FvH4_5g22580

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008811
Physical Location & Seq
Forward (+)
13911208 .. 13916446
5239 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22580.t1

Sequence Viewer

Length: 2019 bp
ATGAAGGGAGGCCATAGCCAACCAGAAGAGTCTCCGGCTCTGCTGAGAAAGACCAGAAGAGGAGGAAAGAAACATCACCACCACCACTACCCTTCTATCCAAATTGAGGAGACACCAACTCACAACAATCTGGATCACGATCTTCAATCACCTTCCACTTCTCAGCTTCAGAATCCTGAACCCACCACCGCCTCCGCACCCAGACACCACAGAAGTCCCAGGAATCGAGCCAAACCTCGCTATGTCAAGAAATCTGAGGTGGGTTCTTCTGAAGTGGGTGGTTCTTCCAAGGTGGAGTCTTTGAATGAGCAAGTGGAGTCTTTGAGTATTGGTGAGACAGAAACTGGGGAGCAATCCAAGAAAGAAGAAGAAGAAGAAGAGAGACTTGTTGTGGGTAGTGAAGAGAATGCTGAGGATGATGCTTTGAGAAGATTGGAAGAGTTACAGGTTGGTGTCCAAGAGCCTGAGTTGTCAGAAGAACAGCTGAGGATCAATGATCAGGCTCAGGAGGATGAGTTGCTTGCTATGGAGTCCATTTTTGGAGACAATGTTTTCATCCTTCAAAGACAACGAGGCCTTCGATCTTTCCAGATTCATATACACATTGAAGCTCCTGATGAAATTGCTGTTTCTACCAAGTTGAATTCATCTACTGACCCTAACAACACAAGTAGTTCTGGTGACTTCTCATACTCTTTCACAGTCCAGCATCTTCCACCAATTATGTTGACATGTTCGTTACCCAAATCATACCCAAGCCACCAACCACCTTATTTTACACTATCTGTTCAATGGTTGGATGATATTAAAATTTCCAGTTTGTGTTCCATGTTGGACTCATTATGGATGGACCAACAAGGACAGGAGGTTATATACCAATGGGTGGAATGGCTACAAAGTTCTTCCCTCTCTCATCTTGGGTTTAATAAGGAAATAGTGCTTGGTCCTTATGGCATTAAAGATGTTGGAGATAGACGTGCACTTACAGGAAGTGTATCACCAGATGCAGATATTCCTTTTCTCCGGAGTTACAATAATGAAAAACTCCATGAGAACTTCCTCAATGGTTTGCAGGAATGCTGCATCTGTTTTAGTGTGTATGCAGGTGCTGAGTTTATCAGGCTGCCTTGCAAGCATTTCTTCTGTTGGAAATGCATGAAGACCTTTTCAGACATGCATGTCAGGGAAGGTACCGTTACCAAATTGCATTGTTCTGATGCAAAATGTAAGGGCATGATCCCGCCATGTTTGCTGAAACAATTGCTTGGCAATGAAGAGTTCGAACGTTGGGAATCCTCAATGTTAGTAAAAACACTCGAGTCAATGTCTGATATTGTGAACTGCCCAAGATGTAAAACACCCTGCATAGAAGATGAAGACGATCATGCCCAATGCTCATACTGCTTCTTTAGCTTTTGTACACTTTGCTGGGAGCGGCGCCATGTGGGTGTTCAATGTATGACACCAGAAATGAAGCTCCATATCCTCCAGGAACGTCAAAAGTACTGTCAGTTAGACGATAAGCAAAAGCGGAGAGAAAAAGAATTGATCAATGAGATCCTCAGTGTGAGAGAAATACTTCGTGATTCAAAGCAATGCCCGGCTTGCAACATGGCTATATCCCGAGTTGATGGTTGCAACAAGATGGTTTGTGAAAATTGTGGGCAGTACTTCTGCTACAACTGTGGAAGCGGAATAACAGGATATGATCATTTCAGGGATGATAAATGTGAACTGTTCCCCCAAGAGGCAATTCTCAACTGGGAGGCAAGGATGAATCCTCGCGAGAATTTGGGTCAGATTCAAGCCCAACGGGTTGCCGAACTGGTTGCCGAACAGGTTGACGTACACGGACAGTTATGTCCTAGTTGTGGTCAGTTTAATGCAAAGTTTAACAATAACAATCATATATCCTGCTGGGCATGCCAACACCACTTTTGCTACTTATGCAAAGAGACCGTAAGACGAAGCTCTCAACATTATGGACCGACGGGCTGCAAACAACACACACAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006464 GO:0006807 GO:0007154 GO:0007165 GO:0008134 GO:0008150 GO:0008152 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0023051 GO:0023052 GO:0030162 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031624 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033143 GO:0035257 GO:0035258 GO:0036211 GO:0042176 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044390 GO:0044424 GO:0044444 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048583 GO:0050681 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051427 GO:0051716 GO:0060255 GO:0060765 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1990234 GO:2000058 GO:2000060 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR RWD zf-C3HC4

Protein Analysis

673

Amino Acids

76.84

Weight (kDa)

5.67

Isoelectric Point (pI)

66.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWD PF05773 167 - 300 1.9e-24 RWD domain
IBR PF01485 428 - 486 2.6e-07 IBR domain, a half RING-finger domain
IBR PF01485 527 - 564 1.7e-06 IBR domain, a half RING-finger domain
IBR_1 PF22191 530 - 563 2.8e-06 IBR domain
Rcat_RNF216 PF26200 531 - 574 4.4e-08 RNF216 Rcat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1094
AasI GACNNNNNNGTC 2 cut(s) 1178, 1860
Acc36I ACCTGC 1 cut(s) 1094
Acc65I GGTACC 1 cut(s) 1190
AccB1I GGYRCC 2 cut(s) 1190, 1437
AccB7I CCANNNNNTGG 1 cut(s) 883
AccBSI CCGCTC 1 cut(s) 1435
AccII CGCG 1 cut(s) 1785
AccIII TCCGGA 1 cut(s) 1023
AciI CCGC 6 cut(s) 189, 195, 1241, 1435, 1531, 1692
AclI AACGTT 1 cut(s) 1285
AclWI GGATC 4 cut(s) 141, 497, 1231, 1552
AcsI RAATTY 3 cut(s) 643, 810, 1789
AcuI CTGAAG 2 cut(s) 152, 291
AcyI GRCGYC 1 cut(s) 1438
AfaI GTAC 5 cut(s) 1192, 1420, 1505, 1670, 1848
AfiI CCNNNNNNNGG 4 cut(s) 106, 883, 1747, 1871
AflIII ACRYGT 1 cut(s) 731
AgsI TTSAA 9 cut(s) 146, 304, 563, 608, 643, 791, 1454, 1590, 1805
AjiI CACGTC 1 cut(s) 977
AjnI CCWGG 2 cut(s) 218, 1488
AjuI GAANNNNNNNTTGG 4 cut(s) 924, 956, 1737, 1769
AluBI AGCT 6 cut(s) 166, 484, 611, 1413, 1477, 1971
AluI AGCT 6 cut(s) 166, 484, 611, 1413, 1477, 1971
Alw21I GWGCWC 1 cut(s) 982
Alw26I GTCTC 6 cut(s) 36, 104, 329, 376, 537, 1949
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 4 cut(s) 141, 497, 1231, 1552
AlwNI CAGNNNCTG 2 cut(s) 344, 1109
Ama87I CYCGRG 2 cut(s) 1316, 1623
Aor13HI TCCGGA 1 cut(s) 1023
AoxI GGCC 2 cut(s) 10, 574
ApaLI GTGCAC 1 cut(s) 978
ApeKI GCWGC 3 cut(s) 1080, 1123, 1995
ApoI RAATTY 3 cut(s) 643, 810, 1789
ArsI GACNNNNNNTTYG 2 cut(s) 1492, 1524
Asp700I GAANNNNTTC 1 cut(s) 1578
Asp718I GGTACC 1 cut(s) 1190
AspLEI GCGC 1 cut(s) 1440
AspS9I GGNCC 3 cut(s) 850, 944, 1985
AsuC2I CCSGG 1 cut(s) 1601
AsuHPI GGTGA 5 cut(s) 68, 141, 344, 692, 990
AsuII TTCGAA 1 cut(s) 1281
AvaI CYCGRG 2 cut(s) 1316, 1623
AvaII GGWCC 3 cut(s) 850, 944, 1985
BaeGI GKGCMC 1 cut(s) 982
BanI GGYRCC 2 cut(s) 1190, 1437
BbsI GAAGAC 2 cut(s) 1166, 1383
Bbv12I GWGCWC 1 cut(s) 982
BbvCI CCTCAGC 2 cut(s) 411, 485
BbvI GCAGC 3 cut(s) 1067, 1110, 1982
BccI CCATC 3 cut(s) 841, 1625, 1639
BcgI CGANNNNNNTGC 2 cut(s) 1811, 1845
BciT130I CCWGG 2 cut(s) 220, 1490
BclI TGATCA 3 cut(s) 496, 1548, 1708
BcnI CCSGG 1 cut(s) 1601
BcoDI GTCTC 6 cut(s) 36, 104, 329, 376, 537, 1949
BfaI CTAG 1 cut(s) 1866
BfoI RGCGCY 1 cut(s) 1441
BfuAI ACCTGC 1 cut(s) 1094
BisI GCNGC 4 cut(s) 1081, 1124, 1436, 1996
BlsI GCNGC 4 cut(s) 1082, 1125, 1437, 1997
BmcAI AGTACT 2 cut(s) 1505, 1670
Bme1390I CCNGG 3 cut(s) 220, 1490, 1601
Bme18I GGWCC 3 cut(s) 850, 944, 1985
BmeT110I CYCGRG 2 cut(s) 1316, 1623
BmgBI CACGTC 1 cut(s) 977
BmgT120I GGNCC 3 cut(s) 850, 944, 1985
BmiI GGNNCC 2 cut(s) 1192, 1439
BmrFI CCNGG 3 cut(s) 220, 1490, 1601
BmrI ACTGGG 2 cut(s) 354, 1771
BmsI GCATC 5 cut(s) 409, 718, 994, 1092, 1207
BmuI ACTGGG 2 cut(s) 354, 1771
BpiI GAAGAC 2 cut(s) 1166, 1383
BpmI CTGGAG 1 cut(s) 1472
Bpu10I CCTNAGC 3 cut(s) 411, 485, 504
Bpu14I TTCGAA 1 cut(s) 1281
BpuMI CCSGG 1 cut(s) 1601
BsaBI GATNNNNATC 1 cut(s) 138
BsaHI GRCGYC 1 cut(s) 1438
BsaI GGTCTC 1 cut(s) 1949
BsaJI CCNNGG 2 cut(s) 218, 288
BsaWI WCCGGW 1 cut(s) 1023
BsaXI ACNNNNNCTCC 4 cut(s) 500, 530, 534, 564
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 883, 1747, 1871
Bse1I ACTGG 4 cut(s) 349, 816, 1766, 1830
Bse3DI GCAATG 2 cut(s) 1276, 1601
Bse8I GATNNNNATC 1 cut(s) 138
BseAI TCCGGA 1 cut(s) 1023
BseBI CCWGG 2 cut(s) 220, 1490
BseDI CCNNGG 2 cut(s) 218, 288
BseGI GGATG 7 cut(s) 421, 517, 555, 805, 852, 1726, 1779
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 4 cut(s) 106, 883, 1747, 1871
BseMI GCAATG 2 cut(s) 1276, 1601
BseMII CTCAG 9 cut(s) 35, 176, 246, 402, 456, 476, 518, 1101, 1576
BseNI ACTGG 4 cut(s) 349, 816, 1766, 1830
BseRI GAGGAG 2 cut(s) 75, 122
BseSI GKGCMC 1 cut(s) 982
BseXI GCAGC 3 cut(s) 1067, 1110, 1982
BseYI CCCAGC 2 cut(s) 1428, 1917
Bsh1236I CGCG 1 cut(s) 1785
BshFI GGCC 2 cut(s) 12, 576
BshNI GGYRCC 2 cut(s) 1190, 1437
BsiHKAI GWGCWC 1 cut(s) 982
BsiHKCI CYCGRG 2 cut(s) 1316, 1623
BsiSI CCGG 3 cut(s) 35, 1024, 1601
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 4 cut(s) 106, 883, 1747, 1871
BsmAI GTCTC 6 cut(s) 36, 104, 329, 376, 537, 1949
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 2 cut(s) 412, 1082
BsnI GGCC 2 cut(s) 12, 576
Bso31I GGTCTC 1 cut(s) 1949
BsoBI CYCGRG 2 cut(s) 1316, 1623
Bsp119I TTCGAA 1 cut(s) 1281
Bsp1286I GDGCHC 1 cut(s) 982
Bsp13I TCCGGA 1 cut(s) 1023
Bsp1407I TGTACA 1 cut(s) 1418
Bsp68I TCGCGA 1 cut(s) 1785
BspACI CCGC 6 cut(s) 189, 195, 1241, 1435, 1531, 1692
BspANI GGCC 2 cut(s) 12, 576
BspCNI CTCAG 9 cut(s) 36, 175, 247, 403, 457, 477, 517, 1102, 1575
BspEI TCCGGA 1 cut(s) 1023
BspFNI CGCG 1 cut(s) 1785
BspLI GGNNCC 2 cut(s) 1192, 1439
BspMI ACCTGC 1 cut(s) 1094
BspPI GGATC 4 cut(s) 141, 497, 1231, 1552
BspT104I TTCGAA 1 cut(s) 1281
BspT107I GGYRCC 2 cut(s) 1190, 1437
BspTNI GGTCTC 1 cut(s) 1949
BsrBI CCGCTC 1 cut(s) 1435
BsrDI GCAATG 2 cut(s) 1276, 1601
BsrGI TGTACA 1 cut(s) 1418
BsrI ACTGG 4 cut(s) 349, 816, 1766, 1830
BssECI CCNNGG 2 cut(s) 218, 288
BssNI GRCGYC 1 cut(s) 1438
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 2 cut(s) 220, 1490
Bst4CI ACNGT 7 cut(s) 703, 1195, 1508, 1685, 1737, 1857, 1960
Bst6I CTCTTC 6 cut(s) 21, 52, 372, 396, 432, 1269
BstACI GRCGYC 1 cut(s) 1438
BstAUI TGTACA 1 cut(s) 1418
BstBI TTCGAA 1 cut(s) 1281
BstC8I GCNNGC 4 cut(s) 522, 1133, 1606, 1924
BstDEI CTNAG 9 cut(s) 44, 162, 255, 411, 465, 485, 504, 1110, 1562
BstF5I GGATG 7 cut(s) 421, 517, 555, 805, 852, 1726, 1779
BstFNI CGCG 1 cut(s) 1785
BstH2I RGCGCY 1 cut(s) 1441
BstHHI GCGC 1 cut(s) 1440
BstMAI GTCTC 6 cut(s) 36, 104, 329, 376, 537, 1949
BstMWI GCNNNNNNNGC 7 cut(s) 1132, 1249, 1401, 1410, 1605, 1923, 1947
BstNI CCWGG 2 cut(s) 220, 1490
BstNSI RCATGY 4 cut(s) 735, 1177, 1181, 1926
BstSCI CCNGG 3 cut(s) 218, 1488, 1599
BstSLI GKGCMC 1 cut(s) 982
BstUI CGCG 1 cut(s) 1785
BstV1I GCAGC 3 cut(s) 1067, 1110, 1982
BstV2I GAAGAC 2 cut(s) 1166, 1383
BstX2I RGATCY 1 cut(s) 1557
BstYI RGATCY 1 cut(s) 1557
BsuRI GGCC 2 cut(s) 12, 576
BtrI CACGTC 1 cut(s) 977
BtsCI GGATG 7 cut(s) 421, 517, 555, 805, 852, 1726, 1779
BtsIMutI CAGTG 1 cut(s) 1570
BtuMI TCGCGA 1 cut(s) 1785
BveI ACCTGC 1 cut(s) 1094
Cac8I GCNNGC 4 cut(s) 522, 1133, 1606, 1924
CaiI CAGNNNCTG 2 cut(s) 344, 1109
CfoI GCGC 1 cut(s) 1440
Cfr13I GGNCC 3 cut(s) 850, 944, 1985
Csp6I GTAC 5 cut(s) 1191, 1419, 1504, 1669, 1847
CviQI GTAC 5 cut(s) 1191, 1419, 1504, 1669, 1847
DdeI CTNAG 9 cut(s) 44, 162, 255, 411, 465, 485, 504, 1110, 1562
DinI GGCGCC 1 cut(s) 1439
DrdI GACNNNNNNGTC 2 cut(s) 1178, 1860
DseDI GACNNNNNNGTC 2 cut(s) 1178, 1860
Eam1104I CTCTTC 6 cut(s) 21, 52, 372, 396, 432, 1269
EarI CTCTTC 6 cut(s) 21, 52, 372, 396, 432, 1269
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 1949
Eco47I GGWCC 3 cut(s) 850, 944, 1985
Eco57I CTGAAG 2 cut(s) 152, 291
Eco88I CYCGRG 2 cut(s) 1316, 1623
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 218, 1488
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 2 cut(s) 1157, 1179
EgeI GGCGCC 1 cut(s) 1439
EheI GGCGCC 1 cut(s) 1439
ErhI CCWWGG 1 cut(s) 288
FalI AAGNNNNNCTT 4 cut(s) 369, 401, 1124, 1156
FaqI GGGAC 1 cut(s) 201
FauI CCCGC 1 cut(s) 1248
FbaI TGATCA 3 cut(s) 496, 1548, 1708
Fnu4HI GCNGC 4 cut(s) 1081, 1124, 1436, 1996
FokI GGATG 7 cut(s) 428, 524, 542, 812, 859, 1733, 1786
Fsp4HI GCNGC 4 cut(s) 1081, 1124, 1436, 1996
FspBI CTAG 1 cut(s) 1866
GlaI GCGC 1 cut(s) 1439
GluI GCNGC 4 cut(s) 1081, 1124, 1436, 1996
GsaI CCCAGC 2 cut(s) 1432, 1921
GsuI CTGGAG 1 cut(s) 1472
HaeII RGCGCY 1 cut(s) 1441
HaeIII GGCC 2 cut(s) 12, 576
HapII CCGG 3 cut(s) 35, 1024, 1601
HhaI GCGC 1 cut(s) 1440
Hin1I GRCGYC 1 cut(s) 1438
Hin6I GCGC 1 cut(s) 1438
HinP1I GCGC 1 cut(s) 1438
HincII GTYRAC 2 cut(s) 729, 1843
HindII GTYRAC 2 cut(s) 729, 1843
HpaII CCGG 3 cut(s) 35, 1024, 1601
HphI GGTGA 5 cut(s) 68, 141, 344, 692, 990
Hpy166II GTNNAC 7 cut(s) 729, 980, 1339, 1421, 1733, 1843, 1849
Hpy188I TCNGA 8 cut(s) 171, 256, 271, 475, 1171, 1216, 1330, 1800
Hpy8I GTNNAC 7 cut(s) 729, 980, 1339, 1421, 1733, 1843, 1849
Hpy99I CGWCG 1 cut(s) 1993
HpyAV CCTTC 5 cut(s) 102, 162, 569, 587, 1181
HpyCH4III ACNGT 7 cut(s) 703, 1195, 1508, 1685, 1737, 1857, 1960
HpyCH4IV ACGT 4 cut(s) 976, 1285, 1495, 1845
HpyF10VI GCNNNNNNNGC 7 cut(s) 1132, 1249, 1401, 1410, 1605, 1923, 1947
HpyF3I CTNAG 9 cut(s) 44, 162, 255, 411, 465, 485, 504, 1110, 1562
HpySE526I ACGT 4 cut(s) 976, 1285, 1495, 1845
Hsp92I GRCGYC 1 cut(s) 1438
HspAI GCGC 1 cut(s) 1438
KasI GGCGCC 1 cut(s) 1437
Kpn2I TCCGGA 1 cut(s) 1023
KpnI GGTACC 1 cut(s) 1194
Ksp22I TGATCA 3 cut(s) 496, 1548, 1708
LmnI GCTCC 4 cut(s) 349, 616, 1432, 1482
Lsp1109I GCAGC 3 cut(s) 1067, 1110, 1982
LweI GCATC 5 cut(s) 409, 718, 994, 1092, 1207
MaeI CTAG 1 cut(s) 1866
MaeII ACGT 4 cut(s) 976, 1285, 1495, 1845
MaeIII GTNAC 5 cut(s) 441, 680, 738, 1028, 1195
MbiI CCGCTC 1 cut(s) 1435
MfeI CAATTG 1 cut(s) 1259
MflI RGATCY 1 cut(s) 1557
MhlI GDGCHC 1 cut(s) 982
Mly113I GGCGCC 1 cut(s) 1438
MlyI GAGTC 6 cut(s) 38, 305, 326, 539, 830, 1328
MmeI TCCRAC 4 cut(s) 777, 813, 946, 1127
Mph1103I ATGCAT 2 cut(s) 1157, 1179
MroI TCCGGA 1 cut(s) 1023
MroXI GAANNNNTTC 1 cut(s) 1578
MseI TTAA 5 cut(s) 807, 924, 957, 1881, 1893
MslI CAYNNNNRTG 1 cut(s) 1446
MspA1I CMGCKG 1 cut(s) 484
MspI CCGG 3 cut(s) 35, 1024, 1601
MspR9I CCNGG 3 cut(s) 220, 1490, 1601
MunI CAATTG 1 cut(s) 1259
Mva1269I GAATGC 2 cut(s) 412, 1082
MvaI CCWGG 2 cut(s) 220, 1490
MvnI CGCG 1 cut(s) 1785
MwoI GCNNNNNNNGC 7 cut(s) 1132, 1249, 1401, 1410, 1605, 1923, 1947
NarI GGCGCC 1 cut(s) 1438
NciI CCSGG 1 cut(s) 1601
NlaIV GGNNCC 2 cut(s) 1192, 1439
NmuCI GTSAC 1 cut(s) 680
NruI TCGCGA 1 cut(s) 1785
NsiI ATGCAT 2 cut(s) 1157, 1179
NspI RCATGY 4 cut(s) 735, 1177, 1181, 1926
NspV TTCGAA 1 cut(s) 1281
PaeI GCATGC 1 cut(s) 1926
PaeR7I CTCGAG 1 cut(s) 1316
PaqCI CACCTGC 1 cut(s) 1094
PceI AGGCCT 1 cut(s) 576
PciI ACATGT 1 cut(s) 731
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 2 cut(s) 412, 1082
PdmI GAANNNNTTC 1 cut(s) 1578
PfeI GAWTC 7 cut(s) 172, 223, 592, 1292, 1586, 1777, 1801
PflMI CCANNNNNTGG 1 cut(s) 883
PfoI TCCNGGA 1 cut(s) 1488
PkrI GCNGC 4 cut(s) 1082, 1125, 1437, 1997
PleI GAGTC 6 cut(s) 37, 304, 325, 538, 830, 1327
PluTI GGCGCC 1 cut(s) 1441
PpsI GAGTC 6 cut(s) 37, 304, 325, 538, 830, 1327
PscI ACATGT 1 cut(s) 731
Psp1406I AACGTT 1 cut(s) 1285
Psp6I CCWGG 2 cut(s) 218, 1488
PspFI CCCAGC 2 cut(s) 1428, 1917
PspGI CCWGG 2 cut(s) 218, 1488
PspN4I GGNNCC 2 cut(s) 1192, 1439
PspPI GGNCC 3 cut(s) 850, 944, 1985
PspXI VCTCGAGB 1 cut(s) 1316
PstNI CAGNNNCTG 2 cut(s) 344, 1109
PsuI RGATCY 1 cut(s) 1557
PvuII CAGCTG 1 cut(s) 484
RruI TCGCGA 1 cut(s) 1785
RsaI GTAC 5 cut(s) 1192, 1420, 1505, 1670, 1848
RsaNI GTAC 5 cut(s) 1191, 1419, 1504, 1669, 1847
RseI CAYNNNNRTG 1 cut(s) 1446
SaqAI TTAA 5 cut(s) 807, 924, 957, 1881, 1893
SatI GCNGC 4 cut(s) 1081, 1124, 1436, 1996
Sau96I GGNCC 3 cut(s) 850, 944, 1985
ScaI AGTACT 2 cut(s) 1505, 1670
SchI GAGTC 6 cut(s) 38, 305, 326, 539, 830, 1328
ScrFI CCNGG 3 cut(s) 220, 1490, 1601
SduI GDGCHC 1 cut(s) 982
SfaNI GCATC 5 cut(s) 409, 718, 994, 1092, 1207
SfoI GGCGCC 1 cut(s) 1439
Sfr274I CTCGAG 1 cut(s) 1316
SfuI TTCGAA 1 cut(s) 1281
SinI GGWCC 3 cut(s) 850, 944, 1985
SlaI CTCGAG 1 cut(s) 1316
SmiMI CAYNNNNRTG 1 cut(s) 1446
SmlI CTYRAG 1 cut(s) 1316
SmoI CTYRAG 1 cut(s) 1316
SphI GCATGC 1 cut(s) 1926
SseBI AGGCCT 1 cut(s) 576
SsiI CCGC 6 cut(s) 189, 195, 1241, 1435, 1531, 1692
SspDI GGCGCC 1 cut(s) 1437
SspMI CTAG 1 cut(s) 1866
StuI AGGCCT 1 cut(s) 576
StyD4I CCNGG 3 cut(s) 218, 1488, 1599
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 7 cut(s) 703, 1195, 1508, 1685, 1737, 1857, 1960
TaiI ACGT 4 cut(s) 979, 1288, 1498, 1848
TaqI TCGA 4 cut(s) 226, 580, 1281, 1317
TaqII GACCGA 1 cut(s) 2002
TatI WGTACW 3 cut(s) 1418, 1503, 1668
TauI GCSGC 1 cut(s) 1438
TfiI GAWTC 7 cut(s) 172, 223, 592, 1292, 1586, 1777, 1801
Tru1I TTAA 5 cut(s) 807, 924, 957, 1881, 1893
Tru9I TTAA 5 cut(s) 807, 924, 957, 1881, 1893
TscAI CASTG 1 cut(s) 1570
TseFI GTSAC 1 cut(s) 680
TseI GCWGC 3 cut(s) 1080, 1123, 1995
Tsp45I GTSAC 1 cut(s) 680
TspGWI ACGGA 1 cut(s) 1866
TspRI CASTG 1 cut(s) 1570
Van91I CCANNNNNTGG 1 cut(s) 883
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 3 cut(s) 850, 944, 1985
XapI RAATTY 3 cut(s) 643, 810, 1789
XceI RCATGY 4 cut(s) 735, 1177, 1181, 1926
XhoI CTCGAG 1 cut(s) 1316
XmnI GAANNNNTTC 1 cut(s) 1578
XspI CTAG 1 cut(s) 1866
ZrmI AGTACT 2 cut(s) 1505, 1670
Zsp2I ATGCAT 2 cut(s) 1157, 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.