAGAATGAACAAGTGGGTGCTTTGAATGATGAGGTGGGGTCTCTGAGTATTGGTGAGGGAGAAAGTGGGCAGCAAGCGAAGCAAGAAGAAGAGAGAGTTGGGGCTAGTGTAGAGAATGCTGCTGAGGTGTCTGAGCGTGGGAAAGAAGAGGATGATAATGCTGTGACAAGATTGGAAGAGCTGCAGCGCGGTGTCGAAGAGCCGGAGTTGTCAGAAGAGCAGCTGAGGATCAATGATCAGGCTCAGGAGGATGAGTTGCTTGCTATGGAGTCCATTTTTGGAGACAACGTTATCATCCTTGAAAGACAACGAGGCCTTCGATGTTTTCAGATTCGTATACACATTGAAGCTCCTGGTGAAATTGCTGTTTCTACTAAGTTGAATTCATCTAGTGACCTTAAGTACACAAGTAGTAGTTCTGATGACTTCTCATACTCCTTCACAGTCCAGCATCTTCCACCAATTATGTTGACATGTGCGTTACCCAAATCATATCCAAGCCACCAGCCACCTTATTTCACACTATCTGTTCAATGGTTGGATTCTATTAAAATTTCCAATTTGTGTTCCATGCTGGACTCATTATGGATGGACCAACAAGGACAGGAGGTTATATACCAATGGGTGGAATGGCTACAAAGTTCTTCTCTTTCTCATCTTGGGTTTAATGAGGAAATAATGCTTGGTCCTTATGGTAGTAGAGATGCTGGAGATAGACGTGCACTCACAGGAATTGTATCCCCGGATGCAGATATTCCTTTTCTTCGAAGTTACAATAATGATAGACTCCATGAGAACTTCCGCAACAGTTTGCAGGAATGCTGCATCTGTTTTACTGAGTATGCAGGCGCTGAGTTTGTCAGGCTGCCTTGCAAGCATTTCTTCTGCTGGAAATGCATGAAGACTTATTCTGACATGCATGTCAAGGAAGGTACCATCACCAAATTGAACTGTCCTGATACAAAATGTCAGGGTATGATCCCACCAGGTTTGCTAAAACGATTGCTTGGCGATGAAGAGTTTGAACGTTGGGAGTCCTTGATGTTAGAAAAAACACTCGATTCAATGTCTGATGTTGTGTCTTGTCCAAGATGTAAAACACCCTGCATAGAAGATGAAGATGATCATGCCCAATGCTCAAAGTGCTTCTTTAGCTTCTGTACACTGTGCTGGGAGCGGCGTCATGTGGGTGTTCAATGTATGACACCAGAAATGAAGCTCCGTATCCTCCAGGAACGTCAAAACTTCAGTCAGTTAGGCGATGAGCAAAAGCGGAGAGAAAAAGAAATGATCAATGAGATCCTCAGTGTGAGAGTAATACTTAGCGATGCAAAGCAATGCCCGGCTTGCAACATGGCAATATCGCGAGTTGAAGGTTGCAACAAGATGGTGTGTGAAAATTGTGGGCAGTACTTCTGCTACAACTGTGGAAGCGGAATAACGGGATATGAACATTTCAGGGATGGGCAATGTGAACTGTTCCCCCAAGAAACAATTCGGAACTGGCAGGCACAGATGAATCCTCGCCAGAATTTGGGTCAGATACACGCTGAACAGGTCGCTGAACTGGTTGCCCAACAGGTAGCTGTACACGGACAATTATGTCCCAGTTGTGGTCAGTTTAATGCAAAGTTTAACAACAACAATCATATATCTTGCTGGGCATGCCAACACCACTTTTGCTACTTATGCAAAGAGACCGTGAGGCGAAGCTCTCAGCATTATGGACCGAAGGGTTGCAAACAACACACACCAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0023051 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033143 GO:0035257 GO:0035258 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044444 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051427 GO:0051716 GO:0060255 GO:0060765 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1990234 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RWD zf-C3HC4

Protein Analysis

702

Amino Acids

78.88

Weight (kDa)

5.76

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 196 - 330 3.5e-24 RWD domain
zf-RING_2 PF13639 388 - 425 6e-06 Ring finger domain
IBR PF01485 458 - 516 1.8e-08 IBR domain, a half RING-finger domain
IBR PF01485 559 - 594 3.6e-06 IBR domain, a half RING-finger domain
IBR_1 PF22191 560 - 593 2.2e-06 IBR domain
Rcat_RNF216 PF26200 561 - 604 1.8e-08 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1268, 1950
Acc65I GGTACC 1 cut(s) 1280
AccB1I GGYRCC 2 cut(s) 239, 1280
AccB7I CCANNNNNTGG 2 cut(s) 973, 1882
AccBSI CCGCTC 1 cut(s) 1525
AccI GTMKAC 1 cut(s) 685
AccII CGCG 2 cut(s) 537, 1714
AciI CCGC 7 cut(s) 26, 300, 537, 1150, 1525, 1621, 1782
AclI AACGTT 2 cut(s) 636, 1375
AclWI GGATC 4 cut(s) 40, 584, 1321, 1642
AcsI RAATTY 3 cut(s) 730, 900, 1879
AcuI CTGAAG 2 cut(s) 128, 1579
AcyI GRCGYC 1 cut(s) 1528
AfaI GTAC 5 cut(s) 752, 1282, 1510, 1760, 1938
AfiI CCNNNNNNNGG 3 cut(s) 973, 1882, 1961
AflII CTTAAG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 821
AjiI CACGTC 1 cut(s) 1067
AjnI CCWGG 4 cut(s) 700, 1333, 1578, 2101
AjuI GAANNNNNNNTTGG 8 cut(s) 263, 295, 429, 461, 1014, 1046, 1827, 1859
AluBI AGCT 7 cut(s) 529, 571, 698, 1503, 1567, 1934, 2061
AluI AGCT 7 cut(s) 529, 571, 698, 1503, 1567, 1934, 2061
Alw21I GWGCWC 1 cut(s) 1072
Alw26I GTCTC 3 cut(s) 393, 624, 2039
Alw44I GTGCAC 1 cut(s) 1068
AlwI GGATC 4 cut(s) 40, 584, 1321, 1642
AlwNI CAGNNNCTG 1 cut(s) 1199
AoxI GGCC 1 cut(s) 661
ApaLI GTGCAC 1 cut(s) 1068
ApeKI GCWGC 7 cut(s) 418, 467, 529, 532, 568, 1170, 1213
ApoI RAATTY 3 cut(s) 730, 900, 1879
ArsI GACNNNNNNTTYG 2 cut(s) 1582, 1614
Asp700I GAANNNNTTC 1 cut(s) 1842
Asp718I GGTACC 1 cut(s) 1280
AspLEI GCGC 2 cut(s) 537, 1199
AspS9I GGNCC 3 cut(s) 940, 1034, 2075
AsuC2I CCSGG 3 cut(s) 229, 1091, 1691
AsuHPI GGTGA 5 cut(s) 52, 141, 413, 716, 1279
AsuII TTCGAA 3 cut(s) 317, 347, 1114
AvaII GGWCC 3 cut(s) 940, 1034, 2075
BaeGI GKGCMC 2 cut(s) 244, 1072
BanI GGYRCC 2 cut(s) 239, 1280
BbsI GAAGAC 1 cut(s) 1256
Bbv12I GWGCWC 1 cut(s) 1072
BbvCI CCTCAGC 2 cut(s) 471, 572
BbvI GCAGC 7 cut(s) 430, 454, 516, 544, 580, 1157, 1200
BccI CCATC 4 cut(s) 931, 1292, 1729, 1805
BciT130I CCWGG 4 cut(s) 702, 1335, 1580, 2103
BciVI GTATCC 2 cut(s) 1096, 1583
BclI TGATCA 3 cut(s) 583, 1471, 1638
BcnI CCSGG 3 cut(s) 229, 1091, 1691
BcoDI GTCTC 3 cut(s) 393, 624, 2039
BfaI CTAG 2 cut(s) 453, 738
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 1200
BfrI CTTAAG 1 cut(s) 746
BfuI GTATCC 2 cut(s) 1096, 1583
BisI GCNGC 8 cut(s) 419, 468, 530, 533, 569, 1171, 1214, 1526
BlsI GCNGC 8 cut(s) 420, 469, 531, 534, 570, 1172, 1215, 1527
BmcAI AGTACT 1 cut(s) 1760
Bme1390I CCNGG 7 cut(s) 229, 702, 1091, 1335, 1580, 1691, 2103
Bme18I GGWCC 3 cut(s) 940, 1034, 2075
BmgBI CACGTC 1 cut(s) 1067
BmgT120I GGNCC 3 cut(s) 940, 1034, 2075
BmiI GGNNCC 2 cut(s) 241, 1282
BmrFI CCNGG 7 cut(s) 229, 702, 1091, 1335, 1580, 1691, 2103
BmrI ACTGGG 1 cut(s) 1950
BmsI GCATC 7 cut(s) 112, 233, 808, 1042, 1084, 1182, 1666
BmuI ACTGGG 1 cut(s) 1950
BpiI GAAGAC 1 cut(s) 1256
BpmI CTGGAG 2 cut(s) 1077, 1562
Bpu10I CCTNAGC 3 cut(s) 471, 572, 591
Bpu14I TTCGAA 3 cut(s) 317, 347, 1114
BpuMI CCSGG 3 cut(s) 229, 1091, 1691
BsaHI GRCGYC 1 cut(s) 1528
BsaI GGTCTC 2 cut(s) 393, 2039
BsaJI CCNNGG 3 cut(s) 215, 257, 1089
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 6 cut(s) 315, 345, 587, 617, 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 973, 1882, 1961
Bse1I ACTGG 4 cut(s) 301, 1856, 1920, 1956
Bse3DI GCAATG 2 cut(s) 1691, 1823
BseBI CCWGG 4 cut(s) 702, 1335, 1580, 2103
BseDI CCNNGG 3 cut(s) 215, 257, 1089
BseGI GGATG 7 cut(s) 224, 505, 604, 642, 942, 1099, 1816
BseLI CCNNNNNNNGG 3 cut(s) 973, 1882, 1961
BseMI GCAATG 2 cut(s) 1691, 1823
BseNI ACTGG 4 cut(s) 301, 1856, 1920, 1956
BseRI GAGGAG 6 cut(s) 81, 84, 87, 90, 93, 96
BseSI GKGCMC 2 cut(s) 244, 1072
BseXI GCAGC 7 cut(s) 430, 454, 516, 544, 580, 1157, 1200
BseYI CCCAGC 2 cut(s) 1518, 2007
Bsh1236I CGCG 2 cut(s) 537, 1714
BshFI GGCC 1 cut(s) 663
BshNI GGYRCC 2 cut(s) 239, 1280
BsiHKAI GWGCWC 1 cut(s) 1072
BsiSI CCGG 5 cut(s) 38, 228, 551, 1091, 1691
BslFI GGGAC 1 cut(s) 1938
BslI CCNNNNNNNGG 3 cut(s) 973, 1882, 1961
BsmAI GTCTC 3 cut(s) 393, 624, 2039
BsmFI GGGAC 1 cut(s) 1938
BsmI GAATGC 2 cut(s) 469, 1172
BsnI GGCC 1 cut(s) 663
Bso31I GGTCTC 2 cut(s) 393, 2039
Bsp119I TTCGAA 3 cut(s) 317, 347, 1114
Bsp1286I GDGCHC 2 cut(s) 244, 1072
Bsp1407I TGTACA 2 cut(s) 1508, 1936
Bsp143I GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
Bsp68I TCGCGA 1 cut(s) 1714
BspACI CCGC 7 cut(s) 26, 300, 537, 1150, 1525, 1621, 1782
BspANI GGCC 1 cut(s) 663
BspFNI CGCG 2 cut(s) 537, 1714
BspLI GGNNCC 2 cut(s) 241, 1282
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 4 cut(s) 40, 584, 1321, 1642
BspQI GCTCTTC 3 cut(s) 519, 540, 558
BspT104I TTCGAA 3 cut(s) 317, 347, 1114
BspT107I GGYRCC 2 cut(s) 239, 1280
BspTI CTTAAG 1 cut(s) 746
BspTNI GGTCTC 2 cut(s) 393, 2039
BsrBI CCGCTC 1 cut(s) 1525
BsrDI GCAATG 2 cut(s) 1691, 1823
BsrGI TGTACA 2 cut(s) 1508, 1936
BsrI ACTGG 4 cut(s) 301, 1856, 1920, 1956
BssECI CCNNGG 3 cut(s) 215, 257, 1089
BssMI GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
BssNAI GTATAC 1 cut(s) 686
BssNI GRCGYC 1 cut(s) 1528
Bst1107I GTATAC 1 cut(s) 686
Bst2UI CCWGG 4 cut(s) 702, 1335, 1580, 2103
Bst4CI ACNGT 7 cut(s) 793, 1157, 1301, 1515, 1775, 1827, 2050
Bst6I CTCTTC 7 cut(s) 49, 432, 489, 519, 540, 558, 1359
BstACI GRCGYC 1 cut(s) 1528
BstAFI CTTAAG 1 cut(s) 746
BstAUI TGTACA 2 cut(s) 1508, 1936
BstBI TTCGAA 3 cut(s) 317, 347, 1114
BstC8I GCNNGC 7 cut(s) 423, 609, 1195, 1223, 1696, 1857, 2014
BstF5I GGATG 7 cut(s) 224, 505, 604, 642, 942, 1099, 1816
BstFNI CGCG 2 cut(s) 537, 1714
BstH2I RGCGCY 1 cut(s) 1200
BstHHI GCGC 2 cut(s) 537, 1199
BstKTI GATC 8 cut(s) 35, 142, 579, 586, 1329, 1474, 1641, 1650
BstMAI GTCTC 3 cut(s) 393, 624, 2039
BstMBI GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
BstMWI GCNNNNNNNGC 9 cut(s) 172, 427, 1222, 1242, 1491, 1500, 1695, 2013, 2037
BstNI CCWGG 4 cut(s) 702, 1335, 1580, 2103
BstNSI RCATGY 4 cut(s) 825, 1267, 1271, 2016
BstSCI CCNGG 7 cut(s) 227, 700, 1089, 1333, 1578, 1689, 2101
BstSFI CTRYAG 1 cut(s) 530
BstSLI GKGCMC 2 cut(s) 244, 1072
BstUI CGCG 2 cut(s) 537, 1714
BstV1I GCAGC 7 cut(s) 430, 454, 516, 544, 580, 1157, 1200
BstV2I GAAGAC 1 cut(s) 1256
BstX2I RGATCY 2 cut(s) 32, 1647
BstYI RGATCY 2 cut(s) 32, 1647
BstZ17I GTATAC 1 cut(s) 686
BsuI GTATCC 2 cut(s) 1096, 1583
BsuRI GGCC 1 cut(s) 663
BtgZI GCGATG 3 cut(s) 1374, 1623, 1689
BtrI CACGTC 1 cut(s) 1067
BtsCI GGATG 7 cut(s) 224, 505, 604, 642, 942, 1099, 1816
BtsIMutI CAGTG 2 cut(s) 1511, 1660
BtuMI TCGCGA 1 cut(s) 1714
Cac8I GCNNGC 7 cut(s) 423, 609, 1195, 1223, 1696, 1857, 2014
CaiI CAGNNNCTG 1 cut(s) 1199
CfoI GCGC 2 cut(s) 537, 1199
Cfr13I GGNCC 3 cut(s) 940, 1034, 2075
CseI GACGC 3 cut(s) 69, 181, 1517
CsiI ACCWGGT 1 cut(s) 1333
Csp6I GTAC 5 cut(s) 751, 1281, 1509, 1759, 1937
CspCI CAANNNNNGTGG 2 cut(s) 1320, 1355
CviQI GTAC 5 cut(s) 751, 1281, 1509, 1759, 1937
DpnI GATC 8 cut(s) 34, 141, 578, 585, 1328, 1473, 1640, 1649
DpnII GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
DrdI GACNNNNNNGTC 2 cut(s) 1268, 1950
DseDI GACNNNNNNGTC 2 cut(s) 1268, 1950
Eam1104I CTCTTC 7 cut(s) 49, 432, 489, 519, 540, 558, 1359
EarI CTCTTC 7 cut(s) 49, 432, 489, 519, 540, 558, 1359
EciI GGCGGA 1 cut(s) 315
Eco147I AGGCCT 1 cut(s) 663
Eco31I GGTCTC 2 cut(s) 393, 2039
Eco47I GGWCC 3 cut(s) 940, 1034, 2075
Eco57I CTGAAG 2 cut(s) 128, 1579
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 4 cut(s) 700, 1333, 1578, 2101
EcoT22I ATGCAT 3 cut(s) 127, 1247, 1269
FalI AAGNNNNNCTT 4 cut(s) 1214, 1246, 1481, 1513
FaqI GGGAC 1 cut(s) 1938
FbaI TGATCA 3 cut(s) 583, 1471, 1638
FblI GTMKAC 1 cut(s) 685
Fnu4HI GCNGC 8 cut(s) 419, 468, 530, 533, 569, 1171, 1214, 1526
FokI GGATG 7 cut(s) 211, 512, 611, 629, 949, 1106, 1823
Fsp4HI GCNGC 8 cut(s) 419, 468, 530, 533, 569, 1171, 1214, 1526
FspBI CTAG 2 cut(s) 453, 738
GlaI GCGC 2 cut(s) 536, 1198
GluI GCNGC 8 cut(s) 419, 468, 530, 533, 569, 1171, 1214, 1526
GsaI CCCAGC 2 cut(s) 1522, 2011
GsuI CTGGAG 2 cut(s) 1077, 1562
HaeII RGCGCY 1 cut(s) 1200
HaeIII GGCC 1 cut(s) 663
HapII CCGG 5 cut(s) 38, 228, 551, 1091, 1691
HgaI GACGC 3 cut(s) 69, 181, 1517
HhaI GCGC 2 cut(s) 537, 1199
Hin1I GRCGYC 1 cut(s) 1528
Hin6I GCGC 2 cut(s) 535, 1197
HinP1I GCGC 2 cut(s) 535, 1197
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 9 cut(s) 232, 617, 679, 890, 926, 1134, 1382, 1409, 1867
HpaII CCGG 5 cut(s) 38, 228, 551, 1091, 1691
HphI GGTGA 5 cut(s) 52, 141, 413, 716, 1279
Hpy166II GTNNAC 7 cut(s) 686, 753, 819, 1070, 1511, 1823, 1939
Hpy188III TCNNGA 4 cut(s) 268, 593, 1304, 1713
Hpy8I GTNNAC 7 cut(s) 686, 753, 819, 1070, 1511, 1823, 1939
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 6 cut(s) 114, 674, 796, 1271, 1715, 2074
HpyCH4III ACNGT 7 cut(s) 793, 1157, 1301, 1515, 1775, 1827, 2050
HpyCH4IV ACGT 4 cut(s) 636, 1066, 1375, 1585
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 427, 1222, 1242, 1491, 1500, 1695, 2013, 2037
HpySE526I ACGT 4 cut(s) 636, 1066, 1375, 1585
Hsp92I GRCGYC 1 cut(s) 1528
HspAI GCGC 2 cut(s) 535, 1197
KpnI GGTACC 1 cut(s) 1284
Ksp22I TGATCA 3 cut(s) 583, 1471, 1638
Kzo9I GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
LguI GCTCTTC 3 cut(s) 519, 540, 558
LmnI GCTCC 3 cut(s) 703, 1522, 1572
Lsp1109I GCAGC 7 cut(s) 430, 454, 516, 544, 580, 1157, 1200
LweI GCATC 7 cut(s) 112, 233, 808, 1042, 1084, 1182, 1666
MabI ACCWGGT 1 cut(s) 1333
MaeI CTAG 2 cut(s) 453, 738
MaeII ACGT 4 cut(s) 636, 1066, 1375, 1585
MaeIII GTNAC 5 cut(s) 147, 511, 740, 828, 1118
MalI GATC 8 cut(s) 34, 141, 578, 585, 1328, 1473, 1640, 1649
MbiI CCGCTC 1 cut(s) 1525
MboI GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
MflI RGATCY 2 cut(s) 32, 1647
MhlI GDGCHC 2 cut(s) 244, 1072
MlyI GAGTC 4 cut(s) 626, 920, 1128, 1391
MmeI TCCRAC 1 cut(s) 867
Mph1103I ATGCAT 3 cut(s) 127, 1247, 1269
MroXI GAANNNNTTC 1 cut(s) 1842
MseI TTAA 6 cut(s) 128, 747, 897, 1014, 1971, 1983
MslI CAYNNNNRTG 1 cut(s) 1536
MspA1I CMGCKG 1 cut(s) 571
MspCI CTTAAG 1 cut(s) 746
MspI CCGG 5 cut(s) 38, 228, 551, 1091, 1691
MspR9I CCNGG 7 cut(s) 229, 702, 1091, 1335, 1580, 1691, 2103
Mva1269I GAATGC 2 cut(s) 469, 1172
MvaI CCWGG 4 cut(s) 702, 1335, 1580, 2103
MvnI CGCG 2 cut(s) 537, 1714
MwoI GCNNNNNNNGC 9 cut(s) 172, 427, 1222, 1242, 1491, 1500, 1695, 2013, 2037
NciI CCSGG 3 cut(s) 229, 1091, 1691
NdeII GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
NlaIV GGNNCC 2 cut(s) 241, 1282
NmuCI GTSAC 3 cut(s) 147, 511, 740
NruI TCGCGA 1 cut(s) 1714
NsiI ATGCAT 3 cut(s) 127, 1247, 1269
NspI RCATGY 4 cut(s) 825, 1267, 1271, 2016
NspV TTCGAA 3 cut(s) 317, 347, 1114
PaeI GCATGC 1 cut(s) 2016
PceI AGGCCT 1 cut(s) 663
PciI ACATGT 1 cut(s) 821
PciSI GCTCTTC 3 cut(s) 519, 540, 558
PcsI WCGNNNNNNNCGW 1 cut(s) 664
PctI GAATGC 2 cut(s) 469, 1172
PdmI GAANNNNTTC 1 cut(s) 1842
PfeI GAWTC 5 cut(s) 232, 679, 890, 1409, 1867
PflMI CCANNNNNTGG 2 cut(s) 973, 1882
PfoI TCCNGGA 2 cut(s) 227, 1578
PkrI GCNGC 8 cut(s) 420, 469, 531, 534, 570, 1172, 1215, 1527
PleI GAGTC 4 cut(s) 625, 920, 1128, 1390
PpsI GAGTC 4 cut(s) 625, 920, 1128, 1390
PscI ACATGT 1 cut(s) 821
Psp1406I AACGTT 2 cut(s) 636, 1375
Psp6I CCWGG 4 cut(s) 700, 1333, 1578, 2101
PspFI CCCAGC 2 cut(s) 1518, 2007
PspGI CCWGG 4 cut(s) 700, 1333, 1578, 2101
PspN4I GGNNCC 2 cut(s) 241, 1282
PspPI GGNCC 3 cut(s) 940, 1034, 2075
PstI CTGCAG 1 cut(s) 534
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 32, 1647
PvuII CAGCTG 1 cut(s) 571
RruI TCGCGA 1 cut(s) 1714
RsaI GTAC 5 cut(s) 752, 1282, 1510, 1760, 1938
RsaNI GTAC 5 cut(s) 751, 1281, 1509, 1759, 1937
RseI CAYNNNNRTG 1 cut(s) 1536
SapI GCTCTTC 3 cut(s) 519, 540, 558
SaqAI TTAA 6 cut(s) 128, 747, 897, 1014, 1971, 1983
SatI GCNGC 8 cut(s) 419, 468, 530, 533, 569, 1171, 1214, 1526
Sau3AI GATC 8 cut(s) 32, 139, 576, 583, 1326, 1471, 1638, 1647
Sau96I GGNCC 3 cut(s) 940, 1034, 2075
ScaI AGTACT 1 cut(s) 1760
SchI GAGTC 4 cut(s) 626, 920, 1128, 1391
ScrFI CCNGG 7 cut(s) 229, 702, 1091, 1335, 1580, 1691, 2103
SduI GDGCHC 2 cut(s) 244, 1072
SexAI ACCWGGT 1 cut(s) 1333
SfaNI GCATC 7 cut(s) 112, 233, 808, 1042, 1084, 1182, 1666
SfcI CTRYAG 1 cut(s) 530
SfuI TTCGAA 3 cut(s) 317, 347, 1114
SinI GGWCC 3 cut(s) 940, 1034, 2075
SmiMI CAYNNNNRTG 1 cut(s) 1536
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SphI GCATGC 1 cut(s) 2016
SseBI AGGCCT 1 cut(s) 663
SsiI CCGC 7 cut(s) 26, 300, 537, 1150, 1525, 1621, 1782
SspMI CTAG 2 cut(s) 453, 738
StuI AGGCCT 1 cut(s) 663
StyD4I CCNGG 7 cut(s) 227, 700, 1089, 1333, 1578, 1689, 2101
TaaI ACNGT 7 cut(s) 793, 1157, 1301, 1515, 1775, 1827, 2050
TaiI ACGT 4 cut(s) 639, 1069, 1378, 1588
TaqII GACCGA 1 cut(s) 2092
TatI WGTACW 4 cut(s) 750, 1508, 1758, 1936
TauI GCSGC 1 cut(s) 1528
TfiI GAWTC 5 cut(s) 232, 679, 890, 1409, 1867
Tru1I TTAA 6 cut(s) 128, 747, 897, 1014, 1971, 1983
Tru9I TTAA 6 cut(s) 128, 747, 897, 1014, 1971, 1983
TscAI CASTG 2 cut(s) 1518, 1660
TseFI GTSAC 3 cut(s) 147, 511, 740
TseI GCWGC 7 cut(s) 418, 467, 529, 532, 568, 1170, 1213
Tsp45I GTSAC 3 cut(s) 147, 511, 740
TspGWI ACGGA 3 cut(s) 45, 1559, 1956
TspRI CASTG 2 cut(s) 1518, 1660
Van91I CCANNNNNTGG 2 cut(s) 973, 1882
Vha464I CTTAAG 1 cut(s) 746
VneI GTGCAC 1 cut(s) 1068
VpaK11BI GGWCC 3 cut(s) 940, 1034, 2075
XapI RAATTY 3 cut(s) 730, 900, 1879
XceI RCATGY 4 cut(s) 825, 1267, 1271, 2016
XmiI GTMKAC 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 1842
XspI CTAG 2 cut(s) 453, 738
ZrmI AGTACT 1 cut(s) 1760
Zsp2I ATGCAT 3 cut(s) 127, 1247, 1269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.